scholarly journals Action of tacrolimus on Wistar rat kidneys implanted with Walker 256 carcinosarcoma

2010 ◽  
Vol 25 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Cristiano Machado Inácio ◽  
Ulrich Andréas Dietz ◽  
Osvaldo Malafaia ◽  
Jurandir Marcondes Ribas Filho ◽  
Paulo Afonso Nunes Nassif ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Statistical Significance ◽  
Control Group ◽  
Walker 256 Carcinosarcoma ◽  
Tumor Group ◽  
Male Wistar Rats ◽  
Walker 256 ◽  
The Right

PURPOSE: To evaluate the development of Walker 256 tumor in male Wistar rats treated with tacrolimus using an experimental kidney tumor model. METHODS: 40 male Wistar rats were divided into four groups: Tumor group (TU) (n=10), Tacrolimus-Tumor group (TT) (n=10), Tacrolimus group (TC) (n=10) and Control group (C) (n=10). Treatment with tacrolimus was performed in groups TT and TC. Under anesthesia, the right kidney of each animal of TU and TT was accessed through a supraumbilical incision and inoculated with a 0.1mL solution containing 2x10(6) tumor cells (Walker 256 carcinosarcoma tumor cells). Group TC was treated with a saline solution. All the animals of groups TC and TT were treated with tacrolimus (5mg/kg/day) by gavage for 15 days. TU group animals received saline by gavage for 15 days. On the 15th postoperative day, all animals were submitted to euthanasia and blood sampling for analysis of serum creatinine (Cr) and blood urea nitrogen (BUN). Abdominal gross examination was performed, the right kidney removed and prepared for histological analysis by hematoxylin-eosin staining. The resulting data were submitted to statistical analysis by ANOVA. RESULTS: Statistical significance was found when comparing creatinine level between groups TU, TT and TC -TT group culminated with a marked increased in creatinine levels (Cr=1.013 ± 0.3028 mg/mL), TU group (Cr=0.5670 ± 0.03536 mg/dL) P=0.00256, TC group (Cr =0.711 ± 0.1653 mg/mL) P= 0.02832. Statistical significance was found when comparing BUN levels in TT group (71.32 ± 17.14 mg/mL), compared with TU group (45.83 ± 5.046 mg/dL), P=0.000318. There were no statistically significant differences between groups TT and TC (61.23 ± 9.503 mg/mL) P=0.7242. Histological analysis showed a poor evolution in TT group with multiple foci of hemorrhage and cortical invasion by the Walker tumor. CONCLUSION: The Tacrolimus-treated group developed a more aggressive tumor and a drug-related nephrotoxic effect.

scholarly journals Morphological alterations in the heart and aorta of rats treated with glucocorticoids

2015 ◽  
Vol 32 (04) ◽  
pp. 231-235
Author(s):  
R. Dantas ◽  
K. Souza ◽  
D. Santos ◽  
V. Feitosa ◽  
E. Fioretto ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Histological Analysis ◽  
Saline Solution ◽  
Morphological Changes ◽  
Morphological Structure ◽  
Control Group ◽  
Morphological Alterations ◽  
Heart Chamber ◽  
Male Wistar Rats ◽  
Synthetic Glucocorticoid

Abstract Introduction: The objective of this study was to analyze the morphological structure of the heart and aorta of rats treated with the synthetic glucocorticoid dexamethasone. Material and Methods: Male Wistar rats were divided into two groups: 08 control rats undergoing treatment with a 0.9% saline solution for 10 days and 08 rats treated for 10 days with dexamethasone (2mg/kg animal weight). Results: Histological analysis detected a mild cardiac hypertrophy and 15% reduction of collagen located in the aorta of animals treated with glucocorticoid when compared to the control group. Conclusion: We conclude that treatment with dexamethasone for a period of 10 consecutive days is able to promote morphological changes in the structure of the heart chamber and, impair morphological structure of aorta.

