Optical Microscopy and Scanning Electron Microscopy on the Surface of Rice Callus after Treatment with Cell Wall Degrading Enzymes

2001 ◽  
Vol 4 (2) ◽  
pp. 145-150 ◽  
Author(s):  
Tadahiko Sato ◽  
Oh Chang Kwon ◽  
Hiroshi Miyake ◽  
Takeshi Taniguchi ◽  
Eizo Maeda
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Optical Microscopy ◽  
Cell Wall Degrading Enzymes ◽  
Rice Callus ◽  
Degrading Enzymes ◽  
Scanning Electron

Determination of cotton fiber maturity and linear density (fineness) by examination of fiber cross-sections. Part 2: A comparison optical and scanning electron microscopy

2014 ◽  
Vol 84 (18) ◽  
pp. 1939-1947 ◽  
Author(s):  
Geoffrey RS Naylor ◽  
Margaret Pate ◽  
Graham J Higgerson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Optical Microscopy ◽  
Cross Sections ◽  
Linear Density ◽  
Wall Area ◽  
Density Values ◽  
Fiber Maturity ◽  
Scanning Electron

Previous researchers established a set of reference cottons with known fiber maturity and linear density (fineness) values based on the analysis of a large number of individual transverse fiber cross-sections viewed under the optical microscope. Part 1 identified that the limited optical resolution of the captured images may be the source of a significant systematic error in the assigned values of cell wall area and hence fiber maturity and linear density values. In this paper the optical microscopy technique was implemented. Individual cross-sections were measured using this approach and also higher resolution and higher magnification images were obtained using scanning electron microscopy. It was found that the data obtained from optical microscopy were similar to the SEM data, with the perimeter being 2% smaller, the cell wall area being 6% larger and the maturity ratio values being 8% higher. It was concluded that the combined approach of utilizing SEM in conjunction with optical imaging is a useful approach for verifying and perhaps correcting the data obtained from optical imaging. Further the SEM images highlighted that the current experimental protocol does not adequately address the challenge of ensuring that the fibers are mounted normal to the plane of cutting the transverse cross-section. Modeling demonstrated that while maturity ratio values are relatively insensitive to this misalignment, measured cell wall area values and hence fiber linear density values will be overestimated. This may be the major source of error associated with the technique and warrants further attention in future studies.

Digital imaging: When should one take the plunge?

1996 ◽  
Vol 54 ◽  
pp. 602-603
Author(s):  
John F. Mansfield
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Optical Microscopy ◽  
Digital Imaging ◽  
Storage Devices ◽  
Major Benefit ◽  
Transmission Electron ◽  
New Instrument ◽  
Scanning Electron

The current imaging trend in optical microscopy, scanning electron microscopy (SEM) or transmission electron microscopy (TEM) is to record all data digitally. Most manufacturers currently market digital acquisition systems with their microscope packages. The advantages of digital acquisition include: almost instant viewing of the data as a high-quaity positive image (a major benefit when compared to TEM images recorded onto film, where one must wait until after the microscope session to develop the images); the ability to readily quantify features in the images and measure intensities; and extremely compact storage (removable 5.25” storage devices which now can hold up to several gigabytes of data).The problem for many researchers, however, is that they have perfectly serviceable microscopes that they routinely use that have no digital imaging capabilities with little hope of purchasing a new instrument.

Some small-sized Cosmarium (Conjugatophyceae, Desmidiales) from sphagnum bogs of Moscow Region

2013 ◽  
Vol 47 ◽  
pp. 13-20
Author(s):  
O. V. Anissimova
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Moscow Region ◽  
Biological Station ◽  
Sphagnum Bogs ◽  
Different Seasons ◽  
Scanning Electron

Algae samples were collected during different seasons from 1997 to 2011 in two swamps located at Zvenigorod Biological Station in Moscow Region. There were found 25 Cosmarium species and varieties, 9 taxa of them being new to the region. Descriptions of the taxa were specified by observation of cell wall ornamentation with light and scanning electron microscopy. Original descriptions, photos and drawings of algae are presented.

