scholarly journals Alveolar wound healing after implantation with a pool of commercially available bovine bone morphogenetic proteins (BMPs): a histometric study in rats

2007 ◽  
Vol 18 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Romeu Felipe Elias Calixto ◽  
Juliana Mazzonetto Teófilo ◽  
Luiz Guilherme Brentegani ◽  
Teresa Lúcia Lamano-Carvalho
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Proteins ◽  
Volume Fraction ◽  
Male Rats ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Bovine Collagen ◽  
Histologic Pattern ◽  
Hematoxylin And Eosin ◽  
And Control

The capacity of a commercially available pool of bovine bone morphogenetic proteins (BMPs) to stimulate osteogenesis in the rat alveolar healing was investigated by histometric analysis. Male rats were anesthetized and had their upper incisor extracted. A pool of purified bovine BMPs adsorbed to microgranular resorbable hydroxyapatite was agglutinated with bovine collagen and saline before implantation into the alveolar socket. The implanted and control rats (n=30 per group) were sacrificed 1 to 9 weeks postoperatively, the hemi-maxillae were decalcified, processed for paraffin embedding and semi-serial longitudinal sections were obtained and stained with hematoxylin and eosin. The volume fraction of alveolar healing components was estimated by a differential point-counting method in histologic images. The results showed that in both, control and implanted rats, the alveolar healing followed the histologic pattern usually described in the literature. Quantitative data confirmed that the BMPs mixture did not stimulate new bone formation in the alveolar socket of implanted rats. These results suggest that the pool of BMPs adsorbed to hydroxyapatite and agglutinated with bovine collagen did not warrant incorporation of the osteoinductive proteins to a slow-absorption system that would allow a BMPs release rate compatible to that of new bone formation, and thus more adequate to osteoinduction.

INFLUENCE OF ETHYLENE OXIDE STERILIZATION ON NEW BONE FORMATION INDUCED BY BOVINE BONE MORPHOGENETIC PROTEIN

2000 ◽  
Vol 04 (04) ◽  
pp. 287-295
Author(s):  
Tarmo Pekkarinen ◽  
T. Sam Lindholm ◽  
Aulis Marttinen ◽  
Oili Hietala ◽  
Pekka Jalovaara
Keyword(s):  
Bone Formation ◽  
Ethylene Oxide ◽  
Bone Morphogenetic Proteins ◽  
Exposure Time ◽  
Clinical Settings ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Oxide Concentration ◽  
Different Temperatures

Bone morphogenetic proteins (BMPs) have the capacity to induce and accelerate bone regeneration. Before experimental and clinical settings, BMP must be sterilized. Ethylene oxide (EO) gas sterilizations at different temperatures are commonly used but the effects of that on the osteoinductive capacity of BMP have been the subject of controversy. Here, we investigated the effects of three different EO sterilization methods on the osteoinductivity of partially purified native bovine BMP (bBMP). Gelatin capsules containing 3 mg of bBMP were sterilized as follows: (i) manually inside a dessicator with 12% EO spray (20°C, exposure time 2 h); (ii) with an EO gas sterilizer (Steri-Vac 4XL, temperature 29°C, exposure time 4 h 10 min, ethylene oxide concentration 860 mg/l); (iii) with an EO gas sterilizer (Steri-Vac 5XL, temperature 42°C, exposure time 3 h, ethylene oxide concentration 700 mg/l). The sterilization processes were monitored with samples of Bacillus subtilis (3M, Attest 1264). Osteoinductivity of bBMP was verified by bioassay. After 21 days of implantation of bBMP into the muscle pouches of mice, the animals were killed and new bone formation was measured radiographically and histologically. The EO sterilization techniques used did not significantly decrease the osteoinductive activity of BMP. It is concluded that commercial EO gas equipment sterilization is effective for sterilized BMP and does not decrease the osteoinductive capacity of bovine BMP.

