scholarly journals PATHOGENICITY TEST OF VARIOUS CONCENTRATIONS OF Beauveria bassiana CULTURED ON RICE BRAN AND CORN MEDIA AGAINST COFFEE FRUITS BORER (Hyphotenemus hampei)

Agrivet ◽  
2019 ◽  
Vol 25 (2) ◽  
pp. 124
Author(s):  
Chimayatus Solichah ◽  
Anindita Widyaningtyas ◽  
Rukmowati Brotodjojo

Coffee is a commodity with high value for export. Coffee bean borer (Hypothenemus hampei) is the main pest causing the decline in coffee production. Farmers still rely on chemical insecticides to control this pest. To reduce the negative impact of insecticides, entomopathogen Beauveria bassiana is applied. The research aimed to investigating the level of pathogenicity of B. bassiana in controlling H. hampei, and investigating the concentration with the highest level of pathogenicity of B. Bassiana against H. hampei. Laboratory research was arranged in one factor Completely Random Design (CRD). This research used 8 (eight) treatments namely KO: Aquades, Kk: Lamda Sihalothrin 25 EC (Chemical Insecticide), B1: 10 g / L B. bassiana cultivated in rice bran media, B2: 20 g / L B. bassiana cultivated in rice bran media, B3: 30 g / L B. bassiana cultivated in rice bran media, B4: 10 g / L B. bassiana grown on corn media, B5: 20 gL B. bassiana grown on corn and B6: 30 g / L B. bassiana grown on corn media. Each treatment used coffee beans infested with 20 H. hampei and repeated 4 (four) times. The result of the research showed that B. bassiana was able to kill H. hampei. The concentration that was able to cause the fastest mortality with a high level of pathogenicity based on the pathogenicity test of B. bassiana on H. hampei (percentage of mortality, total mortality time, and feeding capacity) was 30 g/L of B. bassiana cultivated in rice bran media.Key words: Beauveria bassiana, Hypothenemus hampei, chemical insecticide, coffee bean

Author(s):  
R.R. Rukmowati Brotodjojo ◽  
Chimayatus Solichah ◽  
Anindita Widyaningtyas ◽  
Danar Wicaksono

Coffee bean borer (Hypothenemus hampei) attacks coffee when it is still in the plantation until it is in the storage that reduce the quality of coffee. To avoid the negative impact of chemical insecticides, biological control is carried out by using entomopathogen Beauveria bassiana. The aims of this research were to study the effects of culture media on viability of B. bassiana and its pathogenicity in controlling H. hampei. Laboratory experiment was arranged in Completely Random Design (CRD). This research used 8 (eight) treatments namely KO: Distilled water, Kk: Lamda Sihalothrin 25 EC (Chemical Insecticide), B1: 10 g/L B. bassiana cultured in rice bran media, B2: 20 g/L B. bassiana cultured in rice bran media, B3: 30 g/L B. bassiana cultured in rice bran media, B4: 10 g/L B. bassiana cultured in corn media, B5: 20 g/L B. bassiana cultured in corn media and B6: 30 g/L B. bassiana cultured in corn media. Each treatment was applied on coffee beans infested with 20 H. hampei and repeated 4 (four) times. The results showed that rice bran media produced the highest number of spores with highest viability. The application of B. bassiana could killed H. hampei. The concentration of 30 g/L of B. bassiana cultured in rice bran media resulted in the fastest mortality (Mortality time and Lethal Time 50%) of H. hampei.


2013 ◽  
Vol 80 (3) ◽  
pp. 363-366 ◽  
Author(s):  
Luis Francisco Angeli Alves ◽  
Marina Andressa Formentini ◽  
André Luis Pereira Fanti ◽  
Maria Elena Schapovaloff ◽  
Ionete Lúcia Milani Barzotto

Gyropsylla spegazziniana is one of the most prominent pest insects of yerba mate culture in all production regions in Brazil, Argentina and Paraguay. Natural enemies have been recorded on G. spegazziniana nymphs and adults, including the Zoophthora radicans entomopathogenic fungus. Since there are no reports of Beauveria bassiana, the aim of this study was to register its pathogenicity with respect to this insect in a laboratory setting. Yerba mate branches were kept in glass flasks with water and we infested each leaf with 20 newly-emerged nymphs. We prepared three replicates per treatment. We sprayed conidia suspensions (1 × 109 conidia/mL) onto the branches, which we transferred to cages and kept in an acclimatized room (26 ± 1°C, 70 ± 10% of relative humidity (R.H.) with a photophase of 14 hours). We evaluated insect mortality daily, and after five days we observed a high level of pathogenicity due to the presence of the fungus, that could be visually observed on the cadaver. The total mortality varied from 25 to 70% (respectively for Unioeste 4 and CG 716) and the confirmed mortality was 30% for Unioeste 52, revealing the fungus' potential. However, more studies are necessary in order to evaluate the strains of this fungus, as well as other species.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Verónica Gamón ◽  
Isabel Hurtado ◽  
José Salazar-Fraile ◽  
Gabriel Sanfélix-Gimeno

