scholarly journals First report of Diplostomidae metacercariae (Trematoda: Digenea) in African catfish Clarias gariepinus (Siluriformes: Clariidae) in Brazil

2019 ◽  
Vol 28 (4) ◽  
pp. 677-684
Author(s):  
Juliana Souza Ribeiro ◽  
Francisco Carlos Rodrigues de Oliveira ◽  
Nicole Brand Ederli
Keyword(s):  
Clarias Gariepinus ◽  
Paratenic Host ◽  
Body Cavity ◽  
African Catfish ◽  
Analysis Software ◽  
First Report ◽  
Image Analysis Software ◽  
The Family ◽  
The City ◽  
Scanning Electron

Abstract Clarias gariepinus is a fish from North of South Africa and was later introduced in several countries, including Brazil. The present study aimed to describe the first report of Diplostomidae metacercariae in C. gariepinus in Brazil. For this, 30 C. gariepinus were captured in a lake in the city of Campos dos Goytacazes, RJ, Brazil. Fishes were euthanized using freezing and necropsied for collection of parasites. The organs were dissected and analyzed for the presence of parasites that were processed for light and scanning electron microscopy. Trematodes were stained with Semichon’s Carmine and Gomori’s Trichrome, observed and schematized under a light microscope with image analysis software. A total of 190 trematodes were collected from the gills, suprabranchial organs, heart, stomach, intestinal mesentery, liver and body cavity of the fish. The parasites had a foliaceous body divided by a discrete constriction, without genital primordia, and a holdfast organ present at the posterior region, typical of metacercariae of the family Diplostomidae. It was classified as the 'Diplostomulum' morphotype, based on the morphology of the reserve bladder structure. This is the first report of the metacercariae of Diplostomidae parasitizing C. gariepinus in the Americas. This fish acts as an intermediate or paratenic host of this digenean in Brazil.

scholarly journals Testicular Morphology and Sperm Motility in Cultured African Catfish (Clarias gariepinus) at Different Stages of Development

2016 ◽  
Vol 8 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Chidozie Nwabuisi OKOYE ◽  
Udensi Maduabuchi IGWEBUIKE ◽  
Anietie Francis UDOUMOH ◽  
Chinadindu Tochukwu OKEREKE
Keyword(s):  
Sperm Motility ◽  
Clarias Gariepinus ◽  
Body Cavity ◽  
The Body ◽  
Sperm Cells ◽  
African Catfish ◽  
Testicular Weight ◽  
Testicular Morphology ◽  
The Mean ◽  
Somatic Index

Testicular morphology and sperm motility were evaluated in cultured Clarias gariepinus (n = 25) purposively assigned to five groups according to their age. The results showed that the testes were paired, elongated, dorso-ventrally flattened structures, situated in the caudal aspects of the body cavity. The mean length of both right and left testes increased linearly with age, being significantly (p < 0.05) higher at 6 months than at 4 and 5 months of age, and also significantly (p < 0.05) higher at 8 months than at 6 months of age, while the mean weight and organo-somatic index of the catfish testes increased linearly until 6 months of age, after which no significant (p > 0.05) increase in the testicular weight and organo-somatic index was observed. Unidirectional progressive movement of spermatozoa was detected in the milt of C. gariepinus at 6, 7 and 8 months of age, but sperm cells were non-motile at 4 and 5 months of age. Histological sections showed seminiferous lobules, whose germinal epithelia were characterized by many cysts enclosing clones of sperm cells. Each cyst enclosed a clone of sperm cells at an identical stage of spermatogenesis. Spermatids and spermatozoa were present in the lumen of the seminiferous lobule. The obtained results indicate that the morphology of the testes of C. gariepinus is similar to the testes of members of the order Siluriformes, but sexual maturity and production of motile spermatozoa may be achieved at 6 months of age in the African catfish.

