Role of anb3 Integrin Receptor in the Invasive Potential of Human Cervical Cancer (SiHa) Cells

Basic helix-loop-helix (BHLH) transcription factors differentiated embryonic chondrocyte gene 1 (DEC1) and gene 2 (DEC2) regulate circadian rhythms, apoptosis, epithelial mesenchymal transition (EMT), invasions and metastases in various kinds of cancer. The stem cell markers SOX2 and c-MYC are involved in the regulation of apoptosis and poor prognosis. In cervical cancer, however, their roles are not well elucidated yet. To determine the function of these genes in human cervical cancer, we examined the expression of DEC1, DEC2, SOX2 and c-MYC in human cervical cancer tissues. In immunohistochemistry, they were strongly expressed in cancer cells compared with in non-cancerous cells. Notably, the strong rate of DEC1 and SOX2 expressions were over 80% among 20 cases. We further examined the roles of DEC1 and DEC2 in apoptosis. Human cervical cancer HeLa and SiHa cells were treated with cisplatin—HeLa cells were sensitive to apoptosis, but SiHa cells were resistant. DEC1 expression decreased in the cisplatin-treated HeLa cells, but had little effect on SiHa cells. Combination treatment of DEC1 overexpression and cisplatin inhibited apoptosis and affected SOX2 and c-MYC expressions in HeLa cells. Meanwhile, DEC2 overexpression had little effect on apoptosis and on SOX2 and c-MYC expressions. We conclude that DEC1 has anti-apoptotic effects and regulates SOX2 and c-MYC expressions on apoptosis.

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Effect of All-trans-Retinoic Acid on Integrin Receptors of Human Cervical Cancer (SiHa) Cells

Gynecologic Oncology ◽

10.1006/gyno.1999.5574 ◽

1999 ◽

Vol 75 (2) ◽

pp. 215-221 ◽

Cited By ~ 7

Author(s):

Nibedita Chattopadhyay ◽

Subrata Ray ◽

Nupur Biswas ◽

Amitava Chatterjee

Keyword(s):

Cervical Cancer ◽

Retinoic Acid ◽

Integrin Receptors ◽

Siha Cells ◽

Trans Retinoic Acid ◽

All Trans Retinoic Acid ◽

Human Cervical Cancer

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Inhibitory effect of genistein on the invasive potential of human cervical cancer cells via modulation of matrix metalloproteinase-9 and tissue inhibitiors of matrix metalloproteinase-1 expression

Cancer Epidemiology ◽

10.1016/j.canep.2012.07.005 ◽

2012 ◽

Vol 36 (6) ◽

pp. e387-e393 ◽

Cited By ~ 31

Author(s):

Arif Hussain ◽

Geetganga Harish ◽

Sathyen Alwin Prabhu ◽

Javeria Mohsin ◽

Munawwar Ali Khan ◽

...

Keyword(s):

Cervical Cancer ◽

Matrix Metalloproteinase ◽

Cancer Cells ◽

Inhibitory Effect ◽

Invasive Potential ◽

Matrix Metalloproteinase 1 ◽

Cervical Cancer Cells ◽

Human Cervical Cancer Cells ◽

Metalloproteinase 9 ◽

Human Cervical Cancer

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MicroRNA-222 promotes the proliferation and migration of cervical cancer cells

Clinical & Investigative Medicine ◽

10.25011/cim.v37i3.21380 ◽

2014 ◽

Vol 37 (3) ◽

pp. 131 ◽

Cited By ~ 15

Author(s):

Yi Sun ◽

Bo Zhang ◽

Jiajing Cheng ◽

Yi Wu ◽

Fing Xing ◽

...

