Determination of the fluorescence intensity of coenzymes NADH and FAD in the skeletal muscle of the rat depending on the post-mortem interval

2020 ◽  
Vol 63 (1) ◽  
pp. 31
Author(s):  
A. S. Babkina ◽  
D. V. Sundukov ◽  
A. M. Golubev ◽  
I. A. Ryzhkov ◽  
Z. I. Tsokolaeva ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Fluorescence Intensity ◽  
Post Mortem ◽  
Post Mortem Interval

Post-mortem interval estimative through determination of catalase and Δ-aminolevulinate dehydratase activities in hepatic, renal, skeletal muscle and cerebral tissues of Swiss mice

Biomarkers ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 478-483
Author(s):  
Jaini J. Paltian ◽  
Caren A. R. da Fonseca ◽  
Mikaela P. Pinz ◽  
Cristiane Luchese ◽  
Ethel Antunes Wilhelm
Keyword(s):  
Skeletal Muscle ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Swiss Mice ◽  
Aminolevulinate Dehydratase

scholarly journals PATTERNS OF CHANGES IN FLUORESCENCE OF NADH AND FAD COENZYMES AND THEIR RELATIONSHIP IN SKELETAL MUSCLE IN EARLY POST-MORTEM PERIOD (EXPERIMENTAL STUDY)

2020 ◽  
Author(s):  
Anastasia S. Babkina
Keyword(s):  
Skeletal Muscle ◽  
Mathematical Models ◽  
Fluorescence Intensity ◽  
Statistical Significance ◽  
Linear Regression Analysis ◽  
Post Mortem ◽  
Redox Ratio ◽  
Measuring Device ◽  
Nadh Fluorescence ◽  
The Relationship

Abstract. The paper presents changes and mathematical models of autofluorescence of reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) and their redox ratio (RR) in the rat skeletal muscle during the first 24 hours after death. Aim. To establish a pattern of change in the fluorescence intensity of NADH and FAD coenzymes, as well as their relationship in the skeletal muscle during the first 24 hours after death using mathematical modeling. Material and methods. The experiment was performed on Sprague Dawley rats. The fluorescence intensity of FAD and NADH coenzymes in skeletal muscle was measured in situ in living rats under general anesthesia, 5 minutes after euthanasia, and at intervals of 1.5-3 h during the 24-hour postmortem period. To estimate the fluorescence intensity of NADH and FAD coenzymes and to calculate the RR, the Lasma MC-3 fluorescence measuring device with special software was used. The obtained data were analyzed by non-linear regression analysis. The summary, accuracy estimation and significance of the regression equation coefficients were assessed using SigmaPlot 10.0 software. The significance of the regression model was tested using the Fisher F-criterion. Results. During the first 3 hours of the postmortem period, an increase in the mean values of RR and NADH fluorescence was detected, starting from 4.5 and till 24 hours post-mortem their gradual decrease was observed. The relationship between NADH, RR and time after death is characterized by the Weibull equation. The statistical significance of NADH and RR models, based on the obtained equations was considered high. Conclusion. The analysis of the obtained data allowed to create mathematical models describing the relationship between RR and NADH fluorescence intensity and time after death, which confirms non-randomness and regularity of the discovered patterns.time of death; NADH; FAD; auto-fluorescence; redox ratio; early post-mortem changes

scholarly journals The Role of DNA Degradation in the Estimation of Post-Mortem Interval: A Systematic Review of the Current Literature

2020 ◽  
Vol 21 (10) ◽  
pp. 3540 ◽  
Author(s):  
Pamela Tozzo ◽  
Salvatore Scrivano ◽  
Matteo Sanavio ◽  
Luciana Caenazzo
Keyword(s):  
Analytical Techniques ◽  
Dna Degradation ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Target Organs ◽  
The One ◽  
Definition Of ◽  
Meta Analyses

