scholarly journals Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test

1987 ◽  
Vol 29 (6) ◽  
pp. 354-360
Author(s):  
I. T. I. Nojimoto ◽  
S. Hoshino-Shimizu ◽  
T. K. Nagasse-Sugahara ◽  
M. E. Camargo
Keyword(s):  
Relative Efficiency ◽  
Healthy Subjects ◽  
Lens Culinaris ◽  
Test Results ◽  
Immunofluorescence Test ◽  
Igm Antibodies ◽  
Acute Toxoplasmosis

Lectins were labeled with fluorescein and tried as conjugates in the immunofluorescence (IP) test for the detection of IgM antibodies to T. gondii, in the diagnosis of acute toxoplasmosis. This approach was an attempt to find alternative reagents for anti-human IgM fluorescent conjugates (AHIgMFC), which contain quite frequently anaibcdies to toxoplasma, as contaminants, due to natural T. gondii infections among animals used for imunization. Lentil (Lens culinaris) lectin fluorescence conjugates (LcFC) provided most satisfactory results. The evaluation of LcFC carried out in a total of 179 sera from patients with acute and chronic toxoplasmosis, with non-related infections or healthy subjects, gave high values of relative efficiency, co-positivity and co-negativity indices, respectively 0.989, 0.969 and 1.000, in reference to the conventional AHIgMFC. Moreover, three batches of LcFC successively prepared gave reproducible test results. The advantage of LcFC as an alternative reagent for the serodiagnosis of acute toxoplasmosis is supported by practical aspects of its preparation.

Prevalence of Toxoplasma gondii and Toxocara canis among Myositis Patients in Southwest of Iran

2019 ◽  
Vol 20 ◽  
Author(s):  
Jasem Saki ◽  
Karim Mowla ◽  
Reza Arjmand ◽  
Forough Kazemi ◽  
Somayeh Fallahizadeh
Keyword(s):  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Healthy Subjects ◽  
Toxocara Canis ◽  
Control Group ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
High Prevalence ◽  
Acute Toxoplasmosis ◽  
Southwest Of Iran

Introduction: Parasitic myositis is caused by some parasites such as T. gondii and T. canis. So, the aim of the study was to evaluate the prevalence T. gondii and T. canis in patients with myositis and healthy individuals. Methods: A total of 108 samples were randomly selected as the control (54 healthy individuals) and test (54 myositis patients) groups. IgG and IgM antibodies against T. gondii and IgG antibodies against T. canis were measured by the ELISA. The detection of chronic and acute toxoplasmosis was performed by the ELISA IgG avidity. The presence of T. gondii in blood was evaluated by the nested-PCR. Results: Of 108, 33 (30.6%) cases were detected positive for IgG against T. gondii that 19 (35.2%) and 14 (25.9%) were observed in myositis patients and healthy individuals, respectively (P=0.296). Of 19 positive cases, 12 (63.2%) and 7 (36.8%) cases were detected as chronic and acute toxoplasmosis, respectively, while, all positive cases in the control group had chronic toxoplasmosis (P=0.013). One (1.9%) sample was detected positive for anti- Toxoplasma gondii IgM and two (3.7%) samples were found positive for IgG against T. canis by the ELISA that these positive cases were observed only in myositis patients (P=1.000 P=0.495, respectively). B1 T. gondii gene was amplified in 12 (63.2%) and 1 (7.1%) in myositis patients and healthy subjects (P=0.001). Conclusions: Our findings showed that there was a relatively high prevalence of acute toxoplasmosis in myositis patients in comparison with the control subjects in southwest of Iran.

scholarly journals Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies

1989 ◽  
Vol 31 (4) ◽  
pp. 279-285 ◽  
Author(s):  
M.E. Camargo ◽  
Maria Emilia G. Moura ◽  
P.G. Leser
Keyword(s):  
Simple Procedure ◽  
Low Cost ◽  
Protein A ◽  
Developed Countries ◽  
Serum Samples ◽  
Serum Dilution ◽  
Specific Antibodies ◽  
Igm Antibodies ◽  
Diagnostic Capability ◽  
Acute Toxoplasmosis

