Clopyralid Dose Response for Two Black Medic (Medicago lupulina) Growth Stages

2016 ◽  
Vol 30 (3) ◽  
pp. 717-724 ◽  
Author(s):  
Shaun M. Sharpe ◽  
Nathan S. Boyd ◽  
Peter J. Dittmar
Keyword(s):  
Dose Response ◽  
Growth Stage ◽  
Plant Size ◽  
Stem Length ◽  
Effective Dosage ◽  
Growth Stages ◽  
Response Curves ◽  
Measured Range ◽  
Large Growth ◽  
Small Growth

Black medic is a troublesome weed in commercial strawberry fields in Florida. It emerges during crop establishment from the planting holes punched in plastic mulches that are installed on raised beds. Clopyralid is registered for posttransplant applications at 140 to 280 g ae ha−1but growers typically report suppression, not control. An outdoor potted experiment was designed to model the black medic dose-response curve and determine the effect of plant size at application on control. Two plant sizes were selected: designated small (0.5- to 1-cm stem length) and large (3- to 6-cm stem length). Dose-response curves were generated using a log-logistic four-parameter model. At 22 d after treatment (DAT), there was a significant interaction between clopyralid rate and black medic growth stage on both epinasty (P = 0.0022) and chlorosis (P = 0.0055). The effective dosage to induce 90% (ED90) epinasty were 249.5 and 398.3 g ha−1for the small and large growth stages, respectively. The ED90 for chlorosis was 748.2 for the small growth stage, whereas the estimated value for the large was outside the measured range. For necrosis there was no significant effect of growth stage, and the ED90 was 1,856.3 g ha−1. The aboveground dry biomass ED90 for the small growth stage was 197.3 g ha−1, and the estimated ED90 value for the large was not within the measured range. Results indicate that clopyralid adequately controls black medic when applied at maximum label rates when stems were 0.5 to 1 cm long but not when plants were larger. Poor efficacy typically observed in commercial fields is likely due to black medic plant size or lack of herbicide coverage via shielding by strawberry plants.

Control of Volunteer Canola with Herbicides: Effects of Plant Growth Stage and Cold Acclimation

2006 ◽  
Vol 20 (2) ◽  
pp. 485-493 ◽  
Author(s):  
Anne Légère ◽  
Marie-Josée Simard ◽  
Eric Johnson ◽  
F. Craig Stevenson ◽  
Hugh Beckie ◽  
...  
Keyword(s):  
Cold Acclimation ◽  
Dose Response ◽  
Growth Stage ◽  
Field Experiments ◽  
Early Growth ◽  
Wheat Crop ◽  
Response Curves ◽  
Early Growth Stage ◽  
Herbicide Resistant ◽  
Phenoxy Herbicides

Phenoxy herbicides are frequently used to control volunteer canola populations. However, there have been claims that poor control could be due to cold acclimation of canola plants in the spring. The objective of this study was to determine whether cold acclimation or growth stage affected the response of canola volunteers to herbicides. In a growth room experiment, canola plants were prehardened and cold acclimated or were grown at 20/12 C and treated with one of six 2,4-D doses. Cold acclimation as achieved by this experiment affected upper and lower asymptotes of the dose–response curve but not the herbicide dose required to reduce canola weight by 50% relative to the nontreated control (GR50), indicating limited cold-related effects on canola tolerance to 2,4-D. Field experiments, conducted in the provinces of Québec and Saskatchewan, examined the effects of canola growth stage on the efficacy of 2,4-D, MCPA, and carfentrazone. Comparisons of the estimates from the dose–response curves confirmed that herbicide efficacy was consistently greater when canola plants were treated at an early growth stage, regardless of cultivar or herbicide used. The GR50estimates for canola plants treated at a later growth stage exceeded the recommended rates. Some canola plants grown as volunteers in a wheat crop survived 2,4-D or MCPA treatments at 0.5× and 1× rates and produced up to 148 seeds/m2. Efficient control of canola volunteers will be obtained when plants are sprayed at an early growth stage, but near-total control will be highly desirable in order to restrict seedbank buildup, particularly when dealing with canola cultivars with different herbicide-resistant traits.