A technique to quantify and characterize the density of fibres and follicles in the skin of sheep

1995 ◽  
Vol 46 (8) ◽  
pp. 1525 ◽  
Author(s):  
BN Nagorcka ◽  
AE Dollin ◽  
DE Hollis ◽  
CD Beaton
Keyword(s):  
Low Cost ◽  
Skin Surface ◽  
Analysis Software ◽  
Sem Images ◽  
Fibre Bundles ◽  
Image Analysis Software ◽  
Skin Biopsies ◽  
The Cost ◽  
Non Destructive ◽  
Scanning Electron

A description is given of a non-destructive technique to count fibres and quantify their relative positions in the skin of sheep by making impressions of the skin surface of live sheep. Each impression contains within it clear morphological structures caused by individual wool fibres as they emerge from individual follicles, and clusters of wool fibres emerging from follicle bundles. Individual fibres and clusters of fibres can also be seen to be grouped together forming 'follicle groups'. The detailed accuracy of the impressions is confirmed by examining scanning electron microscope (SEM) images of a model of the skin obtained using the skin impression, and by examining SEM images of the impression itself. The impressions of individual fibres and clusters of fibres (produced from follicle bundles) appear to be countable using image analysis software. The new technique provides a low cost method (i.e. low relative to the cost of analysing skin biopsies) for measuring the density of fibres and fibre bundles, and the number of fibres per bundle in the skin. The measurements are expected to be of significant commercial interest to wool breeders.

On the Microstructure of Different Types of Particles in PM10 in Beijing-Tianjin-Tangshan City Group Agglomeration Atmosphere

2013 ◽  
Vol 448-453 ◽  
pp. 341-348 ◽  
Author(s):  
Shi Hong Cen ◽  
Xiao Yan Song ◽  
Long Yi Shao ◽  
Bing Yan ◽  
Yan Li Wu ◽  
...  
Keyword(s):  
Research Result ◽  
Urban Agglomeration ◽  
Spherical Particles ◽  
Distribution Characteristics ◽  
Analysis Software ◽  
Image Analysis Software ◽  
Mineral Particles ◽  
Different Types ◽  
City Group ◽  
Scanning Electron

This paper analyses the microstructure of different types of particles in PM10in Beijing-Tianjin-Tangshan urban agglomeration atmosphere in spring and summer by using field emission scanning electron microscopy (FESEM), in which the distribution characteristics of the size and number of the different types of particles in PM10are specifically analyzed by using image analysis software. The research result shows that the types of particles in Beijing-Tianjin-Tangshan urban agglomeration atmosphere are unknown particles, soot aggregates, mineral particles and spherical particles. According to the proportion of the three partials in the atmosphere, the three cities ranks as follows: in the aspect of unknown particles, Tianjin ranks first, which is followed by Tangshan and Beijing; in the aspect of soot aggregates, Tangshan ranks first ,which is followed by Beijing and Tianjin; in the aspect of mineral particles, Beijing ranks first ,which is followed by Tangshan and Tianjin; in the aspect of spherical particles, Beijing ranks first ,which is followed by Tangshan and Tianjin;

Ciliary count in chronic suppurative otitis media: comparative quantitative study between mucosal and squamous types using scanning electron microscopy and image analysis

2004 ◽  
Vol 118 (5) ◽  
pp. 343-347 ◽  
Author(s):  
Ahmed Atef ◽  
Essam Ezat Ayad
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Otitis Media ◽  
Quantitative Study ◽  
Chronic Suppurative Otitis Media ◽  
Analysis Software ◽  
Suppurative Otitis Media ◽  
Image Analysis Software ◽  
Scanning Electron

The objective of this study was to prove ciliary destruction in the middle-ear mucous membrane in cases of chronic suppurative otitis media (CSOM) and to compare both types of chronic suppurative otitis media with regard to the degree of ciliary destruction and ciliary count using objective quantitative techniques. The mucosa of the anterior mesotympanum over the promontory was sampled in 10 patients with mucosal CSOM and in another 10 patients with squamous type CSOM. Specimens were examined by scanning electron microscopy in combination with image analysis software techniques in order to study the cilia under higher magnifications and to calculate the ciliary area. Five patients with otosclerosis, no history suggestive of otitis media and normal ear drum appearance served as controls. Samples were taken and studied at the Faculty of Medicine of Cairo University. CSOM was found to be associated with significant ciliary destruction and this was more evident in the squamous type than in the mucosal type.

