scholarly journals Factors associated with Leishmania chagasi infection in domestic dogs from Teresina, State of Piauí, Brazil

2012 ◽  
Vol 45 (4) ◽  
pp. 480-484 ◽  
Author(s):  
João Pereira da Silva ◽  
Guilherme Loureiro Werneck ◽  
Emanuelle Cardoso Macedo ◽  
Hanny de Carvalho ◽  
Maria do Socorro Pires e Cruz
Keyword(s):  
Visceral Leishmaniasis ◽  
Control Program ◽  
Antibody Test ◽  
Domestic Dogs ◽  
Leishmania Chagasi ◽  
Serological Tests ◽  
Factors Associated ◽  
Human Visceral Leishmaniasis ◽  
History Of ◽  
The City

INTRODUCTION: Many studies have evaluated risk factors for human visceral leishmaniasis, but few have focused on the infection among dogs. The objective of this study was to assess the association between peridomestic socioeconomic and environmental factors and the presence of dogs seropositive for Leishmania chagasi in the City of Teresina, Brazil. METHODS: This case-control study was based on the results of a routine seroepidemiological survey among domestic dogs carried out in 2007. Serological tests were performed by means of indirect immunofluorescence antibody test. All dwellings in which at least one seropositive dog was detected were considered cases, and controls were a random sample of dwellings in which only seronegative dogs were identified. Associations between variables were expressed as odds ratios (OR) and their respective 95% confidence intervals (95%CI) estimated using multivariate logistic regression. RESULTS: Dwellings with a history of dogs removed by the visceral leishmaniasis control program in the last 12 months had five-fold higher odds of having at least one seropositive dog as compared with dwellings having no history of dog removal (OR = 5.19; 95%CI = 3.20-8.42). Dwellings with cats had 58% increased odds of dog infection as compared with those having no cats (OR = 1.58; 95%CI = 1.01-2.47). CONCLUSIONS: Identification of factors associated with canine visceral leishmaniasis might be used for the delimitation of areas of higher risk for human visceral leishmaniasis, since infection in dogs generally precedes the appearance of human cases.

Are backyard characteristics relevant factors for the occurrence of human visceral leishmaniasis in Central-Western Brazil?

2019 ◽  
Vol 114 (4) ◽  
pp. 276-283 ◽  
Author(s):  
João Gabriel Guimarães Luz ◽  
Amanda Gabriela Carvalho ◽  
Danilo Bueno Naves ◽  
João Victor Leite Dias ◽  
Cor Jesus Fernandes Fontes
Keyword(s):  
Visceral Leishmaniasis ◽  
Conditional Logistic Regression ◽  
Control Measures ◽  
Retrospective Case ◽  
Conditional Logistic Regression Analysis ◽  
Factors Associated ◽  
Human Visceral Leishmaniasis ◽  
History Of ◽  
And Control ◽  
Relevant Factors

ABSTRACT Background This retrospective case-control study aimed to investigate the factors associated with the occurrence of human visceral leishmaniasis (VL) in the municipality of Rondonópolis, which is a highly endemic area for VL in Central-Western Brazil. Methods All VL cases reported in Rondonópolis from 2011 to 2016 were included. For each case, two neighborhood- and age-matched controls with the absence of past and present history of VL and negative rk39-based immunochromatographic test, were recruited. Data were retrospectively collected through interviews that assessed socioeconomic aspects, housing features, backyard characteristics, presence of animals and daily habits. A multivariate conditional logistic regression analysis was used to identify factors associated with human VL. Results During the study period, 81 VL cases were reported in Rondonópolis, of which 37 (45.7%) were included in this study. Seventy controls were also included. The variables associated with VL occurrence were the outdoor location of a bathroom and/or kitchen separated from the rest of the house (OR 7.8; 95% CI 2.1 to 29.5), the presence of a vegetable garden (OR 5.3; 95% CI 1.3 to 21.2) and the presence of decaying fruits (OR 10.7; 95% CI 2.0 to 52.1) in the backyard. Conclusions Our findings suggest an association between backyard characteristics and VL. This may be useful in guiding preventive and control measures against zoonotic VL worldwide.

scholarly journals Canine visceral leishmaniasis in Barra de Guaratiba, Rio de Janeiro, Brazil: assessment of risk factors

2003 ◽  
Vol 45 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Maria Alice A. Cabrera ◽  
Adelzon A. Paula ◽  
Luis Antonio B. Camacho ◽  
Mauro Célio A. Marzochi ◽  
Samanta C. Xavier ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Rio De Janeiro ◽  
Antibody Test ◽  
Control Measures ◽  
Lutzomyia Longipalpis ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
The City ◽  
Immunofluorescent Antibody Test ◽  
Predictor Factors

