Evaluation of entomopathogenic fungi against Raoiella indica Hirst (Acari: Prostigmata: Tenuipalpidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 219-224
Author(s):  
Indhusri Chavan ◽  
S. Pradeep ◽  
S. Sridhara ◽  
K.N. Ashrith
Keyword(s):  
Laboratory Condition ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Fungal Pathogens ◽  
Environmentally Friendly ◽  
Probit Analysis ◽  
Life Stages ◽  
Leaf Discs ◽  
Lecanicillium Lecanii ◽  
Raoiella Indica

Entomopathogenic fungi Metarrhizium anisopliae, Beauveria bassiana and Lecanicillium lecanii tested against immature and adults of Raoiella indica under laboratory condition with five different concentrations of each sprayed on leaf discs containing larvae, nymphs, and adults, indicated that all life stages were susceptible. Larval and nymphal stages were generally less susceptible than adults. Based on probit analysis, L. lecanii was the most virulent with LC50 of 8.15 × 105 conidia ml-1 and 1.30 × 105 conidia ml-1 followed by M. anisopliae 18.05 x 105 conidia ml-1 and 2.70 × 105 conidia/ml and B. bassiana (27.13 × 105 conidia ml-1 and 4.80 × 105 conidia ml-1) for immature and adults, respectively. However the efficacy of the fungal pathogens evaluated clearly differs from that of the controls. These entomopathogenic fungi could be considered as an environmentally friendly alternative for biocontrol of R. indica.

scholarly journals JAMUR ENTOMOPATOGEN ASAL TANAH LEBAK DI SUMATERA SELATAN DAN POTENSINYA SEBAGAI AGENSIA HAYATI WALANG SANGIT (LEPTOCORISA ORATORIUS (F.))

2010 ◽  
Vol 10 (2) ◽  
pp. 154-161
Author(s):  
Effendy TA ◽  
Robby Septiadi ◽  
Abdullah Salim ◽  
Abdul Mazid
Keyword(s):  
Beauveria Bassiana ◽  
Analysis Of Variance ◽  
Entomopathogenic Fungi ◽  
Probit Analysis ◽  
Biological Agents ◽  
Biocontrol Agents ◽  
Stink Bug ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Leptocorisa Oratorius

Entomopathogenic fungi from the lowland soil of South Sumatera and their potential as biocontrol agents of Stink Bug (Leptocorisa oratorius (F.)). The purpose of this research was to explore and examine the potential of soil borne entomopathogenic fungi from South Sumatera as biological agents of Leptocorisa oratorius (F).  Soil used for trapping entomopatogenic fungi, was taken from five locations  in South Sumatera.  Percentage of nymph mortality was analyzed by using analysis of variance and arranged in Completely Randomized Design.  LT50­ of nymph mortality was determined with Probit Analysis.  Entomopathogenic fungi found in this research were Beauveria bassiana and Metarhizium sp.  Stink bug mortality due to B. bassiana isolates reached 40-73.3% and Metarhizium sp. reached 56.7-70%. The results showed that Metarhizium isolates were more virulent than B. bassiana isolates.  The viability of B. bassiana conidia did not decrease during sub-culturing.  Sub-culturing did not influence the mortality of stink bug nymph but it prolonged the LT50­  of the stink bug.

scholarly journals Pathogenicity of three commercial products of entomopathogenic fungi, Beauveria bassiana, Metarhizum anisopilae and Lecanicillium lecanii against adults of olive fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) in the laboratory

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 98-102 ◽  
Author(s):  
M.F. Mahmoud
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Oral Treatment ◽  
Bactrocera Oleae ◽  
Commercial Products ◽  
Lecanicillium Lecanii ◽  
Laboratory Conditions ◽  
Olive Fly ◽  
Mortality Response ◽  
Lethal Time

The pathogenicity of entomopathogenic fungi, <I>Beauveria bassiana, Metarhizum anisopilae</I> and <I>Lecanicillium lecanii</I>, was evaluated against adults of the olive fly <I>Bactrocera oleae</I> (Gmelin) under laboratory conditions by two ways, contact bioassays and oral bioassays. The results showed that oral bioassays caused higher mortality after four treatments than the used contact bioassays. Moreover, the virulence of<I>L. lecanii</I> was higher than the virulence of <I>B. bassiana</I> and<I> M. anisopilae</I> in both ways of experiment. Lethal time (LT<sub>50</sub>) was shorter in oral bioassays than in contact bioassays in all treatments. It was 14.67, 8.30 and 5.43 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I> with oral treatment while it was 16.6, 26.07 and 12.59 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I>, respectively, with contact treatment. The slope values were 2.41, 2.55 and 2.37 for contact bioassays and 1.64, 1.69 and 1.61 for oral bioassays of <I>B. bassiana, M. anisopilae</I>and<I> L. lecanii</I>, respectively. The mortality response to the interaction between <I>B. bassiana</I> and <I>M. anisopilae</I> was synergistic while the interaction between <I>B. bassiana + L. lecanii</I> and <I>M. anisopilae + L. lecanii </I>showed an antagonistic response.

