scholarly journals Susceptibility of Gyropsylla spegazziniana (Lizer & Trelles) (Hemiptera: Psyllidae) to Beauveria bassiana (Bals.) Vuill.

2013 ◽  
Vol 80 (3) ◽  
pp. 363-366 ◽  
Author(s):  
Luis Francisco Angeli Alves ◽  
Marina Andressa Formentini ◽  
André Luis Pereira Fanti ◽  
Maria Elena Schapovaloff ◽  
Ionete Lúcia Milani Barzotto

Gyropsylla spegazziniana is one of the most prominent pest insects of yerba mate culture in all production regions in Brazil, Argentina and Paraguay. Natural enemies have been recorded on G. spegazziniana nymphs and adults, including the Zoophthora radicans entomopathogenic fungus. Since there are no reports of Beauveria bassiana, the aim of this study was to register its pathogenicity with respect to this insect in a laboratory setting. Yerba mate branches were kept in glass flasks with water and we infested each leaf with 20 newly-emerged nymphs. We prepared three replicates per treatment. We sprayed conidia suspensions (1 × 109 conidia/mL) onto the branches, which we transferred to cages and kept in an acclimatized room (26 ± 1°C, 70 ± 10% of relative humidity (R.H.) with a photophase of 14 hours). We evaluated insect mortality daily, and after five days we observed a high level of pathogenicity due to the presence of the fungus, that could be visually observed on the cadaver. The total mortality varied from 25 to 70% (respectively for Unioeste 4 and CG 716) and the confirmed mortality was 30% for Unioeste 52, revealing the fungus' potential. However, more studies are necessary in order to evaluate the strains of this fungus, as well as other species.

Agrivet ◽  
2019 ◽  
Vol 25 (2) ◽  
pp. 124
Author(s):  
Chimayatus Solichah ◽  
Anindita Widyaningtyas ◽  
Rukmowati Brotodjojo

Coffee is a commodity with high value for export. Coffee bean borer (Hypothenemus hampei) is the main pest causing the decline in coffee production. Farmers still rely on chemical insecticides to control this pest. To reduce the negative impact of insecticides, entomopathogen Beauveria bassiana is applied. The research aimed to investigating the level of pathogenicity of B. bassiana in controlling H. hampei, and investigating the concentration with the highest level of pathogenicity of B. Bassiana against H. hampei. Laboratory research was arranged in one factor Completely Random Design (CRD). This research used 8 (eight) treatments namely KO: Aquades, Kk: Lamda Sihalothrin 25 EC (Chemical Insecticide), B1: 10 g / L B. bassiana cultivated in rice bran media, B2: 20 g / L B. bassiana cultivated in rice bran media, B3: 30 g / L B. bassiana cultivated in rice bran media, B4: 10 g / L B. bassiana grown on corn media, B5: 20 gL B. bassiana grown on corn and B6: 30 g / L B. bassiana grown on corn media. Each treatment used coffee beans infested with 20 H. hampei and repeated 4 (four) times. The result of the research showed that B. bassiana was able to kill H. hampei. The concentration that was able to cause the fastest mortality with a high level of pathogenicity based on the pathogenicity test of B. bassiana on H. hampei (percentage of mortality, total mortality time, and feeding capacity) was 30 g/L of B. bassiana cultivated in rice bran media.Key words: Beauveria bassiana, Hypothenemus hampei, chemical insecticide, coffee bean


FLORESTA ◽  
2011 ◽  
Vol 41 (1) ◽  
Author(s):  
Larissa Rolim Borges ◽  
Sônia Maria Noemberg Lazzari ◽  
Ida Chapaval Pimentel ◽  
Lívia Cardoso Bueno de Camargo ◽  
Lílian Mattos Kalinoski