Barra de Guaratiba is a coastal area of the city of Rio de Janeiro where American visceral leishmaniasis (AVL) is endemic. Although control measures including killing of dogs and use of insecticides have been applied at this locality, the canine seroprevalence remains at 25% and during 1995 and 1997 eight autochthonous human cases were notified. In order to evaluate factors related to the increase of the risk for Leishmania (Leishmania) chagasi infection in dogs we have screened 365 dogs by anti-Leishmania immunofluorescent antibody test (IFAT) and captured sandflies in the domestic and peridomestic environment. Some variables related to the infection were assessed by uni- and multivariate analysis. The distance of the residence from the forest border, its altitude and the presence of the opossum Didelphis marsupialis in the backyard, were found predictor factors for L. (L.) chagasi infection in dogs in Barra de Guaratiba. The presence of Lutzomyia longipalpis in the peridomestic environment indicates the possibility of appearence of new human cases. Our data also suggest the presence of a sylvatic enzootic cycle at this locality.

scholarly journals Occurrence of Lutzomyia longipalpis (Phlebotominae) and canine visceral leishmaniasis in a rural area of Ilha Solteira, SP, Brazil

2014 ◽  
Vol 23 (4) ◽  
pp. 456-462 ◽  
Author(s):  
Julio Cesar Pereira Spada ◽  
Diogo Tiago da Silva ◽  
Kennya Rozy Real Martins ◽  
Lílian Aparecida Colebrusco Rodas ◽  
Maria Luana Alves ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Rural Area ◽  
Antibody Test ◽  
Lutzomyia Longipalpis ◽  
Climatic Data ◽  
Canine Visceral Leishmaniasis ◽  
Serological Tests ◽  
Relative Air Humidity ◽  
Control And Prevention ◽  
One Year

This study aimed to investigate the occurrence of Lutzomyia longipalpis and also the canine visceral leishmaniasis (CVL) in a rural area of Ilha Solteira, state of São Paulo. Blood samples were collected from 32 dogs from different rural properties (small farms) and were analyzed by ELISA and the indirect immunofluorescence antibody test (IFAT) in order to diagnose CVL. From these serological tests, 31.25% of the dogs were positive for CVL and these were distributed in 66.7% (8/12) of the rural properties, which were positive for L. longipalpis. CDC (Center for Disease Control and Prevention) light traps were installed in 12 properties (one per property) and insects were caught on three consecutive days per month for one year. L. longipalpis was present on 100% of the rural properties visited, at least once during the twelve-month interval, totaling 64 males and 25 females. The insects were more numerous after the peak of the rain, but the association between prevalence of peridomestic vectors and the climatic data (precipitation, relative air humidity and temperature) and the occurrences of CVL among dogs on each rural property were not statistical significant (p <0.05). However, the occurrence of CVL cases in dogs and the presence of L. longipalpis indicate that more attention is necessairy for the control of this disease in the rural area studied.

scholarly journals Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Daniel Moreira de Avelar ◽  
Débora Moreira Carvalho ◽  
Ana Rabello
Keyword(s):  
Visceral Leishmaniasis ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Health Concern ◽  
Serological Tests ◽  
Blood Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Human Visceral Leishmaniasis ◽  
Highly Sensitive ◽  
Sensitivity Specificity

Visceral leishmaniasis (VL) is considered a major public health concern in Brazil and several regions of the world. A recent advance in the diagnosis of infectious diseases was the development of loop-mediated isothermal amplification (LAMP). The aim of this study was to develop and evaluate a new LAMP assay for detection of K26 antigen-coding gene of L. donovani complex. A total of 219 blood samples of immunocompetent patients, including 114 VL cases and 105 non-VL cases, were analyzed for the diagnosis of VL in the present study. Diagnostic accuracy was calculated against a combination of parasitological and/or serological tests as a reference standard. The results were compared to those of kDNA Leishmania-PCR. The detection limit for the K26-Lamp assay was 1fg L. infantum purified DNA and 100 parasites/mL within 60 min of amplification time with visual detection for turbidity. The assay was specific for L. donovani complex. Sensitivity, specificity, and accuracy were 98.2%, 98.1%, and 98.2%, respectively, for K26-LAMP and 100%, 100%, and 100%, respectively, for kDNA Leishmania-PCR. Excellent agreement was observed between K26-LAMP and kDNA Leishmania-PCR assays (K = 0.96). A highly sensitive and specific LAMP assay targeting K26 antigen-coding gene of L. donovani complex was developed for diagnosis in peripheral blood samples of VL patients.

