scholarly journals PENGGUNAAN CENDAWAN ENTOMOPATOGEN Beauveria bassiana (BALSAMO) VUILLEMIN DAN Lecanicillium lecanii (ZIMM) ZARE & GAMS UNTUK MENGENDALIKAN Helopeltis antonii SIGN (HEMIPTERA: MIRIDAE) The Use of Entomopathogenic Fungi Beauveria bassiana (Balsamo)Vuillemin

2018 ◽  
Vol 8 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Sri Hastuti Anggarawati ◽  
Teguh Santoso ◽  
Ruly Anwar
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Low Cost ◽  
Instar Nymph ◽  
Pathology Laboratory ◽  
Tea Plantation ◽  
Lecanicillium Lecanii ◽  
Lc50 Value ◽  
Insect Pathology

Helopeltis sp. has been known as one of major pests on tea, cacao and cashew plantation. Recently, genus Helopeltis is also reported attack Acacia plantation in Sumatra and Kalimantan. For such extensive plantation, low cost biocontrol agents like entomopathogenic fungi are choosen because of the simplicity for mass production. The objective of this study was to measure the effectiveness of Beauveria bassiana and Lecanicillium lecanii against Helopeltis sp. Both fungi that were used in this study were obtained from IPB Insect Pathology Laboratory. The tested insect, Helopeltis sp. was collected from Gunung Mas tea plantation. The insects were reared in laboratory. Four level of conidial density, 109, 108, 107, 106 conidia/ mL were applied to Helopeltis adult (B. bassiana) and 3rdinstar nymph (L. lecanii). Daily mortality was observed until seven days post treatment. The result showed that L. lecanii at 106 conidia/mL caused 96.25% mortality of 3rd instar nymph of Helopeltis sp. with the LC50 value at two days observation was 1.03 x 106 conidia/ mL, LT50 was 1.198 days and LT95 was 5.25 days. On the other hand, B. bassiana at 106 conidia/ mL caused 81.25%. mortality of adult Helopeltis sp. while 100% mortality could be attained by using 108 conidia/ mL. The LC50 of B. bassiana was 3.2 x 104 conidia/ mL, at four days observation and LT50 was 4.214 days. The two fungi were thus judged effective against Helopeltis sp. in this bio assay.Key words: Beauveria bassiana, conidia, density, Helopeltis sp., Lecanicillium lecanii, mortality.

scholarly journals The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity

2017 ◽  
Author(s):  
Xiang Meng ◽  
Junjie Hu ◽  
Gecheng Ouyang
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Southern China ◽  
Pathogenic Fungus ◽  
Lethal Effect ◽  
Stink Bug ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Rdna Its ◽  
Major Pest

Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.

scholarly journals Pathogenicity of three commercial products of entomopathogenic fungi, Beauveria bassiana, Metarhizum anisopilae and Lecanicillium lecanii against adults of olive fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) in the laboratory

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 98-102 ◽  
Author(s):  
M.F. Mahmoud
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Oral Treatment ◽  
Bactrocera Oleae ◽  
Commercial Products ◽  
Lecanicillium Lecanii ◽  
Laboratory Conditions ◽  
Olive Fly ◽  
Mortality Response ◽  
Lethal Time

The pathogenicity of entomopathogenic fungi, <I>Beauveria bassiana, Metarhizum anisopilae</I> and <I>Lecanicillium lecanii</I>, was evaluated against adults of the olive fly <I>Bactrocera oleae</I> (Gmelin) under laboratory conditions by two ways, contact bioassays and oral bioassays. The results showed that oral bioassays caused higher mortality after four treatments than the used contact bioassays. Moreover, the virulence of<I>L. lecanii</I> was higher than the virulence of <I>B. bassiana</I> and<I> M. anisopilae</I> in both ways of experiment. Lethal time (LT<sub>50</sub>) was shorter in oral bioassays than in contact bioassays in all treatments. It was 14.67, 8.30 and 5.43 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I> with oral treatment while it was 16.6, 26.07 and 12.59 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I>, respectively, with contact treatment. The slope values were 2.41, 2.55 and 2.37 for contact bioassays and 1.64, 1.69 and 1.61 for oral bioassays of <I>B. bassiana, M. anisopilae</I>and<I> L. lecanii</I>, respectively. The mortality response to the interaction between <I>B. bassiana</I> and <I>M. anisopilae</I> was synergistic while the interaction between <I>B. bassiana + L. lecanii</I> and <I>M. anisopilae + L. lecanii </I>showed an antagonistic response.

scholarly journals Biorational Management And Mycosis Studies Of Grape Thrips [Rhipiphorothrips cruentatus H.]

