scholarly journals Effect of the celexoxib in microscopic changes of the esophageal mucosal of rats induced by esofagojejunostomy

2014 ◽  
Vol 41 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Austry Ferreira de Lima ◽  
Laercio Gomes Lourenço ◽  
Délcio Matos ◽  
Célio Fernando de Sousa Rodrigues
Keyword(s):  
Protective Effect ◽  
Microscopic Analysis ◽  
Distal Segment ◽  
Group Iv ◽  
Esophageal Mucosa ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
The University ◽  
Observation Period

OBJECTIVE: To evaluate the protective effect of celecoxib in the esophageal mucosa in rats undergoing esofagojejunostomy. METHODS: Sixty male Wistar rats from the vivarium of the University of Health Sciences of Alagoas were used for the experiment. The animals were divided into four groups: Group I, 15 rats undergoing esofagojejunostomy with the use of celecoxib postoperatively; Group II, 15 rats undergoing esofagojejunostomy without the use of celecoxib; Group III, 15 rats undergoing celiotomy with bowel manipulation; and Group IV, 15 rats without surgery and using celecoxib. The observation period was 90 days. After the death of the animals, the distal segment of the esophagus was resected and sent for microscopic analysis. RESULTS: esofagojejunostomy caused macroscopic and microscopic esophagitis. Esophagitis was equal in both groups I and II. In groups III and IV esophageal lesions were not developed. CONCLUSIONS: celecoxib had neither protective nor inducing effect on esophagitis, but had a protective effect on dysplasia of the animals of group I.

scholarly journals Effect of concurrent exposure of toxic concentrations of lead and endosulfan on oxidative stress indices in rats

2021 ◽  
Vol 10 (3) ◽  
pp. 192-195
Author(s):  
Ranganathan V ◽  
◽  
Malik JK ◽  
Rao GS ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Group Iv ◽  
Male Rats ◽  
Technical Grade ◽  
Stress Indices ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Toxic Concentrations

The effect of concurrent exposure of toxic concentrations of lead and endosulfan were evaluated on oxidative stress parameters in male wistar rats. Group I served as untreated control whereas Group II received drinking water containing lead as lead acetate @1000 ppm (Pb1000). Group III was exposed to feed containing technical grade endosulfan @ 100 ppm (E100). Group IV was exposed to Pb (1000) +E (100). All the treatments were given daily for 28 days. Combination of lead and endosulfan modified the indices of oxidative stress in the parameters such as lipid peroxidation, reduced glutathione, superoxide dismutase and catalase in rats as compared to their individual compounds. The results suggest that the combination of these individual compounds may have the potential to modify oxidative stress produced by single compounds in male rats

The Effect of Parathion-methyl and Antidotes on Parotid and Pancreatic Glands: A Pilot Experimental Study

2007 ◽  
Vol 26 (5) ◽  
pp. 383-388 ◽  
Author(s):  
Betul Gulalp ◽  
Yuksel Gokel ◽  
Derya Gumurdulu ◽  
Gulsah Seydaoglu ◽  
Kenan Daglioglu ◽  
...  
Keyword(s):  
Group Iv ◽  
Control Group ◽  
Blood Levels ◽  
Parotid Glands ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Histopathologic Evaluation ◽  
Histologic Changes

The objective of this study is to investigate the functions of parotid and pancreatic glands in response to intoxication with parathion-methyl (PM) and the effects of treatment in rats. Seventy-five male Wistar rats were divided equally into five groups: Group I, control; group II, received atropine and pralidoxime (2-PAM) for 24 h, but no PM; group III, oral PM but no atropine and 2-PAM; group IV, PM and atropine for 24 h and 2-PAM; group V, PM and atropine for 96 h and 2-PAM. After the administration of the chemicals, blood samples were drawn to test for amylase, lipase, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE), while pancreatic and parotid glands of each rat were removed for light microscopic examination. Amylase levels were found significantly elevated in groups II, III, IV, and V, whereas lipase levels were supranormal in groups III, IV, and V. The blood levels of AChE were decreased in groups III and IV and BChE were decreased in II, III, IV, and V. No evidence of pancreatitis and parotitis was identified in the histopathologic evaluation in any group in 96 h; however, hyperchromasia, irregularity in nuclei, and binuclear cells were observed in all parotid glands in group V. Parotitis and pancreatitis were not evident; however, hyperamylasemia and hyperlipasemia were found, whereas various histologic changes in parotid glands were documented in the groups that were administered organophosphate and treatment.

scholarly journals Protective effect of Berberis asiatica root on biochemical and histopathological changes in streptozotocin-induced diabetic Wistar rats

