scholarly journals Effect of pre-sowing treatment on seed germination and seedling vigour in endive and chicory

2009 ◽  
Vol 36 (No. 3) ◽  
pp. 117-125 ◽  
Author(s):  
G. Tzortzakis N
Keyword(s):  
Seed Germination ◽  
Essential Oil ◽  
Plant Growth ◽  
Seedling Emergence ◽  
Fresh Weight ◽  
Seedling Vigour ◽  
Gibberelic Acid ◽  
Mean Germination Time ◽  
The Mean

The influence of pre-sowing treatments using hydropriming, osmopriming and halopriming in laboratory and/or nursery tests on seedling emergence, seedling weight and plant growth of endive and chicory was examined. Halopriming (KNO<sub>3</sub>) or growth regulators (gibberelic acid; GA<sub>3</sub>) improved the rate of germination of endive and chicory and reduced the mean germination time needed. 30 min pre-sowing treatment with NaHClO<sub>3</sub>, methyl jasmonate and dictamus essential oil decreased seed germination as well as seed radicle length in vitro. In the nursery tests, pre-sowing treatments had in some extent impact on the upper part (fresh weight) of the seedling, while no major changes were observed for leaf number and root fresh weight. 6-benzylaminopurine (BAP) or NaHClO<sub>3</sub> treatment reduced plant growth of both species. The present study suggests that KNO<sub>3</sub> and secondly GA<sub>3</sub> treatments may improve rapid and uniform seedling emergence and plant development in nurseries and/or in greenhouses, which is easily applicable by nursery workers with economic profits.

scholarly journals In Vitro Decoated Seed Germination and Seedling Development for Propagation of Wild Mandrake (Mandragora autumnalis Bertol.)

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1339
Author(s):  
Hani Al-Ahmad
Keyword(s):  
Seed Germination ◽  
Zygotic Embryo ◽  
Seedling Emergence ◽  
Cold Treatment ◽  
Fresh Weight ◽  
Plant Materials ◽  
System P ◽  
Double Number

The establishment of an efficient in vitro propagation system for the conservation of the Mediterranean Mandragora autumnalis is highly desirable due to its scarcity, besides its potential medicinal and pharmacological properties. In a separate unpublished study, this species has proved to be resistant to laboratory plant regeneration from vegetative tissue cultures; therefore, an alternative decoated seed (i.e., endosperm enclosed the zygotic embryo) germination approach was conducted in this study. Pre-cold treatment of M. autumnalis seeds, removal of seed coats, and exogenous application of gibberellic acid (GA3) promoted in vitro seed germination and seedling emergence. In two separate experiments, approximately 10–27% of the germinated decoated seeds developed healthy seedlings within two weeks, compared to the non-germinated intact seeds of the potting soil controls. After 72 days, the highest rates of healthy seedlings development (67.4 and 69.4%) achieved in the in vitro decoated seed cultures supplemented with 60 and 100 mg/L GA3, respectively, compared to only 25% seedlings emergence rate of the in vitro cultures devoid of GA3, and 44.2% of the soil controls. The in vitro developed plants were healthy, survived transplantation conditions, and, significantly, grew faster, formed on average more than the double number of true leaves and shoot fresh weight (p ≤ 0.05), 90% more fresh weight of root system (p ≤ 0.05), and ultimately more than the double gross fresh weight (p ≤ 0.05) than that of the in vivo developed plants of the soil controls. Such in vitro seed germination approaches would be favorable due to the higher capacity of uniform seedling establishment year-round under lab-controlled conditions, facilitating proliferation and conservation of rare and threatened species, and providing fresh and axenic plant materials required for downstream studies such as those associated with leaf-derived protoplasts and genetic transformations.

scholarly journals Effect Of Pre-Sowing Seed Treatments With Silicon Nanoparticles On Germinability Of Sunflower (Helianthus Annuus)

2015 ◽  
Vol 21 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Mohsen Janmohammadi ◽  
Naser Sabaghnia
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Crop Production ◽  
Silicon Nanoparticles ◽  
Seedling Emergence ◽  
Seedling Vigour ◽  
Seed Soaking ◽  
Positive Effects ◽  
Nano Silicon ◽  
Sowing Seed