AbstractSchizophrenia is a chronic mental condition presenting a wide range of symptoms. Although it has a low prevalence compared to other mental conditions, it has a negative impact on social and occupational functions. This study aimed to assess the appropriateness of antipsychotic medications administered to schizophrenic patients and describe current treatment patterns for schizophrenia. A retrospective cohort study was conducted in all patients over the age of 15 with an active diagnosis of schizophrenia and treated with antipsychotics between 2008 and 2013 in the Valencia region. A total of 19,718 patients were eligible for inclusion. The main outcome assessed was inappropriateness of the pharmacotherapeutic management, including polypharmacy use. Altogether, 30.4% of patients received antipsychotic polypharmacy, and 6.8% were prescribed three or more antipsychotics. Overdosage affected 318 individuals (1.6%), and 21.5% used concomitant psychotropics without an associated psychiatric diagnosis. Women and people with a comorbid condition like anxiety or depression were less likely to receive antipsychotic polypharmacy. In contrast, increased polypharmacy was associated with concomitant treatment with other psychoactive drugs, and only in user on maintenance therapy, with more visits to the mental health hospital. Overall, we observed a high level of inappropriateness in antipsychotic prescriptions. Greater adherence to guidelines could maximize the benefits of antipsychotic medications while minimizing risk of adverse effects.


Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 518
Author(s):  
Bronwyn Egan ◽  
Zwannda Nethavhani ◽  
Barbara van Asch

Macrotermes termites play important ecological roles and are consumed by many communities as a delicacy and dietary complement throughout Africa. However, lack of reliable morphological characters has hampered studies of Macrotermes diversity in a wide range of scientific fields including ecology, phylogenetics and food science. In order to place our preliminary assessment of the diversity of Macrotermes in South Africa in context, we analysed a comprehensive dataset of COI sequences for African species including new and publicly available data. Phylogenetic reconstruction and estimates of genetic divergence showed a high level of incongruity between species names and genetic groups, as well as several instances of cryptic diversity. We identified three main clades and 17 genetic groups in the dataset. We propose that this structure be used as a background for future surveys of Macrotermes diversity in Africa, thus mitigating the negative impact of the present taxonomic uncertainties in the genus. The new specimens collected in Limpopo fell into four distinct genetic groups, suggesting that the region harbours remarkable Macrotermes diversity relative to other African regions surveyed in previous studies. This work shows that African Macrotermes have been understudied across the continent, and that the genus contains cryptic diversity undetectable by classic taxonomy. Furthermore, these results may inform future taxonomic revisions in Macrotermes, thus contributing to advances in termitology.


2003 ◽  
Vol 9 (2) ◽  
pp. 170-175 ◽  
Author(s):  
Michitaka NAITO ◽  
Xiaohong WU ◽  
Jer-Min LIN ◽  
Akihiko KIMURA ◽  
Michiteru KODAMA ◽  
...  
Keyword(s):  

2021 ◽  
Vol 72 (5) ◽  
pp. 110-118
Author(s):  
M. Shkurat ◽  
K. Pavlotska

Analysis of the migration process of the Ukrainian population, which takes place within the state and at the international level during 2014–2019 is carried out in this paper. On the basis of scientific works of domestic scientists and institutes the problems which have not been investigated, and also aspects which have been solved partially are defined. The negative consequences of the Ukrainian migration process on the state of the country are identified. The classification of migration flows of the Ukrainian population according to the reasons of movement is carried out. The main regions and countries which, as of the beginning of 2021, are more attractive to compatriots and are characterized by significant influxes of Ukrainians are identified. The main centers of departure – regions and countries – which are characterized by high level of outflow of Ukrainians are determined, the main problems and reasons that motivate the outflow of Ukrainians are highlighted in this paper. The level of growth of the Ukrainian population in terms of migration flows is defined. The investigation of Ukrainian diasporas, territorial location and analysis of their share in comparison is carried out. The main factors that force the Ukrainian population to move, which affects the demographic situation in Ukraine, the level of employment, as well as the amount of cash flows to the country and the state of the economy as a whole are identified. The main regulations and identified solutions to the migration flows of the domestic population, which were approved and implemented in the period from 2001 to 2019 are compared in this paper. On the basis of current and newly introduced strategies, critical analysis of the ways of regulating the migration process of the Ukrainian population is carried out, the main aspects and problems of the implemented migration policy of Ukraine are determined. The results of the work are to identify the main ways to reduce the negative impact of the migration process on the country's economy and related processes, and strategies to benefit from the movement of citizens of Ukraine, namely: introduction of intellectual security, revision of wages by region, stimulating the process of attracting technology in all spheres of activity and formation of the social protection system.