2017 ◽  
Vol 6 (8) ◽  
pp. 1880 ◽  
Author(s):  
Mohd Muddassir Husain Khan ◽  
Chetan Rastogi ◽  
Sachin Gupta ◽  
Shravan Kumar Paswan ◽  
Pritt Verma ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Liver Function ◽  
Blood Glucose Level ◽  
Protective Effect ◽  
Glucose Level ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Group Iv ◽  
Group Iii ◽  
Group I

Background: This study was designed to evaluate the effect of Berberis asiatica root extract (BAE) against streptozotocin induced elevated blood glucose level and other liver and kidney functions changes in adult male Wistar rats.Methods: Thirty male Wistar rats were divided into five groups of six rats in each (Groups I-V). Group I and Group II served as normal control and disease control, respectively. Group III received standard anti-diabetic drug glibenclamide (5mg/kg), while Group IV and Group V received the low dose (250mg/kg) and high dose (500mg/kg) of BAE. Serum blood glucose, SGOT, SGPT, ALP, total bilirubin, BUN, serum creatinine, TC, TG, HDL-C, LDL-C, and VLDL-C were estimated using standard methods. After collection of samples for biochemical evaluation, the pancreas from each animal was isolated and examined for histological changes.Results: BAE and glibenclamide treated disease rats showed significant (p <0.05) decrease in blood glucose concentration. Treatment with BAE at 250mg/kg and 500mg/kg in Group IV and V and standard drug glibenclamide in Group III showed significant (p <0.05) reduction in the level of liver function substances such as SGOT, SGPT, ALP and total bilirubin as compared to disease group, as well as showed significant (p <0.05) decrease in renal panel. Liver function parameters were significantly (p <0.05) improved in groups treated with BAE. Histopathological analysis revealed the protective effect of BAE against streptozotocin induced damage to islets of Langerhans.Conclusions: This study showed the destruction of islets of Langerhans and elevation in blood glucose level as well as alteration in other biochemical parameters were ameliorated by the effect of Berberis asiatica extract.

scholarly journals Effects of Atrazine on Reproductive Health of Nondiabetic and Diabetic Male Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Dinesh Babu Jestadi ◽  
Alugoju Phaniendra ◽  
Undru Babji ◽  
Bhavatharini Shanmuganathan ◽  
Latha Periyasamy
Keyword(s):  
Reproductive System ◽  
Low Dose ◽  
Reproductive Toxicity ◽  
Diabetic Rats ◽  
Group Iv ◽  
Male Rats ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Nonsignificant Decrease

The aim of the present study was to investigate the effects of low dose of atrazine on reproductive system of male Wistar rats. 16 rats were divided into four groups of four animals each. Group I (nondiabetic) and group III (diabetic) animals served as controls that received safflower oil (300 μL/kg bw/day), respectively. Group II (nondiabetic) and group IV (diabetic) animals received atrazine (300 μg/kg bw/day). Nonsignificant decrease in the activities of antioxidant and steroidogenic enzymes and sperm parameters suggests that atrazine did not produce any effect on reproductive system of rats. Histological findings also revealed that atrazine at a dose of 300 μg/kg bw did not produce any testicular toxic effects in nondiabetic and diabetic atrazine treated rats. Low dose of atrazine did not show reproductive toxicity in rats. To know the effects of atrazine in diabetic rats further studies have to be carried out with increased concentration of atrazine.

scholarly journals Quality of Live Improvement Antituberculosis Consumer

2019 ◽  
Vol 2 (1) ◽  
pp. 22-25
Author(s):  
Jumasni Adnan
Keyword(s):  
Lipid Peroxidation ◽  
Liver Damage ◽  
Water Extract ◽  
Group Iv ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Group Ii

Antituberculosis is the most liver damage causes. Rifampicin and Isoniazide, in combination, are toxic compounds. Isoniazide and rifampicin metabolits causes lipid peroxidation. The hepatoprotective effect of rosella calyx water extract on liver damage induced with Isoniazide-rifampicin evaluated by examination of malondialdehid levels in the liver organ. 25 male wistar rats divided into 5 groups, ie group I (INH-rifampicin + rosella water extract 250 mg/kgBW), group II (INH-rifampicin + rosella water extract 125 mg/kgBW), group III (INH-rifampicin + rosella water extract 62.5 mg/kgBW),  group IV (healthy control) and group V (Isoniazide-rifampicin). MDA liver levels were analyzed after 35 days of treatments. The test results of each group are, group I has mean MDA levels 0.023912 + 0.011 mg/ml, group II 0.023526 + 0.009 mg/ml, group III 0.027168 + 0.007 mg/ml group IV 0.03437 + 0.009 mg/ml and group V 0.236846 + 0.118 mg/ml. The kruskal-wallis test showed significantly value 0.008 (p 0.05) and Post hoc Mann U whitney test showed that group V was significantly different to group I, II, III, and IV (p = 0.008) respectively, roselle extract can be used as a hepatoprotector antioxidant to improve the tuberculosis drug consumer quality of life through improved health by lowering lipid peroxidation that causes liver damage.