AbstractSilicon is one of the most widespread macro elements that have beneficial effects on plant growth. Although its positive effects on plant growth and development have been widely considered, little information is available about possibility of nano-silicon utilization in seed invigoration treatments. Enhanced seed germination may lead to improved stand establishment and it can play important role in successful crop production. Partial hydration of the seeds followed by dehydration in a controlled environment often results in rapid seed germination and more uniform seedling emergence compared to untreated seeds. In the present study, the effect of seed soaking in different concentration nano-silicon solutions (0, 0.2, 0.4, 0.6, 0.8, 1 and 1.2 mM for 8 h) on germination characteristics of sunflower was investigated. Seed soaking in low concentration nano-silicon solutions (0.2 and 0.4 mM) significantly reduced days to 50% germination and mean germination time and improved root length, mean daily germination, seedling vigour index and final germination percentage. These results suggest that the incorporation of nano-silicon in priming solution, in an appropriate concentration, remarkably enhances germination performance and causes an effective invigoration of the seedling. These results underline the importance of pre-sowing seed soaking in diluted nano-silicon solutions for improving the germinability of sunflower.

scholarly journals Phytase-Producing Rahnella aquatilis JZ-GX1 Promotes Seed Germination and Growth in Corn (Zea mays L.)

2021 ◽  
Vol 9 (8) ◽  
pp. 1647
Author(s):  
Gui-E Li ◽  
Wei-Liang Kong ◽  
Xiao-Qin Wu ◽  
Shi-Bo Ma
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Biomass Accumulation ◽  
Root Surface ◽  
Phosphorus Concentration ◽  
Maize Seedlings ◽  
Total Phosphorus Concentration ◽  
Rahnella Aquatilis ◽  
Maize Seeds

Phytase plays an important role in crop seed germination and plant growth. In order to fully understand the plant growth-promoting mechanism by Rahnella aquatilis JZ-GX1，the effect of this strain on germination of maize seeds was determined in vitro, and the colonization of maize root by R. aquatilis JZ-GX1 was observed by scanning electron microscope. Different inoculum concentrations and Phytate-related soil properties were applied to investigate the effect of R. aquatilis JZ-GX1 on the growth of maize seedlings. The results showed that R. aquatilis JZ-GX1 could effectively secrete indole acetic acid and had significantly promoted seed germination and root length of maize. A large number of R. aquatilis JZ-GX1 cells colonized on the root surface, root hair and the root interior of maize. When the inoculation concentration was 107 cfu/mL and the insoluble organophosphorus compound phytate existed in the soil, the net photosynthetic rate, chlorophyll content, phytase activity secreted by roots, total phosphorus concentration and biomass accumulation of maize seedlings were the highest. In contrast, no significant effect of inoculation was found when the total P content was low or when inorganic P was sufficient in the soil. R. aquatilis JZ-GX1 promotes the growth of maize directly by secreting IAA and indirectly by secreting phytase. This work provides beneficial information for the development and application of R. aquatilis JZ-GX1 as a microbial fertilizer in the future.

scholarly journals Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

Plants ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 79 ◽  
Author(s):  
María Ibáñez ◽  
María Blázquez
Keyword(s):  
Seed Germination ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Food Crops ◽  
Potential Hazard ◽  
Spectrometry Analysis

The chemical composition of winter savory, peppermint, and anise essential oils, and in vitro and in vivo phytotoxic activity against weeds (Portulaca oleracea, Lolium multiflorum, and Echinochloa crus-galli) and food crops (maize, rice, and tomato), have been studied. Sixty-four compounds accounting for between 97.67–99.66% of the total essential oils were identified by Gas Chromatography-Mass Spectrometry analysis. Winter savory with carvacrol (43.34%) and thymol (23.20%) as the main compounds produced a total inhibitory effect against the seed germination of tested weed. Menthol (48.23%), menthone (23.33%), and iso-menthone (16.33%) from peppermint only showed total seed germination inhibition on L. multiflorum, whereas no significant effects were observed with trans-anethole (99.46%) from anise at all concentrations (0.125–1 µL/mL). Low doses of peppermint essential oil could be used as a sustainable alternative to synthetic agrochemicals to control L. multiflorum. The results corroborate that in vivo assays with a commercial emulsifiable concentrate need higher doses of the essential oils to reproduce previous in vitro trials. The higher in vivo phytotoxicity of winter savory essential oil constitutes an eco-friendly and less pernicious alternative to weed control. It is possible to achieve a greater in vivo phytotoxicity if less active essential oil like peppermint is included with other active excipients.