scholarly journals The effect of X-ray irradiation to the formation of polychromatic erythrocyte cell micronucleus in Wistar rats (Rattus norvegicus)

2021 ◽  
Vol 54 (3) ◽  
pp. 128
Author(s):  
Eha Renwi Astuti ◽  
Hutojo Djajakusuma ◽  
Indeswati Diyatri ◽  
Nastiti Faradilla Ramadhani
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Treatment Plan ◽  
Statistical Significance ◽  
Clinical Parameter ◽  
Control Group ◽  
X Ray ◽  
Polychromatic Erythrocytes ◽  
Male Wistar Rats ◽  
Group 2

Background: Panoramic and cephalometric radiography is very important for diagnosis, treatment plan, and evaluation of orthodontic treatment results. Panoramic and cephalometric radiography are frequently performed at the same time, causing DNA damage and chromosome aberration. Purpose: This study aims to analyse the effect of X-ray exposure in panoramic and cephalometric radiography on micronuclei cell numbers. Methods: Laboratory-based analytical study with 60 healthy-male Wistar rats weighing 200–300 grams divided into 6 treatment groups (n=10). The control group: without radiographic exposure, the treatment group 2: using panoramic radiographic exposure followed by cephalometric, and the treatment group 3: using panoramic radiographic exposure and 24 hours later performed cephalometric radiographic. The unit of analysis was the polychromatic erythrocytes of mice cell, were examined 24 hours and 48 hours after irradiation had been finished. The polychromatic erythrocytes were examined using May-Gruenwald-Giemsa staining and 100x magnification under a microscope with 2000 cells per view. Data obtained were analysed using the SPSS 20 version software. The mean and standard deviations were calculated for each clinical parameter, and a one‐way ANOVA statistical test of significance was used. Statistical significance was set at p<0.05. Results: The analysis showed a significant increase (p<0.05) in the number of micronucleus in groups that used panoramic radiographic exposure followed by cephalometric. Conclusion: X-ray radiation can increase the number of micronucleus in polychromatic erythrocyte cells in rats.

scholarly journals Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats

2016 ◽  
Vol 60 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Esrafil Mansouri ◽  
Majid Asadi-Samani ◽  
Wesam Kooti ◽  
Maryam Ghasemiboroon ◽  
Damoon Ashtary-Larky ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Sperm Count ◽  
Seed Extract ◽  
Male Rats ◽  
Control Group ◽  
Alcoholic Extract ◽  
Foeniculum Vulgare ◽  
Fertility Effect ◽  
Male Wistar Rats ◽  
The Right

AbstractIntroduction: The study aimed to investigate the anti-fertility effect of fennel (Foeniculim vulgare Mill) seed extract in male rats.Material and Methods: Forty Wistar rats were divided into five equal groups. The control group received distilled water and the experimental groups were orally administered 1 ml of hydro-alcoholic extract of fennel seed in four doses of 35, 70, 140, and 280 mg/kg/b.w. daily for 60 days. After the last gavage, the rats were anaesthetised and the caudal part of the right epididymis was used for sperm counting. After fixation of the testes, microscopic sections were prepared and histological changes were evaluated.Results: The number of spermatogonia after doses of 140 and 280 mg/kg and Sertoli cells after a dose of 140 mg/kg decreased significantly as compared with the control group (P < 0.05). The number of primary spermatocytes and sperm count decreased significantly in the experimental groups (70, 140, and 280 mg/kg) when compared to the control group (P < 0.05). Furthermore, thickening of the basement membrane, cell apoptosis, and irregular arrangement of the germinal epithelium were observed in the experimental groups.Conclusion: Hydro-alcoholic fennel seed extract at these doses could reduce reproductivity and has anti-fertility activity in male rats.

scholarly journals Effects of coffee (Coffea arabica) consumption on the femoral morphology and biomechanics in rats