Susceptibility of different life stages of Ornithodoros lahorensis to entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana

2012 ◽  
Vol 111 (4) ◽  
pp. 1779-1783 ◽  
Author(s):  
Mousa Tavassoli ◽  
Farnaz Malekifard ◽  
Ali Soleimanzadeh ◽  
Seyed Hassan Pourseyed ◽  
Iraj Bernousi ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Life Stages

scholarly journals Biorational Management And Mycosis Studies Of Grape Thrips [Rhipiphorothrips cruentatus H.]

2021 ◽  
Author(s):  
Jawahar Ankireddy Reddy
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Methanolic Extract ◽  
Water Extract ◽  
Cost Benefit ◽  
Neem Oil ◽  
Lecanicillium Lecanii ◽  
Cost Benefit Ratio ◽  
Thrips Population

Field experiment was conducted to test the bioefficacy of various biorational insecticides against grapevine thrips at AICRP on Fruits, Department of Horticulture, MPKV, Rahuri, during 2017-18. Results revealed that standard check emamectin benzoate 5% SG consistently proved to be the most promising by recording the least thrips population (3.10/shoot). Among biorational insecticides neem oil 2% (4.09/shoot) proved as best treatment followed by, karanj oil (4.51/shoot) and neemazol (5.08/shoot). While entomopathogenic fungi Lecanicillium lecanii recorded less population (4.24/shoot) and emerged as best treatment as compared to the Metarhizium anisopliae (4.87/shoot) and Beauveria bassiana (5.34/shoot). However chlli methanolic extract (6.29/shoot), garlic methanolic extract (6.78/shoot), chilli water extract (6.85/shoot) and garlic water extract (7.08/shoot) are the least effective treatments. Incremental Cost Benefit Ratio (ICBR) in respect of different treatments ranged between 1.30 to 7.92. The highest ICBR of 1:7.92 was recorded in the treatment with emamectin benzoate 5 SG, and it was followed by Lecanicillium lecanii (1:6.34) and Metarhizium anisopliae (1:5.32). Although neem oil and karanj oil has great reduction of thrips population, but has less incremental cost benefit ratio i.e. 2.81 and 3.04, respectively, due to high dose and its cost. The mycosis test of three entomopathogenic fungi viz. Beauveria bassiana, Metarhizium anisopliae, Lecanicillium lecanii were studied on grape thrips. Mycosis by Beauveria bassiana was confirmed the pathogenicity of entomopathogenic fungi on grape thrips. Highly pronounced mycosis was observed by Metarhizium anisopliae on the dead bodies of thrips. Mycosis test of Lecanicillium lecanii was also proved on grape thrips (plate - 1, 2 and 3).

scholarly journals Laboratory evaluation of entomopathogenic fungi as biological control agents against the bark beetle Pityogenes scitus Blandford (Coleoptera: Curculionidae) in Kashmir

2018 ◽  
Vol 60 (2) ◽  
pp. 83-90
Author(s):  
Abdul L. Khanday ◽  
Abdul A. Buhroo ◽  
Avunjikkattu P. Ranjith ◽  
Sławomir Mazur
Keyword(s):  
Bark Beetle ◽  
Entomopathogenic Fungi ◽  
Bark Beetles ◽  
Fungal Pathogens ◽  
Positive Control ◽  
Laboratory Evaluation ◽  
Forest Protection ◽  
Forest Pests ◽  
Lecanicillium Lecanii ◽  
Petri Plate

Abstract The bark beetles (Coleoptera: Curculionidae) are widely recognised as one of the most damaging group of forest pests. Entomopathogenic fungi have shown great potential for the management of some bark beetle species. The efficacy of three entomopathogenic fungi, namely, Beauveria bassiana (Balsamo) Vuillemin, Metarhizium anisopliae sensu lato (Metchnikoff) Sorokin and Lecanicillium lecanii (Zimmerman) Zare and Gams was tested against the bark beetle Pityogenes scitus Blandford under the laboratory conditions. An insecticide – cyclone 505 EC, was also used as positive control in the experiment. Each fungal suspension contained 1.0×109 spores of fungi in 1 ml. In treated branches, B. bassiana and M. anisopliae caused higher percentage of mortalities, that is, 58.33% and 48%, respectively, after 10 days of treatment and 85% and 71%, respectively, after 20 days of treatment. In petri plate assay, B. bassiana, M. anisopliae and L. lecanii caused 100%, 100% and 73.33% of mortality respectively. The percentage of mortality caused by treated insecticide was 79.16%. The results obtained in the present study are promising; however, no recommendations concerning the potential use of these fungal pathogens in forest protection can be given, and further research studies are needed in this respect, especially under field conditions.