scholarly journals Swailing affects seed germination of plants of European bio-and agricenosis in a different way

2017 ◽  
Vol 12 (1) ◽  
pp. 62-75 ◽  
Author(s):  
Renata Bączek-Kwinta
Keyword(s):  
Seed Germination ◽  
Agricultural Practice ◽  
Seedling Vigour ◽  
Habitat Composition ◽  
Indirect Impact ◽  
Wild Thyme ◽  
Plant Habitat ◽  
Seed Testa ◽  
Stimulation Of

AbstractSwailing as a part of agricultural practice is an illegal habit in many European countries. The indirect effect of swailing is the emission of volatiles (SGV), hence the aim of the study was to identify their impact to seeds of different species occurring or grown Europe. It was carried out on seeds of 29 species of 10 botanical families within the angiosperms. The response to SGV was more or less differentiated within a family, and even within the species, e.g. in the case of tomato. The stimulation of germination and/or increased seedling vigour was established in celery, green- and red-leafed basil, white and red cabbage, white clover and wild thyme. The same effect was noticed for the seeds of stratified broadleaf plantain and the positively photoblastic seeds of German chamomile germinated in darkness. The inhibition of seed germination and/ or reduced seedling vigour was demonstrated in case of caraway, dill and forget-me-not. Similar results were obtained in the experiments carried outin vitroand in the soil, hence it can be assumed that the indirect impact of SGV on plant habitat composition is likely. The interaction of SGV compounds with seed testa and seed phytohormones is discussed.

scholarly journals In vitro Embryo Morphogenesis and Micropropagation of Dendrobium aggregatum Roxb.

2014 ◽  
Vol 23 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Mohammad Musharof Hossain
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Developmental Stages ◽  
Cell Mass ◽  
Multiple Shoot ◽  
Seedling Development ◽  
Ms Medium ◽  
Multiple Shoot Buds ◽  
Half Strength

In vitro embryo morphogenesis and micropropagation of Dendrobium aggregatum Roxb. were described. The gradual developmental stages of embryos to seedlings were traced out. Within two weeks of culture the cells of undifferented embryos underwent repeated aniclinal and periclinal division producing a compact, green parenchymatous cell mass called spherule that emerged out by rupturing the testa. The spherules subsequently differentiated into greenish protocorms were considered as typical seed germination. Germination occurred on both (MS and Phytamax (PM) medium but MS medium proved to be more efficient. The primary protocorms underwent profuse proliferation through production of secondary (2º) protocorms when transferred to different plant growth regulators (PGRs) supplemented MS; the medium fortified with 2.0 mg/l BAP and 1.0 mg/l NAA proved to be most effective for induction of 2º protocorms and seedling development. Multiple shoot buds (MSBs) were induced in pseudobulb segments of the in vitro grown seedlings when cultured on different PGRs supplemented media; and the maximum number of MSBs were obtained MS + 2.0 mg/l BAP + 0.5 mg/l picloram. The MSBs underwent elongation and then they rooted when they were transferred to half strength of MS + 0.5 mg/l IAA. The well rooted plantlets were finally transferred to outside natural environment with 80% survival. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17525 Plant Tissue Cult. & Biotech. 23(2): 241-249, 2013  (December)

Comparison of the plant growth-promotion performance of a consortium of Bacilli inoculated as endospores or as vegetative cells

2019 ◽  
Vol 95 (11) ◽  
Author(s):  
Isha Hashmi ◽  
Christophe Paul ◽  
Andrej Al-Dourobi ◽  
Frederic Sandoz ◽  
Priscilla Deschamps ◽  
...  
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Positive Impact ◽  
Growth Promotion ◽  
Soil Microbial Communities ◽  
Vegetative Cells ◽  
Single Strain ◽  
Positive Effects ◽  
Pot Experiments

