Pretreatment with H2O2 in maize seeds: effects on germination and seedling acclimation to salt stress

RESUMO - Durante o processo de produção de sementes, estresses abióticos como salinidade do solo e déficit hídrico são fatores determinantes e que influenciam no estabelecimento e desenvolvimento uniforme do estande em campo. Nesse sentido, cultivares tolerantes a altas concentrações salinas nestas fases são fundamentais nos programas de melhoramento. Diante disto, objetivou-se com a presente pesquisa verificar a influência da produção de sementes sob condições de estresse hídrico e salino na germinação e no vigor, assim como na atividade da enzima α-amilase em sementes de milho. Para isso, foram conduzidos dois experimentos para a produção de sementes de milho, um em condições de estresse salino e o outro sob condições de déficit hídrico. Os experimentos foram conduzidos em delineamento de blocos completos casualisados, em esquema de parcelas subdivididas. As sementes colhidas em diferentes estádios de desenvolvimento, determinados pela linha de leite (LL), foram submetidas a análises fisiológicas e de vigor, bem como determinou-se a expressão da enzima α-amilase por eletroforese. Diante dos resultados foi possível concluir que a germinação e o vigor das sementes produzidas em solos com condutividade de 3 dS m-1 não são afetados pela salinidade, e o desenvolvimento das plantas em condições de estresse hídrico e salino não afetou a atividade da enzima α-amilase em sementes colhidas na LL5. Palavras-chave: Zea mays L., eletroforese, estádios de desenvolvimento. PHYSIOLOGICAL QUALITY AND ALPHA-AMYLASE EXPRESSION OF MAIZE SEEDS IN CONDITIONS OF SALT AND WATER STRESS ABSTRACT - During seed production process, abiotic stresses such as soil salinity and drought are determining factors that influence the establishment and development of uniform stand on the field. Thus, tolerant cultivars to high salt concentrations in these phases are critical in breeding programs. This way, we aimed to investigate seeds production in conditions of water and salt stress on maize seeds germination and vigor as well as the α-amylase enzyme activity. Two experiments were performed for maize seeds production in salt stress conditions and drought. The experiments were conducted in a randomized complete block design in a split-plot arrangement. Seeds harveste at different development stages, determined by milk line (ML), were submitted to vigor and physiological analysis as well as the expression determination of α-amylase enzyme by electrophoreses. With the results it was concluded that both, the seeds germination and vigor produced in soil with conductivity 3 dS m-1, are not affected by salinity, and the plants development in water and salt stress conditions did not affect the activity of α-amylase enzyme in seedsharvested in ML5 maturity stage. Keywords: Zea mays L., electrophoresis, development stages.

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Exogenous mammalian sex hormones mitigate inhibition in growth by enhancing antioxidant activity and synthesis reactions in germinating maize seeds under salt stress

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.4655 ◽

2011 ◽

Vol 92 (4) ◽

pp. 839-843 ◽

Cited By ~ 23

Author(s):

Serkan Erdal

Keyword(s):

Antioxidant Activity ◽

Salt Stress ◽

Sex Hormones ◽

Maize Seeds ◽

Synthesis Reactions

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Salicylic acid: physiological and biochemical changes in seeds and maize seedlings subjected to salt stress

Journal of Seed Science ◽

10.1590/s2317-15372013000400007 ◽

2013 ◽

Vol 35 (4) ◽

pp. 457-465 ◽

Cited By ~ 1

Author(s):

Fernanda Reolon Tonel ◽

Patrícia Marini ◽

Juliana de Magalhães Bandeira ◽

Dario Munt de Moraes ◽

Luciano do Amarante

Keyword(s):

Salicylic Acid ◽

Antioxidant Enzymes ◽

Salt Stress ◽

Seed Viability ◽

Initial Growth ◽

Maize Seedlings ◽

Maize Seeds ◽

Nacl Concentration ◽

Physiological And Biochemical ◽

Defense Function

Several plant species have development limitations on saline soils; however under water stress situations salicylic acid (SA) may have defense function by acting as antioxidant. Thereby, this study was aimed at analyzing physiological changes occurring in maize seeds treated with SA and germinated on sodium chloride moistened substrate; as well as assessing the effect of antioxidant enzymes produced by the seedlings. To this, seeds were soaked into zero and 1 mM SA solutions, for 60 min., and germinated on different NaCl concentrations (0, 30, 90, and 180 mM), and afterwards evaluated for germination, germination speed index, initial growth, and effect of antioxidant enzymes on seedlings. For electrical conductivity test, the seeds were previously soaked in NaCl solutions with and without SA. The SA application has minimized damages induced by salt stress to maize seed germination by favoring their germination percent; however without SA was not able on maintaining seed viability over 90 mM. Seedling initial growth was impaired by increasing NaCl concentrations in substrate; and electric conductivity has shown low values for release of solutes in the presence of SA. Activity of antioxidant enzymes has increased until 30 mM NaCl concentration; however above such concentration activity of these enzymes was reduced. The SA maintains germination of corn seeds above 90 mM NaCl; however, generally it is not efficient to ensure vigor of seedlings.

