scholarly journals Radiographic and histological evaluation of bisphosphonate alendronate and metotrexate effects on rat mandibles inoculated with Walker 256 carcinosarcoma

2007 ◽  
Vol 22 (6) ◽  
pp. 457-464 ◽  
Author(s):  
Ana Paula Negreiros Nunes Alves ◽  
Cláudia do Ó Pessoa ◽  
Letícia Costa-Lotufo ◽  
Manoel Odorico Moraes Filho
Keyword(s):  
Histological Analysis ◽  
Histological Evaluation ◽  
Control Group ◽  
Radiographic Images ◽  
Walker 256 Carcinosarcoma ◽  
Central Regions ◽  
Malignant Lesions ◽  
Walker 256 ◽  
Bone Trabeculae ◽  
Osteoid Formation

PURPOSE: To investigate the effects of bisphosphosnate alendronate (ALD) and metotrexate (MTX) on an experimental model of Walker 256 carcinosarcoma developed in the oral cavity of rats. METHODS Walker 256 carcinosarcoma cell suspension (0,1 mL) containing 10(6) cell/mL was implanted in the alveoli of the first and second molars. The animals were divided and treated with saline, MTX, ALD, and MTX plus ALD. Later, the animals were sacrificed, the tumors were measured and the mandibles were removed for radiographic and histological analysis. RESULTS: In the control group, the radiographic images demonstrated radioluscency with poorly defined borders, and the microscopic examination revealed tumor infiltration into the peripheral and central regions of the bone. Areas of necrosis were commonly seen. In the treated groups with ALD, associated or not with MTX, the radiographic analysis revealed circumscribed tumor-induced osteolysis and various degrees of radiotransparence; while, histologically, preserved bone trabeculae with osteoid formation was observed among malignant cells. CONCLUSION: The bisphosphonate alendronate exherted an osteoprotective effect and induced bone neoformation on the Walker 256 carcinosarcoma inoculated in rat mandibles. The combination of metotrexate with bisphosphonate alendronate is more successful than treatment with the agents alone in controlling the growth of neoplastic cells and in stimulating reactive new bone. Therefore, this may be an alternative treatment to malignant lesions of maxillaries with osteolysis.

scholarly journals Action of tacrolimus on Wistar rat kidneys implanted with Walker 256 carcinosarcoma

2010 ◽  
Vol 25 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Cristiano Machado Inácio ◽  
Ulrich Andréas Dietz ◽  
Osvaldo Malafaia ◽  
Jurandir Marcondes Ribas Filho ◽  
Paulo Afonso Nunes Nassif ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Statistical Significance ◽  
Control Group ◽  
Walker 256 Carcinosarcoma ◽  
Tumor Group ◽  
Male Wistar Rats ◽  
Walker 256 ◽  
The Right

PURPOSE: To evaluate the development of Walker 256 tumor in male Wistar rats treated with tacrolimus using an experimental kidney tumor model. METHODS: 40 male Wistar rats were divided into four groups: Tumor group (TU) (n=10), Tacrolimus-Tumor group (TT) (n=10), Tacrolimus group (TC) (n=10) and Control group (C) (n=10). Treatment with tacrolimus was performed in groups TT and TC. Under anesthesia, the right kidney of each animal of TU and TT was accessed through a supraumbilical incision and inoculated with a 0.1mL solution containing 2x10(6) tumor cells (Walker 256 carcinosarcoma tumor cells). Group TC was treated with a saline solution. All the animals of groups TC and TT were treated with tacrolimus (5mg/kg/day) by gavage for 15 days. TU group animals received saline by gavage for 15 days. On the 15th postoperative day, all animals were submitted to euthanasia and blood sampling for analysis of serum creatinine (Cr) and blood urea nitrogen (BUN). Abdominal gross examination was performed, the right kidney removed and prepared for histological analysis by hematoxylin-eosin staining. The resulting data were submitted to statistical analysis by ANOVA. RESULTS: Statistical significance was found when comparing creatinine level between groups TU, TT and TC -TT group culminated with a marked increased in creatinine levels (Cr=1.013 ± 0.3028 mg/mL), TU group (Cr=0.5670 ± 0.03536 mg/dL) P=0.00256, TC group (Cr =0.711 ± 0.1653 mg/mL) P= 0.02832. Statistical significance was found when comparing BUN levels in TT group (71.32 ± 17.14 mg/mL), compared with TU group (45.83 ± 5.046 mg/dL), P=0.000318. There were no statistically significant differences between groups TT and TC (61.23 ± 9.503 mg/mL) P=0.7242. Histological analysis showed a poor evolution in TT group with multiple foci of hemorrhage and cortical invasion by the Walker tumor. CONCLUSION: The Tacrolimus-treated group developed a more aggressive tumor and a drug-related nephrotoxic effect.