2014 ◽  
Vol 31 (01) ◽  
pp. 042-047
Author(s):  
M. Santos ◽  
J. Pagani ◽  
T. Silva ◽  
J. Garcia ◽  
M. Romào ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Mechanical Test ◽  
Coffee Consumption ◽  
Control Group ◽  
Bone Properties ◽  
Bone Width ◽  
Femoral Morphology ◽  
Male Wistar Rats ◽  
The Right ◽  
Number Of Individuals

Abstract Introduction: Coffee consumption has proved to be harmful to osseous tissue integrity, thus, diminishing bone resistance. Considering the great number of individuals consuming coffee daily, therefore, the present study aims to demonstrate the effects of coffee on the femoral morphology and biomechanics resistance in Wistar rats. Methods: Twenty male Wistar rats were chosen for the experiment, divided into two groups (n = 10), as to know, CT (control) group, to which was offered water diet ad libitum; and, CF (coffee) group, to which was offered coffee as liquid diet. After 60 days, the animals had their blood analyzed for serum calcium level, they were euthanized and the right femurs were collected for histological procedure and the left ones were isolated for bone measures and mechanical test. Results: CF group animals do not present malnutrition or dehydration. Femur dimensions, trabecular bone width and cortical width analyses shown to be lower for CF group. Material and structural properties of the femurs of CF group animals demonstrate that this group presents weaker bones than those of CT group. Conclusion: The findings in the present study allow us to confirm that coffee consumption interferes negatively on the material and structural bone properties, diminishes trabecular and cortical bone density, and hence making bones more fragile and likely to fractures. So, due to the great number of individuals consuming coffee daily, it is important to reduce this consume on order to prevent bone trauma.

scholarly journals Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats

2021 ◽  
Vol 31 (2) ◽  
pp. 9-13
Author(s):  
E Bachmeier ◽  
◽  
ME Migueles Goitea ◽  
JA Linares ◽  
EG Ferreyra Mariani ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Control Group ◽  
P Value ◽  
Sod Activity ◽  
Paired Samples ◽  
Randomized Design ◽  
Male Wistar Rats ◽  
Student’S T

The objective of the present work was to evaluate the effect of carboplatin (Cp) on submandibular gland homogenate of Wistar rats through the determination of malondialdehyde levels, as the main end product of lipoperoxidation, in an experimental model.Sixteen three-month-old male Wistar rats were used, housed in individual cages, with controlled temperature and lighting and free diet. A completely randomized design was used and two experimental groups were established: 1) Control (C), administering an intraperitoneal dose of saline solution for one day, n: 8, 2) Animals treated with carboplatin (Cp) applying a dose i.p. of 100 mg / Kg of body weight for one day, n: 8. The animals were fasted for 24 hours and subsequently anesthetized. Then both submandibular glands were removed. Malondialdehyde levels were analyzed in submandibular gland homogenatein both experimental groups. Variations between the groups analyzed were evaluated using the Student's t test for paired samples, setting a p-value <0.05 for statistical significance. Project approved by CICUAL. Faculty of Medical Sciences (UNC).The group of Rats Co showed a concentration of 7.32± 0.48μmol / mg of gland. The Cp group had a concentration of 12.57 ± 0.71 μmol / mg of gland, expressing a significant decrease compared to the control group p <0.0006. Cp at the dose tested would cause a decrease in lipoperoxidation in the submandibular gland of rats. Possibly the glandular antioxidant battery would neutralize the oxidative stress of acinar cells. These results suggest a future evaluation of superoxide dismutase (SOD) activity and uric acid (UA) levels

scholarly journals Neurocognitive and Neuroarchitectural Changes in the Prefrontal Cortex of Wistar Rats Treated with Highly Active Antiretroviral Therapy

2021 ◽  
Vol 12 (2) ◽  
pp. 67-75
Author(s):  
Olufunke Dosumu ◽  
◽  
Edidiong Akang ◽  
Edem Edem ◽  
Samuel Afolayan ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Antiretroviral Therapy ◽  
Highly Active Antiretroviral Therapy ◽  
Wistar Rats ◽  
Pyramidal Cells ◽  
Control Group ◽  
Highly Active ◽  
Y Maze ◽  
Male Wistar Rats ◽  
The Right

