scholarly journals Electro-acupuncture reduces the need for additional anesthetics in experimental studies

2010 ◽  
Vol 25 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Agamenon Honório Silva ◽  
Alberico Ximenes do Prado Neto ◽  
Sérgio Botelho Guimarães
Keyword(s):  
Stainless Steel ◽  
Electrical Stimulation ◽  
Wistar Rats ◽  
Experimental Studies ◽  
Needle Insertion ◽  
Additional Treatment ◽  
Human Right ◽  
Beneficial Effects ◽  
Group Control ◽  
Male Wistar Rats

PURPOSE: To evaluate the possible beneficial effects of electro-acupuncture in rats subjected to ketamine/xylazine (KX) intra-peritoneal (i.p.) anesthesia. METHODS: Forty-eight male Wistar rats were distributed in four equal groups. All rats received i.p. injections of ketamine (90 mg/kg) +xylazine (10 mg/kg) anesthesia. Basal values group (control) rats (BV) received no additional treatment. The equivalent of the human right ST36 (Zusanli) and CV-12(Zhongwan) acupoints were chosen for needling and electrical stimulation. AC rats were needled with sterilized disposable stainless steel needles at right ST36 and CV12 acupoints; needles were retained for 30 minutes. EAC10 rats, after needle insertion as described, had electrodes connected to both needles and to an electro stimulator model NKL EL-608; pulsed square waves, 10 Hz, 10 mA, was applied for 30 minutes. EAC100 rats were submitted to EA as described. However, a greater frequency (100 Hz) was used. RESULTS: Thirty-seven rats remained under adequate anesthetic level during the experiment. However, maintenance anesthesia was required by 11 rats. Need for additional anesthesia decreased to 9.1% in EAC100 rats compared to BV (36.3%). CONCLUSION: Both the AC and the EAC10/100 prolong the anesthetic effect of the combination Ketamine-xylazine in rats, allowing longer duration of anesthesia with a lower dose of anesthetic, thereby reducing the occurrence of complications.

Biochemical and histopathological effects of low dose vanadium in the healing of acetic acid-induced colitis in male wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Tosan Peter Omayone ◽  
Samuel Babafemi Olaleye
Keyword(s):  
Acetic Acid ◽  
Untreated Control ◽  
Wistar Rats ◽  
Low Dose ◽  
Healing Process ◽  
Beneficial Effects ◽  
Male Wistar Rats ◽  
Haematological Changes ◽  
Therapeutic Properties ◽  
Histopathological Effects

Abstract Objectives Vanadium has been reported to possess relevant therapeutic properties such as anti-diabetic and anti-tumoral. This study aimed at determining the effects of vanadium on experimentally induced colitis in rats. Methods Forty-five male Wistar rats (103 ± 3.90 g, n=15) were used for this study and were divided into three groups. Group 1 (Untreated control) had nothing added to their drinking, while groups 2 and 3 received sodium metavanadate at a dose of 50 and 200 mg/L respectively in their drinking water for 10 weeks. Colitis was thereafter induced by intra colonic administration of 1.50 mL of 6% acetic acid. Animals were sacrificed on day 0 (pre-induction), three- and seven-days post induction. Blood samples were collected for haematological variables and the distal 8 cm of the colon was collected for macroscopic, histological and biochemical (malondialdehyde-MDA, superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase- GPx and nitrite concentration- NO) assessment. Results Low dose vanadium proved beneficial in ameliorating acetic acid-induced colitis by improving both histopathological and haematological changes. Gross observation showed a faster healing rate in vanadium treated groups (50 and 200 mg/L) compared with untreated control at day 3 (40 and 26.20 vs. 2.50%) and day 7 (80 and 66.70 vs. 42%) respectively. Vanadium also appears to exert its beneficial effects on acetic acid-induced colitis via up regulation of antioxidant enzymes (SOD, CAT, GPx) and NO while decreasing the over production of MDA. Conclusions Vanadium at small concentration functions as an essential trace element and may be able to promote healing process during ulcerative colitis.

