Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

scholarly journals Effect of purple sweet potato (Ipomoea batatas L) extract on malondialdehyde levels of male white rat (Rattus norvegicus Wistar strain) model of atherosclerosis

2019 ◽  
Author(s):  
Meddy Setiawan ◽  
Fakih Nadhil
Keyword(s):  
Sweet Potato ◽  
Rattus Norvegicus ◽  
Ipomoea Batatas ◽  
Control Treatment ◽  
Control Group ◽  
Experimental Animals ◽  
Wistar Strain ◽  
Potato Extract ◽  
Purple Sweet Potato ◽  
Strain Model

Cardiovascular disease is a disease caused by impaired functions of heart and blood vessel, one of those is coronary heart disease. This study aimed to identify the effect of purple sweet potato (Ipomoea batatas L) extract on MDA (Malondialdehyde) levels of male white rat (Rattus norvegicus Wistar strain) model of atherosclerosis. This research was an experimental study using Post-Test Only Control Group Design method, used 5 groups (positive control group, negative control, treatment 1, treatment 2, and treatment 3), each group contained 5 rats plus 2 reserve rats. ANOVA one way test obtained a significance of 0.00 (p <0.05). The regression test results showed the coefficient of determination R² = 0.71, which means a decrease in serum MDA levels of experimental animals is influenced by the dose of purple sweet potato extract administration as much as 71% while the remaining 29% can be influenced by factors outside the study. It can be concluded that purple sweet potato extract can affect serum MDA levels of experimental animals.

scholarly journals Gambaran histopatologik ginjal tikus wistar (Rattus norvegicus) yang diinduksi gentamisin dan diberikan ubi jalar ungu (Ipomoea batatas L. Poir)

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Grace S. Siahaan ◽  
Poppy M. Lintong ◽  
Lily L. Loho
Keyword(s):  
Epithelial Cells ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Iv ◽  
Kidney Tubule ◽  
Hydropic Degeneration ◽  
Group I ◽  
Potato Extract ◽  
Purple Sweet Potato

Abstract: Gentamycin is known as one of the nephrotoxic agents. Purple sweet potato (Ipomoea batatas L. Poir) is rich of antioxidant due to its high anthocyanin content. This study aimed to obtain the histopathological features of the kidney of Wistar rats induced by gentamycin and were given purple sweet potato. This was an experimental laboratory study. Subjects were 24 Wistar rats. Group I (negative control) consisted of 4 rats, fed with pellets (AD2) for 7 days. Group IIA and IIB consisted of 4 rats each, induced by gentamicin for 7 and 10 days each. Group IIIA and IIIB consisted of 4 rats each, induced by gentamicin for 7 and 10 days and then were given purple sweetpotato extract for 7 days. Group IV consisted of 4 rats induced by gentamicin for 7 days then were fed with pellets (AD2) for 7 days. The results showed hydropic degeneration, necrosis, and fibrosis of kidney tubule epithelial cells in group IIA and B. Group III A and B showed regeneration of kidney tubule epithelial cells, while hydropic degeneration and necrosis focus reduced more than group II A and B. Group IV showed epithelial cell regeneration, yet still showed hydropic degeneration and necrosis. Conclusion: Supplementation of purple sweet potato extract to Wistar rats induced with gentamycin showed more regeneration of kidney tubule epithelial cells compared to the Wistar rats without supplementation of purple sweet potato extract. Keywords: kidney, gentamicin, purple sweet potato extract Abstrak: Gentamisin merupakan salah satu agen nefrotoksik. Ubijalar ungu (Ipomoea batatas L. Poir) sebagai tanaman kaya antioksidan memiliki senyawa antosianin tinggi. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik ginjal tikus wistar yang diinduksi gentamisin dan diberikan ubi jalar ungu (Ipomoea batatas L. Poir). Jenis penelitian ini eksperimental laboratorik dengan menggunakan 24 ekor tikus wistar sebagai hewan coba. Kelompok I (kontrol negatif) sebanyak 4 ekor hanya diberi makan pelet AD2 selama 7 hari. Kelompok IIA dan IIB masing-masing sebanyak 4 ekor, diinduksi gentamisin selama 7 dan 10 hari. Kelompok IIIA dan IIIB masing-masing sebanyak 4 ekor, diinduksi gentamisin selama 7 dan 10 hari kemudian diberikan ekstrak ubijalar ungu selama 7 hari. Kelompok IV sebanyak 4 ekor diinduksi gentamisin selama 7 hari dan diberikan pelet AD2 selama 7 hari. Hasil penelitian menunjukkan degenerasi hidropik, nekrosis dan fibrosis pada sel epitel tubulus ginjal tikus yang diberi gentamisin. Pemberian ekstrak ubi jalar ungu pada tikus yang diinduksi gentamisin menunjukkan regenerasi sel epitel tubulus ginjal, sedangkan fokus degenerasi hidropik dan nekrosis semakin berkurang. Kelompok tanpa pemberian ekstrak ubi jalar ungu menunjukkan regenerasi sel epitel namun gambaran degenerasi hidropik dan nekrosis masih tampak. Simpulan: Pemberian ekstrak ubi jalar ungu pada tikus wistar paska induksi gentamisin menunjukkan regenerasi sel epitel tubulus lebih luas dibandingkan yang tanpa pemberian ekstrak ubijalar ungu.Kata kunci: ginjal, gentamisin, ekstrak ubijalar ungu

