scholarly journals A search for RFLP markers to identify genes for aluminum tolerance in maize

1997 ◽  
Vol 20 (3) ◽  
pp. 459-465 ◽  
Author(s):  
Giovana A. Torres ◽  
Sidney N. Parentoni ◽  
Maurício A. Lopes ◽  
Edilson Paiva
Keyword(s):  
Nutrient Solution ◽  
Inbred Line ◽  
Bulked Segregant Analysis ◽  
Block Design ◽  
Aluminum Tolerance ◽  
Genetic Material ◽  
Chromosome 8 ◽  
Rflp Markers ◽  
Al Tolerance ◽  
F2 Population

The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregant analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotypic measure of tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein referred as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aluminum tolerance on chromosome 8

scholarly journals Aluminum tolerance in castor bean lines1

2018 ◽  
Vol 48 (3) ◽  
pp. 299-305
Author(s):  
Lucas Barbosa de Freitas ◽  
Dirceu Maximino Fernandes ◽  
Suelen Cristina Mendonça Maia ◽  
Laerte Gustavo Pivetta ◽  
Maurício Dutra Zanotto
Keyword(s):  
Castor Bean ◽  
Block Design ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Al Tolerance ◽  
Tropical Regions ◽  
Randomized Block Design ◽  
Bean Plants ◽  
Management Techniques ◽  
Adequate Management

ABSTRACT Castor bean plants are susceptible to aluminum (Al) in the soil, requiring adequate management techniques for their cultivation in acid soils containing high Al levels, as it occurs in tropical regions. This study aimed to assess the Al tolerance of castor bean lines. A randomized block design, in a 2 x 9 factorial scheme, with four replicates, was used. The treatments consisted of presence and absence of Al, as well as nine castor bean lines (CRZ H06, CRZ H11, CRZ H12, CRZ H15, CRZ H17, CRZ H18, CRZ H19, CRZ H22 and FCA). Based on a distribution into quartiles, the lines were divided into two groups. The Al-tolerant group contained the CRZ H06, H11 and H17 lines, while the group susceptible to Al was composed of CRZ H12, H15, H18, H19, H22 and FCA. The FCA and CRZ H17 lines showed the highest growth, when cultivated without Al.

scholarly journals Genetic analysis of aluminum tolerance in Brazilian barleys

2002 ◽  
Vol 37 (8) ◽  
pp. 1099-1103 ◽  
Author(s):  
Euclydes Minella ◽  
Mark Earl Sorrells
Keyword(s):  
Genetic Analysis ◽  
Population Size ◽  
Nutrient Solution ◽  
Single Gene ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Al Toxicity ◽  
Root Tip ◽  
Al Tolerance ◽  
Hematoxylin Staining

Aluminum (Al) toxicity is a major factor limiting barley growth in acid soils, and genotypes with adequate level of tolerance are needed for improving barley adaptation in Brazil. To study the inheritance of Al tolerance in Brazilian barleys, cultivars Antarctica 1, BR 1 and FM 404 were crossed to sensitive Kearney and PFC 8026, and intercrossed. Parental, F1, F2 and F6 generations were grown in nutrient solution containing 0.03, 0.05 and 0.07 mM of Al and classified for tolerance by the root tip hematoxylin staining assay. Tolerant by sensitive F2 progenies segregated three tolerant to one sensitive, fitting the 3:1 ratio expected for a single gene. The F6 populations segregated one tolerant to one sensitive also fitting a monogenic ratio. The F2 seedlings from crosses among tolerant genotypes scored the same as the parents. Since the population size used would allow detection of recombination as low as 7%, the complete absence of Al sensitive recombinants suggests that tolerance in these cultivars is most probably, controlled by the same gene. Thus, the potential for improving Al tolerance through recombination of these genotypes is very low and different gene sources should be evaluated.

Two genes control aluminum tolerance in maize: Genetic and molecular mapping analyses

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 475-482 ◽  
Author(s):  
S T Sibov ◽  
M Gaspar ◽  
M J Silva ◽  
LMM Ottoboni ◽  
P Arruda ◽  
...  
Keyword(s):  
Major Part ◽  
Fragment Length Polymorphism ◽  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Aluminum Tolerance ◽  
Linkage Groups ◽  
Al Tolerance ◽  
Maize Genetic ◽  
Segregating Population ◽  
Restriction Fragment Length