scholarly journals Prokaryotic Expression and Antigenic Characterization of Three Recombinant Leishmania Antigens for Serological Diagnosis of Canine Leishmaniasis

2003 ◽  
Vol 10 (6) ◽  
pp. 1153-1156 ◽  
Author(s):  
S. Rosati ◽  
M. Ortoffi ◽  
M. Profiti ◽  
A. Mannelli ◽  
W. Mignone ◽  
...  
Keyword(s):  
Antibody Test ◽  
Canine Leishmaniasis ◽  
Gene Fragment ◽  
Leishmania Chagasi ◽  
Serological Tests ◽  
Reading Frame ◽  
Antigenic Characterization ◽  
Immunofluorescent Antibody Test ◽  
Immunodominant Epitopes

ABSTRACT Three recombinant antigens of Leishmania chagasi (= L. infantum) were expressed in prokaryotic systems and evaluated (using a panel of dog sera characterized by parasitological and serological immunofluorescent antibody test [IFAT] techniques) as diagnostic markers of infection. The whole open reading frame encoding K9, the gene fragment encoding the repetitive sequence of K26, and the 3′-terminal gene fragment encoding a single 39-amino-acid subunit of the kinesin-related protein K39 (K39sub) were amplified from L. infantum DNA and cloned into a pGEX-2T expression vector in frame with glutathione S-transferase (GST). The sensitivity and specificity of enzyme-linked immunosorbent assays (ELISAs) using K26 as an antigen (evaluated with sera from 20 parasitologically positive and 20 parasitologically negative dogs) were both 100% (95% confidence interval [CI] = 83.2 to 100). When K9 and K39sub were used, sensitivity was 95% (95% CI = 75.1 to 99.9) and specificity was 100% (95% CI = 83.2 to 100). Using 182 field sera, a good agreement was found between the recombinant K26 ELISA and IFAT (K = 0.92; 95% CI = 0.86 to 0.98) results and between the K9 and K39sub ELISA (used in parallel) and IFAT (K = 0.87; 95% CI = 0.80 to 0.95) results. The results demonstrate that each antigen carries immunodominant epitopes and that their combination may further increase the sensitivity of currently available serological tests.

scholarly journals Cost-effectiveness of serological tests for human visceral leishmaniasis in the Brazilian scenario

2020 ◽  
Vol 14 (10) ◽  
pp. e0008741
Author(s):  
Mariana Lourenço Freire ◽  
Aline de Souza ◽  
Gláucia Cota ◽  
Ana Rabello ◽  
Tália Machado de Assis
Keyword(s):  
Cost Effectiveness ◽  
Visceral Leishmaniasis ◽  
Serological Tests ◽  
Human Visceral Leishmaniasis

Risk Factors Associated with Human Visceral Leishmaniasis in an Urban Area of Bahia, Brazil

2016 ◽  
Vol 16 (6) ◽  
pp. 368-376 ◽  
Author(s):  
Zuinara Maia ◽  
Vivian Viana ◽  
Eduardo Muniz ◽  
Larissa Oliveira Gonçalves ◽  
Carlos Maurício Cardeal Mendes ◽  
...  
Keyword(s):  
Risk Factors ◽  
Visceral Leishmaniasis ◽  
Urban Area ◽  
Factors Associated ◽  
Human Visceral Leishmaniasis

scholarly journals Leishmania infection in a population of dogs: an epidemiological investigation relating to visceral leishmaniasis control

2013 ◽  
Vol 22 (4) ◽  
pp. 592-596 ◽  
Author(s):  
Klauber Menezes Penaforte ◽  
Vinícius Silva Belo ◽  
Rafael Gonçalves Teixeira-Neto ◽  
Renata Aparecida Nascimento Ribeiro ◽  
Robson Bruniera de Oliveira ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Regression Models ◽  
Control Strategies ◽  
Control Program ◽  
Leishmania Infection ◽  
Canine Visceral Leishmaniasis ◽  
Epidemiological Investigation ◽  
Logistic Regression Models ◽  
Factors Associated ◽  
Control Actions

Identification of factors associated with Leishmaniainfection in dogs is essential for targeting visceral leishmaniasis control actions. Thus, the present study analyzed some of these factors in a population of dogs in a Brazilian municipality, along with the limitations of control strategies implemented there. The association between the exposure variables and occurrences of infection was analyzed through logistic regression models. The disease control interventions were treated qualitatively. Out of the 755 animals examined, 13.6% (103/755) were seropositive. Of these, 23.3% (24/103) were asymptomatic and 76.7% (79/103) presented at least one clinical sign possibly associated with visceral leishmaniasis. With weak associations, purebred, shorthaired, over 5 years of age, male and large dogs were more prone to infection. The latter two variables formed the final regression model and the association with dog size was statistically significant. The control strategies developed presented limitations and a great number of seronegative dogs was culled. The data presented contribute towards better understanding of the dynamics of infection in canine visceral leishmaniasis and indicate that actions aimed towards adequate implementation of Visceral Leishmaniasis control program in Brazilian endemic areas should be prioritized.