scholarly journals Histological evaluation of the effects of corticotomy on induced orthodontic movement in rats

2017 ◽  
Vol 46 (6) ◽  
pp. 368-373 ◽  
Author(s):  
Patrícia Pigato SCHNEIDER ◽  
Lais Pavan SILVA ◽  
Fernando José Lopes de Campos CARVALHO ◽  
Luís Filipe Siu LON ◽  
Luiz Gonzaga GANDINI JÚNIOR
Keyword(s):  
Histological Analysis ◽  
Tooth Movement ◽  
Initial Period ◽  
Invasive Procedure ◽  
Histological Evaluation ◽  
Control Group ◽  
Coil Spring ◽  
Orthodontic Movement ◽  
Spring Force ◽  
Movement Group

Abstract: Introduction: Alveolar corticotomy is a surgical procedure used to increase the velocity of tooth movement. Objective Identify histological evidence of the effect of corticotomy on orthodontic movement in rats. Material and method Forty-five Wistar rats (Rattusnorvegicus Albinus) were equally divided into three groups: Control Group (CG) - no tooth movement or corticotomy; Movement Group (MG) - tooth orthodontic movement only; and Corticotomy and Movement Group (CMG) - tooth orthodontic movement surgically assisted by corticotomy. In the CMG, surgical procedures consisted in an incision in the palatal, reaching from the mesial to the distal regions of the maxillary right first molar. Tooth movement in the MG and CMG was applied with coil spring force of 40 gF from the maxillary right first molar to the maxillary right incisor. The rats were sacrificed at days 1, 3, and 7, and histological sections were performed to evaluate the counting of osteoblasts and osteoclasts throughout the areas of tension and pressure. Result Histological analysis showed that the CMG presented better cell response to bone neoformation compared with that of the other groups. Greater proliferation of osteoclasts was observed in areas of pressure on day 3, resulting in increased reabsorption, whereas greater proliferation of osteoblasts was observed in areas of tension on day 1, indicating increased bone formation. Conclusion Differences between the treated groups occurred only in the initial period of tooth movement. Therefore, the changes caused by corticotomy are not significant in orthodontic movement to justify this invasive procedure.

scholarly journals Quercetin reduces tendon adhesion in rat through suppression of oxidative stress

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Yuan Liang ◽  
Keteng Xu ◽  
Pei Zhang ◽  
Jiale Zhang ◽  
Pengtao Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Histological Analysis ◽  
Histological Evaluation ◽  
Control Group ◽  
Biological Functions ◽  
Tendon Tissue ◽  
Safe Strategy ◽  
Prevention And Treatment ◽  
And Control ◽  
Clinical Problems

Abstract Background Tendon adhesion is one of the most common clinical problems, which poses a considerable challenge to orthopedics doctors. Quercetin (QUE) as a popular drug at present, it has various biological functions, including anti-inflammatory, anti-ischemic, anti-peroxidation, and antioxidant. The purpose of this study was to investigate the effect of quercetin on tendon adhesion and whether quercetin can inhibit oxidative stress. Method Thirty-six rats were randomly divided into three groups, including control group, low QUE (50 mg/kg/day) group, and high QUE (100 mg/kg/day) group. After 1 week, the levels of SOD, MDA and GPx were measured. The degree of tendon adhesion was assessed by macroscopic evaluation and histological evaluation. After 4 weeks. Besides, the pharmacological toxicity of quercetin to main organs were evaluated by histological analysis. Results The extent of superoxide dismutase (SOD) and glutathione peroxidase (GPx) of tendon tissue in high QUE group was significantly higher than those of low QUE group and control group. And the extent of malondialdehyde (MDA) of tendon tissue in high QUE group was significantly lower than that of low QUE group and control group. By macroscopic evaluation and histological analysis, the extent of tendon adhesion in high QUE group was lower than low QUE group and control group. However, there were no significant changes of the major organs through histological analysis. Conclusions Quercetin may be a good and safe strategy in preventing tendon adhesion. But further clinical research is needed before its recommendation in the prevention and treatment of tendon adhesion.

