scholarly journals Populasi Serangga Hama dan Artropoda Predator pada Padi Rawa Lebak Sumatera Selatan yang Diaplikasikan Bioinsektisida dari Beauveria bassiana dan Insektisida Sintetik

2019 ◽  
Vol 8 (1) ◽  
pp. 31-38
Author(s):  
Khoirul Ikhsanudin Hanif ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Tili Karenina ◽  
Erise Anggraini ◽  
...  

Hanif et al, 2019. Population of Pest Insects and Predatory Arthropods Inhabiting Freshwater Swamp Rice of South Sumatra Treated with Bioinsectide of Beauveria bassiana and a Synthetic Insecticide. JLSO (8)1:31-38.Entomopathogenic fungi, Beauveria bassiana, could  kill various species of insect pests, but  their impact on predatory arthropods needs to be studied. The aim of this study was to compare population of pest insects and predatory arthropods inhabiting rice sprayed with bioinsecticide of B. bassiana and conventional rice field using a synthetic insecticides. Bioinsecticide was made from conidia of B. bassiana and sprayed on rice canopy. The plot of conventional farmers was sprayed with synthetic insecticides made from Abamektrin. The results showed that the application of bioinsecticide of B. bassiana was not proven to reduce the pest insect population. The abundance of natural enemies of pest insects, such as  spiders and predatory insects was also more abundant in plots sprayed with  bioinsecticide than conventional plots that used synthetic insecticide. The synthetic insecticides have been shown to reduce the abundance of predatory insects and spiders. Bioinsecticides containing carrier of  liquid compost were proven to increase the growth and development of rice, which was an increase in the number of rice tillers per clump in plots applied by bioinsecticide. Thus, B. bassiana of bioinsecticide with liquid compost carrier material was safer and could maintain the abundance of predatory arthropods and increased rice growth.

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
Siti Herlinda ◽  
Ghanni Prabawati ◽  
Yulia Pujiastuti ◽  
Susilawati Susilawati ◽  
Tili Karenina ◽  
...  

Abstract. Herlinda S, Prabawati G, Pujiastuti Y, Susilawati, Karenia T, Hasbi, Irsan C. 2020. Herbivore insects and predatory arthropods in freshwater swamp rice field in South Sumatra, Indonesia sprayed with bioinsecticides of entomopathogenic fungi and abamectin. Biodiversitas 21: 3755-3768. Herbivore insect population and predatory arthropods in rice field may be effected by the application entomopathogenic fungi or synthetic insecticide. The objective of this research was to analyze individual quantity of herbivore insects and predatory arthropod inhabiting freshwater swamp rice fields treated with bioinsecticides and abamectin (commercial insecticide). This research was conducted in the freshwater swamp rice field located in Village Pelabuhan Dalam, Sub District Pemulutan, District Ogan Ilir, and South Sumatra. The experiment was arranged in a Completely Randomized Block Design consisted of four plots of treatment. The research used the bioinsecticides made from entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae, and Cordyceps military), and abamectin. Sampling to collect arthropods inhabiting rice canopy was also conducted using an entomological net in the study locations. This research found 12 families of herbivore insects with 22 species dominated by Nilaparvata lugens and Leptocorisa acuta and 32 species of spider belonged to eight families dominated by Tetragnatha virescens and Oxyopes matiensis. The species diversity of spider was higher in the plots of the bioinsecticide compared to that of the abamectin. Predatory insects found belonged to 14 species belonged to eight families dominated by species of Ophionea nigrofasciata, Verania discolor, and Paedorus fuscipe. The abundance of predatory insects in plots sprayed with the bioinsecticides was higher compared to that of the abamectin plot. The abundance and species diversity of predatory arthropod sprayed with bioinsecticide of B. bassiana, M. anisopliae, and C. militaris did not decrease, while the population of herbivore insect tended to decrease.


2020 ◽  
Vol 21 (5) ◽  
Author(s):  
KHOIRUL IKHSANUDIN HANIF ◽  
Siti Herlinda ◽  
CHANDRA IRSAN ◽  
YULIA PUJIASTUTI ◽  
GHANNI PRABAWATI ◽  
...  