Effect of P and Ge doping on microstructure of Sn-0.3Ag-0.7Cu/Ni-P solder joints

2016 ◽  
Vol 28 (4) ◽  
pp. 215-221 ◽  
Author(s):  
Xingchen Yan ◽  
Kexin Xu ◽  
Junjie Wang ◽  
Xicheng Wei ◽  
Wurong Wang
Keyword(s):  
Design Methodology ◽  
Solder Bump ◽  
Solder Joints ◽  
Diffusion Rate ◽  
Analysis Software ◽  
Content Type ◽  
Image Analysis Software ◽  
Interfacial Intermetallic Compound ◽  
Ge Doping ◽  
Scanning Electron

Purpose The purpose of this paper is to comparatively investigate the microstructure and interfacial intermetallic compound (IMC) layer of Cu/SACPG/Ni and Cu/SAC0307/Ni solder joints after thermal aging. Design/methodology/approach The specimens were thermally aged at 150°C for 0, 24, 168 and 500 h. The microstructure and morphology of the interface IMC layer were observed by means of scanning electron microscope. The IMCs and the solder bump surface were analyzed by EDS. Moreover, the thickness of IMC layer was measured by using the image analysis software. Findings The morphology of IMC of Cu/SAC0307/Ni solder joint was consistent with that of the Cu/SACPG/Ni joint, which indicates that the addition of P and Ge had little effect on the IMC formation. The needle-like (Cu,Ni)6Sn5 was formed at the interface of solder/Ni solder joints. Meanwhile, the tiny particles inferred as Ag3Sn phase attached to the surface of (Cu,Ni)6Sn5. The growth rate of IMC layer of the Cu/SACPG/Ni joint was smaller than that of Cu/SAC0307/Ni joint with aging time increasing, which means the addition of trace P and Ge can slightly suppress the diffusion rate of the interfacial IMC. Originality/value There are no previous studies on the formation mechanism of the IMC layer of SAC0307 solder alloys with P and Ge addition.

scholarly journals First records of ectoparasitic insects (Diptera: Hippoboscoidea) of bats in the department of Caldas, Colombia

2020 ◽  
Vol 60 ◽  
pp. e20206018
Author(s):  
Jorge Raigosa Álvarez ◽  
Carolina García Osorio ◽  
Analía Gladys Autino ◽  
Lucimar Gomes Dias
Keyword(s):  
Central Andes ◽  
Mountain Range ◽  
First Report ◽  
Andes Mountain ◽  
The Family ◽  
The City ◽  
High Diversity

In Colombia, the taxonomical understanding of ectoparasites (Diptera: Streblidae and Nycteribiidae) of bats is scarce, despite the high diversity of hosts. This fact reflects the lack of important information on the group and poses the need for studies that expand the knowledge on bat ectoparasites in different regions of the country. The aim of this study was to contribute pioneer knowledge on bat ectoparasitic insects in the department of Caldas, located in the Central Andes Mountain range of Colombia. We assessed 318 bats from four eco parks in the city of Manizales, and collected 234 ectoparasitic insects. We provide the first report of the family Streblidae for Caldas, with seven genera and eight species: Anastrebla caudiferae Wenzel, 1976, Anatrichobius scorzai Wenzel, 1966, Exastinion oculatum Wenzel, 1976, Megistopoda proxima (Séguy‚ 1926), Paratrichobius longicrus Ribeiro, 1907, Paraeuctenodes similis Wenzel, 1976, Trichobius longipes Rudow, 1871, and Trichobius tiptoni Wenzel, 1976. In addition, we report the species Basilia ferrisi Schuurmans Stekhoven, 1931 and Basilia sp. for the family Nycteribiidae.

The effects of different image file formats and image-analysis software programs on dental radiometric digital evaluations

2001 ◽  
Vol 30 (1) ◽  
pp. 50-55 ◽  
Author(s):  
P Gürdal ◽  
C F Hildebolt ◽  
B G Akdeniz
Keyword(s):  
Image Analysis ◽  
Analysis Software ◽  
Image Analysis Software ◽  
Image File ◽  
File Formats ◽  
Software Programs

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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