Cattleya walkeriana growth in different micropropagation systems

The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8 treatments with three repetitions. The temporary immersion bioreactor promoted a bigger growth of the aerial part and of the root system, bigger accumulation of dry mass and better control of water loss by the plants. The temporary immersion bioreactor is the best micropropagation system for the C. walkeriana growth in vitro.

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Biorreator na micropropagação de abacaxi ornamental

Ornamental Horticulture ◽

10.14295/oh.v24i2.1181 ◽

2018 ◽

Vol 24 (2) ◽

pp. 182-187

Author(s):

Caroline Oliveira Reis ◽

Adriano Bortolotti Silva ◽

Paulo Roberto Landgraf ◽

Jessica Azevedo Batisita ◽

Guilherme Antonio Jacome

Keyword(s):

Natural Ventilation ◽

Ananas Comosus ◽

Basic Medium ◽

In Vitro Cultivation ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

Time Period ◽

Culture Growth ◽

Short Time

Ornamental pineapple is a hard plant with significant landscaping value. Typically, conventional propagation is performed by clump division with low yields, and may even spread diseases. Plant tissue culture is viable, yielding plants with a high phytosanitary and genetic quality over a short time period. This study aimed to verify the in vitro multiplication of ornamental pineapple plants (Ananas comosus var. bracteatus L.) in different micropropagation systems, in association with BAP concentrations. Plants with about 2 cm were used, transplanted to the different treatments: bioreactor, natural ventilation and conventional micropropagation, combined with 3 BAP concentrations (0, 1 and 2 mg L-1). The basic medium used consisted of MS salts. The highest number of shoots and in vitro culture growth were obtained with the use of bioreactor and culture medium containing 2 mg L-1 BAP. The temporary immersion bioreactor allows air renewal inside the bottles, leading to a better performance of in vitro cultivation of ornamental pineapple, when compared to conventional micropropagation.

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Sucrose concentration and volume of liquid medium on the in vitro growth and development of blackberry cv. Tupy in temporary immersion systems

Ciência e Agrotecnologia ◽

10.1590/1413-7054201943007219 ◽

2019 ◽

Vol 43 ◽

Cited By ~ 2

Author(s):

Ricardo Antônio Ayub ◽

Jessé Neves dos Santos ◽

Luiz Antônio Zanlorensi Junior ◽

Daurimar Mendes da Silva ◽

Tereza Cristina de Carvalho ◽

...

Keyword(s):

Growth And Development ◽

Culture Medium ◽

Sucrose Concentration ◽

Seedling Production ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

Number Of Leaves ◽

Bioreactor System ◽

Temporary Immersion Systems

ABSTRACT Micropropagation of small fruits such as blackberry has been employed due to the need to obtain plants with high phytosanitary quality. Bioreactor technology has been used to improve efficiency in seedling production. Thus, the objective of this work was to evaluate the best culture medium volume and sucrose concentration for blackberry micropropagation in a temporary immersion bioreactor. In vitro blackberry shoots were segmented containing two buds and an internode (1.0 cm) and placed into MS medium supplemented with inositol (0.1 g L-1), BAP (1 mg L-1) and sucrose (10, 20, 30 or 40 g L-1) at different medium volumes (150, 175 and 200 mL). The total length, number of leaves, number of shoots, and number of hyperhydric shoots were evaluated 56 days after start of the project. For blackberry development and propagation in a bioreactor system, the best results were shown at a medium volume of 175 ml and a sucrose concentration of 20 g L-1.

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Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

3 Biotech ◽

10.1007/s13205-020-02447-3 ◽

2020 ◽

Vol 10 (10) ◽

Author(s):

Denys Matheus Santana Costa Souza ◽

Maria Lopes Martins Avelar ◽

Sérgio Bruno Fernandes ◽

Eduardo Oliveira Silva ◽

Vinícius Politi Duarte ◽

...

Keyword(s):

Eucalyptus Urophylla ◽

Spectral Quality ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

In Vitro Multiplication

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In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632021v5167131 ◽

2021 ◽

Vol 51 ◽

Author(s):

Kaliana Gottschalk de Freitas ◽

José Carlos Sorgato ◽

Jackeline Schultz Soares ◽

Luan Marlon Ribeiro

Keyword(s):

Plant Height ◽

Natural Ventilation ◽

Culture Medium ◽

Culture Media ◽

Ventilation System ◽

Light Condition ◽

Leaf Length ◽

In Vitro Growth ◽

Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

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Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452019102 ◽

2019 ◽

Vol 41 (1) ◽

Author(s):