scholarly journals Histological analysis of the repair of dural lesions with silicone mesh in rats subjected to experimental lesions

2015 ◽  
Vol 13 (4) ◽  
pp. 567-573
Author(s):  
Fernando William Figueiredo da Rosa ◽  
Pedro Henrique Isoldi Pohl ◽  
Ana Maria Amaral Antônio Mader ◽  
Carla Peluso de Paiva ◽  
Aline Amaro dos Santos ◽  
...  
Keyword(s):  
Foreign Body ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Histological Evaluation ◽  
Control Group ◽  
Collagen Membrane ◽  
Dural Tear ◽  
Dural Repair ◽  
Score System ◽  
Bovine Collagen

ABSTRACT Objective To evaluate inflammatory reaction, fibrosis and neovascularization in dural repairs in Wistar rats using four techniques: simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture. Methods Thirty Wistar rats were randomized in five groups: the first was the control group, submitted to dural tear only. The others underwent durotomy and simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture. Animals were euthanized and the spine was submitted to histological evaluation with a score system (ranging from zero to 3) for inflammation, neovascularization and fibrosis. Results Fibrosis was significantly different between simple suture and silicon mesh (p=0.005) and between simple suture and mesh with suture (p=0.015), showing that fibrosis is more intense when a foreign body is used in the repair. Bovine membrane was significantly different from mesh plus suture (p=0.011) regarding vascularization. Inflammation was significantly different between simple suture and bovine collagen membrane. Conclusion Silicon mesh, compared to other commercial products available, is a possible alternative for dural repair. More studies are necessary to confirm these findings.

A Histological Evaluation of Novel Cyanoacrylate-Based β-TCP Composite in Rat Calvarial Defects

2006 ◽  
Vol 309-311 ◽  
pp. 1133-1136 ◽  
Author(s):  
Sang Bae Lee ◽  
Kyeong Jun Park ◽  
Doug Youn Lee ◽  
Jeong Jong Park ◽  
Jae Sun Hwang ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Weight Ratio ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Control Group ◽  
Filling Material ◽  
Fibrous Connective Tissue ◽  
Implantation Time ◽  
Filling Materials ◽  
Calvarial Defects

In this study, the osteoconductive properties of novel cyanoacrylate-based filling materials for bone defect were evaluated. A new filling material was prepared by mixing Histoacryl® and acid-treated -tricalcium phosphate ( -TCP). Mixing weight ratio of acid-treated -TCP to Histoacryl® was 5:1. 12 male Spraque-Dawley rats were used in this study. The animals were divided into 4 groups. Critical-sized calvarial defects (8 mm) were created in 9 animals, and then the defects were treated with dense pellet specimen, porous cement-like specimen, and untreated defect for surgical control group. Augmentation treatments were carried out in 3 animals. Histological analysis revealed excellent ostgeoconductive properties of new filling materials. But, some of -TCP particle in the cement-like group were encapsulated by fibrous connective tissue. For the dense pellet group and augmentation treatment group, shape and stability were better maintained during the implantation time than cement like group. These results indicate that our novel -TCP/Histoacryl® composite have the potential to serve as filling materials for bone defects in the dental and plastic surgery.

