scholarly journals Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops

2018 ◽  
Vol 36 (4) ◽  
pp. 473-479
Author(s):  
Aline F Barros ◽  
Vicente P Campos ◽  
Larissa N Souza ◽  
Sarah S Costa ◽  
Willian C Terra ◽  
...  

ABSTRACT Species of the genus Meloidogyne are limiting factors in vegetable crop production. Studies in Brazil about the occurrence of root-knot nematodes in areas of vegetable crop growth have been conducted without using advanced techniques. Using modern techniques, such as biochemical and molecular methods, improves the accuracy of Meloidogyne species identification. The present study characterized species of Meloidogyne in 36 samples associated with vegetable crops using isoenzyme electrophoresis, SCAR markers, and morphological markers, in addition to validating SCAR markers for accurate species identification. The species M. incognita, M. javanica, M. hapla, M. morocciensis, and M. arenaria were identified, with the first two being the most frequent. Here, the species M. arenaria parasitizing scarlet eggplant and M. morocciensis parasitizing pumpkin and cabbage are reported in Brazil for the first time. Esterase electrophoresis efficiently separated the species of Meloidogyne found in vegetable crops; however, SCAR markers were only effective for the identification of M. incognita, M. javanica, and M. hapla, since the primer pair Far/Rar yielded no amplification product to confirm the identity of M. arenaria. The species M.arenaria and M. morocciensis could not be distinguished by the female perineal patterns. Based on the present results, new primers should be designed for the identification of M. arenaria and M. morocciensis.

1998 ◽  
Vol 55 (spe) ◽  
pp. 116-120 ◽  
Author(s):  
E. M. Grassbaugh ◽  
M. A. Bennett

Several factors can influence stand establishment in vegetable crop production. Environmental conditions such as soil physical characteristics, temperature and moisture, various cultural practices, and diseases may all be limiting factors in establishing maximum stands and achieving the highest possible yields. Measures taken to increase stands include soil improvements, implementing cultural practices, and use of chemical and biological seed treatments. Combining seed treatments and cultural/tillage practices to minimize environmental constraints can lead to maximum stands and yields in the production of high quality vegetable crops.


2021 ◽  
Vol 22 (22) ◽  
pp. 12245
Author(s):  
Manoj Kumar ◽  
Ved Prakash Giri ◽  
Shipra Pandey ◽  
Anmol Gupta ◽  
Manish Kumar Patel ◽  
...  

Vegetable cultivation is a promising economic activity, and vegetable consumption is important for human health due to the high nutritional content of vegetables. Vegetables are rich in vitamins, minerals, dietary fiber, and several phytochemical compounds. However, the production of vegetables is insufficient to meet the demand of the ever-increasing population. Plant-growth-promoting rhizobacteria (PGPR) facilitate the growth and production of vegetable crops by acquiring nutrients, producing phytohormones, and protecting them from various detrimental effects. In this review, we highlight well-developed and cutting-edge findings focusing on the role of a PGPR-based bioinoculant formulation in enhancing vegetable crop production. We also discuss the role of PGPR in promoting vegetable crop growth and resisting the adverse effects arising from various abiotic (drought, salinity, heat, heavy metals) and biotic (fungi, bacteria, nematodes, and insect pests) stresses.


Author(s):  
Lana dos Santos ◽  
Marcos Arenales ◽  
Alysson Costa ◽  
Ricardo Santos

This chapter is concerned with a set of optimization problems associated to crop rotation scheduling in the context of vegetable crop production according to some ecological criteria: no crop of the same botanic family is planted in sequence, green manure and fallow periods must be present in any schedule. A core mathematical model called the crop rotation scheduling model is proposed to represent these ecological criteria together with specific technical constraints associated to the growing of vegetable crops. Three optimization problems based on crop rotation schedules are written in detail in this chapter. For each problem, the authors present a general modeling framework and a solution methodology based on a technique known as column generation, which iteratively builds crop rotation plans for a number of plots. Some extensions are also presented, with the aim of incorporating additional characteristics found in production field conditions. This chapter ends with a brief discussion on a set of computational experiments and some suggestions for future research.