scholarly journals Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite

2004 ◽  
Vol 15 (3) ◽  
pp. 175-180 ◽  
Author(s):  
Gabriel Ramalho Ferreira ◽  
Tania Mary Cestari ◽  
José Mauro Granjeiro ◽  
Rumio Taga
Keyword(s):  
Foreign Body ◽  
Bone Formation ◽  
Bone Morphogenetic Proteins ◽  
Bone Repair ◽  
Critical Size ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Body Type ◽  
Granulomatous Reaction ◽  
Post Surgery

The ability of a pool of bovine bone morphogenetic proteins bound to synthetic microgranular hydroxyapatite (BMPb-HA) to stimulate bone repair was determined in rat critical size defects. An 8-mm diameter defect was created in the calvaria of 25 rats. In 15 rats, the defects were filled with BMPb-HA homogenized with blood (experimental group), and in 10 rats the defects were filled only with blood clots (control). The calvariae of experimental rats were collected 1, 3 and 6 months after surgery and of the control rats at the end of surgery and 6 months thereafter. The morphometric results obtained in the radiographs showed an absence of new bone formation at 1 and 3 months post-surgery and, histologically, the defects were filled with fibrous connective tissue and numerous foci of a foreign body-type granulomatous reaction around hydroxyapatite agglomerates. At the end of 6 months, the number and size of the granulomatous foci decreased and the area of the defects was reduced by 22% compared to the 0-hour control due to the formation of new bone at their borders, although the mean area was similar to the 6-month control. We conclude that the use of BMPb-HA in the treatment of critical size bone defects of the rat skull leads to the formation of a foreign body-type granulomatous reaction that markedly inhibits new bone formation, suggesting that synthetic microgranular hydroxyapatite does not represent a good carrier for BMP-induced bone formation.

scholarly journals Bone defect regeneration with bioactive glass implantation in rats

2004 ◽  
Vol 12 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Nelson Luiz de Macedo ◽  
Fábio da Silva Matuda ◽  
Luís Guilherme Scavonne de Macedo ◽  
Michel Borges Gonzales ◽  
Suzana Martins Ouchi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bioactive Glass ◽  
Bone Defects ◽  
Male Rats ◽  
New Bone Formation ◽  
Osteoconductive Property ◽  
Histological Processing ◽  
Hematoxylin And Eosin ◽  
Bone Trabeculae ◽  
Mature Bone

The main goal of this study was to histologically evaluate the healing of surgically created defects on the tibiae of adult male rats after implantation of two types of bioactive glass. Sixteen adult Wistar rats (body weight of 300g) were divided into two groups: PerioGlas (PG) (n=8) and BioGran (BG) (n=8). Unicortical bone defects with 3-mm diameter were performed in both tibiae of the animals and filled with two types of glass particles. The rats were then sacrificed at 7, 14, 30 and 60 days, and the tissues were prepared for histological processing, sectioning, and staining with hematoxylin and eosin, as well as Mallory trichrome, and analyzed under light microscope. Within 7-14 days, both groups presented connective tissue septa with new bone formation, more intense in the PG group. In the subsequent periods (30 and 60 days), both groups presented more mature bone tissue around the glass particles. Bone trabeculae formed in all experimental periods were juxtaposed to the glass particles. It can be concluded that both materials promoted comparable bone formation over the entire extension of the defect, independently of the size of the granules, thus confirming their biological osteoconductive property.

scholarly journals Bone Morphogenetic Proteins, Carriers, and Animal Models in the Development of Novel Bone Regenerative Therapies

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3513
Author(s):  
Nikola Stokovic ◽  
Natalia Ivanjko ◽  
Drazen Maticic ◽  
Frank P. Luyten ◽  
Slobodan Vukicevic
Keyword(s):  
Animal Models ◽  
Bone Formation ◽  
Bone Morphogenetic Proteins ◽  
Animal Experiments ◽  
Inorganic Materials ◽  
New Bone Formation ◽  
Segmental Bone ◽  
Posterolateral Spinal Fusion ◽  
Regenerative Therapies ◽  
Observation Period