Samila Silva Camargo ◽

Leo Rufato ◽

Maicon Magro ◽

André Luiz Kulkamp de Souza

Keyword(s):

Liquid Medium ◽

Culture Medium ◽

Culture Media ◽

Time Factors ◽

Efficient Technique ◽

Control Treatment ◽

Solid Culture ◽

Temporary Immersion ◽

Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

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Monitoring the end of the in vitro phase of Anthurium andreanum Lindl. plantlets

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202010000100007 ◽

2010 ◽

Vol 22 (1) ◽

pp. 61-68 ◽

Cited By ~ 3

Author(s):

Giulio Cesare Stancato ◽

Maria Luiza Sant’Anna Tucci

Keyword(s):

Culture Medium ◽

Soluble Sugars ◽

Dry Mass ◽

Total Soluble Sugars ◽

Gas Exchanges ◽

Single Action ◽

Fluorescent Lamps ◽

Anthurium Andreanum

Estimulation of autotrophy in in vitro plantlets could be achieved through changes in the culture medium, or by changing the traditional hermetic caps by one that could allow gas exchanges between the culture and the environment. Besides that, the use of lamps with distinct emission spectrum irradiaction has propitiated successful results. This work was carried out aiming to evaluate the either the combined or the single action of some factors that can induce autotrophy on in vitro A. andraeanum cv. Eidibel plantlets. 3 sucrose concentrations were used: 0, 15 and 60 mM and for each one, to kinds of flasks according to the cap ventilation: under (0.038 L.h-1) and without ventilation. Flasks were kept under cold light fluorescent lamps or under gro-lux lamps. At the end of the experiment showing the highest shoot dry mass treatment was 60 mM, under ventilation and gro-lux, and the treatment which accumulate root dry mass to a lesser extent were 0 mM with ventilation and cold light and 15 mM without ventilation and cold light. In average, treatments with higher sucrose content in the culture medium, that is, 60 mM, under gro-lux lamps, presented the highest chlorophyll a, b and total contents, than those under cold lamp. Steps of carbohydrates metabolism could be associated with the total soluble sugars (sucrose and reducing sugars) levels, highlighting the steps where nutrient requirements were higher, showing the role of the plantlets sink.

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Emetine and cephaeline production and regulation by in vitro propagation of Psychotria ipecacuanha (Brot.) Stokes in semi-solid media and temporary immersion bioreactor

Revista Fitos ◽

10.32712/2446-4775.2020.799 ◽

2020 ◽

Vol 14 (01) ◽

pp. 45-55

Author(s):

Simone Da Silva ◽

Danielle Cardoso Alencar ◽

Paulo José Coelho Benevides ◽

Spartaco Astolfi-Filho

Keyword(s):

Natural Populations ◽

Root Induction ◽

Relative Area ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

Mean Values ◽

Solid Media ◽

Medicinal Properties ◽

Semi Solid

Psychotria ipecacuanha, is a plant species with known medicinal properties that is critically endangered due to overexploitation of natural populations. Although the difficulties in conventional propagation by seed and by vegetative propagation are generally understood, the present study enhances our knowledge by describing efficient plant regeneration and root induction protocols for P. ipecacuanha while comparing alkaloid content (emetine and cephaeline) in in vitro-derived tissues. Stem node explants were cultured on MS medium MS supplemented with indolbutiric acid (IBA) in semi-solid media and the RITA® temporary immersion bioreactor. The highest root formation (81%) was in MS + 1.5 mg L−1 IBA in the bioreactor. After 24 months of acclimatization, the plants cultivated in MS + 0.50 and 1.0 mg L-1 of IBA had the highest number of roots (3), with mean values of 10.47 and 9.40 cm, respectively. The cultures coming from 1.0 mg L−1 and 0.5 mg L−1 IBA in the bioreactor contained higher cephaeline content, with a relative area of 14.2 and 14.9%, respectively. For emetine, the 1.0 mg L−1 IBA cultures in the bioreactor, 0.5 mg L−1 IBA and MS0 cultures contained higher content than the other treatments, with a relative area of 10.2, 10.2 and 10.1%, respectively.

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Concentrations of cytokinin and the use of agricultural residues in the in vitro propagation media of highbush blueberry

Ciência Rural ◽

10.1590/0103-8478cr20190153 ◽

2020 ◽

Vol 50 (6) ◽

Author(s):

Michele Carla Nadal ◽

Adriane Marinho de Assis ◽

Dianini Brum Frölech ◽

Bruna Andressa dos Santos Oliveira ◽

Cari Rejane Fiss Timm ◽

...