2011 ◽  
pp. 236-267
Author(s):  
Lana dos Santos ◽  
Marcos Arenales ◽  
Alysson Costa ◽  
Ricardo Santos

This chapter is concerned with a set of optimization problems associated to crop rotation scheduling in the context of vegetable crop production according to some ecological criteria: no crop of the same botanic family is planted in sequence, green manure and fallow periods must be present in any schedule. A core mathematical model called the crop rotation scheduling model is proposed to represent these ecological criteria together with specific technical constraints associated to the growing of vegetable crops. Three optimization problems based on crop rotation schedules are written in detail in this chapter. For each problem, the authors present a general modeling framework and a solution methodology based on a technique known as column generation, which iteratively builds crop rotation plans for a number of plots. Some extensions are also presented, with the aim of incorporating additional characteristics found in production field conditions. This chapter ends with a brief discussion on a set of computational experiments and some suggestions for future research.


Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1491
Author(s):  
Ioannis O. Giannakou ◽  
Stefanos Kamaras

Root-knot nematodes can cause tremendous losses in vegetable crops. Farmers usually rely on synthetic nematicides to protect their crops. Recently, newly released nematicides are giving farmers an alternative in chemical control for nematodes. In the present study, the efficacy of vintage nematicide was compared to that of a relatively new nematicide, fluopyram. The latter was always more effective in substantially lower concentrations than oxamyl. Fluopyram paralyzed more than 80% J2s after 24 h immersion at the concentration of 0.25 μL L−1, while the percentage was increased close to 100% after immersion for 48 and 96 h. Similar levels of dead J2s were observed after immersion of J2s in oxamyl at concentrations higher than 8 μL L−1 (24 and 48 h) or 4 μL L−1 (96 h). An evident decrease of egg differentiation was observed when fluopyram concentration was increased to 8 μL L−1, while no significant decrease in egg differentiation was recorded at any concentration of oxamyl. Egg hatching was decreased at concentrations of fluopyram higher than 4 μL L−1, while no reduction was observed even when the concentration of oxamyl was increased to 64 μL L−1. The efficacy of fluopyram in soil was superior compared to that of oxamyl. For the first time, the systemic action of fluopyram is recorded in trials with tomato plants. On the other hand, compared to oxamyl, fluopyram seems to be more toxic to non-target organisms such as the entomopathogenic nematodes Steinernema feltiae and Heterorhabditis bacteriophora.


2015 ◽  
Vol 4 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Gamini Sahu ◽  
Surendra K. Gautam ◽  
Aditi N. Poddar

Root knot nematodes (Meloidogyne species) are major pests of vegetable crops causing serious losses in quantity and quality of crop yield. This study involves an assessment of their suitable hosts on the basis of variations in their population densities and body sizes in different vegetable crop plants under field conditions. A random survey of root knot nematode infestation in vegetable crops was conducted in 9 villages of Abhanpur block, Chhattisgarh state, Central India, from January 2012 to March 2012.  Diseased plants were identified on the basis of above ground symptoms and soil and root samples collected by digging. Extraction of nematodes was done by Cobb’s sieving and decantation method and Baerman’s funnel technique. Identification was done microscopically by morphological examination of perineal patterns of female nematodes. Body sizes were measured by using an eyepiece/ocular micrometer. Twenty nine percent of the total farm area surveyed suffered from root knot nematode attack. Among the several genera of vegetable crop plants surveyed, Lycopersicon esculentum, Dolichos lablab, Solanum melongena, Momordica charantia, Daucus carota,Capsicum annum,Cucumis sativus had root galls. Three species of root knot nematodes Meloidogyne incognita, M. javanica, M. areneria were identified from the above hosts and a comparative morphometric analysis of the body, head and neck size ratios of females were done. Non-significant body and head size variations existing between the females from D. lablab, S. melongena, C. annum, D. carota, L. esculentum showed that all the crops are equally susceptible to root knot nematode attack.  However, on the basis of nematode population density, D. carrota appears to be the best suitable host of the Meloidogyne species other than L. esculentum and S. melongena.