We have identified two loci linked to aluminum (Al) tolerance in the maize inbred line Cat-100-6 by means of restriction fragment length polymorphism (RFLP) and bulked segregant analysis (BSA). A segregating population F2 was obtained from a cross between Cat-100-6 (Al tolerant) × S1587-17 (Al sensitive) parents. Subsequently two DNA bulks of individuals, displaying a contrasting Al tolerance trait were generated from F2. From a total of 158 markers used, 30 markers were identified showing polymorphism between parents and bulks. The segregation results derived from the hybridization from these 30 markers and 56 individuals from F2 revealed 10 markers cosegregating with the Al tolerance which were located in two linkage groups. The linkage groups were composed of 6 and 4 markers, and they were mapped on the short arm of chromosomes 6 and 10, respectively. From these observations, we deduce that two loci are involved in this trait in Cat-100-6 line. QGENE software was used to study the correlation between these two loci and the trait for aluminum tolerance. The results indicate that the locus on chromosome 10 has the stronger effect, and it is responsible for the major part of the variability of the trait.Key words: maize, aluminum tolerance, molecular mapping, somaclonal variation.

scholarly journals Short Communication: Correlation, path analysis, and heritability of phenotypic characters of bread wheat F2 populations

2018 ◽  
Vol 19 (6) ◽  
pp. 2344-2352
Author(s):  
YUDIWANTI WAHYU ◽  
NURWANITA EKASARI PUTRI ◽  
TRI KOESOEMA NINGTYAS ◽  
SURJONO HADI SUTJAHJO ◽  
AMIN NURa
Keyword(s):  
Path Analysis ◽  
Bread Wheat ◽  
Short Communication ◽  
Block Design ◽  
Genetic Material ◽  
High Yield ◽  
Wheat Varieties ◽  
Phenotypic Characters ◽  
F2 Population ◽  
The Relationship

Wahyu Y, Putri NE, Trikoesoemaningtyas, Sutjahjo SH, Nur A. 2018. Short Communication: Correlation, path analysis, and heritability of phenotypic characters of bread wheat F2 populations. Biodiversitas 19: 2344-2352. Wheat varieties are still limited for Indonesia. A high yield wheat plant is necessary to be assembled by selecting plants from a segregated population. The selection will need information on some characters that contribute to the high yield and the relationship among the observed characters. This study aimed to determine the relationship between characters and the effects of the observed characters to grain yield per plant. This research was conducted from July 2017 to March 2018 at Balithi Experimental Farm (1100 m asl.), Cipanas, Cianjur District, West Java, Indonesia. The genetic material used were three F2 population of bread wheat (G1/Se, HP/Se, and Ja/Se) and four parent genotypes. Observations were made on each individual of F2 populations. Parent genotypes were planted by using completely randomized block design with three replications. The results showed that the number of a grain of non-main spike and the number of grain per plant characters had a high correlation in each F2 population and these characters also had positive and significant direct effect to grain weight per plant. It could be a character selection for high yield breeding program. The broad sense heritability was medium to a high value for all characters in F2 G1/Se and HP/Se populations.

scholarly journals QTL Mapping for Gummy Stem Blight Resistance in Watermelon (Citrullus spp.)

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 500
Author(s):  
Eun Su Lee ◽  
Do-Sun Kim ◽  
Sang Gyu Kim ◽  
Yun-Chan Huh ◽  
Chang-Gi Back ◽  
...  
Keyword(s):  
Chromosome 8 ◽  
Chromosome 6 ◽  
Potential Candidate ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
F2 Population ◽  
Leaf Lesion ◽  
Phenotypic Variations ◽  
A Minor ◽  
Selection Of

Watermelon (Citrulluslanatus) is an economically important fruit crop worldwide. Gummy stem blight (GSB) is one of the most damaging diseases encountered during watermelon cultivation. In the present study, we identified quantitative trait loci (QTLs) associated with GSB resistance in an F2 population derived from a cross between maternal-susceptible line ‘920533’ (C. lanatus) and the paternal-resistant line ‘PI 189225’ (C. amarus). The resistance of 178 F2 plants was assessed by two different evaluation methods, including leaf lesion (LL) and stem blight (SB). To analyze the QTLs associated with GSB resistance, a linkage map was constructed covering a total genetic distance of 1070.2 cM. QTL analysis detected three QTLs associated with GSB resistance on chromosome 8 and 6. Among them, two QTLs, qLL8.1 and qSB8.1 on chromosome 8 identified as major QTLs, explaining 10.5 and 10.0% of the phenotypic variations localizing at same area and sharing the same top markers for both LL and SB traits, respectively. A minor QTL, qSB6.1, explains 9.7% of phenotypic variations detected on chromosome 6 only for the SB trait. High-throughput markers were developed and validated for the selection of resistant QTLs using watermelon accessions, and commercial cultivars. Four potential candidate genes were predicted associated with GSB resistance based on the physical location of flanking markers on chromosome 8. These findings will be helpful for the development of watermelon cultivars resistant to GSB.