Abstract. Hanif KI, Herlinda S, Irsan C, Pujiastuti Y, Prabawati G, Hasbi, Karenina T. 2020. The impact of bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy. Biodiversitas 21: 2124-2136. Bioinsecticides have been shown to reduce insect pest populations, but it is necessary to monitor the effect of bioinsecticide overdoses on non-target arthropods because they are abundant in rice fields. This study aimed to analyze the impact of bioinsecticide overdoses of B. bassiana on species diversity and abundance of non-target arthropods. Bioinsecticides derived from Beauveria bassiana were sprayed on the paddy every two weeks until the ripening stage, at a dose of 1, 2, 3, 4 L.ha-1, control (without insecticide), and abamectin as prescribed. The abundance of spiders and predatory arthropods did not decrease when bioinsecticides were applied but the abundance of spiders, especially Lycosidae and Salticidae dropped significantly when abamectin was applied. The abundance of the parasitoid decreased significantly when abamectin was applied, but the abundance of parasitoids applied by bioinsecticide was not significantly different from those of control. The abundance of insect pests started to decrease when bioinsecticide was applied to 2, 3 or 4 L.ha-1. The diversity species of non-target arthropods (spiders, predatory arthropods, and parasitoids) tended to decrease when it was applied bioinsecticides 3 and 4 L.ha-1 or abamectin, but their abundance remained high and no species dominance was found. For this reason, the correct dosage for suppressing insect pests abundance by not decreasing the abundance and diversity of non-target species is 2 L.ha-1 and applied when herbivore abundance is high.


2019 ◽  
Vol 20 (8) ◽  
Author(s):  
TILI KARENINA ◽  
Siti Herlinda ◽  
CHANDRA IRSAN ◽  
YULIA PUJIASTUTI

Abstract. Karenina T, Herlinda S, Irsan C, Pujiastuti Y. 2019. Abundance and species diversity of predatory arthropods inhabiting rice of refuge habitats and synthetic insecticide application in freshwater swamps in South Sumatra, Indonesia. Biodiversitas 20: 2375-2387. Rice cultivation in freshwater swamps in Indonesia is specific, among other things, there is no synthetic pesticide spraying and vegetable growing on rice field embankments. This specific cultivation technique can affect the abundance and diversity of rice-dwelling arthropods species. The study aimed to compare the abundance and species diversity of predatory arthropods inhabiting rice surrounded both by refugia and vegetables and by those being applied with synthetic insecticide in the rice field of freshwater swamps. This study used rice plots surrounded by refugia flower (Zinnia sp., Tagetes erecta, Cosmo caudatus, and Sesamum indicum); vegetables (Cucumis sativus, Vigna sinensis, Luffa acutangula, and Momordica charantia); untreated; and synthetic insecticide. Twenty-five herbivore species of 12 families, 34 spider species of 8 families, and 24 species of predatory insects of 12 families were found on rice during a planting season. The lowest herbivore population and spider abundance were found on synthetic insecticide sprayed rice and they were significantly different from those of refugia-rice plots. The most abundant spiders and predatory insects were found on rice surrounded by refugia and were not significantly different from those of rice surrounded by vegetables. The highest species diversity for spiders and predatory insects was found on rice surrounded by refugia, whereas the lowest species diversity was found on synthetic pesticide sprayed rice. Therefore, rice surrounded by refugia flowers and vegetables was the most appropriate for habitat and niche of predatory arthropods.


2019 ◽  
Vol 20 (10) ◽  
Author(s):  
GHANNI PRABAWATI ◽  
Siti Herlinda ◽  
YULIA PUJIASTUTI

Abstract. Prabawati G, Herlinda S, Pujiastuti Y. 2019. A comparative study on the arboreal arthropod abundance of rice fields between applied with fungal bioinsecticides and abamectin in the freshwater swamp of South Sumatra, Indonesia (Study case in the main and ratoon rice fields). Biodiversitas 20: 2921-2930. Ratoon rice has been cultivated by local farmers in South Sumatra and can be a source of habitat and niches for the main rice arthropods. The study aimed to compare the abundance of arthropods between main and rationed rice field applied with fungal bioinsecticides and abamectin. This study used bioinsecticides from fungi of Beauveria bassiana, Metarhizium anisopliae, and Cordyceps militaris and control using abamectin. Arboreal arthropods sampled using a sweep net. The abundance of arthropods in the main rice tends to be higher than in ratoon. Spiders found during the two rice seasons were Araneidae, Linyphiidae, Lycosidae, Oxyopidae, Salticidae, Theridiidae, Tetragnathidae, Theridiosomatidae, while predatory insects found were Mantidae, Coenagrionidae, Staphylinidae, Anthicidae, Latridiidae, Formicidae, Coccinellidae, Coccinellidae, Tettigoniidae, Miridae, Carabidae. The fungal bioinsecticides did not reduce the predatory arthropod abundance but the abamectin caused a decrease in the abundance of all guilds. The most drastic decrease was found in web-building spiders. The movement of hunting spiders and the predatory insects of the main to ratoon rice was faster than that of web-building spiders. The existence of ratoon rice could become habitats and niches for arthropods from the main rice. For this reason, controlling the herbivore insects could use bioinsecticides derived from entomopathogenic fungi.