Cyclophosphamide-induced reproductive toxicity: Beneficial effects of Helichrysum odoratissimum (Asteraceae) in male Wistar rats

2019 ◽  
Vol 17 (5) ◽  
pp. 366-373 ◽  
Author(s):  
Pierre Watcho ◽  
Ismaelle Rosine Mpeck ◽  
Patrick Brice Deeh Defo ◽  
Modeste Wankeu-Nya ◽  
Esther Ngadjui ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Reproductive Toxicity ◽  
Beneficial Effects ◽  
Male Wistar Rats

scholarly journals The use of electrical stimulation to enhance spinal fusion

2002 ◽  
Vol 13 (6) ◽  
pp. 1-7 ◽  
Author(s):  
Michael A. Morone ◽  
Henry Feuer
Keyword(s):  
Electrical Stimulation ◽  
Spinal Fusion ◽  
Healing Process ◽  
Experimental Studies ◽  
Spinal Instrumentation ◽  
Fusion Rate ◽  
Beneficial Effects ◽  
Osseous Union ◽  
Spinal Segments ◽  
Spinal Arthrodesis

The goal of spinal arthrodesis is a solid osseous union across one or more spinal segments. A solid bone union after arthrodesis is commonly known as a spinal fusion. Surgeons have begun to understand the biomechanical and biological factors that influence the bone-healing process. One of the most commonly used adjuncts is spinal instrumentation. Instrumentation has increased the spinal fusion rate; however, fusion failure (that is, nonunion or pseudarthrosis) remains significant. A less commonly used adjunct is electrical stimulation (ES). Investigators in experimental studies have demonstrated the beneficial effects of ES on increasing the fusion rate. In this review the authors discuss the evidence concerning the benefits of ES as an adjunct to spinal arthrodesis. In addition, the different types of ES devices are described along with the current experimental and clinical evidence for each type of device.

scholarly journals Technical report: Simple method of animal stroke model of luminal occlusion of middle cerebral artery in Indonesia

2019 ◽  
Vol 10 ◽  
pp. 143
Author(s):  
Feda Anisah Makkiyah ◽  
Wismaji Sadewo
Keyword(s):  
Middle Cerebral Artery ◽  
Cerebral Artery ◽  
Wistar Rats ◽  
Experimental Studies ◽  
Artery Occlusion ◽  
Stroke Model ◽  
Simple Method ◽  
Technical Report ◽  
Male Wistar Rats ◽  
Cerebral Artery Occlusion

Background: Although there are many experimental studies describing the suture method of middle cerebral artery occlusion (MCAO) in rats, this is still a new procedure in Indonesia and the techniques for applying this stroke model in animal research are not well known. There has been a perception in Indonesian scientific community that the technique would be difficult and require advanced equipment. The aim of this study is to demonstrate that it is possible to perform the technique with minimal resources using simple method and basic surgical loupe equipment. Methods: A total of 30 male Wistar rats, aged 6 months, weighing 250 g–400 g Wistar rats, were obtained from the Bandung Biofarma Pasteur. Preliminary trials were performed to gain an understanding of the detailed anatomy of the animals and to master the techniques. An ×8 loupe magnification was used for all surgical steps in this study except taking of surgical operation photos. The procedures applied simple methods, using two loop temporary knots instead of any animal vascular clips. Results: After an extensive training period, two of the 30 rats died within 4 weeks after the procedure. The effects of MCAO were confirmed clinically and by hematoxylin-eosin staining pathology slides. Conclusion: With simple methods, this MCAO procedure could be implemented in developing countries such as Indonesia without the requirement for advanced equipment.

scholarly journals Analysing the Hypolipidemic Activities of the Tea Extracts of Moringa oleifera, Glycyrrhiza glabra and their Blend at Different Concentrations, Orally Induced on Adult Male Wistar Rats.

2016 ◽  
Vol 3 ◽  
pp. 76-83
Author(s):  
Olanrewaju Roland Akinseye
Keyword(s):  
Wistar Rats ◽  
Moringa Oleifera ◽  
Glycyrrhiza Glabra ◽  
Atherogenic Index ◽  
Simultaneous Increase ◽  
Hypolipidemic Effect ◽  
Aqueous Extracts ◽  
Water Control ◽  
Beneficial Effects ◽  
Male Wistar Rats

Herbal teas ofMoringa oleifera(Moringa) andGlycyrrhiza glabra(Licorice)were known to have many beneficial effects, their possible hypolipidemic effects were investigated in this paper. Albino wistar rats were fed with aqueous extracts ofM. oleifera,G. glabraand their blend teas(10, 30 and 50 mg/kg.BW/ml). The teas were found to lower the serum cholesterol, triglycerides, VLDL, LDL, and atherogenic index at their higher concentration, but were found to slightly increase the HDL as compared to the corresponding normal healthy rats fed with clean water (control). The phytochemicals screening of the teas aqueous extract were also investigated, the chemicals (tannins, saponins, flavonoids, terpenoids and glycosides) which were known to exhibit cholesterol lowering effect by inhibiting its absorption and simultaneous increase its excretion were found to be present in the teas extract. Thus, the study demonstrates and validates thatM.oleifera,G. glabraand their blend possess hypolipidemic effect at higher concentration.