In search of an efficient but simple, low cost procedure for the serodiagnosis of Toxoplasmosis, especially suited for routine laboratories facing technical and budget limitations as in less developed countries, the diagnostic capability of Hematoxo® , an hemagglutination test for toxoplasmosis, was evaluated in relation to a battery of tests including IgG- and IgM-immunofluorescence tests, hemagglutination and an IgM-capture enzymatic assay. Detecting a little as 5 I.U. of IgG antitoxoplasma antibodies, Hematoxo® showed a straight agreement as to reactivity and non-reactivity for the 443 non-reactive and the 387 reactive serum samples, included in this study. In 23 cases presenting a serological pattern of acute toxoplasmosis and showing IgM antibodies, Hematoxo® could detect IgM antibodies in 18, indicated by negativation or a significant decrease in titers as a result of treating samples with 2-mercapto-ethanol. However, a neat increase in sensitivity for IgM specific antibodies could be achieved by previously removing IgG from the sample, as demonstrated in a series of acute toxoplasmosis sera. A simple procedure was developed for this purpose, by reconstituting a lyophilized suspension of Protein A - rich Staphylococcus with the lowest serum dilution to be tested. Of low cost and easy to perform, Hematoxo® affords not only a practical qualitative procedure for screening reactors and non-reactors, as in prenatal services, but also quantitative assays that permit to titrate antibodies as well as to identify IgM antibodies.

scholarly journals Evaluation of tubal and peritoneal factors in chlamydia positive infertile women by laparoscope

2017 ◽  
Vol 7 (2) ◽  
pp. 54-58
Author(s):  
Nurjahan Begum ◽  
Fawzia Hussain ◽  
Farzana Deeba ◽  
Shaheen Ara Anwary ◽  
Parveen Sultana ◽  
...  
Keyword(s):  
Fallopian Tubes ◽  
Test Results ◽  
Immunofluorescence Test ◽  
Common Cause ◽  
Positive Group ◽  
Chlamydia Trachomatis Infection ◽  
Infertile Women ◽  
Antibody Titres ◽  
The Difference ◽  
The Relationship

Background: Abnormalities or damage to the fallopian tube interferes with fertility and is responsible for abnormal implantation (eg, ectopic pregnancy). Obstruction of the distal end of the fallopian tubes results in accumulation of the normally secreted tubal fluid, creating distention of the tube with subsequent damage of the epithelial cilia (hydrosalpinx). Genital Chlamydia trachomatis infection has a worldwide distribution6 and is now recognized as the single most common cause of tubal peritoneal damage. The study explores the relationship between serum chlamydia antibody titres (CATs) and detection of tubal damage in infertile women.Objective: To Evaluation of tubal and peritonial factors in chlamydia positive infertile women by laparoscope.Methodology: The tubal status and pelvic findings in 138 women underwent laparoscopy for infertility were related to CAT, which was measured using the whole-cell inclusion immunofluorescence test. RESULTS: A total of 138 infertile women who underwent laparoscopic investigation for infertility were identified and they were divided in two groups, on the bsis of presence is absence Chlamydia positive (n=69) and Chlamydia Negative (n=69). Demographic status were almost similar between two groups, however service holder was found significantly higher in Chlamydia positive group (17 vs. 7). Tubal block was found in 44(63.7%) in Chlamydia positive and 37(53.6%) in Chlamydia negative. The difference was statistically significant (p<0.01) between two groups. Site of block & hydrosalpinges was almost similar between two groups. POD was completely obliterated in 10(14.5%) in Chlamydia positive and 3(4.3%) in Chlamydia negative. The difference was statistically significant (p<0.05) between two groups.Conclusion: Chlamydia serology is useful mainly as a screening test for the likelihood of tubal damage in infertile women and may facilitate decisions on which women should proceed with further investigations without delay.J Shaheed Suhrawardy Med Coll, December 2015, Vol.7(2); 54-58

scholarly journals IgM and IgA Antibody Responses in 12 Cases of Human Acquired Toxoplasmosis

1997 ◽  
Vol 39 (6) ◽  
pp. 327-332 ◽  
Author(s):  
Emília E. H. TAKAHASHI ◽  
Cláudio L. ROSSI
Keyword(s):  
Serological Test ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Iga Antibodies ◽  
Direct Elisa ◽  
Acute Toxoplasmosis ◽  
Antibody Levels ◽  
Acquired Toxoplasmosis