ABSTRACT The effect of three plant growth-promoting Bacillus strains inoculated either alone or as a consortium was tested on oat (Avena sativa) growth. The bioinoculants were applied as vegetative cells or endospores at low cell densities on the seeds and their effect was tested in sterile in vitro conditions, pot experiments, and a field trial. The in vitro seed germination assay showed that both individual bacterial inocula and bacterial consortia had positive effects on seed germination. Greenhouse pot experiments with sterile and non-sterile soil showed that consortia increased the total dry biomass of oat plants as compared to single strain inoculation and uninoculated controls. However, the positive impact on plant growth was less prominent when the bioinoculated strains had to compete with native soil microbes. Finally, the field experiment demonstrated that the consortium of vegetative cells was more efficient in promoting oat growth than the endospore consortium and the uninoculated control. Moreover, both consortia successfully colonized the roots and the rhizosphere of oat plants, without modifying the overall structure of the autochthonous soil microbial communities.

scholarly journals Discontinuous hydration alters seed germination under stress of two populations of cactus that occur in different ecosystems in Northeast Brazil

2017 ◽  
Vol 27 (4) ◽  
pp. 292-302 ◽  
Author(s):  
Ayslan T. Lima ◽  
Marcos V. Meiado
Keyword(s):  
Seed Germination ◽  
Stress Responses ◽  
Environmental Stresses ◽  
Arid Ecosystems ◽  
Saline Stress ◽  
Mean Germination Time ◽  
The Mean ◽  
Two Populations ◽  
Hydration And Dehydration ◽  
Water And Saline Stress

AbstractThe water uptake by the seed in arid and semi-arid ecosystems may not be continuous, but may occur in hydration and dehydration cycles (HD). In order to investigate the benefits of discontinuous hydration on seed germination of Pilosocereus catingicola subsp. salvadorensis subjected to environmental stresses, an imbibition curve was determined and times X, Y and Z selected, corresponding to 25, 50 and 75% of the first phase of imbibition, respectively. Seeds of two populations (Caatinga and Restinga) were subjected to 0, 1, 2 and 3 HD cycles, with 48 h of dehydration. Water and saline stress responses were tested at five osmotic potentials (0.0, –0.1, –0.3, –0.6 and –0.9 MPa). Discontinuous hydration provided greater tolerance to water and saline stress in the seeds from the Caatinga region. When the seeds were subjected to water stress, the reduction of the mean germination time (MGT) provided by HD cycles decreased with increasing concentration of the solution. In saline stress, the reduction of MGT was greater in the solutions with the highest concentrations. We conclude that the seeds of the same species collected from populations located in different ecosystems have different germination responses after passage through discontinuous hydration and HD cycles. This provides greater tolerance to environmental stresses but with different responses among populations.

scholarly journals Rate and Synchrony of Seed Germination Influence Growth of Hydroponic Lettuce

HortScience ◽  
1999 ◽  
Vol 34 (1) ◽  
pp. 100-103
Author(s):  
Ahmet Korkmaz ◽  
Wallace G. Pill ◽  
Bruce B. Cobb
Keyword(s):  
Seed Germination ◽  
Growth Response ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Time Range ◽  
Fresh Weight ◽  
Shoot Fresh Weight ◽  
Seedling Length ◽  
Emergence Rate ◽  
Germinated Seeds

The effect of seed germination rate, or of seedling emergence rate, was studied in relation to subsequent plant growth of `Cortina' lettuce (Lactuca sativa L.). Seedling growth response to selection by time of germination was assessed by imbibing seeds at 5 °C to increase the time range for germination. Germinated seeds were removed daily and transferred to “slants” (germination paper held at 20° from vertical) at 15 °C. Five days after each transfer, root and hypocotyl lengths were measured. As days required for germination increased, root lengths decreased and hypocotyl lengths increased, resulting in no change in total seedling length. The relation between rate of seedling emergence from raw or pelleted seeds of the same lot and shoot fresh weight was examined using commercially practiced hydroponic techniques. Shoot fresh weight at 10 and 21 days after planting was related inversely and linearly to the day of emergence for both seed treatments. In the same study, the coefficient of variation of shoot fresh weight was positively related to time of seedling emergence only at 10 days. Germinated seeds were selected after 1 and 2 days of imbibition; subsequent seedling emergence rate and shoot fresh weight at 25 days were recorded. First-day germinated seeds had faster and more synchronous emergence, and produced heavier and more uniform shoots. Discarding slow-to-germinate seeds should enhance seedling emergence and growth.

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