O controle da broca-da-erva-mate, Hedypathes betulinus, limita-se ao emprego de práticas silviculturais e catação manual dos insetos adultos, não sendo permitido o uso de inseticidas químicos na cultura. Esta pesquisa teve por objetivos verificar a presença natural de Beauveria bassiana no solo de plantio em monocultura de erva-mate, determinar a persistência de B. bassiana no solo após duas e três aplicações do fungo e averiguar a influência de fatores abióticos na estabilidade do fungo no solo. O estudo foi conduzido em um erval no município de Campo Alegre, Santa Catarina, Brasil, onde foram coletadas amostras de solo em duas áreas, para avaliação da presença natural de inóculos do fungo B. bassiana, procedendo-se à contagem, isolamento e identificação.Não foi detectada a ocorrência natural do fungo no solo em nenhuma das duas áreas. Após essa confirmação, foram realizadas pulverizações com B. bassiana em ambas as áreas, sendo que em uma ocorreram duas e na outra três aplicações. Após a última pulverização, realizada em fevereiro/2006, verificou-se que os inóculos do fungo persistiram por 90 dias na área com duas aplicações e 120 dias na área com três aplicações. A temperatura afetou a persistência do fungo no solo em ambas as áreas, mas a umidade relativa não mostrou influência sobre os inóculos em campo.Palavras-chave: Broca-da-erva-mate; controle biológico; epizootia; fungo entomopatogênico. AbstractPersistence in soil of inoculum of Beauveria bassiana used to control Hedypathes betulinus in yerba maté plantation, Ilex paraguariensis. Control measures of Hedypathes betulinus are restricted to agricultural practices and hand picking of adult insects. No chemical control is allowed in yerba maté cultivation areas. The objectives of this research were to verify the natural occurrence of the entomopathogenic fungus Beauveria bassiana in the soil of yerba maté in monoculture; to determine the persistence of inoculums in the soil after applications of the fungus; and to evaluate the influence of abiotic factors on fungus stability in the soil. The soil samples were taken from two experimental areas in a yerba maté plantation in the county of Campo Alegre, Santa Catarina, Brazil. The samples were analyzed in order to count, isolate and identify inoculums of B. bassiana and other fungi. After the confirmation that B. bassiana was not present in the soil, a suspension of the fungus was applied, twice in one of the areas and three times in the other. After the last application in February/2006, the inoculum persisted for 90 and 120 days, in the area with two and three applications, respectively. The temperature affected the persistence of B. bassiana in soil; however, the relative humidity did not.Keywords: Biological control; epizooty; entomopathogenic fungus; wood borer. 


Author(s):  
S. Pal ◽  
S. Samanta ◽  
A. Banerjee

Background: Field pea, Pisum sativum L. is an important winter-season pulse crop. It is subjected to damage by both field and storage insect pests and approximately 10-15 per cent reduction in yield was reported due to the infestation of different insect pests. Among these, pulse aphid (Aphis craccivora Koch.) affects plant physiology directly by removal of nutrients or indirectly by dispersal of various viral diseases. The present investigation has been aimed to study the seasonal fluctuations of aphids and their natural enemies as well as their correlation. Another objective was to know the effect of various weather parameters on pulse aphids and their natural enemies which ultimately would be helpful to develop a forewarning model.Methods: The field experiment was conducted at the A-B Block Farm of Bidhan Chandra Krishi Viswavidyalaya, Nadia, West Bengal using two varieties of field pea (KPMR 935 and IFPD 122) during rabi seasons of two consecutive years of 2017-18 and 2018-19 following a Randomized Block Design with three replications. After recording the total population of both pests (nymphs and adults) and their natural enemies across the season, the mean population was worked out and used for correlation and regression studies along with the weather parameters. Result: Maximum aphid population was noticed during the peak pod formation stage of the crop irrespective of the varieties. The pest population was very strongly correlated with the incidence of coccinellid and ant population in both test varieties. Among the weather parameters, both maximum and minimum temperature and sunshine hour showed a positive correlation with the pest population and their natural enemies but relative humidity and rainfall showed a negative correlation. Regression studies indicated that temperature and relative humidity were the most influencing factors over the incidence of aphid in both the seasons.


Author(s):  
M. Estrada ◽  
Manuel Camacho ◽  
César Benito

AbstractInter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.


2019 ◽  
Vol 8 (1) ◽  
pp. 31-38
Author(s):  
Khoirul Ikhsanudin Hanif ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Tili Karenina ◽  
Erise Anggraini ◽  
...  

Hanif et al, 2019. Population of Pest Insects and Predatory Arthropods Inhabiting Freshwater Swamp Rice of South Sumatra Treated with Bioinsectide of Beauveria bassiana and a Synthetic Insecticide. JLSO (8)1:31-38.Entomopathogenic fungi, Beauveria bassiana, could  kill various species of insect pests, but  their impact on predatory arthropods needs to be studied. The aim of this study was to compare population of pest insects and predatory arthropods inhabiting rice sprayed with bioinsecticide of B. bassiana and conventional rice field using a synthetic insecticides. Bioinsecticide was made from conidia of B. bassiana and sprayed on rice canopy. The plot of conventional farmers was sprayed with synthetic insecticides made from Abamektrin. The results showed that the application of bioinsecticide of B. bassiana was not proven to reduce the pest insect population. The abundance of natural enemies of pest insects, such as  spiders and predatory insects was also more abundant in plots sprayed with  bioinsecticide than conventional plots that used synthetic insecticide. The synthetic insecticides have been shown to reduce the abundance of predatory insects and spiders. Bioinsecticides containing carrier of  liquid compost were proven to increase the growth and development of rice, which was an increase in the number of rice tillers per clump in plots applied by bioinsecticide. Thus, B. bassiana of bioinsecticide with liquid compost carrier material was safer and could maintain the abundance of predatory arthropods and increased rice growth.