Keyword(s):

Rice Husk ◽

In Vitro Propagation ◽

Culture Medium ◽

Propagation Rate ◽

Dry Mass ◽

Vaccinium Corymbosum ◽

Agricultural Residues ◽

Highbush Blueberry ◽

Rice Husks

ABSTRACT: The objective of the research was to evaluate cytokinin concentrations and agricultural residues as physical structuring agents of the culture medium in the in vitro propagation of highbush blueberry (Vaccinium corymbosum ‘Duke’). To that end, three different concentrations of 2-isopentenyladenine (2iP) (0, 5, and 10 mg L-1)in woody plant medium (WPM) and five structuring agents (in natura rice husks, carbonized rice husks, coconut fiber, S-10 Beifort®, and agar)were tested. After 60days of culture, the following parameters were evaluated: survival (%),sprout and shoot lengths (cm),propagation rate, number of sprouts, and shoot dry mass (g). The substrates used as structuring agents were analyzed in terms of pH and electrical conductivity. The in natura rice husk, S-10 Beifort®, and carbonized rice husk did not differ from the agar in terms of the sprout length. The shoots were longer (1.64 cm) in cultures within natura rice husk than in other treatments. In the absence of 2iP, S-10 Beifort® resulted in the highest propagation rate (2.97). Concerning the number of sprouts, S-10 Beifort®, in natura rice husks, and carbonized rice husks did not differ from agar. However, when the regulator was added, the agar performed better in comparison to the other residues. Thus, in natura rice husks, carbonized rice husks, and S-10 Beifort®are potential physical structuring agents of the culture medium that can be used without 2iP.

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Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

Journal of Agricultural Studies ◽

10.5296/jas.v8i2.16863 ◽

2020 ◽

Vol 8 (2) ◽

pp. 774

Author(s):

Nastassja Kimberlly Lima ◽

Eloisa Schneider Da Silva ◽

Rayane Monique Sete Da Cruz ◽

Pedro Henrique Riboldi Monteiro ◽

Glacy Jaqueline Da Silva

Keyword(s):

Growth Regulators ◽

Root Length ◽

Aerial Part ◽

Large Scale ◽

Dry Mass ◽

Fresh Mass ◽

Limiting Factor ◽

Naphthalene Acetic Acid ◽

In Vitro Cultivation

Acmella oleracea is a tropical plant, typical of the northern region of Brazil. The species belongs to the Asteraceae family and has great therapeutic, pharmacological and industrial potential. A limiting factor for the production of this species on a large scale is the short life cycle. The tissue culture programs use synthetic hormones based on cytokinins, such as kinetin and benzylaminopurine (BAP) and auxins such as naphthalene acetic acid (ANA). The objective of this research was to evaluate the effect of growth regulators on the production of Acmella oleracea "in vitro". The experimental test was carried out with control (C), without the addition of growth regulators and five treatments, composed of: (T1) 0.1; (T2) 0.3; (T3) 0.5 mg L-1 kinetin; (T4) 0.1 mg L-1 of BAP and ANA; (T5) 0.5 mg L-1 of BAP and ANA. The experimental design was a completely randomized block in a factorial arrangement with six treatments, three blocks and twenty-five repetitions per block. The evaluated parameters were: germination, root formation, aerial part length, root length, aerial part fresh mass and root fresh mass, aerial part dry mass and root dry mass. The data obtained were subjected to analysis of variance (p <0.05) and compared using the Tukey test. The results showed that kinetin positively contributed to seed germination and aerial part dry mass development. Treatment 1 had the best results for the parameters root length, shoot length and root dry mass.

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Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

HortScience ◽

10.21273/hortsci.45.7.1093 ◽

2010 ◽

Vol 45 (7) ◽

pp. 1093-1098 ◽

Cited By ~ 9

Author(s):

Jericó J. Bello-Bello ◽

Adriana Canto-Flick ◽

Eduardo Balam-Uc ◽

Eunice Gómez-Uc ◽

Manuel L. Robert ◽

...

Keyword(s):

Shoot Elongation ◽

Nodal Segments ◽

High Survival ◽

Shoot Induction ◽

Capsicum Chinense ◽

Temporary Immersion Bioreactor ◽

In Vitro Proliferation ◽

Temporary Immersion ◽

Habanero Pepper

This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated containers, the age of the explant donor plants as well as the effect of thidiazuron and paclobutrazol on shoot induction and of gibberellic acid and AgNO3 on shoot elongation were also evaluated. A temporary immersion bioreactor (BioMINT™) was used for the multiplication and elongation of isolated shoots with very good results. We report an efficient protocol for the in vitro propagation of Habanero pepper that produces plants with a high survival rate when transplanted to soil.

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