Beneficial effects of lipidic extracts of saladette tomato pomace andSerenoa repenson prostate and bladder health in obese male Wistar rats

2017 ◽  
Vol 97 (13) ◽  
pp. 4451-4458 ◽  
Author(s):  
Josué V Espinosa-Juárez ◽  
Juventino III Colado-Velázquez ◽  
Patrick Mailloux-Salinas ◽  
JML Medina-Contreras ◽  
P Valentín Correa-López ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Tomato Pomace ◽  
Beneficial Effects ◽  
Male Wistar Rats ◽  
Obese Male

scholarly journals Beneficial effects of low dose Musa paradisiaca on the semen quality of male Wistar rats

2013 ◽  
Vol 54 (2) ◽  
pp. 92 ◽  
Author(s):  
GabrielO Omotoso ◽  
C. N. B. Tagoe ◽  
OB Akinola ◽  
AS Alabi ◽  
BU Enaibe
Keyword(s):  
Wistar Rats ◽  
Low Dose ◽  
Semen Quality ◽  
Beneficial Effects ◽  
Musa Paradisiaca ◽  
Male Wistar Rats

Beneficial Effects of Melatonin and Atorvastatin on Retinopathy in Streptozocin-induced Diabetic Rats

2020 ◽  
Vol 15 (4) ◽  
pp. 396-403
Author(s):  
Mansoor Shahriari ◽  
Saeed Mehrzadi ◽  
Masood Naseripour ◽  
Iman Fatemi ◽  
Azam Hosseinzadeh ◽  
...  
Keyword(s):  
Group Treatment ◽  
Wistar Rats ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Beneficial Effects ◽  
Vascular Congestion ◽  
Therapeutic Value ◽  
Group Control ◽  
Group Control Group

Objective: The present study was designed to evaluate the effects of Atorvastatin (ATO) plus Melatonin (MEL) on streptozocin-induced Diabetic Retinopathy (DR) in rats. Methods: Diabetes was induced in Wistar rats with an intraperitoneal injection of streptozocin (50 mg/kg). Animals were randomly assigned to one of the following groups (8 rats/group): Control group, Diabetic group, Diabetic + MEL group (20 mg/kg/day), Diabetic + ATO group (10 mg/kg/day), Diabetic + MEL + ATO group (as above). Treatments were started one week after induction of diabetes and continued for 7 weeks. At the end of the experiment, angiography was performed and the rats were killed and retinas were harvested for pathological and molecular examinations. Results: Administration of MEL reduced the fluorescein leakage, MDA and ROS levels compared to diabetic group. Treatment with ATO only reduced ROS levels compared to diabetic group. In addition, administration of ATO plus MEL decreased these indices compared to the diabetic and ATO groups. Histologically, retinal vascular congestion was not observed in the combined ATO and MEL group as compared to the diabetic, ATO, and MEL groups. Conclusion: These data provide evidence for the therapeutic value of MEL in combination with ATO in clinical practice for prevention of DR.

scholarly journals Power Spectrum Features of Acupoint Bioelectricity Signal

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Jingjing Zhang ◽  
Renhuan Yu ◽  
Enlu Zhao ◽  
Quan Zhou ◽  
Shuping Gai
Keyword(s):  
Stainless Steel ◽  
Electrical Stimulation ◽  
Electrical Properties ◽  
Power Spectrum ◽  
Needle Insertion ◽  
Acupuncture Points ◽  
Electrical Signals ◽  
Signed Rank ◽  
Significant Difference ◽  
And Control

Background. Since the 1950s, many studies have been conducted on the electrical properties of acupuncture points (acupoints), especially their bio-resistance characteristics. Results of such studies have been inconclusive due to factors such as sweat gland density and compounding factors of applying electrical stimulation. In this study, a power spectrum instrument was used to assess the power spectrum and power of acupoints and nonacupoints without electrical stimulation. Using such instrumentation, specificity of electrical signals of acupoints was also explored. Methods. Thirty-six subjects (29 females, 7 males) participated in the study. Stainless steel acupuncture needles (diameter 0.35 mm; length 50 mm) were used. Five acupoints were tested: ST 36, SP 6, GB 39, GB 37, and K I9. Four control sites 0.5–1.0 cm adjacent to each acupoint were chosen. After needle insertion into the acupoint and control sites, the needles were attached to the power spectrum instrument to acquire any electrical signals. Acquire signals were analyzed using self-written software. Results. Power spectrum difference between acupoint and nonacupoint signals was 0–2 Hz. Results of t-test or signed rank sum test (α = 0.05) found that electrical signals between acupoints and nonacupoints were markedly different ( P  < 0.05). Conclusion. Acupoint bioelectricity signals are higher than adjacent nonacupoints. The most significant difference is distributed between 0 Hz and 2 Hz.

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