Virulence of Some Entomopathogenic Fungi Isolates of Beauveria bassiana (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Hypocreales: Clavicipitaceae) to Aulacaspis tubercularis (Hemiptera: Diaspididae)and Icerya seychellarum (Hemiptera: Monophlebidae) on Mango Crop

2019 ◽  
Author(s):  
Atef M M Sayed ◽  
Christopher A Dunlap
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Microbial Control ◽  
Probit Analysis ◽  
Scale Insects ◽  
Elapsed Time ◽  
Fungal Isolates ◽  
Further Development

Abstract Six fungal isolates of Beauveria bassiana (Balsamo) Vuillemin and one isolate of Metarhizium anisopliae (Metschnikoff) Sorokin were isolated and evaluated for their pathogenicity to Icerya seychellarum (Westwood) and Aulacaspis tubercularis Newstead. There is a positive correlation between the concentration of the fungal blastospore concentrations and the percentage of mortality. Bio-efficacy increased significantly after inoculation with increasing concentration of blastospores and elapsed time up to 12 d after inoculation. The mortality of nymphs exposed to fungal isolates at various concentrations varied between 2.5 and 88.8%. Probit analysis of data at 95% confidence limits of LC50 and LT50s showed significant differences in the susceptibility of nymphs of I. seychellarum and A. tubercularis to the tested fungal isolates. The fungal isolates of Egy-6 and Egy-9 were the most effective against I. seychellarum and A. tubercularis, respectively. They had the lowest LC50 (4.20 × 105 and 5.71 × 103 blastospore ml−1) and LT50 (ranged from 4.61 to 9.79 and 4.84 to 8.71 d), respectively. The current study showed that all the fungal isolates yielded moderate mortality rates of nymphs and adult female populations of both the tested insect pests. To our knowledge, this is the first report of bio-efficacy of Beauveria and Metarhizium isolates against members of the Diaspidadae and Monophlebidae family insects. These results establish that the use of these native entomopathogenic fungi isolates of B. bassiana (Egy-3, Egy-4, Egy-6, Egy-7, Egy-9, and Egy-10) and M. anisopliae (Egy-5) could be considered for further development as microbial control agents of the mealybug and scale insects as a potential biological agent for use in an IPM program.

scholarly journals Efficacy of biopesticides against the whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), on parthenocarpic cucumber grown under protected environment in India

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Dilip Shriram Ghongade ◽  
K. S. Sangha
Keyword(s):  
Bemisia Tabaci ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Untreated Control ◽  
Market Value ◽  
High Yield ◽  
Life Stages ◽  
Lecanicillium Lecanii ◽  
Botanical Pesticide ◽  
Chemical Groups

Abstract Background The whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is one of the most damaging pests of crops grown in open field and under protected conditions. Owing to the indiscriminate use of insecticides, whitefly has developed resistance against various insecticides belonging to different chemical groups. Use of microbial biopesticides (entomopathogenic fungi) can be an effective alternate to chemical insecticides. Results This study was planned for the evaluation of entomopathogenic fungi (EPF) formulations, namely, Beauveria bassiana Balsamo (Vuillemin), Lecanicillium lecanii (Zimmerman) Viegas and Metarhizium anisopliae (Metschnikoff) Sorokin and botanical pesticide, Neem Baan, for the management of different life stages of B. tabaci on parthenocarpic cucumber grown under protected conditions. The liquid formulations of Neem Baan at 10 and 15 ml/l were the most effective (90.7 to 93.3% in eggs, 93.3 to 97.1% in nymphs, and 92.4 to 94.2% reduction in whitefly adults after 3rd spray, respectively) as compared to EPF. Among the EPF, L. lecanii and B. bassiana at 10 and 15 ml/l (80.6 to 86.5% in eggs, 85.7 to 91.5% in nymphs, and 58.5 to 69.2% in whitefly adults after 3rd spray, respectively) were found to be more effective than M. anisopliae at 10 and 15 ml/l (78.4 to 82.8% in eggs, 82.5 to 85.9% in nymphs, and 57.7 to 62.8% in whitefly adults after third spray, respectively) in reducing different life stages of B. tabaci on cucumber. Significantly high yield of cucumber fruits was obtained from the plot where Neem Baan at 10 and 15 ml/l (2337.5 to 2420.8 g/plant) was used. Minimum fruit yield were recorded in untreated control plots. Conclusions The integration of these biopesticides in the management schedule of the whitefly under protected conditions will enhance the quality and market value of parthenocarpic cucumbers.

scholarly journals Effectiveness of Cordyceps sp. and Beauveria Bassiana Against Large Cabbage-heart Caterpillar, Crocidolomia Pavonana Fabricius (Lepidoptera: Crambidae)