The determination of the post-mortal interval (PMI) is an extremely discussed topic in the literature and of deep forensic interest, for which various types of methods have been proposed. The aim of the manuscript is to provide a review of the studies on the post-mortem DNA degradation used for estimating PMI. This review has been performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses and the PRISMA Guidelines. Several analytical techniques have been proposed to analyse the post-mortem DNA degradation in order to use it to estimate the PMI. Studies focused mainly on animal models and on particular tissues. The results have been mixed: while on the one hand literature data in this field have confirmed that in the post-mortem several degradation processes involve nucleic acids, on the other hand some fundamental aspects are still little explored: the influence of ante and post-mortem factors on DNA degradation, the feasibility and applicability of a multiparametric mathematical model that takes into account DNA degradation and the definition of one or more target organs in order to standardize the results on human cases under standard conditions.

scholarly journals Dysregulation of miR-381-3p and miR-23b-3p in skeletal muscle could be a possible estimator of early post-mortem interval in rats

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11102
Author(s):  
Vanessa Martínez-Rivera ◽  
Christian A. Cárdenas-Monroy ◽  
Oliver Millan-Catalan ◽  
Jessica González-Corona ◽  
N. Sofia Huerta-Pacheco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
In Silico Analysis ◽  
Forensic Analysis ◽  
The Body ◽  
Control Group ◽  
Post Mortem ◽  
Rt Pcr ◽  
Post Mortem Interval ◽  
Silico Analysis

Background The post-mortem interval (PMI) is the time elapsed since the dead of an individual until the body is found, which is relevant for forensic purposes. The miRNAs regulate the expression of some genes; and due to their small size, they can better support degradation, which makes them suitable for forensic analysis. In the present work, we evaluated the gene expression of miR-381-3p, miR-23b-3p, and miR-144-3p in skeletal muscle in a murine model at the early PMI. Methods We designed a rat model to evaluate the early PMI under controlled conditions. This model consisted in 25 rats divided into five groups of rats, that correspond to the 0, 3, 6, 12 and 24 hours of PMI. The 0 h-PMI was considered as the control group. Muscle samples were taken from each rat to analyze the expression of miR-381-3p, miR-23b-3p, and miR-144-3p by quantitative RT-PCR. The gene expression of each miRNA was expressed as Fold Change (FC) and compared among groups. To find the targets of these miRNAs and the pathways where they participate, we performed an in-silico analysis. From the gene targets of miR-381-3p identified in the silico analysis, the EPC1 gene was selected for gene expression analysis by quantitative RT-PCR in these samples. Also, to evaluate if miR-381-3p could predict the early PMI, a mixed effects model was calculated using its gene expression. Results An upregulation of miR-381-3p was found at 24 h-PMI compared with the control group of 0 h-PMI and (FC = 1.02 vs. FC = 1.96; p = 0.0079). This was the opposite for miR-23b-3p, which had a down-regulation at 24 h-PMI compared to 0 h-PMI (FC = 1.22 vs. FC = 0.13; p = 0.0079). Moreover, the gene expression of miR-381-3p increased throughout the first 24 h of PMI, contrary to miR-23b-3p. The targets of these two miRNAs, participate in biological pathways related to hypoxia, apoptosis, and RNA metabolism. The gene expression of EPC1 was found downregulated at 3 and 12 h of PMI, whereas it remained unchanged at 6 h and 24 h of PMI. Using a multivariate analysis, it was possible to predict the FC of miR-381-3p of all but 6 h-PMI analyzed PMIs. Discussion The present results suggest that miR-23b-3p and miR-381-3p participate at the early PMI, probably regulating the expression of some genes related to the autolysis process as EPC1 gene. Although the miR-381-3p gene expression is a potential estimator of PMI, further studies will be required to obtain better estimates.

scholarly journals Estimation of accumulated degree hours-based post-mortem intervals in mammalian and avian model

2021 ◽  
Vol 9 (1-2) ◽  
pp. 49-58
Author(s):  
Md Golam Mostafa ◽  
Mst Shahanaz Parvin Shobha ◽  
Anjuman Ara ◽  
Saadia Ahmad
Keyword(s):  
Developmental Time ◽  
Gallus Domesticus ◽  
Post Mortem ◽  
Gallus Gallus Domesticus ◽  
Related Factors ◽  
New Approach ◽  
Post Mortem Interval ◽  
The Difference ◽  
Felis Chaus