2019 ◽  
Vol 7 ◽  
pp. 14
Author(s):  
Sandra Rodas ◽  
Pedro Acuña

La Yerba Mate (Ilex paraguariensis St. Hil), es un rubro tradicional del Paraguay desarrollado al inicio de forma extractiva de los bosques y con el fin de aumentar la productividad se alteró su hábitat natural y con ello también la incidencia de insectos que se mantenían en equilibrio originalmente; dándose una especial atención a la Gyropsylla spegazziniana, agente causal del rulo de la Yerba Mate. Al no existir un método de control alternativo para esta plaga, se llevó a cabo este trabajo.


2018 ◽  
Vol 60 (3) ◽  
pp. 161-172 ◽  
Author(s):  
Iwona Skrzecz ◽  
Aldona Perlińska

Abstract Current problems of forest protection concern the declining health of forest stands due to climate change and the resulting extreme weather events such as heat waves, droughts, hurricane winds, heavy rainfalls and floods. Repeated impacts of these factors increase susceptibility of forest stands to pest insects and fungal pathogens. Norway spruce (Picea abies [L.] H. Karst) is sensitive to high air temperatures and water shortage. Long lasting droughts during the last two decades, have been one of the reasons behind Norway spruce dieback due to severe outbreak of European spruce bark beetle Ips typographus (L.) in the mountainous regions of southern Poland. In the Scots pine (Pinus sylvestris L.) stands, water balance disorders have enhanced the colonization of weekend trees by steelblue jewel beetle Phaenops cyanea (F.) and engraver beetle Ips acuminatus (Gyll.), as well as contributed to the spread of fungal diseases caused by Gremmeniella abietina (Lagerb.) Cenangium ferruginosum Fr. and Sphaeropsis sapinea Fr. fungi. Water related stress leads to weakening of oak stands, which are attacked by Agrilus spp. beetles and pathogens from the genus Phytophthora. It is possible that long lasting droughts initiated the spread of infectious ash disease caused by Hymenoscyphus fraxineus (= Chalara fraxinea), which resulted in the epidemic of ash dieback throughout Europe. Until recently, the use of plant protection products was the most common method of forest protection against pest insects and pathogens. Poland’s accession to the European Union has affected the marketing and use of plant protection products in the country. The implementation of the EU legislation (Directive 91/414/EEC, Directive 2009/128/ EC and Regulation No 1107/2009) has resulted in decreased assortment of pesticides registered for the protection of forests. High costs and long registration process considerably limited the interest of producers in placing the plant protection products on the market. Systematic decrease in the number of plant protection products possible to register for use in forestry, as well as the principles of integrated plant protection established in the EU in 2014 call for seeking plant protection methods based on the natural enemies of pests, such as pathogenic microorganisms, parasites and predators. Therefore, contemporary forest protection requires the advancement of integrated methods for pest insect and disease control through developing methods of forecasting forest dangers, the use of natural enemies and agro-technical methods for regulation of pests, as well as the development of decision support systems as a tool facilitating introduction of integrated forest protection principles. Such support systems help to establish optimal terms for the implementation of protection measures, so as to increase their efficiency while limiting the use of chemical pesticides to an absolute minimum.


2019 ◽  
Vol 92 (3) ◽  
pp. 1271-1281 ◽  
Author(s):  
N. González-Mas ◽  
M. Cuenca-Medina ◽  
F. Gutiérrez-Sánchez ◽  
E. Quesada-Moraga

2008 ◽  
Vol 53 (Special Issue) ◽  
pp. 53-56
Author(s):  
O. Nakládal ◽  
M. Turčáni

Predation by natural enemies was studied as mortality factors of <I>Hylesinus fraxini</I> in 2006–2007. Predation by birds was not recorded and predation rate by insect reached 0.1%. Only one insect predator was reared – <I>Thanasimus formicarius</I> (Linnaeus, 1758). The total rate of parasitism was 54.0%. At least 8 species of parasitoids from the order Hymenoptera were recorded. The most abundant parasitoid was <I>Eurytoma morio Boheman</I>, 1836 (44.1% from the total number of all reared parasitoids; 23.8% of the total mortality), subsequently <I>Eurytoma arctica</I> Thomson, 1876 (31.7%; 17.1%), <I>Cheiropachus quadrum</I> (Fabricius, 1787) (12.8%; 6.9%), Braconidae (6.3%; 3.4%), <I>Rhaphitelus maculatus</I> Walker, 1834 (3.9%; 2.1%), <I>Mesopolobus</I> sp. (0.5%; 0.3%), were not identified Pteromalidae (0.5%; 0.3%) and <I>Tetrastichus</I> sp. (0.2%; 0.1%).


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