Quantitative trait loci mapping for biomass yield traits in a Lolium inbred line derived F2 population

Euphytica ◽  
2009 ◽  
Vol 170 (1-2) ◽  
pp. 99-107 ◽  
Author(s):  
U. C. M. Anhalt ◽  
J. S. Heslop-Harrison (Pat) ◽  
H. P. Piepho ◽  
S. Byrne ◽  
S. Barth
Keyword(s):  
Quantitative Trait Loci ◽  
Inbred Line ◽  
Quantitative Trait ◽  
Biomass Yield ◽  
Yield Traits ◽  
Quantitative Trait Loci Mapping ◽  
F2 Population ◽  
Trait Loci

scholarly journals Sweet pepper production in substrate in response to salinity, nutrient solution management and training system

2011 ◽  
Vol 29 (3) ◽  
pp. 275-281 ◽  
Author(s):  
José S Rubio ◽  
Walter E Pereira ◽  
Francisco Garcia-Sanchez ◽  
Luis Murillo ◽  
Antonio L García ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Fruit Quality ◽  
Block Design ◽  
Sweet Pepper ◽  
Training System ◽  
Fruit Yield ◽  
Salt Treatment ◽  
Hoagland Solution ◽  
Half Strength ◽  
Marketable Fruit

The objective of the present study was to evaluate the marketable fruit yield of sweet pepper plants (Capsicum annuum cv. Orlando) in function of the management of nutrient solution with training system. Plants were grown on coconut coir dust under greenhouse conditions in the southeast of Spain. A randomized block design in split-split plot with four blocks was used to test the effect of the nutrient solution strength (full or half-strength Hoagland nutrient solution), training system (two and three stems per plant) and water salinity (saline and non-saline) on total and marketable yield, fruit quality, and fruit mineral concentration. Salt treatment decreased fruit yield by decreasing the fruit fresh weight but not the number of fruits per plant. Under saline and non-saline conditions, the higher yield of fruits was obtained in plants watered with half-strength Hoagland solution, and grown with three stems per plant. Blossom end rot incidence increased under saline conditions or using full-strength Hoagland solution, but decreased with the combination of half-strength Hoagland solution and three-stem training system. Salt treatment also decreased fruit quality in all the treatments due to a decrease in PO2-, SO4(2-), Fe2+;3+, Cu1+;2+ and Mn2+ concentrations, and fruit shape index. Likewise, plants exposed to salinity and watered with half-strength Hoagland solution and trained with three stems showed a reduction in juice glucose and fructose concentration. Based on these results, an increase of the marketable fruit yield could be obtained under non or moderate saline conditions with the implementation of suitable culture practices.

Molecular mapping of a thermosensitive genetic male sterility gene in rice using bulked segregant analysis

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 188-194 ◽  
Author(s):  
P. K. Subudhi ◽  
R. P. Borkakati ◽  
S. S. Virmani ◽  
N. Huang
Keyword(s):  
Male Sterility ◽  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Pcr Primers ◽  
Rflp Markers ◽  
Arbitrary Primers ◽  
Repulsion Phase ◽  
Genetic Male Sterility ◽  
True Breeding ◽  
Efficient Alternative

The thermosensitive genetic male sterility (TGMS) system is considered to be a more efficient alternative to the cytoplasmic male sterility (CMS) system for hybrid rice. An F2 population from a cross between a TGMS mutant line (IR32364TGMS) and IR68 was used to map the TGMS gene tms3(t). Fertile and sterile bulks were constructed following the classification of F2 plants into true breeding sterile, fertile, and segregating fertile plants based on F3 family studies. From the survey of 389 arbitrary primers in bulked segregant analysis, four RAPD markers were identified in which three, OPF182600, OPB19750, and OPAA7550, were linked to tms3(t) in repulsion phase and one, OPAC3640, was linked to tms3(t) in coupling phase. The tms3(t) gene was flanked by OPF182600 and OPAC3640 on one side and by OPAA7550 and OPB19750 on the other side. All four markers were low-copy sequences and two of them (OPF182600 and OPAC3640) detected polymorphism when the markers were used to probe the genomic blots. Subsequently, OPAC3640 was mapped to the short arm of chromosome 6 using a mapping population available at IRRI. However, no RFLP markers from this region showed linkage to tms3(t) owing to the lack of polymorphism between the parents. All RAPD fragments were cloned and partially sequenced from both ends. Thus, PCR primers can be designed to develop PCR markers for marker-assisted breeding to facilitate the transfer of tms3(t) from one genetic background to another.Key words: bulked segregant analysis, gene tagging, marker-assisted selection, RAPD, TGMS.