Investigation of Die Tilting of Packages with Single and Multi-chips

2018 ◽  
Author(s):  
Lo Chea Wee ◽  
Tan Sze Yee ◽  
Gan Sue Yin ◽  
Goh Cin Sheng
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Beam ◽  
Optical Microscopy ◽  
Device Performance ◽  
Electronic Components ◽  
Area Of Interest ◽  
On Chip ◽  
Scanning Electron

Abstract Advanced package technology often includes multi-chips in one package to accommodate the technology demand on size & functionality. Die tilting leads to poor device performance for all kinds of multi-chip packages such as chip by chip (CbC), chip on chip (CoC), and the package with both CbC and CoC. Traditional die tilting measured by optical microscopy and scanning electron microscopy has capability issue due to wave or electron beam blocking at area of interest by electronic components nearby. In this paper, the feasibility of using profilemeter to investigate die tilting in single and multi-chips is demonstrated. Our results validate that the profilemeter is the most profound metrology for die tilting analysis especially on multi-chip packages, and can achieve an accuracy of <2μm comparable to SEM.

The External Morphology of the Eggs of Culex (Culex) saltanensis (Diptera: Culicidae) Under Scanning Electron Microscopy

2020 ◽  
Author(s):  
J R Santos-Mallet ◽  
T D Balthazar ◽  
A A Oliveira ◽  
W A Marques ◽  
A Q Bastos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Optical Microscopy ◽  
Osmium Tetroxide ◽  
Neotropical Region ◽  
External Morphology ◽  
Metal Supports ◽  
Scanning Electron

Abstract The aim of the present study was to describe the morphology of the eggs of Culex (Culex) saltanensis Dyar that occurs in the Neotropical region. Eggs of the Cx. (Cux.) saltanensis were collected at the Mata Atlântica FIOCRUZ campus, fixed in 1% osmium tetroxide, prepared for mounting on metal supports, observed under a scanning electron microscope, and described morphologically. The eggs had a coniform shape with a length of approximately 0.5 mm (505–510 µm) and a width in the median portion of 117 µm (113–123 µm). Upper portion is lined with tubers of irregular shape and varying sizes (0.64–1.31 µm), located on a cross-linked matrix forming bands observed under optical microscopy. The micropyle is encased in a necklace of approximately 6.6-µm plates arranged in a flower-like shape. Comparing Cx. (Cux.) saltanensis eggs with several species of different genera, important divergent characteristics can be observed. However, this study points to the need for new descriptions of eggs of species belonging to the same subgenus in order to analyze if there will be differences between them. Culex (Cux.) saltanensis eggs have particular characteristics not observed in eggs of other Culicidae genera.

Damage Analysis of Heat Resistant Steels

2007 ◽  
Vol 537-538 ◽  
pp. 303-306
Author(s):  
Tamás Bíró ◽  
László Dévényi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Optical Microscopy ◽  
Damage Analysis ◽  
Resistant Steel ◽  
Heat Resistant Steel ◽  
Advantages And Disadvantages ◽  
Heat Resistant ◽  
Heat Resistant Steels ◽  
Scanning Electron

This paper shows the result of some metallographical examinations that have been carried out on low-alloyed Cr-Mo-V heat resistant steel. The aim of this research is to present and compare the advantages and disadvantages of the mainly applied metallographical methods. These techniques are optical microscopy, scanning electron microscopy, replica method and special applications of these methods. We have proved that using the investigated methods together gives much more information about the lifetime of the specimen than using these techniques particularly.

scholarly journals Digital Imaging: When Should One Take The Plunge?

1997 ◽  
Vol 5 (4) ◽  
pp. 14-15
Author(s):  
John F. Mansfield
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Optical Microscopy ◽  
Digital Imaging ◽  
High Quality ◽  
Storage Devices ◽  
Major Benefit ◽  
Transmission Electron ◽  
Scanning Electron

The current imaging trend in optical microscopy, scanning electron microscopy (SEM) or transmission electron microscopy (TEM) is to record all data digitally. Most manufacturers currently market digital acquisition systems with their microscope packages. The advantages of digital acquisition include: almost instant viewing of the data as a high-quality positive image (a major benefit when compared to TEM images recorded onto film, where one must wait until after the microscope session to develop the images); the ability to readily quantify features in the images and measure intensities; and extremely compact storage (removable 5.25” storage devices which now can hold up to several gigabytes of data).