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Vigour in maize seeds: influence on seedling development under conditions of salt stress

Revista Ciência Agronômica ◽

10.5935/1806-6690.20160059 ◽

2016 ◽

Vol 47 (3) ◽

pp. 491-499 ◽

Cited By ~ 1

Author(s):

Rosemeire Carvalho da Silva ◽

Camila Ribeiro de Souza Grzybowski ◽

Maristela Panobianco

Keyword(s):

Salt Stress ◽

Seedling Development ◽

Maize Seeds

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Effects of vitamin pre-sowing treatment on sweet maize seedlings irrigated with saline water

Acta Agronómica ◽

10.15446/acag.v69n1.67528 ◽

2020 ◽

Vol 69 (1) ◽

pp. 20-25

Author(s):

Eduardo Pradi Vendruscolo ◽

Alexsander Seleguini

Keyword(s):

Salt Stress ◽

Salinity Stress ◽

Dry Matter ◽

Saline Water ◽

Initial Growth ◽

Initial Development ◽

Combined Application ◽

Maize Seeds ◽

Negative Effect ◽

Combined Solution

Salinity stress represents an obstacle for the production of plants of commercial interest, including sweet maize. Among the techniques used to suppress the effects caused by excess salts in the soil is the exogenous application of vitamins. Thus, the objective of this study was to evaluate the effect of the pre-sowing treatment of sweet maize seeds in solutions of thiamine, niacin and these two vitamins combined on the development of the plants irrigated with saline water. The treatments were composed by the 24 h pre-sowing treatment with water, thiamine solution (thiamine 100 mg L-1), niacin solution (niacin 100 mg L-1) and a combined solution with these two vitamins (thiamine 50 mg L-1 + niacin 50 mg L-1) irrigated with saline water. A control (pre-sowing treatment with water and irrigation with non-saline water) was also used. It was observed that salinity stress affects the sweet maize initial development. Application of isolate thiamine or niacin and its combined application improves the relative contents of chlorophyll ‘a’ and total, height of shoot, leaf number, diameter of stem and shoot, root and total dry matter, reducing dry matter losses by 8.89%, 25.46% and 39.60%, respectively. Soaking seeds for 24 h in thiamine and niacin solutions improve the initial growth of sweet maize plants under salt stress and a combined vitamin solution (thiamine 50 mg L-1 + niacin 50 mg L-1) effectively reduces the salt stress negative effect on the initial development of these plants.

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Mitigation of adverse effects of salt stress on germination, growth, photosynthetic efficiency and yield in maize (Zea mays L.) through magnetopriming

Acta Agrobotanica ◽

10.5586/aa.1757 ◽

2019 ◽

Vol 72 (1) ◽

Cited By ~ 8

Author(s):

Lokesh Baghel ◽

Sunita Kataria ◽

Meeta Jain

Keyword(s):

Salt Stress ◽

Growth Parameters ◽

Photosynthetic Performance ◽

Physiological Characteristic ◽

Tolerance Index ◽

Seedling Vigor ◽

Maize Seeds ◽

Psii Photochemistry ◽

Maize Plants ◽

The Impact

The efficiency of magnetopriming was evaluated for mitigation of the detrimental effects of salt stress on maize germination, growth, photosynthesis, and yield of maize plants. Maize seeds were pretreated with 200 mT of static magnetic field (SMF) for 1 h to assess the impact of SMF on the germination, seedling vigor, growth of plant, photosynthetic performance, ROS content, and yield under salt stress. The seedling characteristics of maize were negatively influenced by salt stress. However, SMF-pretreated maize seeds showed relatively higher germination percentage and germination stress tolerance index as compared to untreated seeds in saline and nonsaline conditions. The detrimental effect of NaCl induced salt stress was also observed on growth, yield, and different physiological characteristic of maize plants. The results showed that SMF-pretreated seeds enhanced seedling vigor, growth parameters such as plant height, leaf area, and biomass accumulation at different concentrations of NaCl (0, 25, 50, 75, and 100 mM) as compared to untreated seeds. Photosynthetic pigments, quantum yield of PSII photochemistry (Fv/Fm), phenomenological fluxes such as electron transport per leaf CS (ETo/CSm) and density of reaction centers (RC/CSm), the performance index (PI) were high in the leaves of plants that emerged from SMF-pretreated seeds as compared to untreated seeds. This stimulatory effect of SMF treatment of seeds was also revealed in the rate of photosynthesis and stomatal conductance, which results in improved yield of maize plants under saline conditions. The leaves from plants of SMF-treated seeds showed decreased hydrogen peroxide (H2O2) when compared with untreated seeds in both conditions. SMF ameliorates the adverse effect of salt stress in maize plants, by reducing H2O2 and increasing growth, photosynthetic performance, and yield under salt stress. For improvement of salt tolerance, magnetopriming with SMF of 200 mT for 1 h to dry seeds of maize can be efficiently used as a presowing treatment.

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