scholarly journals Characterization of the effects of macronutrient deficiencies in mangabeira seedlings

2012 ◽  
Vol 34 (4) ◽  
pp. 1235-1244 ◽  
Author(s):  
Layara Alexandre Bessa ◽  
Fabiano Guimarães Silva ◽  
Marialva Alvarenga Moreira ◽  
João Paulo Ribeiro Teodoro ◽  
Frederico Antônio Loureiro Soares
Keyword(s):  
Nutrient Solution ◽  
Dry Matter ◽  
Developmental Stages ◽  
Block Design ◽  
Morphological Alterations ◽  
Federal Institute ◽  
Random Block ◽  
Random Block Design ◽  
The Individual ◽  
Hancornia Speciosa

Knowledge of the mineral nutrition requirements of mangabeira (Hancornia speciosa Gomes) is relatively scarce and rudimentary because there is a lack of consistent data concerning its nutritional demands at different developmental stages. The aim of this research was to characterize the visual symptoms of macronutrient deficiencies and to evaluate the effects of these deficiencies on the growth, the production of dry matter, and the leaf content of mangabeira. To achieve this goal, a greenhouse experiment was conducted at the Goiano Federal Institute (Instituto Federal Goiano) in Rio Verde - GO, from January to June 2011 in which mangabeira plants were arranged in a random block design and grown in nutrient solutions. This experiment was replicated four times. The plants were treated with either a complete nutrient solution or a nutrient solution from which the individual macronutrient of interest (nitrogen (N), phosphorous (P), potassium (K), magnesium (Mg), calcium (Ca), or sulfur (S) had been omitted. The omission of a macronutrient from the nutrient solution resulted in morphological alterations that were characteristic symptoms of the particular nutritional deficiency and caused decreases in growth and dry matter mass production. The accumulation of macronutrients displayed the following order in mangabeira leaves: N>K>Ca>P>S>Mg.

scholarly journals Assessment of Genetic Diversity of Promising Castor Been (Ricinus communis L.) Genotypes in Nigeria

2019 ◽  
Vol 11 (3) ◽  
pp. 467-474
Author(s):  
Bolaji Zuluqurineen SALIHU ◽  
Olamide Ahmed FALUSI ◽  
Adeyinka Olufemi ADEPOJU ◽  
Ibrahim Wasiu AROLU ◽  
Oladipupo Yusuf DAUDU ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Gene Diversity ◽  
Ricinus Communis ◽  
Polymorphic Information Content ◽  
Block Design ◽  
Genetic Material ◽  
Morphological Data ◽  
Research Attention ◽  
Ricinus Communis L

Castor oil plant (Ricinus communis L.) is an important oil crop with little research attention in Nigeria. In the present research, extent of genetic diversity among 20 Nigerian castor genotypes was determined using morphological descriptors and molecular markers. The genotypes were laid out on a randomized complete block design with three replicated plots. Molecular genotyping of the genotypes was carried out using genomic Simple Sequence Repeats (SSR). The genotypes revealed high divergence in seed colour, seed shape, seed mottle, seed caruncle and seed sizes. Seedling establishment varied from 70.18% (in Acc. 006) to 93.25% (Acc. 001) with average mean of 81.53%. Raceme length ranged from 15.90 cm to 29.54 cm with population mean of 20.80 cm. The highest seed yield (1222.98 kg/ha) was recorded in Acc. 001 and the least (611.46 kg/ha) was observed in Acc. 006. Seed oil content varied between 32.15% in Acc. 042 and 54.03% in Acc. 006. Agglomerative cluster dendrogram constructed from morphological data showed random distribution of the genotypes into three cluster groups irrespective of the sources/collection points. The genetic diversity based on SSR Marker Analysis revealed high average expected heterozygosity (0.74), Polymorphic information content (0.68), Nei’s gene diversity index (0.72) and Shannon's Information index (1.43). The dendrogram constructed from molecular data grouped the twenty genotypes into three groups at coefficient of 0.34. From these findings, it showed that the twenty genotypes evaluated are divergent in nature and they could serve as good genetic material for castor breeding in Nigeria.

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