scholarly journals A Novel Sampling Model to Study the Epidemiology of Canine Leishmaniasis in an Urban Environment

2021 ◽  
Vol 8 ◽  
Author(s):  
Lucy A. Parker ◽  
Lucrecia Acosta ◽  
Mariana Noel Gutierrez ◽  
Israel Cruz ◽  
Javier Nieto ◽  
...  
Keyword(s):  
Representative Sample ◽  
Clinical Signs ◽  
Domestic Dogs ◽  
Urban Setting ◽  
Domestic Dog ◽  
Canine Leishmaniasis ◽  
Serological Tests ◽  
Efficient Manner ◽  
Shelter Dogs ◽  
The City

Background: Visceral leishmaniasis (VL) is one of the most important parasitic diseases in the world. The domestic dog is the main reservoir of zoonotic VL and a high prevalence of canine leishmaniasis (CanL) is associated with transmission of infection to humans. Here we describe the methodology used to obtain a rapid and representative sample of domestic dogs in the city of Posadas, Misiones, and compare the prevalence of Leishmania infection with a sample of shelter dogs.Methodology: We used the city land registry to make a random selection of homes and systematically recruited 349 domestic dogs from the selected properties. We also included all dogs from the main canine shelter within the city. Dogs were examined by two experienced veterinarians who recorded the presence of clinical signs common in CanL using a standardized protocol. We extracted a blood sample from each dog and performed four different serological tests to reveal the presence of anti-Leishmania antibodies.Results: After clinical examination, 145 domestic dogs (41.5%) and 63 (90%) shelter dogs had clinical signs compatible with CanL (p &lt; 0.001). The seroprevalence among domestic dogs was 20.1% (95% CI 16.1–24.6) which was significantly lower than among the abandoned dogs (38.6%, 95% CI 27.7–50.6, p &lt; 0.001). The spatial distribution of infected dogs was fairly homogenous throughout the city. Among domestic dogs, we observed a positive association between where the dog slept and presence of anti-Leishmania antibodies (p = 0.034). Of the seropositive domestic dogs 38 (54.4%) were asymptomatic.Conclusions: Our findings demonstrate how seroprevalence results can be highly influenced by sampling methodology. We demonstrate how the land registry can be used to estimate the prevalence of CanL in representative sample of domestic dogs in an urban setting, allowing decision makers to deepen their understanding the epidemiology of CanL in a timely and efficient manner for the development of plans to address both human and canine disease.

scholarly journals Intense transmission of visceral leishmaniasis in a region of northeastern Brazil: a situation analysis after the discontinuance of a zoonosis control program

2021 ◽  
Vol 30 (1) ◽  
Author(s):  
Ranielly Araujo Nogueira ◽  
Maria Gabriela Sampaio Lira ◽  
Sarah Ingrid Pinto Santos ◽  
Renata Mondêgo-Oliveira ◽  
Fábio Henrique Evangelista de Andrade ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Control Program ◽  
Health Department ◽  
Northeastern Brazil ◽  
Canine Leishmaniasis ◽  
Seropositivity Rate ◽  
Human Visceral Leishmaniasis ◽  
Control And Prevention ◽  
Using Data ◽  
Municipal Health

Abstract In São Luís, Maranhão, northeastern Brazil, the notification of visceral leishmaniasis (VL) cases intensified in 1982, showing endemic and epidemic patterns. In this city, the Center for Zoonoses Control (CZC) was an organization in charge of the control and prevention of the disease. However, technical and political reasons have led to a significant decline in the periodicity of its activities. Therefore, in this study we evaluated the epidemiological scenario of human visceral leishmaniasis (HVL) and the prevalence of the disease in dogs after the cessation of the CZC activities, covering the period of 2007 to 2016. The seroprevalence of canine leishmaniasis was determined based on clinical and serological profiles. HVL cases were notified using data provided by the Municipal Health Department of São Luís. A seropositivity rate of 45.8% (p = 0.0001) was found among dogs, 54% (p = 0.374) of which were asymptomatic. As for human cases, there were 415 notifications, with an increase in the incidence of the zoonosis observed during the aforementioned period. Thus, it can be inferred that after the control and surveillance activities were curtailed, there was an increase in the number of seropositive animals in circulation, acting as reservoirs of infection for dogs and humans.

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