Motor Recovery after Chronic Spinal Cord Transection in Rats: A Proof-of-Concept Study Evaluating a Combined Strategy

2019 ◽  
Vol 18 (1) ◽  
pp. 52-62 ◽  
Author(s):  
Antonio Ibarra ◽  
Erika Mendieta-Arbesú ◽  
Paola Suarez-Meade ◽  
Elisa García-Vences ◽  
Susana Martiñón ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Treatment Strategies ◽  
Chronic Phase ◽  
Motor Recovery ◽  
Neural Regeneration ◽  
Histological Evaluation ◽  
Control Group ◽  
Proof Of Concept ◽  
Sprague Dawley ◽  
Concept Study

Background: The chronic phase of Spinal Cord (SC) injury is characterized by the presence of a hostile microenvironment that causes low activity and a progressive decline in neurological function; this phase is non-compatible with regeneration. Several treatment strategies have been investigated in chronic SC injury with no satisfactory results. OBJECTIVE- In this proof-of-concept study, we designed a combination therapy (Comb Tx) consisting of surgical glial scar removal plus scar inhibition, accompanied with implantation of mesenchymal stem cells (MSC), and immunization with neural-derived peptides (INDP). Methods: This study was divided into three subsets, all in which Sprague Dawley rats were subjected to a complete SC transection. Sixty days after injury, animals were randomly allocated into two groups for therapeutic intervention: control group and animals receiving the Comb-Tx. Sixty-three days after treatment we carried out experiments analyzing motor recovery, presence of somatosensory evoked potentials, neural regeneration-related genes, and histological evaluation of serotoninergic fibers. Results: Comb-Tx induced a significant locomotor and electrophysiological recovery. An increase in the expression of regeneration-associated genes and the percentage of 5-HT+ fibers was noted at the caudal stump of the SC of animals receiving the Comb-Tx. There was a significant correlation of locomotor recovery with positive electrophysiological activity, expression of GAP43, and percentage of 5-HT+ fibers. Conclusion: Comb-Tx promotes motor and electrophysiological recovery in the chronic phase of SC injury subsequent to a complete transection. Likewise, it is capable of inducing the permissive microenvironment to promote axonal regeneration.

scholarly journals Local and systemic inflammation after implantation of a novel iron based porous degradable bone replacement material in sheep model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bernd Wegener ◽  
Maik Behnke ◽  
Stefan Milz ◽  
Volkmar Jansson ◽  
Christian Redlich ◽  
...  
Keyword(s):  
Mechanical Stability ◽  
Bone Substitutes ◽  
Histological Evaluation ◽  
Control Group ◽  
Blood Levels ◽  
Porous Iron ◽  
Sheep Model ◽  
Bone Replacement ◽  
Alloy Particles ◽  
Iron Based

AbstractDespite the high potential of healthy bone to regenerate, the reconstruction of large bone defects remains a challenge. Due to the lack of mechanical stability of existing bone substitutes, recently developed degradable metallic alloys are an interesting alternative providing higher load-bearing capabilities. Degradable iron-based alloys therefore might be an attractive innovation. To test the suitability of a newly-designed iron-based alloy for such applications, an animal experiment was performed. Porous iron-based degradable implants with two different densities and a control group were tested. The implants were positioned in the proximal tibia of Merino sheep. Over a period of 6 and 12 months, blood and histological parameters were monitored for signs of inflammation and degradation. In the histological evaluation of the implants` environment we found degraded alloy particles, but no inflammatory reaction. Iron particles were also found within the popliteal lymph nodes on both sides. The serum blood levels of phosphorus, iron and ferritin in the long term groups were elevated. Other parameters did not show any changes. Iron-based degradable porous bone replacement implants showed a good biocompatibility in this experiment. For a clinical application, however, the rate of degradation would have to be significantly increased. Biocompatibility would then have to be re-evaluated.

scholarly journals Effects of Dietary Inclusion of Bilberry and Walnut Leaves Powder on the Digestive Performances and Health of Tetra SL Laying Hens

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 823 ◽  
Author(s):  
Roua Gabriela Popescu ◽  
Sorina Nicoleta Voicu ◽  
Gratiela Gradisteanu Pircalabioru ◽  
Alina Ciceu ◽  
Sami Gharbia ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Specific Activity ◽  
Basal Diet ◽  
Intestinal Content ◽  
Final Concentration ◽  
Control Group ◽  
Villus Height ◽  
Tissue Samples ◽  
Walnut Leaves ◽  
Digestive Health