Glyphosate-Resistant Horseweed (Conyza canadensis) in Mississippi

2004 ◽  
Vol 18 (3) ◽  
pp. 820-825 ◽  
Author(s):  
Clifford H. Koger ◽  
Daniel H. Poston ◽  
Robert M. Hayes ◽  
Robert F. Montgomery
Keyword(s):  
Growth Stage ◽  
Leaf Growth ◽  
Glyphosate Resistance ◽  
Plant Size ◽  
Growth Stages ◽  
Conyza Canadensis ◽  
Fold Level

Survival of horseweed in several glyphosate-tolerant cotton and soybean fields treated with glyphosate at recommended rates preplant and postemergence was observed in Mississippi and Tennessee in 2001 and 2002. Plants originating from seed collected from fields where horseweed escapes occurred in 2002 were grown in the greenhouse to the 5-leaf, 13- to 15-leaf, and 25- to 30-leaf growth stages and treated with the isopropylamine salt of glyphosate at 0, 0.025, 0.05, 0.1, 0.21, 0.42, 0.84, 1.68, 3.36, 6.72, and 13.44 kg ae/ha to determine if resistance to glyphosate existed in any biotype. All biotypes exhibited an 8- to 12-fold level of resistance to glyphosate when compared with a susceptible biotype. One resistant biotype from Mississippi was two- to fourfold more resistant than other resistant biotypes. Growth stage had little effect on level of glyphosate resistance. The glyphosate rate required to reduce biomass of glyphosate-resistant horseweed by 50% (GR50) increased from 0.14 to 2.2 kg/ha as plant size increased from the 5-leaf to 25- to 30-leaf growth stage. The GR50rate for the susceptible biotype increased from 0.02 to 0.2 kg/ha glyphosate. These results demonstrate that the difficult-to-control biotypes were resistant to glyphosate, that resistant biotypes could survive glyphosate rates of up to 6.72 kg/ha, and that plant size affected both resistant and susceptible biotypes in a similar manner.

scholarly journals Nitrogen Rate and Timing of Nitrogen Application in Poinsettia (Euphorbia pulcherrima Willd. Ex Klotz.)

HortScience ◽  
1994 ◽  
Vol 29 (11) ◽  
pp. 1309-1313 ◽  
Author(s):  
Mary Ann Rose ◽  
John W. White
Keyword(s):  
Leaf Area ◽  
Growth Stage ◽  
Floral Induction ◽  
Dry Weight ◽  
Plant Size ◽  
Growth Stages ◽  
N Availability ◽  
Regression Equations ◽  
Shoot Dry Weight ◽  
N Rates

`Celebrate 2' Poinsettias were grown for 8 weeks in a controlled-environment growth room until first signs of bract coloration. In growth stage I (GSI; weeks 1 through 4) low, medium, and high N rates (25, 75, and 125 mg N/liter, respectively) were applied by subirrigation (no leaching). Following floral induction [growth stage II (GSII), weeks 5 to 8], there were nine treatments: all possible combinations of the three N rates in GSI plus three rates (75, 125, and 175 mg N/liter) in GSII. Although >80% of shoot dry weight and >90% of total leaf area developed during growth GSII, reaching an acceptable plant size by week 8 depended on receiving adequate fertilization in growth GSI. In contrast, leaf chlorosis, noted in plants receiving the lowest rate in GSI, was rapidly reversed by increasing the N rate in GSII. Quadratic regression equations fitted to shoot dry weight and leaf area data predicted that using 125 mg N/liter in both growth stages gave maximum responses at week 8. However, using 75 mg N/liter in GSI and 125 mg N/liter in GSII also produced acceptable growth in poinsettias. Our results suggest that some growth restriction imposed by N availability during the first 4 weeks of growth may be acceptable and perhaps desirable to reduce growth regulator use and the environmental impact of overfertilization.