2021 ◽  
Vol 807 (2) ◽  
pp. 022108
Author(s):  
Fitri ◽  
S N Aminah ◽  
T Abdullah ◽  
R Widarawati ◽  
N W Annisa

2013 ◽  
Vol 80 (3) ◽  
pp. 363-366 ◽  
Author(s):  
Luis Francisco Angeli Alves ◽  
Marina Andressa Formentini ◽  
André Luis Pereira Fanti ◽  
Maria Elena Schapovaloff ◽  
Ionete Lúcia Milani Barzotto

Gyropsylla spegazziniana is one of the most prominent pest insects of yerba mate culture in all production regions in Brazil, Argentina and Paraguay. Natural enemies have been recorded on G. spegazziniana nymphs and adults, including the Zoophthora radicans entomopathogenic fungus. Since there are no reports of Beauveria bassiana, the aim of this study was to register its pathogenicity with respect to this insect in a laboratory setting. Yerba mate branches were kept in glass flasks with water and we infested each leaf with 20 newly-emerged nymphs. We prepared three replicates per treatment. We sprayed conidia suspensions (1 × 109 conidia/mL) onto the branches, which we transferred to cages and kept in an acclimatized room (26 ± 1°C, 70 ± 10% of relative humidity (R.H.) with a photophase of 14 hours). We evaluated insect mortality daily, and after five days we observed a high level of pathogenicity due to the presence of the fungus, that could be visually observed on the cadaver. The total mortality varied from 25 to 70% (respectively for Unioeste 4 and CG 716) and the confirmed mortality was 30% for Unioeste 52, revealing the fungus' potential. However, more studies are necessary in order to evaluate the strains of this fungus, as well as other species.


Viruses ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 665 ◽  
Author(s):  
Charalampos Filippou ◽  
Inmaculada Garrido-Jurado ◽  
Nicolai Meyling ◽  
Enrique Quesada-Moraga ◽  
Robert Coutts ◽  
...  

The use of mycoviruses to manipulate the virulence of entomopathogenic fungi employed as biocontrol agents may lead to the development of novel methods to control attacks by insect pests. Such approaches are urgently required, as existing agrochemicals are being withdrawn from the market due to environmental and health concerns. The aim of this work is to investigate the presence and diversity of mycoviruses in large panels of entomopathogenic fungi, mostly from Spain and Denmark. In total, 151 isolates belonging to the genera Beauveria, Metarhizium, Lecanicillium, Purpureocillium, Isaria, and Paecilomyces were screened for the presence of dsRNA elements and 12 Spanish B. bassiana isolates were found to harbor mycoviruses. All identified mycoviruses belong to three previously characterised species, the officially recognised Beauveria bassiana victorivirus 1 (BbVV-1) and the proposed Beauveria bassiana partitivirus 2 (BbPV-2) and Beauveria bassiana polymycovirus 1 (BbPmV-1); individual B. bassiana isolates may harbor up to three of these mycoviruses. Notably, these mycovirus species are under distinct selection pressures, while recombination of viral genomes increases population diversity. Phylogenetic analysis of the RNA-dependent RNA polymerase gene sequences revealed that the current population structure in Spain is potentially a result of both vertical and horizontal mycovirus transmission. Finally, pathogenicity experiments using the Mediterranean fruit fly Ceratitis capitata showed no direct correlation between the presence of any particular mycovirus and the virulence of the B. bassiana isolates, but illustrated potentially interesting isolates that exhibit relatively high virulence, which will be used in more detailed virulence experimentation in the future.