Bone morphogenetic proteins (BMPs) possess a unique ability to induce new bone formation. Numerous preclinical studies have been conducted to develop novel, BMP-based osteoinductive devices for the management of segmental bone defects and posterolateral spinal fusion (PLF). In these studies, BMPs were combined with a broad range of carriers (natural and synthetic polymers, inorganic materials, and their combinations) and tested in various models in mice, rats, rabbits, dogs, sheep, and non-human primates. In this review, we summarized bone regeneration strategies and animal models used for the initial, intermediate, and advanced evaluation of promising therapeutical solutions for new bone formation and repair. Moreover, in this review, we discuss basic aspects to be considered when planning animal experiments, including anatomical characteristics of the species used, appropriate BMP dosing, duration of the observation period, and sample size.

scholarly journals The use of solvent-preserved human and bovine cancellous bone blocks for lateral defect augmentation - an experimental controlled study in vivo

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Lara Schorn ◽  
Tim Fienitz ◽  
Kathrin Berndsen ◽  
Norbert R. Kübler ◽  
Henrik Holtmann ◽  
...  
Keyword(s):  
Bone Formation ◽  
Cancellous Bone ◽  
Bone Substitute ◽  
Bone Matrix ◽  
Human Bone ◽  
Controlled Study ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Bone Substitute Material ◽  
Substitute Material

Abstract Background The aim of this study was to compare new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material in laterally augmented alveolar bone defects using allogeneic, pre-treated and cleaned human bone blocks (tested in dogs, therefore considered to be xenogeneic), and pre-treated and cleaned bovine cancellous bone blocks, both with and without a collagen membrane in order to evaluate their augmentative potential. Methods Thirty-two critical size horizontal defects were prepared in the mandible of 4 adult foxhound dogs (8 per dog, 4 on each side). After 3 months of healing, the defects were laterally augmented in a split-mouth-design with either human (HXB) or bovine solvent-preserved bone blocks (BXB). Afterwards, defects were randomly covered with a bovine collagenous membrane (HXB + M, BXB + M). After a healing interval of 6 months, percentages of new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material were compared. Results Results showed little new bone formation of up to 3.7 % in human bone blocks (HXB 3.7 % ± 10.2, HXB + M 0.3 %± 0.4, BXB, 0.1 % ± 0.8, BXB + M 2.6 % ± 3.2, p = > 0.05). Percentages of fibrous encapsulation were higher in human bone blocks than in bovine bone blocks (HXB 71.2 % ± 8.6, HXB + M 73.71 % ± 10.6, BXB, 60.5 % ± 27.4, BXB + M 52.5 % ± 28.4, p = > 0.05). Resorption rates differed from 44.8 % in bovine bone blocks covered with a membrane to 17.4 % in human bone blocks (HXB 17.4 % ± 7.4, HXB + M 25.9 % ± 10.7, BXB, 38.4 % ± 27.2, BXB + M 44.8 % ± 29.6, p = > 0.05). The use of additional membranes did not significantly affect results. Conclusions Within its limitations, results of this study suggest that solvent-preserved xenogenic human and bovine bone blocks are not suitable for lateral bone augmentation in dogs. Furthermore, defect coverage with a membrane does not positively affect the outcome.

Ability of Deproteinized Cancellous Bovine Bone to Induce New Bone Formation

2000 ◽  
Vol 71 (8) ◽  
pp. 1258-1269 ◽  
Author(s):  
Z. Schwartz ◽  
T. Weesner ◽  
S. Van Dijk ◽  
D.L. Cochran ◽  
J.T. Mellonig ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bovine Bone ◽  
New Bone Formation

scholarly journals Histometric study of socket healing after tooth extraction in rats treated with diclofenac

2002 ◽  
Vol 13 (2) ◽  
pp. 92-96 ◽  
Author(s):  
Luciana Ibara Yugoshi ◽  
Miguel Angel Sala ◽  
Luiz Guilherme Brentegani ◽  
Teresa Lúcia Lamano Carvalho
Keyword(s):  
Bone Formation ◽  
Tooth Extraction ◽  
Time Course ◽  
Volume Fraction ◽  
Blood Clot ◽  
New Bone Formation ◽  
Point Counting ◽  
Significant Delay ◽  
Socket Healing ◽  
The Right

The purpose of the present study was to investigate if diclofenac administration interferes with the time course of alveolar wound healing in rats. Forty-two Wistar rats were used, 21 rats received 10 mg/kg/day of diclofenac one day before and 4 days after extraction of the right maxillary incisors and 21 rats received saline. The animals were sacrificed 7, 14 and 21 days after tooth extraction. Progressive new bone formation and a decrease in the volume fraction of blood clot and connective tissue from 1 to 3 weeks after tooth extraction was quantified using the histometric point-counting method. Diclofenac treatment caused a significant delay in new bone formation in association with an impairment of blood clot remission/organization.