Identification of Nd163 phase in melt-textured NdBa2Cu3O7−δ bulk samples

2003 ◽  
Vol 18 (9) ◽  
pp. 2050-2054 ◽  
Author(s):  
Marcello Gombos ◽  
Vicente Gomis ◽  
Anna Esther Carrillo ◽  
Antonio Vecchione ◽  
Sandro Pace ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Optical Microscopy ◽  
Room Temperature ◽  
Polarized Light ◽  
Energy Dispersive ◽  
X Ray ◽  
Spontaneous Formation ◽  
The Stability ◽  
Scanning Electron

In this work, we report on the observation of Nd1Ba6Cu3O10,5 (Nd163) phase of the NdBaCuO system in melt-textured Nd123 bulk samples grown from a mixture of Nd123 and Nd210 phase powders. The observation was performed with polarized light optical microscopy and scanning electron microscopy–energy dispersive x-ray analyses. Images of the identified phase crystals show an aspect quite different from Nd422 crystals. Unexpectedly, Nd163 was individuated, even in “pure” Nd123 samples. Moreover, after long exposure to air, Nd163 disappeared completely in samples synthesized from powders containing Nd210. Thermogravimetry analyses of powders show that the stability of this phase in air is limited to temperatures higher than 900 °C, so Nd163 is unstable and highly reactive at room temperature. Moreover, an explanation of the observation of Nd163 in Nd210 free samples, based on the spontaneous formation of Nd163 phase in a Nd123 melt, is proposed.

scholarly journals Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes

2021 ◽  
Vol 12 ◽  
Author(s):  
Sergey Mursalimov ◽  
Nobuhiko Ohno ◽  
Mami Matsumoto ◽  
Sergey Bayborodin ◽  
Elena Deineko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Three Dimensional ◽  
Nuclear Migration ◽  
Male Meiosis ◽  
Face Scanning ◽  
Block Face ◽  
Plant Meiosis ◽  
Scanning Electron

Serial block-face scanning electron microscopy (SBF-SEM) was used here to study tobacco male meiosis. Three-dimensional ultrastructural analyses revealed that intercellular nuclear migration (INM) occurs in 90–100% of tobacco meiocytes. At the very beginning of meiosis, every meiocyte connected with neighboring cells by more than 100 channels was capable of INM. At leptotene and zygotene, the nucleus in most tobacco meiocytes approached the cell wall and formed nuclear protuberances (NPs) that crossed the cell wall through the channels and extended into the cytoplasm of a neighboring cell. The separation of NPs from the migrating nuclei and micronuclei formation were not observed. In some cases, the NPs and nuclei of neighboring cells appeared apposed to each other, and the gap between their nuclear membranes became invisible. At pachytene, NPs retracted into their own cells. After that, the INM stopped. We consider INM a normal part of tobacco meiosis, but the reason for such behavior of nuclei is unclear. The results obtained by SBF-SEM suggest that there are still many unexplored features of plant meiosis hidden by limitations of common types of microscopy and that SBF-SEM can turn over a new leaf in plant meiosis research.

Effect of KBF4 Addition on the Microstructure of Mg-6Zn-1Si Alloy

2012 ◽  
Vol 535-537 ◽  
pp. 996-999 ◽  
Author(s):  
Bao Feng Zhang ◽  
Qi Chen ◽  
Xiao Wei Chen ◽  
Hong Liang Zhao
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Optical Microscopy ◽  
Energy Dispersive Spectrometer ◽  
Energy Dispersive ◽  
Chinese Script ◽  
Mg2si Phase ◽  
Small Block ◽  
Average Size ◽  
Scanning Electron

In this study, the effect of KBF4 addition on the microstructure of the Mg-6Zn-1Si alloy has been investigated by Optical Microscopy (OM), Scanning Electron Microscopy (SEM) with Energy Dispersive Spectrometer(EDS). At 1.5wt% KBF4 addition, the morphology of Mg2Si phase completely changed from Chinese script type into uniformly, dispersedly distributed graininess or small block, with the average size of the Mg-6Zn-1Si alloy decreasing from 135μm to 82μm.

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