EDIS ◽  
2022 ◽  
Vol 2021 (6) ◽  
Author(s):  
Rao Mylavarapu ◽  
George Hochmuth ◽  
Guodong Liu

This publication presents the fertilization recommendations for vegetable crops based on soil tests performed by the UF/IFAS Extension Soil Testing Laboratory (ESTL). It contains the basic information from which ESTL soil test reports and fertilization recommendations are generated. The audiences for this information include commercial and small farmers, crop advisers and consultants, state and local agencies, fertilizer industry, and any interested individuals interested in sustainable nutrient and environmental management. Major revision by Rao Mylavarapu, George Hochmuth, and Guodong Liu; 12 pp. https://edis.ifas.ufl.edu/cv002


Author(s):  
Muhamad Darmawan ◽  
Neviaty Putri Zamani ◽  
Hari Eko Irianto ◽  
Hawis H Madduppa

The green seaweed Caulerpa racemosa is  a seaweed  of  high prospect that is being given emphasis by the Indonesian government. However, C. racemosa in Indonesia may include multiple species level-entity exhibiting morphological overlap and require molecular analysis for species identification. Molecular documentation of species richness of indigenous populations of C. racemosa is essential to underpin cultivar development and conservation of the species to avoid overharvesting. The present study aimed to determine the genetic diversity of C. racemosa and document the haplotype network of the specimens from four different locations (Bintan Island, Jepara, Takalar and Osi Island) using the chloroplast tufA gene. Twenty individuals from four areas were collected and amplified with the chloroplast-encoded gene tufA for species identification against publicly available data. The identification of C. racemosa based on the tufA gene showed that the species found in four locations were C. cylindracea (previously C. racemosa var. cylindracea), C. macra (previously C. racemosa var. macra), C. racemosa, and C. oligophylla (previously C. racemosa var. lamourouxii). This study records the existence of C. cylindracea in Takalar and Jepara, Indonesia for the first time. The most diverse C. racemosa species was in Osi Island, where the exploitation of this seaweed is very low. In contrast, the lowest number of C. racemosa varieties were found in Takalar, where exploitation is very high. There were only minor light variations of Caulerpa species in the tufA gene in four different sites with only four haplotypes found, and each haplotype corresponded to another species. 


Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1569
Author(s):  
Spyridon A. Petropoulos

The research interest on plant biostimulant applications in vegetable crop production is gradually increasing and several reports highlight the beneficial effects that such products may have not only on crop performance but also on the quality of the final product. Moreover, numerous products with biostimulatory activity are being developed which need further evaluation under variable growing conditions and different crops. Plant hydrolysates which contain amino acids and peptides have been acclaimed with several positive effects on crop performance of diverse horticultural crops, while macro-algae are also considered effective biostimulants on plants grown under stress conditions. A recent study evaluated the use of protein hydrolysates and brown macro-algae (Ascophyllum nodosum and Ecklonia maxima) as innovative and cost effective approaches for sustainable vegetable production. The present editorial provides an overview of the main findings of that study, while discussing the practical applications that biostimulants may have in the greenhouse production of vegetable crops, aiming to increase the yield and the quality of the final produce and improve crop tolerance to abiotic stressors.


EDIS ◽  
2017 ◽  
Vol 2017 (6) ◽  
Author(s):  
Rao S. Mylavarapu ◽  
George J. Hochmuth ◽  
Guodong Liu

This publication presents the fertilization recommendations for vegetable crops based on soil tests performed by the IFAS Extension Soil Testing Laboratory (ESTL). It contains the basic information from which ESTL soil-test reports and fertilization recommendations are generated. Additional information on nutrient recommendations is presented in the Vegetable Production Handbook of Florida, 2017-2018. Similarly, IFAS Standardized Nutrient Recommendations for Agronomic Crops can be found in SL129 (Mylavarapu, 2015).  


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