2021 ◽  
pp. 133-140
Author(s):  
L. I. ATKINA ◽  
◽  
L. V. BULATOVA ◽  
L. P. ABRAMOVA

Based on a comprehensive assessment of the state of the park landscape, the level of its anthropogenic transformation is determined, which is necessary to identify the potential for restoring the natural biodiversity of the object. It is reported that the plantations and soil cover of the park of the 50th anniversary of VLKSM are very heterogeneous; they reflect the history of park establishment. The soil cover consists of three main types: sod, bog and urbanozem. It should be stressed that the best preserved part of the park is the plot with boggy soils around the pond. Herbaceous plants growing on the plot are very similar to the species composition of the ground cover of overgrown peat bogs. Over the 40 years since the foundation of the park, a high level of pollutants has been accumulated in the soil, which allows us to state that the negative impact occurs constantly. Consequently, the existing plantings are insufficient to protect visitors from vehicle emissions from the roads along Yasnaya and Shaumyana streets. There are enough natural elements in the park of the 50th anniversary of VLKSM; this should be taken into account in its redevelopment. There is an opportunity to restore the reservoir and return the plantings to their natural appearance. It is proposed to add elements of landscape decoration in the park.


Author(s):  
Nima Ahmadi ◽  
Farzan Sasangohar ◽  
Tariq Nisar ◽  
Valerie Danesh ◽  
Ethan Larsen ◽  
...  

Objective To identify physiological correlates to stress in intensive care unit nurses. Background Most research on stress correlates are done in laboratory environments; naturalistic investigation of stress remains a general gap. Method Electrodermal activity, heart rate, and skin temperatures were recorded continuously for 12-hr nursing shifts (23 participants) using a wrist-worn wearable technology (Empatica E4). Results Positive correlations included stress and heart rate (ρ = .35, p < .001), stress and skin temperature (ρ = .49, p < .05), and heart rate and skin temperatures (ρ = .54, p = .0008). Discussion The presence and direction of some correlations found in this study differ from those anticipated from prior literature, illustrating the importance of complementing laboratory research with naturalistic studies. Further work is warranted to recognize nursing activities associated with a high level of stress and the underlying reasons associated with changes in physiological responses. Application Heart rate and skin temperature may be used for real-time detection of stress, but more work is needed to validate such surrogate measures.


Author(s):  
E.N. Tyukhtina ◽  

The article deals with one of the most relevant topics of the modern city and ecology - the problem of landscaping in Volgograd. The city is characterized by an industrial urbanism, with a high concentration of population, saturation of production facilities and vehicles, which contributes to a high level of negative impact on the environment. The pace of greening of the city lags behind the pace of construction of the residential sector, and the existing green spaces do not meet the sanitary and hygienic, aesthetic requirements. The prospects of this problem are determined, the existing green spaces are considered, data on the climatic conditions of the city and the ecological situation are given. Improvement in the field of landscaping is associated with the scientifically sound placement of green spaces, taking into account all the factors that affect the growth and development of plants in the urban environment. Unfortunately, due to the uneven distribution of the assortment of trees and shrubs, the species composition of the central streets and squares of the city is extremely poor. The study focuses on the role of the botanical garden in urban landscaping and aims to create recommendations for the sorting of tree and shrub plants for urban landscaping. As a result of the analysis of the assortment of ornamental plants of the open ground of the botanical garden, the article proposed species and varieties of woody and shrubby plants that have passed the introduction tests for more than 5-10 years. Recommendations for sorting are aimed at improving the ecological situation of the city and greening the recreation areas of citizens. The proposed list of tree and shrub species cannot fully solve the problem of greening the city, but it will help to significantly enrich the composition of tree and shrub plants.


Author(s):  
M. Estrada ◽  
Manuel Camacho ◽  
César Benito

AbstractInter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.


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