scholarly journals Balinese Cultivar of Purple Sweet Potato Improved Neurological Score and BDNF and Reduced Caspase-Independent Apoptosis among Wistar Rats with Ischemic Stroke

2019 ◽  
Vol 7 (1) ◽  
pp. 38-44 ◽  
Author(s):  
I Made Oka Adnyana ◽  
Raka Sudewi ◽  
Purwa Samatra ◽  
Suprapta Suprapta
Keyword(s):  
Antioxidant Activity ◽  
Ischemic Stroke ◽  
Sweet Potato ◽  
Neuronal Death ◽  
Wistar Rats ◽  
Intrinsic Pathway ◽  
Neurological Score ◽  
Significant Difference ◽  
Potato Extract ◽  
Purple Sweet Potato

BACKGROUND: Ischemic stroke occurs due to the abrupt occlusion in the brain which leads to neuronal death. Neuronal death in ischemic stroke is due to increase production of reactive oxygen species (ROS). Neuronal death occurs via necrosis and apoptosis mechanisms. Apoptosis can either occur via extrinsic or intrinsic pathway. Meanwhile, the intrinsic pathway can be caspase-dependent or independent. Anthocyanin is a natural pigment with antioxidant, anti-inflammatory, anti-cancer, and neuroprotective properties. Balinese cultivate of purple potato extract contains a high level of anthocyanin and has been proven for its antioxidant activity. AIM: Antioxidant effect of Balinese cultivates purple potato extract has not been studied on an animal model with ischemic stroke. Accordingly, we would like to study the effect of antioxidant properties from Balinese cultivate of purple potato extract by assessing the neurological score, BNDF concentration, and caspase-independent apoptosis by measuring AIF concentration on Wistar rats with ischemic stroke. METHODS: This was an experimental study using male Wistar rats age between 12-14 weeks weigh between 200 to 250 g. RESULTS: This study demonstrated a significant difference of neurological score on day 3 among control versus treatment groups. Balinese cultivate of purple potato extract markedly reduced AIF, increased BDNF, and suppressed apoptosis among treatment group when compared with the control group. CONCLUSION: We have proven the efficacy of antioxidant activity of anthocyanin derived from Balinese cultivar of purple sweet potato by elevated AIF levels, lower apoptosis rate, improved neurological score on day-3 to day-7 post-stroke, as well as increased BDNF levels.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals The influence of addition of various sweet potatoes (Ipomea batatas) extract to total count of cells and antioxidant activity in yogurt