Long-Term Tissue Reactions of Three Biomaterials in Craniofacial Surgery

2007 ◽  
Vol 361-363 ◽  
pp. 1343-1346 ◽  
Author(s):  
Matti Peltola ◽  
Kalle Aitasalo ◽  
Teemu Tirri ◽  
Jami Rekola ◽  
A. Puntala
Keyword(s):  
Bone Formation ◽  
Close Contact ◽  
Healing Process ◽  
Tissue Growth ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Electron Microscopic ◽  
Composition Profiles ◽  
Tissue Reactions

Successful craniofacial reconstruction needs both a well-known and a reliable reconstruction material. However, there is often a lack of long-term knowledge of the tissue reactions and healing process in the human body. In this study, frontal sinus obliterations with bovine bone natural hydroxyapatite derivative (BHA), synthetic bioactive glass S53P4 (BAG) and hydroxyapatite cement (HAC) were investigated with clinical, histologic, scanning electron microscopic (SEM) and energy dispersive x-ray analysis (EDXA) 27, 12 and 3 years postoperatively. The aim was to determine the long-term clinical biocompatibility of the used materials. Histologic studies revealed bone formation with BHA particles and lamellar bone with BAG granule remnants in close contact to the new bone formation. In HAC reconstruction there was scattered fibroconnective tissue growth without new bone formation in the surface of HAC implantation. Neither foreign body reaction nor any abnormal findings were seen. SEM studies revealed a CaP layer on the surface of BAG granule remnants. In EDXA studies, composition profiles showed Ca-, P- and Si- rich layers on the BAG granule surface. No differences were found in CaO and P2O5 levels between BHA granules and HAC implantation and the surrounding bone. All investigated biomaterials were well tolerated in long-term applications.

Comparison of Bone Substitutes in a Tibia Defect Model in Wistar-Rats

2011 ◽  
Vol 493-494 ◽  
pp. 732-738 ◽  
Author(s):  
Cornelia Ganz ◽  
W. Xu ◽  
G. Holzhüter ◽  
W. Götz ◽  
B. Vollmar ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Substitutes ◽  
Porous Matrix ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Defect Model ◽  
Inflammatory Reactions ◽  
Nanocrystalline Hydroxyapatite ◽  
Defect Area ◽  
Tibia Defect

Various bone graft substitutes were used in clinical practise in the treatment of bone defects after trauma or osteoporosis. Many synthetic biomaterials were developed in recent years primarily based on hydroxyapatite (HA). NanoBone® is a nanocrystalline hydroxyapatite (HA) embedded in a porous matrix of silica (SiO2). The ratio of HA:SiO2 varied between 76:24 (wt%; NanoBone®) and 61:39 (wt%; Nanobone® S). The two bone substitutes NB and NB S and a natural bovine bone substitute Bio-Oss® (BO) were evaluated by means of implantation in the tibia of the rat. The aim of this study was to analyze the remodelling process and to measure new bone formation and degradation after implantation of these biomaterials. A tibia defect model was used for all investigations with testing periods of 12, 21 and 84 days. (n=5 for each time point). The results showed, that all bone grafts were well accepted by the host tissue without inflammatory reactions. In comparison to the biomaterial BO, NanoBone® and NanoBone® S were quickly degraded, whereas autologous proteins were incorporated into nanopores. New bone formation was statistically higher in NanoBone® S compared to Bio-Oss® in defect area after 84 days implantation. The presence of osteoclasts in tissue sections were demonstrated by TRAP- and ED1-immunohistology.

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