A Meta-Analysis on the Effects of 2,4-D and Dicamba Drift on Soybean and Cotton

Weed Science ◽  
2014 ◽  
Vol 62 (1) ◽  
pp. 193-206 ◽  
Author(s):  
J. Franklin Egan ◽  
Kathryn M. Barlow ◽  
David A. Mortensen
Keyword(s):  
Crop Yield ◽  
Dose Response ◽  
Yield Loss ◽  
Meta Analysis ◽  
Growth Stages ◽  
Response Curves ◽  
Synthetic Auxin ◽  
Final Yield ◽  
Dose Response Curves ◽  
Herbicide Drift

Commercial introduction of cultivars of soybean and cotton genetically modified with resistance to the synthetic auxin herbicides dicamba and 2,4-D will allow these compounds to be used with greater flexibility but may expose susceptible soybean and cotton cultivars to nontarget herbicide drift. From past experience, it is well known that soybean and cotton are both highly sensitive to low-dose exposures of dicamba and 2,4-D. In this study, a meta-analysis approach was used to synthesize data from over seven decades of simulated drift experiments in which investigators treated soybean and cotton with low doses of dicamba and 2,4-D and measured the resulting yields. These data were used to produce global dose–response curves for each crop and herbicide, with crop yield plotted against herbicide dose. The meta-analysis showed that soybean is more susceptible to dicamba in the flowering stage and relatively tolerant to 2,4-D at all growth stages. Conversely, cotton is tolerant to dicamba but extremely sensitive to 2,4-D, especially in the vegetative and preflowering squaring stages. Both crops are highly variable in their responses to synthetic auxin herbicide exposure, with soil moisture and air temperature at the time of exposure identified as key factors. Visual injury symptoms, especially during vegetative stages, are not predictive of final yield loss. Global dose–response curves generated by this meta-analysis can inform guidelines for herbicide applications and provide producers and agricultural professionals with a benchmark of the mean and range of crop yield loss that can be expected from drift or other nontarget exposures to 2,4-D or dicamba.

scholarly journals Early Postemergence Control of Woodland Bittercress (Cardamine flexuosa With.) and Yellow Woodsorrel (Oxalis stricta L.) with Dithiopyr and Isoxaben Combinations1

2018 ◽  
Vol 36 (3) ◽  
pp. 114-118
Author(s):  
Debalina Saha ◽  
S. Christopher Marble ◽  
Annette Chandler
Keyword(s):  
Weed Control ◽  
Growth Stage ◽  
Early Growth ◽  
Growth Stages ◽  
Fresh Weight ◽  
Weed Species ◽  
Leaf Stage ◽  
Large Growth ◽  
Cardamine Flexuosa ◽  
Weight Data

Abstract The objective of this research was to evaluate dithiopyr and isoxaben combinations and indaziflam (Marengo) for early postemergence control of woodland bittercress (Cardamine flexuosa) and yellow woodsorrel at 4 different early growth stages. Herbicides evaluated included sprayable formulations of isoxaben, dithiopyr + isoxaben, dithiopyr, indaziflam, and prodiamine + isoxaben without any surfactants. Woodland bittercress growth stages included seed production (extra-large), recently flowered (large), 6 to 9 leaf (medium) or in 2 to 5 leaf stage (small), while yellow woodsorrel growth stages included 8 to 12 leaf stage (extra-large), 4 to 6 leaf stage (large), 2 to 4 leaf stage (medium) and cotyledon to 1 leaf stage (small). Shoot fresh weight data showed all treatments provided ≤98% of woodland bittercress at the small stage. Dithiopyr + isoxaben (98%), isoxaben (90%), and indaziflam (93%) provided the highest level of woodland bittercress control at the medium stage and were the only treatments providing acceptable control (≥80%). In the large stage, dithiopyr + isoxaben provided acceptable control (80%) and outperformed other treatments. All treatments with the exception of isoxaben generally provided acceptable control of yellow woodsorrel up to the large growth stage. Only indaziflam (86% control) provided acceptable control at the extra-large stage. Index words: herbicide, postemergence weed control, container-grown plants, phytotoxic damages. Herbicides used in this study: isoxaben (Gallery® 4SC) N-[3-(1-ethyl-1-methylpropyl)- 5-isoxazolyl]-2,6-dimethoxybenzamide; dithiopyr (Dimension® 2EW) S,S'-dimethyl 2-(difluoromethyl)-4- (2-methylpropyl)-6-(trifluoromethyl)- 3,5-pyridinedicarbothioate; indaziflam (Specticle® FLO) N-[(1R,2S)-2,3-dihydro-2,6-dimethyl-1H-inden-1-yl]-6-(1-fluoroethyl)-1,3,5-triazine-2,4-diamine; prodiamine + isoxaben (Gemini® SC) 2,4-dinitro-N3,N3-dipropyl-6-(trifluoromethyl)-1,3-benzenediamine + N-[3-(1-ethyl-1-methylpropyl)- 5-isoxazolyl]-2,6-dimethoxybenzamide; dithiopyr + isoxaben (Dimension® + Gallery®) S,S'-dimethyl 2-(difluoromethyl)-4- (2-methylpropyl)-6-(trifluoromethyl)- 3,5-pyridinedicarbothioate + N-[3-(1-ethyl-1-methylpropyl)- 5-isoxazolyl]-2,6-dimethoxybenzamide. Weed species evaluated: woodland bittercress (flexuous bittercress) (Cardamine flexuosa With.); yellow woodsorrel (Oxalis stricta L.).