2009 ◽  
Vol 7 (2) ◽  
pp. 68-76
Author(s):  
RETNATI RETNATI ◽  
M.A.M. ANDRIANI ◽  
GUSTI FAUZA
Keyword(s):  
Antioxidant Activity ◽  
Sweet Potato ◽  
Skim Milk ◽  
Total Count ◽  
Sweet Potatoes ◽  
Fresh Milk ◽  
Ipomea Batatas ◽  
Significant Difference ◽  
Potato Extract ◽  
Purple Sweet Potato

Retnati, Andriani MAM, Fauza G. 2009. The influence of addition of various sweet potatoes (Ipomea batatas) extract to total count of cells and antioxidant activity in yogurt. Biofarmasi 7: 68-76. The aim of this research was to determine the influence of addition of various sweet potatoes extract to the total count of cells and the antioxidant activity in yogurt. Yogurt was made from fresh milk, skim milk, white sweet potato, orange sweet potato, purple sweet potato, and pure culture of Streptococcus thermophilus 0040 and Lactobacillus bulgaricus 0041 in straight MRS agar. Fresh milk, skim milk powder (5%, b/v), and sweet potato extract (10%, v/v) was pasteurized at 90oC for 15 minutes, cooled to the temperature between 40-45oC, inoculated with 2.5% S. thermophilus and L. bulgaricus with a proportion of 1.4:1, and then incubated at a temperature of 40oC for 15 hours. Yogurt without an addition of sweet potato extract was used as control. The parameters measured in this experiment were the total count of cells with TPC (Total Plate Count) method and the antioxidant activity with DPPH (2,2- diphenyl-1-picrylhydrazyl) method. Samples were taken at one hour interval to examine the total count of cells, while the antioxidant activity was collected at three hours interval. The result of each analysis was plotted into graphics which describing the relation of total bacteria and antioxidant activity with fermentation time. ANOVA was employed to analyze the data. If there was a significant difference, it should be followed by Duncan Multiple Range Test (DMRT) at a significance level α=0.05. The result of this research showed that the addition of various sweet potatoes extracts increased the total count of cells and the antioxidant activity in yogurt. The total count of cells showed no significant different for each sample, it meant that the different colors in sweet potato did not influence the total count of cells. However, yogurt with orange and purple sweet potato extract addition had a significant difference on the antioxidant activity with control and yogurt with white sweet potato extract. In conclusion, the difference colors in sweet potato influenced in the antioxidant activity in yogurt significantly. Sweet potato is potential for milk substitute in yogurt production due to oligosaccharide content and antioxidant activity.

scholarly journals Pengaruh pemberian ubi jalar ungu (Ipomoea batatas L.Poir) terhadap kadar superoksida dismutase (SOD) tikus wistar jantan (Rattus Norvegicus) yang dipapar asap rokok

2020 ◽  
Vol 8 (1) ◽  
pp. 45
Author(s):  
Alfreda Sabrina Widyanti ◽  
Martha Ardiaria ◽  
Nurmasari Widyastuti
Keyword(s):  
Superoxide Dismutase ◽  
Sweet Potato ◽  
Cigarette Smoke ◽  
Ipomoea Batatas ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Standard Diet ◽  
Control Group Design ◽  
Significant Difference

Background: Cigarette smoke exposure is one of the source of free radicals that causes oxidative stress and decreases superoxide dismutase (SOD) level. Purple fleshed sweet potato is a type of food that contains antioxidants to neutralize oxidative stress.Objectives: To study the effect of purple fleshed sweet potato on superoxide dismutase (sod) level on rats exposed to cigarette smoke.Methods: This was a true experimental study with a post-test randomized control group design. The rats were randomized into 4 groups (6 rats in each group). The negative control group (K-) was treated with standard diet; the positive control group (K+) was treated with cigarette smoke exposure and standard diet; the treatment 1 (P1) group was treated with standard diet and purple fleshed sweet potato with the dose of 8 g / 200 g bw/day, and the treatment 2 (P2) group was treated with cigarette smoke exposure and purple fleshed sweet potato with the dose of 8 g /200 g bw/day.Results: There was a significant difference of SOD levels in each group (p=0.00) except between group K- and P1. Giving purple fleshed sweet potatoes increased SOD levels as much as 85.81±4.59 (P1). The K+ group had the lowest SOD level 22.34±3.98. The SOD level for K- group and P2 group was 82.27±4.59 and 67.73±6.68 respectively.Conclusion: The highest SOD level is on the treatment 1 group which is administered with purple fleshed sweet potato.