The persistence, in some subjects, of specific IgM antibodies to Toxoplasma gondii for several months after the acute phase of infection has complicated the interpretation of serological test results for toxoplasmosis. Several reports have emphasized the value of the detection of Toxoplasma-specific IgA antibodies for the diagnosis of acute toxoplasmosis. In this article, we report the follow-up profiles of Toxoplasma-specific IgM and IgA antibodies in serum samples obtained from 12 patients at various intervals after the onset of the clinical manifestations of infection. IgM antibodies were detected by the indirect immunofluorescence (IIF) test, antibody capture enzyme-linked immunosorbent assay (cELISA) and enzyme-mediated chemilluminescent technique (CmL). IgA antibodies were quantified by the direct ELISA (dELISA) and cELISA procedures. As defined by the manufacturer of the cELISA test for IgA used, most patients with acute toxoplasmosis have antibody levels > 40 arbritary units per ml (AU/ml). At values > 40 AU/ml, the cELISA for IgA detected significant antibody levels for a shorter time than the other techniques used for IgM and IgA detection. However, IgA levels <FONT FACE="Symbol">£</font> 40 AU/ml do not exclude the possibility of acute toxoplasmosis since such levels can be reached very soon after infection with T. gondii. The results obtained in the present study show that the serological diagnosis of acute toxoplasmosis may not be such an easy task. Our data suggest that use of the IgA-cELISA concomitantly with IgM antibody screening could permit, in some circumstances, a more efficient diagnosis of acute acquired toxoplasmosis

scholarly journals Pseudothrombocytopenia: an immunologic study on platelet antibodies dependent on ethylene diamine tetra-acetate

Blood ◽  
1982 ◽  
Vol 59 (1) ◽  
pp. 157-161 ◽  
Author(s):  
JG Pegels ◽  
EC Bruynes ◽  
CP Engelfriet ◽  
AE von dem Borne
Keyword(s):  
Ethylene Diamine ◽  
Igg Antibodies ◽  
Immunofluorescence Test ◽  
Igm Antibodies ◽  
Platelet Antibodies ◽  
Group Specificity ◽  
Iga Antibodies ◽  
Ethylene Diamine Tetra Acetate

Abstract Antibodies specifically reacting with platelets only in the presence of EDTA, by the platelet immunofluorescence test, were found in the serum of 20 patients with pseudothrombocytopenia due to in vitro EDTA- dependent platelet agglutination. These antibodies reacted optimally at 0–4 degree C. In 19 patients, IgG antibodies were detected; in 8 patients, IgM or IgA antibodies were also found. In one patient, only IgM antibodies were found. In 14 patients, the IgG antibodies were IgG1, but IgG2, IgG3, and IgG4 antibodies were also seen in 7 patients. The reaction of platelets with the antibodies was detectable in the presence of Na2EDTA, the K, Ca, and Mg salts of EDTA, and K2EGTA. F(ab')2 or F(ab') fragments of the IgG antibodies reached as strongly as the intact antibodies, indicating that the reaction is dependent on the antibody-combining site. The EDTA-dependent antibodies did not show platelet-group specificity. However, platelets from patients with Glanzmann disease did not react with the antibodies.

First data on the biological variation and quality specifications for plasma ammonia concentrations in healthy subjects

2016 ◽  
Vol 54 (5) ◽  
Author(s):  
Fatma Ucar ◽  
Gonul Erden ◽  
Seyda Ozdemir ◽  
Nurgul Ozcan ◽  
Erdem Bulut ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Plasma Sample ◽  
Biological Variation ◽  
Test Results ◽  
Healthy Individuals ◽  
Variation Data ◽  
Quality Specifications ◽  
Collection Period ◽  
First Time ◽  
Analytical Variation

AbstractBackground:Most of the factors causing preanalytical and analytical variation in ammonia measurement have been identified. Biological variation data for ammonia is still lacking. We therefore estimated the components of biological variation (within-subject=CVMethods:Blood samples from 20 healthy subjects were collected in K2EDTA tubes daily over a period of 4 consecutive days from each subject. Each plasma sample was split into two aliquots; one was immediately analyzed as the samples were collected and the other was stored –80 °C until testing at the end of the collection period and analyzed at once in one analytical run. All samples were analyzed in duplicate. Estimations were calculated according to Fraser and Harris methods.Results:CVConclusions:The present study for the first time described the components of biological variation for ammonia in healthy individuals. These data regarding biological variation of ammonia could be useful for a better evaluation of ammonia test results in clinical interpretation and for determining quality specifications based on biological variation.