2022 ◽  
Vol 16 ◽  
pp. 14-18
Author(s):  
Agus Dana Permana ◽  
Sally Marlinda ◽  
Agus Susanto ◽  
Amin Setyo Leksono
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Insect Pest ◽  
Probit Analysis ◽  
Efficacy Trial ◽  
Natural Area ◽  
Cooking Oil ◽  
Field Efficacy ◽  
Major Pest ◽  
Screen House

Crocidolomia pavonana Fabr. (Lepidoptera: Crambidae) is a major pest on various cabbage crops in Indonesia. Various insecticides have been used to control this insect pest including several entomopathogenic fungi, and this cabbage caterpillar has become resistant to various insecticides. In order to develop insecticides from entomopathogenic fungi, species or varieties from natural area such as forest are needed. A bioassays of Cordyceps sp. and Beauveria bassiana has been carried out in the laboratory. Inoculum of Cordyceps sp. obtained from the Dipterocarpaceae forest in South Kalimantan, Indonesia recovered from dead ants by the mycelium of Cordyceps sp. Four concentration of Cordyceps sp. and Beauveria bassiana inoculum and tween80 as a control were tested against 3rd instar larvae of C. pavonana with five replications. Field efficacy trial was carried out in a screen house (8 x 12 M2) using Chinese cabbage plants (Brassica juncea) aged 28 days after sowing, with a spacing of 0.4 M between rows. Three formulation of Cordyceps sp. and tween80 as a control were used with 6 replications. The result of the bioassays in the laboratory showed that Cordyceps sp. with concentration of 1 x 107 conidia mL-1 was able to give 100% mortality od C. pavonana larvae, while B. bassiana with the same concentration caused morality of C. pavonana larvae of 98.33%, although statistically not significantly different. The results of probit analysis also showed that LC90 Cordyceps sp. against cabbage caterpillar larvae was 1.57 x 106 conidia mL-1, while B. bassiana required a concentration of 2.6 x 106 conidia mL-1 to reach the LC90 value. The results of filed trial showed that the formulation of Cordyceps sp. 1 x 107 conidia mL-1 using palm oil cooking oil was able to give an average mortality of 86.7%, and significantly higher (P<0.05) than the other treatment. The results of this study indicate that the inoculum of Cordyceps sp. from Dipterocarpaceae forest is very promising to be developed as an insecticide to control C. pavonana in cabbage

scholarly journals PENGGUNAAN CENDAWAN ENTOMOPATOGEN Beauveria bassiana (BALSAMO) VUILLEMIN DAN Lecanicillium lecanii (ZIMM) ZARE & GAMS UNTUK MENGENDALIKAN Helopeltis antonii SIGN (HEMIPTERA: MIRIDAE) The Use of Entomopathogenic Fungi Beauveria bassiana (Balsamo)Vuillemin

2018 ◽  
Vol 8 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Sri Hastuti Anggarawati ◽  
Teguh Santoso ◽  
Ruly Anwar
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Low Cost ◽  
Instar Nymph ◽  
Pathology Laboratory ◽  
Tea Plantation ◽  
Lecanicillium Lecanii ◽  
Lc50 Value ◽  
Insect Pathology

Helopeltis sp. has been known as one of major pests on tea, cacao and cashew plantation. Recently, genus Helopeltis is also reported attack Acacia plantation in Sumatra and Kalimantan. For such extensive plantation, low cost biocontrol agents like entomopathogenic fungi are choosen because of the simplicity for mass production. The objective of this study was to measure the effectiveness of Beauveria bassiana and Lecanicillium lecanii against Helopeltis sp. Both fungi that were used in this study were obtained from IPB Insect Pathology Laboratory. The tested insect, Helopeltis sp. was collected from Gunung Mas tea plantation. The insects were reared in laboratory. Four level of conidial density, 109, 108, 107, 106 conidia/ mL were applied to Helopeltis adult (B. bassiana) and 3rdinstar nymph (L. lecanii). Daily mortality was observed until seven days post treatment. The result showed that L. lecanii at 106 conidia/mL caused 96.25% mortality of 3rd instar nymph of Helopeltis sp. with the LC50 value at two days observation was 1.03 x 106 conidia/ mL, LT50 was 1.198 days and LT95 was 5.25 days. On the other hand, B. bassiana at 106 conidia/ mL caused 81.25%. mortality of adult Helopeltis sp. while 100% mortality could be attained by using 108 conidia/ mL. The LC50 of B. bassiana was 3.2 x 104 conidia/ mL, at four days observation and LT50 was 4.214 days. The two fungi were thus judged effective against Helopeltis sp. in this bio assay.Key words: Beauveria bassiana, conidia, density, Helopeltis sp., Lecanicillium lecanii, mortality.

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