Dose-response Curves in the Fibrin Plate Assay Fibrinolytic Activity of Proteases

1974 ◽  
Vol 32 (02/03) ◽  
pp. 356-365 ◽  
Author(s):  
F Haverkate ◽  
D. W Traas
Keyword(s):  
Dose Response ◽  
Fibrinolytic Activity ◽  
Enzyme Concentration ◽  
Response Curves ◽  
Agar Gel ◽  
Porcine Tissue ◽  
Plate Assay ◽  
Fibrin Plate ◽  
Different Types ◽  
Dose Response Curves

SummaryIn the fibrin plate assay different types of relationships between the dose of applied proteolytic enzyme and the response have been previously reported. This study was undertaken to determine whether a generally valid relationship might exist.Trypsin, chymotrypsin, papain, the plasminogen activator urokinase and all of the microbial proteases investigated, including brinase gave a linear relationship between the logarithm of the enzyme concentration and the diameter of the circular lysed zone. A similar linearity of dose-response curves has frequently been found by investigators who used enzyme plate assays with substrates different from fibrin incorporated in an agar gel. Consequently, it seems that this linearity of dose-response curves is generally valid for the fibrin plate assay as well as for other enzyme plate bioassays.Both human plasmin and porcine tissue activator of plasminogen showed deviations from linearity of semi-logarithmic dose-response curves in the fibrin plate assay.

Factor VII Clotting Assay: Influence of Different Thromboplastins and Factor VII-Deficient Plasmas

1991 ◽  
Vol 65 (02) ◽  
pp. 160-164 ◽  
Author(s):  
Marina Poggio ◽  
Armando Tripodi ◽  
Guglielmo Mariani ◽  
Pier Mannuccio Mannucci ◽  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Dose Response ◽  
Response Curve ◽  
Ischemic Heart ◽  
Factor Vii ◽  
Response Curves ◽  
Assay Sensitivity ◽  
Clinical Laboratories ◽  
Coagulant Activity

SummaryBeing a putative predictor of ischemic heart disease, the measurement of factor VII (FVTI) coagulant activity will be presumably requested to clinical laboratories with increasing frequency. To assess the influence on FVII assays of different thromboplastins and FVII-deficient plasmas we compared performances of all possible combinations of 5 thromboplastins and 6 deficient plasmas. The reproducibility of the clotting times of the dose-response curves for human and rabbit thromboplastins were acceptable (CV lower than 7%), whereas bovine thromboplastin had a higher CV. Reproducibility was very similar for all deficient plasmas when they were used in combination with a given thromboplastin. Responsiveness of the dose-response curve did not depend on the deficient plasma but rather on the thromboplastin: one rabbit thromboplastin was the least responsive, the bovine thromboplastin the most responsive, the human and the remaining two rabbit thromboplastins had intermediate responsiveness. Assay sensitivity to cold-activated FVII varied according to the thromboplastin: the bovine thromboplastin was the most sensitive, the human thromboplastin the least sensitive, of the three rabbit thromboplastins two were relatively sensitive, one was almost insensitive. In conclusion, our results indicate that thromboplastin rather than deficient plasma is the crucial factor in the standardization of FVII assay.

OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1967 ◽  
Vol 56 (4) ◽  
pp. 619-625 ◽  
Author(s):  
Hans Jacob Koed ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Dose Response ◽  
Response Curve ◽  
Dose Response Curve ◽  
Human Origin ◽  
Response Curves ◽  
Significant Difference ◽  
The Mean ◽  
Different Levels

ABSTRACT Comparison of the dose-response curves for LH of ovine origin (NIH-LH-S8) and of human origin (IRP-HMG-2) using the OAAD test showed a small, though statistically significant difference, the dose-response curve for LH of human origin being a little flatter. Two standard curves for ovine LH obtained with 14 months' interval, were parallel but at different levels of ovarian ascorbic acid. When the mean ascorbic acid depletions were calculated as percentages of the control levels, the two curves for NIH-LH-S8 were identical. The use of standards of human origin in the OAAD test for LH activity of human preparations is recommended.

BIOASSAY OF THYROID HORMONE USING HYPOTHYROID TADPOLES

1962 ◽  
Vol 41 (1) ◽  
pp. 143-153 ◽  
Author(s):  
U. Henriques
Keyword(s):  
Thyroid Hormone ◽  
Dose Response ◽  
Developmental Rate ◽  
Response Curves ◽  
Sodium Salts ◽  
Dose Response Curves

ABSTRACT A bioassay of thyroid hormone has been developed using Xenopus larvae made hypothyroid by the administration of thiourea. Only tadpoles of uniform developmental rate were used. Thiourea was given just before the metamorphotic climax in concentrations that produced neoteni in an early metamorphotic stage. During maintained thiourea neotoni, 1-thyroxine and 1-triiodothyronine were added as sodium salts to the water for three days and at the end of one week the stage of metamorphosis produced was determined. In this way identical dose-response curves were obtained for the two compounds. No qualitative differences between their effects were noted except that triiodothyronine seemed more toxic than thyroxine in equivalent doses. Triiodothyronine was found to be 7–12 times as active as thyroxine.

ÜBER DIE ANTAGONISTISCHE WIRKUNG VON TRIJODTHYRONIN UND PROGESTERON AUF DEN PREDNISOLONEFFEKT AM EPIPHYSENKNORPEL (PRÜFUNG DER REISSFESTIGKEIT)

1963 ◽  
Vol 42 (1) ◽  
pp. 29-38 ◽  
Author(s):  
L. Ther ◽  
H. Schramm ◽  
G. Vogel
Keyword(s):  
Tensile Strength ◽  
Dose Response ◽  
Single Injection ◽  
Relative Effectiveness ◽  
Prednisolone Acetate ◽  
Response Curves ◽  
Deoxycorticosterone Acetate ◽  
Weight Range ◽  
Diuretic Action ◽  
Maximal Load

ABSTRACT Longitudinal tension exerted on a killed rat's femur results always in rupture on the same place viz: the distal epiphyseal line. The power required to produce this effect can be measured with a simple dynamometer. The tensile strength increases with advancing age and during exsiccation (thirst). In untreated rats (weight range 100–120 g) this value amounts to about 1.5–1.8 kg. Tensile strength of the femoral epiphyseal line may be influenced by hormones. Corticosteroids such as prednisolone acetate and cortisol acetate increase the level of maximal load to more than 2.0 kg. In general the effect of a single injection reaches a climax after 48 hours. Dose-response-curves can be established and relative effectiveness may be gathered therefrom. Deoxycorticosterone acetate likewise increases tensile strength in spite of the fact that contrary to prednisolone exhibiting diuretic action this compound has a water retaining effect. Triiodothyronine and progesterone when given alone produce a slight decrease in tensile strength. The effect of prednisolone when given simultaneously with one of these two compounds is antagonized: this phenomenon is more pronounced in the case of triiodothyronine.

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