scholarly journals Synergistic effects of sitagliptin and losartan against fipronil-induced hepatotoxicity in rats

2021 ◽  
pp. 1901-1907
Author(s):  
Sara T. Elazab ◽  
Omar Samir ◽  
Marwa E. Abass
Keyword(s):  
Synergistic Effects ◽  
Study Groups ◽  
Group Iv ◽  
Combination Group ◽  
Tissue Samples ◽  
Once Daily ◽  
Group Iii ◽  
The Past ◽  
Group I ◽  
Male Wistar Rats

Background and Aim: Fipronil (FPN) is a potent pesticide that is heavily used around the world in agriculture. However, its irrational use could potentially have deleterious effects on animals and humans. The present study aimed to investigate the ability of sitagliptin (Sit) and losartan (LOS), when used both individually or concurrently, to guard rat liver against the acute hepatotoxicity caused by FPN. Materials and Methods: Forty-two adult male Wistar rats were equally divided into seven groups (6/group). Group I (control) received normal saline (0.5 mL/rat, vehicle for all treatments) by gavage once daily for 10 days. Group II received oral Sit (10 mg/kg body weight [BW]) daily for 10 days and Group III received oral LOS (5 mg/kg BW) daily for 10 days. Group IV received oral FPN (19.4 mg/kg BW; 1/5 of the oral LD50) for the past 5 days of the study. Groups V and VI received oral Sit (10 mg/kg BW) and LOS (5 mg/kg BW) daily, respectively, 5 days prior and 5 days during FPN administration (19.4 mg/kg BW). Group VII received oral Sit (10 mg/kg BW) and LOS (5 mg/kg BW) for 10 days with daily FPN during the past 5 days. After the end of the treatment period, the rats were humanely sacrificed and blood and liver tissue samples were collected for biochemical analysis and histopathological and immunohistochemical investigations. Results: FPN administration resulted in elevated alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase serum concentrations as well as increased malondialdehyde levels and reduced catalase, superoxide dismutase, glutathione peroxidase, and glutathione activity. The histopathological investigation showed disorganization of the hepatic cords and focal necrosis of the hepatocytes in FPN-intoxicated rats. Furthermore, the immunohistochemical examination showed that hepatic caspase-3 was overexpressed in the FPN-treated rats. The administration of Sit and LOS before and alongside FPN markedly mitigated the alterations caused by FPN and the hepatoprotective effects were more prominent in the combination group. Conclusion: Sit and LOS, both individually or in combination, confers considerable hepatoprotection against FPN-induced hepatotoxicity.

scholarly journals Diosmin Alleviates Doxorubicin-Induced Liver Injury via Modulation of Oxidative Stress-Mediated Hepatic Inflammation and Apoptosis via NfkB and MAPK Pathway: A Preclinical Study

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1998
Author(s):  
Abdullah F. AlAsmari ◽  
Metab Alharbi ◽  
Faleh Alqahtani ◽  
Fawaz Alasmari ◽  
Mohammed AlSwayyed ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Mapk Pathway ◽  
Preclinical Study ◽  
Group Iv ◽  
Control Group ◽  
High Dose ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Pre Treatment

Hepatotoxicity caused by chemotherapeutic drugs (e.g., doxorubicin) is of critical concern in cancer therapy. This study focused on investigating the modulatory effects of diosmin against doxorubicin-induced hepatotoxicity in Male Wistar rats. Male Wistar rats were randomly divided into four groups: Group I was served as control, Group II was treated with doxorubicin (20 mg/kg, intraperitoneal, i.p.), Group III was treated with a combination of doxorubicin and low-dose diosmin (100 mg/kg orally), and Group IV was treated with a combination of doxorubicin and high-dose diosmin (200 mg/kg orally) supplementation. A single dose of doxorubicin (i.p.) caused hepatic impairment, as shown by increases in the concentrations of serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase. Doxorubicin produced histological abnormalities in the liver. In addition, a single injection of doxorubicin increased lipid peroxidation and reduced glutathione, catalase, and superoxide dismutase (SOD) levels. Importantly, pre-treatment with diosmin restored hepatic antioxidant factors and serum enzymatic activities and reduced the inflammatory and apoptotic-mediated proteins and genes. These findings demonstrate that diosmin has a protective effect against doxorubicin-induced hepatotoxicity.