Keyword(s):

Cervical Cancer ◽

Phosphatase And Tensin Homolog ◽

Migration Assay ◽

Tensin Homolog ◽

Siha Cells ◽

Non Coding Rna ◽

Cervical Cancer Cells ◽

And Migration ◽

Proliferation And Migration

Purpose: This study aimed to investigate the role of small non-coding RNA-222 (microRNA-222; miR-222) in the development of cervical cancer (CC). Methods: Normal and CC specimens were obtained from 18 patients. HeLa and SiHa cells were grown in Dulbecco’s modified Eagle’s medium. RT–PCR, Western blot, migration assay, flow cytometry and immunofluorescence microscopy were used for analyses. Results: When compared with normal cervical tissues, miR-222 was upregulated in human CC, and the extent of up-regulation was associated with the extent and depth of CC invasion. Expression of miR-222 was inversely related to the expression of phosphatase and tensin homolog (PTEN) and p27. The reduced the expression of PTEN and p27 by miR-222 in HeLa cells and SiHa cells was associated with increased proliferation and migration of CC cells. The expression of proteins (E-cadherin and paxillin) related to the proliferation and migration was also elevated. Conclusion: MiR-222 plays an important role in the tumorigenesis of CC, possibly by specifically down-regulating p27Kip1 and PTEN. Our findings suggest that miR-222 may serve as a new therapeutic target in CC.

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Baicalein inhibits the invasion of human cervical cancer cells by inhibiting the hedgehog/Gli signaling pathway

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i1.18 ◽

2020 ◽

Vol 19 (1) ◽

pp. 115-120

Author(s):

Hai Yang ◽

Jiyi Xia ◽

Yan Li ◽

Yong Cao ◽

Li Tang ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Signaling Pathway ◽

Hela Cells ◽

Colony Formation ◽

Diphenyltetrazolium Bromide ◽

Cervical Cancer Cells ◽

Human Cervical Cancer Cells ◽

Human Cervical Cancer

Purpose: To identify the role of baicalein in human cervical cancer and to determine whether baicalein treatment affects hedgehog/Gli signaling pathway. Methods: Cell proliferation was evaluated by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and colony formation assays. Cell death rate was assessed by PI-staining and FACS assay. Furthermore, cell invasion was assessed by Transwell assay while the levels of the key proteins were measured by western blotting analysis. Results: Baicalein suppressed the viability and proliferation of HeLa cells. The colony formation ability and relative migration rate were significantly decreased in the HeLa cells treated with 50 μM baicalein. Furthermore, the levels of Shh, Gli1, MMP-9, and VEGF declined significantly in baicalein-treated cells. Conclusion: The results demonstrate that baicalein inhibits the growth and invasiveness of cervical cancer cells partly by suppressing the activation of hedgehog/Gli signaling pathway in a concentrationdependent manner. Keywords: Cervical cancer, baicalein, hedgehog/Gli pathway, MMP-9

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SIRT1 promotes proliferation, migration, and invasion of cervical cancer cell and is upregulated by human papillomavirus 16 E7 oncoprotein

Gynecologic and Obstetric Investigation ◽

10.1159/000520642 ◽

2021 ◽

Author(s):

Yujing Wang ◽

Jing Wang ◽

Chunmei Liu ◽

Min Li

Keyword(s):

Cervical Cancer ◽

Stress Responses ◽

Migration And Invasion ◽

Cancer Tissue ◽

Sirt1 Expression ◽

Human Papillomavirus 16 ◽

Siha Cells ◽

Silent Information Regulator 1 ◽

Cancer Tissues

SIRT1 (silent information regulator 1), a NAD+-dependent III class histone deacetylase, plays crucial roles in cell proliferation, apoptosis, senescence, metabolism, and stress responses. Nevertheless, the role of SIRT1 in tumorigenesis remains unclear. In the present study, we measured expression levels of SIRT1 and HPV16 E7 protein in cervical cancer tissue and calculated their correlations. We measured the effect of silencing SIRT1 on the proliferation, migration, invasion, and apoptosis in human cervical cancer SiHa cells. Immunohistochemistry results revealed that the expression of SIRT1 was upregulated with progression from CINII-III to cervical cancer, but was not expressed in normal cervical tissues and CINI. There was a positive correlation between SIRT1 expression and HPV16 E7 expression in cervical cancer tissues, and silencing of HPV16 E7 downregulated the expression of SIRT1. Depletion of SIRT1 significantly downregulated SIRT1 expression, and inhibited proliferation, migration, and invasion of SiHa cells, inducing apoptosis. Taken together, the data suggest that SIRT1 promotes cervical cancer carcinogenesis. SIRT1 inhibition is a potential treatment strategy for cervical cancer.

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