The post-mortem interval (PMI) of a jungle cat (Felis chaus) and a chicken (Gallus gallus domesticus) were estimated as a mammalian and an avian model, respectively. The estimations were performed adopting the Accumulated Degree Hours (ADH) method using blowfly, Lucilia sericata. The required developmental time from oviposition to the 3rd instar larvae of the blowfly species was considered for the determination of PMI in each of the study. The results revealed that the calculated PMI of jungle cat was 5.29 days. On the other hand, the estimated PMI of the chicken was 2.83 days. The difference of these two PMIs in two different models might be due to the variation of temperatures and other related factors in the development of the larvae of the blowfly species. This study might offer a new approach in the medico-legal investigations with a view to resolving homicide as well as other suspected death of animals including wildlife in Bangladesh. Jahangirnagar University J. Biol. Sci. 9(1 & 2): 49-58, 2020 (June & December)

Use of entomotoxicology in estimating post-mortem interval and determining cause of death

2017 ◽  
Vol 295 ◽  
pp. 56-63
Author(s):  
Joanna Stojak ◽  
Keyword(s):  
Cause Of Death ◽  
Current Knowledge ◽  
The Body ◽  
Toxic Substances ◽  
Post Mortem ◽  
Toxicological Analysis ◽  
Post Mortem Interval ◽  
The Impact

Entomotoxicology allows the estimation of the post-mortem interval and the determination of the cause of death in cases in which the corpse has decomposed and the tissues necessary for toxicological analysis are no longer available. Obtaining information about toxic substances potentially present in the body is possible by isolation of larvae and pupae of true flies (Diptera) and/or adult forms of, e.g., beetles (Coleoptera) present on or near the corpse. This article was intended to summarize the current knowledge in the field of entomotoxicology, including examples from the literature, and to present the impact of selected toxic substances and medicines on the development of necrophagous larvae of insects.

Estimation of the post-mortem interval: effect of storage conditions on the determination of vitreous humour [K+]

2021 ◽  
Author(s):  
Mauro T Ave ◽  
Lucía Ordóñez-Mayán ◽  
Mercedes Camiña ◽  
Manuel Febrero-Bande ◽  
José Ignacio Muñoz-Barús
Keyword(s):  
Storage Conditions ◽  
Vitreous Humour ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Interval Effect

scholarly journals Individualised and non-contact post-mortem interval determination of human bodies using visible and thermal 3D imaging

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Leah S. Wilk ◽  
Gerda J. Edelman ◽  
Martin Roos ◽  
Mara Clerkx ◽  
Inge Dijkman ◽  
...  
Keyword(s):  
Estimation Method ◽  
Contact Method ◽  
Post Mortem ◽  
Correction Factors ◽  
Current Standard ◽  
Post Mortem Interval ◽  
Forensic Practice ◽  
Potential Applicability ◽  
Pmi Estimation

AbstractDetermining the time since death, i.e., post-mortem interval (PMI), often plays a key role in forensic investigations. The current standard PMI-estimation method empirically correlates rectal temperatures and PMIs, frequently necessitating subjective correction factors. To overcome this, we previously developed a thermodynamic finite-difference (TFD) algorithm, providing a rigorous method to simulate post-mortem temperatures of bodies assuming a straight posture. However, in forensic practice, bodies are often found in non-straight postures, potentially limiting applicability of this algorithm in these cases. Here, we develop an individualised approach, enabling PMI reconstruction for bodies in arbitrary postures, by combining photogrammetry and TFD modelling. Utilising thermal photogrammetry, this approach also represents the first non-contact method for PMI reconstruction. The performed lab and crime scene validations reveal PMI reconstruction accuracies of 0.26 h ± 1.38 h for true PMIs between 2 h and 35 h and total procedural durations of ~15 min. Together, these findings broaden the potential applicability of TFD-based PMI reconstruction.

Sign in / Sign up

Export Citation Format

Share Document