scholarly journals Study of the antineoplastic action of Tabebuia avellanedae in carcinogenesis induced by azoxymethane in mice

2011 ◽  
Vol 26 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Roberta Alves Higa ◽  
Ricardo Dutra Aydos ◽  
Iandara Schettert Silva ◽  
Rondon Tosta Ramalho ◽  
Albert Schiaveto de Souza
Keyword(s):  
Protective Effect ◽  
Colon Carcinogenesis ◽  
Group Iv ◽  
Aberrant Crypt Foci ◽  
Antitumor Action ◽  
Group V ◽  
Group Iii ◽  
Antineoplastic Action ◽  
Group I ◽  
Group Ii

PURPOSE: To study the antitumor action of Tabebuia avellanedae in experimentally induced colon carcinogenesis by azoxymethane in mice. METHODS: Fifty (n=50) mice were divided into five groups: in group I azoxymethane (AOM) was administered, in Group II - β-lapachone, in group III - vehicle (diluent) and in group IV - vehicle + AOM and finally in group V - β-lapachone + AOM. RESULTS: It was observed the presence of aberrant crypt foci in all animals of groups I and IV, 50% in group II and 90% in group V. CONCLUSION: The β-lapachone extracted from the Tabebuia avellanedae showed no protective effect of lesions induced by azoxymethane in colon of mice.

Redox modulating effects of grape juice during aging

2019 ◽  
Vol 31 (3) ◽  
Author(s):  
Raushan Kumar ◽  
Sukanya Bhoumik ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Osmotic Fragility ◽  
Grape Juice ◽  
Redox System ◽  
Group Iv ◽  
Group Iii ◽  
Positive Effects ◽  
Reducing Ability ◽  
Group I ◽  
Male Wistar Rats ◽  
Old Rats

AbstractBackgroundPolyphenols are known because of their phytochemical constituents having antioxidative properties. In this regard, grape juice is highly enriched with polyphenolic constituents, and its supplementation has been known to improve many health and age-associated diseases and risk factors. Our study was entirely dedicated to evaluating the positive effects of grape juice on young and old rats’ erythrocytes and plasma.MethodsYoung (4 months) and old (24 months) male Wistar rats were given an oral dose of grape juice for 28 days. They were grouped into four categories (n = 6): Group I: young control rats; Group II: young grape juice treated rats; Group III: old control rats; Group IV: old treated rats. The treated groups were administered with 10 μL/g of grape juice according to body weight. The following biomarkers of antioxidant defense were measured: ferric reducing ability of plasma (FRAP), reactive oxygen species (ROS), plasma membrane redox system (PMRS), glutathione (GSH), osmotic fragility, and the decrease in lipid peroxidation measured in terms of malondialdehyde (MDA) levels.ResultsA significant increase (p<0.05) in antioxidant levels of FRAP, PMRS, and GSH and a significant decrease (p<0.05) in oxidized products such as ROS and MDA were seen in the treated rats in comparison to the controls. The decrease in ROS and rise in FRAP and PMRS levels suggest the ability of grape juice to combat oxidative stress effectively.ConclusionWe propose the role of grape juice as a potent antioxidant because of its easy bioavailability and its role in combating stress. Our results also approve grape juice as a possible antiaging agent.

scholarly journals Evaluation of concurrent exposure of lower concentrations of lead and endosulfan on apoptosis by scatter pattern of flow cytometry

2021 ◽  
Vol 10 (5) ◽  
pp. 294-297
Author(s):  
V Ranganathan ◽  
◽  
Jitendra Kumar Malik ◽  
GS Rao ◽  
◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Group Iv ◽  
Control Group ◽  
Untreated Control Group ◽  
Technical Grade ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats

The effect of repeated exposure of lower doses of lead and endosulfan were evaluated on apoptosis in male wistar rats. Rats of group I served as untreated control. Group II received drinking water with lead as lead acetate @100 ppm (Pb100). Group III was given feed containing technical grade endosulfan @ 10 ppm (E10). Group IV was exposed to Pb (100) +E (10). Splenocytes were analysed for estimating apoptotic percentage in rats. The results suggest that apoptotic percentage was not changed in the lower doses of endosulfan and lead when administered alone and also in combination in the present study

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