The use of highly active antiretroviral therapy has proven to be highly effective in the treatment of human immunodeficiency virus 1 (HIV-1) infection. However, its impact on cognition has not been fully explored. This study was designed to assess the impacts of antiretroviral therapy on cognitive function and histoarchitecture of the prefrontal cortex of Wistar rats. Forty adult male Wistar rats weighing 180-200 g were randomly assigned to 4 groups: control, tenofovir, lamivudine and efavirenz (n=10), which received 1 ml distilled water and 6 mg/kg, 6 mg/kg and 12 mg/kg, respectively. Spatial memory scores were assessed using the Y-maze test. Following behavioural studies, the animals were euthanized, and their whole brains harvested. The prefrontal cortex was sectioned and processed for oxidative stress, histological and immunohistochemical analyses. There was a significant decrease in percentage alternation evaluated from the right/wrong decisions scored from the tenofovir and lamivudine groups, compared to the control group (p<0.05). malondialdehyde (MDA) and reduced glutathione (GSH) levels were elevated following lamivudine and tenofovir exposure in the rats’ prefrontal cortices, respectively, compared to control (p<0.05). There were also significant alterations of cortical pyramidal cells in the tenofovir and lamivudine groups. Additionally, marked astrogliosis with increased glial fibrillary acidic protein expression was observed, consistent with the structural alterations, especially in the lamivudine group. Our findings suggest that, of the three highly active antiretroviral therapy (HAART) drugs studied, lamivudine may be a major culprit in the progressive neurological damage and cognitive impairment in HIV-infected individuals on HAART.

scholarly journals Radiographic and histological evaluation of bisphosphonate alendronate and metotrexate effects on rat mandibles inoculated with Walker 256 carcinosarcoma

2007 ◽  
Vol 22 (6) ◽  
pp. 457-464 ◽  
Author(s):  
Ana Paula Negreiros Nunes Alves ◽  
Cláudia do Ó Pessoa ◽  
Letícia Costa-Lotufo ◽  
Manoel Odorico Moraes Filho
Keyword(s):  
Histological Analysis ◽  
Histological Evaluation ◽  
Control Group ◽  
Radiographic Images ◽  
Walker 256 Carcinosarcoma ◽  
Central Regions ◽  
Malignant Lesions ◽  
Walker 256 ◽  
Bone Trabeculae ◽  
Osteoid Formation

PURPOSE: To investigate the effects of bisphosphosnate alendronate (ALD) and metotrexate (MTX) on an experimental model of Walker 256 carcinosarcoma developed in the oral cavity of rats. METHODS Walker 256 carcinosarcoma cell suspension (0,1 mL) containing 10(6) cell/mL was implanted in the alveoli of the first and second molars. The animals were divided and treated with saline, MTX, ALD, and MTX plus ALD. Later, the animals were sacrificed, the tumors were measured and the mandibles were removed for radiographic and histological analysis. RESULTS: In the control group, the radiographic images demonstrated radioluscency with poorly defined borders, and the microscopic examination revealed tumor infiltration into the peripheral and central regions of the bone. Areas of necrosis were commonly seen. In the treated groups with ALD, associated or not with MTX, the radiographic analysis revealed circumscribed tumor-induced osteolysis and various degrees of radiotransparence; while, histologically, preserved bone trabeculae with osteoid formation was observed among malignant cells. CONCLUSION: The bisphosphonate alendronate exherted an osteoprotective effect and induced bone neoformation on the Walker 256 carcinosarcoma inoculated in rat mandibles. The combination of metotrexate with bisphosphonate alendronate is more successful than treatment with the agents alone in controlling the growth of neoplastic cells and in stimulating reactive new bone. Therefore, this may be an alternative treatment to malignant lesions of maxillaries with osteolysis.

scholarly journals Platelet-rich plasma in bone repair of irradiated tibiae of Wistar rats