2018 ◽  
Vol 33 (3-4) ◽  
pp. 221-231 ◽  
Author(s):  
Ivelina Nikolova ◽  
Natalia Georgieva

The effects of the synthetic insecticide Eforia 043 ZK (thiamethoxam+lambdacyhalothrin), applied alone and at 1/5 and 2/5 reduced doses in a mix with the mineral oil Akarzin, on Tychius flavus Beck. (Coleoptera: Curculionidae), Adelphocoris lineolatus Goeze (Hemiptera: Miridae), Acyrthosiphon pisum Harr. (Hemiptera: Aphididae), harmful thrips (Thysanoptera) and cicadas (Hemiptera: suborder Auchenorrhyncha) were studied. The efficacy of Eforia (applied alone and in reduced doses) was significantly higher against A. pisum, followed by A. lineolatus and harmful thrips, compared to cicadas. The insecticidal action of Akarzin had a better protective effect against A. pisum, followed by A. lineolatus, in comparison with thrips and cicadas. Combined application of the broad-spectrum insecticide Eforia provided good control against insect pests. The mix of Eforia?s reduced 1/5 dose with the mineral oil showed the highest toxicity throughout the reporting period. Eforia applied at the reduced dose of 2/5 with Akarzin followed in efficacy. A synergistic effect was observed in combinations and was defined as subadditive synergism. Reduced dosage of Eforia and decrease in negative environmental impact of the plant protection products is a friendly approach to integrated pest control. It is important to apply innovative formulations of plant protection products that are safer both for plants and the environment.


Author(s):  
Dayami Laguna Ávila ◽  
Carlos Pupo Feria ◽  
Gladia González Ramírez ◽  
Alina Espeek González

Insects pest of stored grains. Impact on food security of Las Tunas municipality, Cuba Resumen Con el objetivo de determinar las especies de insectos plaga en granos almacenados para la obtención de información que contribuyan al manejo de sus poblaciones y minimizar las pérdidas que influyen en la seguridad alimentaria de la población, se realizó un estudio en la Unidad 639 de la Empresa Mayorista de Productos Alimenticios del municipio Las Tunas, Cuba, en el período de enero del año 2011 hasta diciembre del año 2013. En el estudio se colectaron e identificaron 26 especies de insectos plaga en las naves, distribuidas en 19 géneros y 12 familias, pertenecientes a tres órdenes, de ellas, 23 especies cosmopolitas y de amplia distribución en Cuba. Las principales especies de insectos plaga encontradas fueron Tribolium castaneum; Ahasverus advena; Bruchus pisorum; Sitophilus oryzae; Ephestia sp. y Liposcelis sp. El hospedante con mayor frecuencia de aparición de las especies de insectos plaga fue el arroz, seguido por el frijol y el chícharo. Palabras clave: almacenes; cereal; plagas Abstract In order to determine the species of insect pests in stored grains to obtain information that contribute to the management of their populations and minimize losses that influence the food security of the population, a study was carried out in Unit 639 of the Company Wholesaler of Food Products of the municipality Las Tunas, Cuba, in the period from January 2011 until December 2013. The study collected and identified 26 species of insect pests in the ships, distributed in 19 genera and 12 families, belonging to three orders, of which 23 species are cosmopolitan and widely distributed in Cuba. The main species of pest insects found were Tribolium castaneum; Ahasverus advena; Bruchus pisorum; Sitophilus oryzae; Ephestia sp. and Liposcelis sp. The host with the highest frequency of appearance of insect pests was rice, followed by beans and peas. Keywords:  Insect pests; storage; food safety.


2020 ◽  
Vol 23 (2) ◽  
pp. 1-6
Author(s):  
Helben Ismat Mohammaed ◽  
Mohammed Saeed Mirza ◽  
Feyroz Ramadan Hassan

Beauveria bassiana is an important entomopathogenic fungus that used as a biocontrol agent of insect pests. Maintaining and preserving B. bassiana cultures is essential for the effective evaluation of its potential as microbial agent against insect pest, for biodiversity studies and also for exchange of fungal material between laboratories. In the present work we evaluated the suitability of four preservation materials based on gelatin and rice to maintain the viability of B. bassiana to be used as baits for insect’s control. The gelatin amended with sugar recorded the maximum viability after 70 days of storage as 98.3% compared to 63.3% on rice media. Effective control of 80% of the ants was observed after 10 days of feeding on gelatin amended with sugar and rice bait. The results demonstrated that treatment with formulations containing conidia of B. bassiana presents insecticidal activity against ant in addition of acting as preservation materials.


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