The purpose of this study was to examine the effects of dietary inclusion of two additives at the final concentration of 0.5% bilberry (E1) and 1% walnut (E2) leaves powder in the basal diet on digestive health of hens. A total number of 90 Tetra SL hens were divided into two experimental groups (E1 and E2) and one control group (C) consisting of 30 hens each. After four weeks, 10 hens of each group were sacrificed and tissue samples and intestinal content were taken from the duodenum, jejunum, and cecum in order to perform histological, enzymatic, and microbiota analyses. In groups E1 and E2, the histological analysis showed a significant increase of villus height, resulting probably in increased absorption of nutrients in duodenum and jejunum. A decrease in the specific activity of alpha-amylase and trypsin in E1 and E2 for both duodenum and jejunum compared to the control one was also recorded. In addition, the maltase and invertase specific activity in duodenum increased, a tendency that was kept for maltase but not for invertase in jejunum. The cecal microbiota of E1 and E2 individuals was characterized by an increase of Firmicutes and Lactobacilli and a decrease of Enterobacteriaceae. In conclusion, our results indicate that bilberry and walnut leaves additives in feed may improve the health status of the poultry gastrointestinal tract.

scholarly journals Flaxseed, olive and fish oil influence plasmatic lipids, lymphocyte migration and morphometry of the intestinal of Wistar rats

2010 ◽  
Vol 25 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Damiana Diniz Rosa ◽  
Regiane Lopes de Sales ◽  
Luis Fernando de Sousa Moraes ◽  
Fabíola Cesário Lourenço ◽  
Clóvis Andrade Neves ◽  
...  
Keyword(s):  
Fish Oil ◽  
Intestinal Mucosa ◽  
Wistar Rats ◽  
Density Lipoprotein ◽  
Parameter Analysis ◽  
Male Rats ◽  
Histological Evaluation ◽  
Control Group ◽  
Soy Oil ◽  
Lymphocyte Migration

PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.

Osteoarthritis of the Ankle and Foot Complex in Former Greek Soccer Players

2011 ◽  
Vol 4 (6) ◽  
pp. 338-343 ◽  
Author(s):  
Elias Armenis ◽  
Nikolaos Pefanis ◽  
Georgios Tsiganos ◽  
Panagiotis Karagounis ◽  
Panagiotis Baltopoulos
Keyword(s):  
Clinical Manifestations ◽  
Cartilage Degeneration ◽  
Joint Disease ◽  
Soccer Players ◽  
Control Group ◽  
Radiographic Images ◽  
Former Athletes ◽  
Level Ii ◽  
Analysis Of The Images ◽  
Ankle And Foot

Sports activities cause increased loads in elite athletes’ joints. Current scientific knowledge highlights the importance of applied mechanical loads on the physiology and pathophysiology of the articular cartilage. Thus, it is possible that sporting activity has a role in the development of osteoarthritis (OA), a painful and damaging joint disease. The aim of the present study was to investigate and record osteoarthritic alterations in the ankle and foot complex in former Greek soccer players and also compare them with those in the general population. The study sample consisted of 170 male, former elite soccer players, aged between 42 and 55 years (mean = 49.8 years, standard deviation [SD] = 7.4). A control group of 132 men, aged between 42 and 55 years (mean, 50.7 years, SD = 9.9), with no regular athletic activity were examined. The development of osteoarthritic alterations was recorded through a questionnaire and clinical and radiological examination. Radiographic analysis of the images in former athletes group showed not only more signs of cartilage degeneration in comparison with the control group (P < .05) but also similar clinical manifestations (pain and impaired mobility; P > .05). Osteophyte formation is a frequent disease among former soccer players—with variations on radiographic images—but it does not appear in their clinical picture. However, it is likely that both spurs and subchondral sclerosis (main findings) are preclinical manifestations of OA. Levels of Evidence: Prognostic, Level II

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