2010 ◽  
Vol 25 (3) ◽  
pp. 257-263 ◽  
Author(s):  
Emne Hammoud Gumieiro ◽  
Márcio Abrahão ◽  
Ricardo Schmitutz Jahn ◽  
Helena Segretto ◽  
Maria Tereza de Seixas Alves ◽  
...  
Keyword(s):  
Bone Formation ◽  
Wistar Rats ◽  
Bone Repair ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Regeneration Period ◽  
Male Wistar Rats ◽  
Cellular Regeneration ◽  
Histological Processing ◽  
The Right

PURPOSE: To evaluate the influence of PRP addition on bone repair of circular defects created in irradiated tibiae of rats by histometric analysis. METHODS: Sixty male Wistar rats had the right tibiae irradiated with 30 Gy. After 30 days monocortical defects were created and platelet-rich plasma was applied in 30 rats. In the control group defects were created but not filled. The animals were desanguinated after 4, 7, 14, 21, 56 and 84 days and the tibiae removed for histological processing. RESULTS: There was a tendency in the PRP group to increased bone neoformation from 14-days to 84-days; in the control group increased bone neoformation was not seen after 21 days or later. CONCLUSION: The addition of platelet-rich plasma had a beneficial effect in the initial cellular regeneration period and enhanced bone formation in later periods when compared to control.

Correlation of Metabolic Risk Factors with Sessile Serrated Lesions

2020 ◽  
Vol 29 (2) ◽  
pp. 175-179
Author(s):  
Melania Macarie ◽  
Simona Bataga ◽  
Simona Mocan ◽  
Monica Pantea ◽  
Razvan Opaschi ◽  
...  
Keyword(s):  
Risk Factors ◽  
Statistical Significance ◽  
Interval Cancer ◽  
Metabolic Risk Factors ◽  
Control Group ◽  
Anatomical Site ◽  
Metabolic Risk ◽  
Right Colon ◽  
Serrated Lesions ◽  
The Right

Background and Aims: The importance of sessile serrated lesions (SSLs) in the pathogenesis of colorectal carcinoma has been recently established. These are supposed to cause the so-called “interval cancer”, having a rapidly progressive growth and being difficult to detect and to obtain an endoscopic complete resection. We aimed to establish the most important metabolic risk factors for sessile serrated lesions. Methods: We performed a retrospective case-control study, on a series of 2918 consecutive patients who underwent colonoscopy in Gastroenterology and Endoscopy Unit, County Clinical Emergency Hospital, Târgu-Mureș, Romania between 1 st of January 2015-31 th of December 2017. In order to evaluate the metabolic risk factors for polyps’ development, enrolled participants were stratified in two groups, a study group, 33 patients with SSLs lesions, and a control group, 138 patients with adenomatous polyps, selected by systematic sampling for age and anatomical site. Independent variables investigated were: gender, smoking, alcohol consumption, obesity, arterial hypertension, diabetes, hypercholesterolemia, hypertriglyceridemia, hyperuricemia, nonalcoholic liver disease. Results: For SSLs the most common encountered localization was the right colon in 30.55% of cases. By comparative bivariate analysis between SSLs group and control group, it was observed that hypertension (p=0.03, OR 2.33, 95 %CI 1.03-5.24), obesity (p=0.03, OR 2.61, 95 %CI 1.08-6.30), hyperuricemia (p=0.04, OR 2.72, 95 %CI 1.28-7.55), high cholesterol (p=0.002, OR 3.42; 95 %CI 1.48-7.87), and high triglycerides level (p=0.0006, OR 5.75; 95 %CI 1.92-17.2) were statistically associated with SSLs development. By multivariate analysis hypertension and hypertriglyceridemia retained statistical significance. Conclusions: Our study showed that the highest prevalence of SSLs was in the right colon and hypertension and increased triglycerides levels were associated with the risk of SSLs development. These risk factors are easy to detect in clinical practice and may help identifying groups with high risk for colorectal cancer, where screening is recommended.

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

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