EFFECT OF HEAT TREATMENT ON THE ANTIOXIDANT ACTIVITIES OF TWO CULTIVARS OF SWEET POTATOES

2016 ◽  
Vol 78 (6-12) ◽  
Author(s):  
Amir H. M. S. ◽  
Nurun N. ◽  
Nida Iqbal ◽  
Nur F. R. ◽  
Lee L. H. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Antioxidant Activities ◽  
Sweet Potatoes ◽  
Moist Heat ◽  
Dry Heat ◽  
Significant Difference ◽  
Ic50 Values ◽  
Purple Sweet Potato

Natural sources of antioxidants are derived from fruits, vegetables and wine, whilst artificial supplements are from teas and spices. Sweet potato (Ipomoea batatas) is an excellent natural source of vitamins and minerals, and likely a great source of antioxidant. The objective of this study  is to analyze the antioxidant activity of orange sweet potato (Vitato) and  purple sweet potato (All purple), prepared as heat dry and  moist heat for 30 minutes at 100oC. All the samples were obtained from Pasir Puteh and MARDI Telong, Bachok, Kelantan, respectively. Both samples were soaked into methanol to obtain the crude extract prior to analyzing for antioxidant activity by using 2, 2-diphenyl-1-picryl hydrazyl (DPPH). IC50 values of dry heat and moist heat Vitato were 0.40mg/L and 0.20mg/L while dry heat and moist heat, All purple were 0.32mg/L and 0.19mg/L, respectively. Both moist heat samples enjoyed higher scavenging activities compared to dry heat samples. However, the All purple sample of moist heat is the most superior one. Significant difference of IC50values between dry heat and moist heat sample differ significantly. Thus, this study clearly demonstrated that moist heat sweet potato exhibited  excellent increase in antioxidant activity.

scholarly journals Effect of single clove garlic on clinical symptoms of photoaging in wistar rats exposed with UV-B

2019 ◽  
Vol 2 (2) ◽  
pp. 40-44
Author(s):  
Nyoman Pratiwi Hapsari Dewi ◽  
I Gusti Ayu Dewi Ratnayanti ◽  
I Gusti Kamasan Nyoman Arijana ◽  
Ni Made Linawati
Keyword(s):  
Clinical Symptom ◽  
Wistar Rats ◽  
Clinical Symptoms ◽  
Light Exposure ◽  
Premature Aging ◽  
The Other ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Photoaging is a premature aging that occurs on skin due to the ultraviolet light exposure that causes the emergence of clinical symptoms, one of which is wrinkle. One kind of material that can prevent photoaging is antioxidant. A single clove garlic has the highest antioxidant activity compared to the other materials. Aim: This research aimed at investigating the effectivity of single clove garlic in inhibiting the clinical symptom of photoaging. Method: This research was an experimental research, utilizing pre-post-test control group design. The used sample were 30 male wistar rats which were divided into 6 experimental groups. All groups were exposed to UV-B light with the amount of 840 mJ/cm2. Control group (P1) was only exposed to UV-B while the other groups were treated with placebo cream (P2), sunblock (P3), 5% garlic cream (P4), 10% garlic cream (P5) and 20% garlic cream (P6) respectively. The clinical symptom in the form of wrinkle was observed by using dermascope and the observations were categorized based on Glogau Scale. The statistical analysis utilized Wilcoxon and Kruskal Wallis test which was followed by Mann Whitney test. Result: The result of the research showed that there was significant difference on the apparent wrinkle on P1 group and P3, P5 and P6. Meanwhile the comparison between P1, P2 and P4 was not significant. Conclusion: The single clove garlic extract can prevent photoaging and has the similar protective effect for the skin as of sunblock.

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