Motor Free Visual Perceptual Test: Impact of Vertical Answer Cards Position on Performance of Adults with Hemispatial Visual Neglect

1995 ◽  
Vol 15 (4) ◽  
pp. 223-236 ◽  
Author(s):  
Louisette Merrier ◽  
Réjean Hébert ◽  
Louise Gauthier
Keyword(s):  
Brain Damage ◽  
Healthy Subjects ◽  
Vertical Position ◽  
Test Results ◽  
Visual Neglect ◽  
Assessment Tests ◽  
Right Brain ◽  
Perceptual Test ◽  
Perceptual Assessment ◽  
Test Retest Reliability

Patients who have hemispatial visual neglect (HVN) have difficulty directing their attention toward the visual field contralateral to the side of the lesion. This affects their performance on perceptual assessment tests and masks related visual perceptual deficits. The aim of this study was to verify whether a vertical position of the answer cards of the Motor Free Visual Perceptual Test (MVPT) could overcome this problem. Three groups of subjects were involved in the study: 15 healthy subjects, 15 subjects who had right brain damage without HVN, and 9 subjects who had right brain damage with HVN. The presence of HVN was established by positive test results for at least one of the two tests used, the Albert Test and the Bells Test. The subjects performed the MVPT twice—once in its standard version, and once in a modified version in which the answer cards were presented in a vertical position. The equivalence between the two forms was confirmed with healthy subjects and subjects with right brain damage without HVN. Tor the subjects who had HVN, the vertical positioning of the answer cards caused significantly less interference (p < .05). The test-retest reliability coefficient for the two versions of the MVPT was 0.92–0.94.

scholarly journals Indirect immunofluorescence test in new world Leishmaniasis: serological and clinical relation-ship

1988 ◽  
Vol 83 (3) ◽  
pp. 347-355 ◽  
Author(s):  
Sergio C. F. Mendonça ◽  
Wilson J. S. Souza ◽  
Marise P. Nunes ◽  
Mauro C. A. Marzochi ◽  
Sergio G. Coutinho
Keyword(s):  
Visceral Leishmaniasis ◽  
Cutaneous Leishmaniasis ◽  
Indirect Immunofluorescence ◽  
Healthy Subjects ◽  
Endemic Area ◽  
New World ◽  
Leishmania Braziliensis ◽  
Immunofluorescence Test ◽  
Control Groups ◽  
Indirect Immunofluorescence Test

The indirect immunofluorescence test (IF) for anti-Leishmania antibodies (IgC and IgM) was performed with sera form the following groups of individuals: 214 cutaneous leishmaniasis patients, 28 healthy subjects with positive Montenegro's skin test (MST), 29 healthy subjects with negativeMST and 16 visceral leishmaniasis patients. The first four groups came from a suburban area of Rio de Janeiro (Jacarepaguá) where cutaneous leishmaniasis caused by Leishmania braziliensis braziliensis is endemic. It was observed that IF-IgM titers were significantly higher amongst the cutaneous leishmaniasis patients with less than four months of disease as compared to those with longer periods and that IF-IgG titers were significantly higher in patients with multiple lesions as compared to those with single lesions. The visceral leishmaniasis patients had IF-IgG titers significantly higher than those from cutaneous leishmaniasis patients. A group of 28 individuals selected amongst the 214 cutaneous leishmaniasis patients had their IF-titers (IgG and IgM) compared to those of the two control groups of healthy subjects from the endemic area, respectively with positive and negative MST. Significantly higher titers of IF-IgG and IF-IgM were found in the group with active disease. The same group of patients showed IF-IgG titers significantly lower at the end of the antimonial therapy than those observed during this tratment.

scholarly journals Anti-Hepatitis A Virus Immunoglobulin M Antibodies in Urine Samples for Rapid Diagnosis of Outbreaks

2003 ◽  
Vol 10 (3) ◽  
pp. 492-494 ◽  
Author(s):  
Licel de los Angeles Rodríguez Lay ◽  
Osmany Larralde Díaz ◽  
Raiza Martínez Casanueva ◽  
Aidonis Gutiérrez Moreno
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Rapid Diagnosis ◽  
Immunoglobulin M ◽  
Urine Samples ◽  
Test Results ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Urine And Serum

ABSTRACT The main goal of this study was to test the feasibility of using urine for diagnosing hepatitis A virus (HAV) infections. A correlation of 90.78% between the test results of urine and serum samples was obtained. Four outbreaks of hepatitis A were confirmed by testing only urine samples. The levels of anti-HAV immunoglobulin M (IgM) antibodies in urine samples remained stable during 6 months of storage at −70°C but decreased when the samples were stored at 4°C. The results of tests of samples obtained 2 and 6 months after infection suggested that IgM levels decline more rapidly in urine than in serum.

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