2021 ◽  
Author(s):  
Jawahar Ankireddy Reddy
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Methanolic Extract ◽  
Water Extract ◽  
Cost Benefit ◽  
Neem Oil ◽  
Lecanicillium Lecanii ◽  
Cost Benefit Ratio ◽  
Thrips Population

Field experiment was conducted to test the bioefficacy of various biorational insecticides against grapevine thrips at AICRP on Fruits, Department of Horticulture, MPKV, Rahuri, during 2017-18. Results revealed that standard check emamectin benzoate 5% SG consistently proved to be the most promising by recording the least thrips population (3.10/shoot). Among biorational insecticides neem oil 2% (4.09/shoot) proved as best treatment followed by, karanj oil (4.51/shoot) and neemazol (5.08/shoot). While entomopathogenic fungi Lecanicillium lecanii recorded less population (4.24/shoot) and emerged as best treatment as compared to the Metarhizium anisopliae (4.87/shoot) and Beauveria bassiana (5.34/shoot). However chlli methanolic extract (6.29/shoot), garlic methanolic extract (6.78/shoot), chilli water extract (6.85/shoot) and garlic water extract (7.08/shoot) are the least effective treatments. Incremental Cost Benefit Ratio (ICBR) in respect of different treatments ranged between 1.30 to 7.92. The highest ICBR of 1:7.92 was recorded in the treatment with emamectin benzoate 5 SG, and it was followed by Lecanicillium lecanii (1:6.34) and Metarhizium anisopliae (1:5.32). Although neem oil and karanj oil has great reduction of thrips population, but has less incremental cost benefit ratio i.e. 2.81 and 3.04, respectively, due to high dose and its cost. The mycosis test of three entomopathogenic fungi viz. Beauveria bassiana, Metarhizium anisopliae, Lecanicillium lecanii were studied on grape thrips. Mycosis by Beauveria bassiana was confirmed the pathogenicity of entomopathogenic fungi on grape thrips. Highly pronounced mycosis was observed by Metarhizium anisopliae on the dead bodies of thrips. Mycosis test of Lecanicillium lecanii was also proved on grape thrips (plate - 1, 2 and 3).

scholarly journals Pengaruh Komposisi Media Terhadap Produksi Konidia Jamur Entomopatogen Beauveria bassiana (Balsamo) Vuillemin

2016 ◽  
Vol 2 (2) ◽  
pp. 88
Author(s):  
I.G.A.A. Indrayani ◽  
Heri Prabowo
Keyword(s):  
Beauveria Bassiana ◽  
Solid Medium ◽  
Medium Composition ◽  
Pathology Laboratory ◽  
Glucose Addition ◽  
Insect Pathology ◽  
Average Production ◽  
Conidia Production ◽  
Speed Up ◽  
Rice Medium

<p>Penelitian pengaruh komposisisi media dan suhu terhadap produksi konidia Beauveria bassiana dilaksanakan di Laboratorium Patologi Serangga Balai Penelitian Tanaman Tembakau dan Serat, Malang mulai Mei sampai dengan November 2009. Tujuannya adalah untuk mengetahui pengaruh perbedaan komposisi media terha-dap produksi konidia B. bassiana. Perlakuan yang digunakan adalah: (1) beras, (2) jagung, (3) beras+glu-kosa, (4) beras+yeast, (5) jagung+glukosa, dan (6) jagung+yeast. Setiap perlakuan disusun dalam rancang-an acak lengkap (RAL) dengan enam kali ulangan. Parameter yang diamati adalah laju pertumbuhan dan produksi konidia. Hasil penelitian menunjukkan bahwa beras dan jagung berpotensi menjadi media tumbuh yang baik bagi jamur B. bassiana. Proses pembentukan konidia sudah dimulai pada 3 minggu setelah inoku-lasi, lebih cepat dibanding pada media jagung (4,5 minggu). Penambahan yeast lebih nyata meningkatkan produksi konidia B. bassiana pada beras maupun jagung dibanding dengan penambahan glukosa. Rata-rata produksi konidia pada komposisi media beras+yeast dapat mencapai &gt; 4,0 x 109 konidia/g media dan lebih tinggi dibanding produksi konidia pada media lainnya (&lt; 3,0 x 109 konidia/g media).</p><p> </p><p>Study on the effects of solid medium composition and temperature on conidia production of B. bassiana was conducted at Insect Pathology Laboratory of Indonesian Tobacco and Fiber Crops Institute (IToFCRI), Malang from May to November 2009. The objective of the study was to find out the effects of different me-dium composition. Treatments used were (1) whole rice, (2) broken maize, (3) whole rice+glucose, (4) whole rice+yeast, (5) broken maize+glucose, and (6) broken maize+yeast. Each treatment was arranged in randomized complete design (RCD) with six replications. Parameters observed were growth rate and conidial production of B. bassiana at room temperature. Results showed that whole rice and broken maize could be used as solid medium for B. bassiana. Conidia production was faster on most of medium based on whole rice than that on broken maize. On whole rice medium conidia of B. bassiana were produced about 10 days earlier than that on broken maize. Yeast and glucose addition into medium was able to speed up the fungi growth and to increase conidia production. Addition of yeast potentialy increased conidia production com-pared with other medium with glucose addition. Average production of conidia on whole rice+yeast was more than 4,0 x 109 conidia/g medium compared with any other medium composition that produced less than 3,0 x 109 conidia/g medium.</p>

scholarly journals The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity

2017 ◽  
Author(s):  
Xiang Meng ◽  
Junjie Hu ◽  
Gecheng Ouyang
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Southern China ◽  
Pathogenic Fungus ◽  
Lethal Effect ◽  
Stink Bug ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Rdna Its ◽  
Major Pest

Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.

Evaluation of entomopathogenic fungi against Raoiella indica Hirst (Acari: Prostigmata: Tenuipalpidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 219-224
Author(s):  
Indhusri Chavan ◽  
S. Pradeep ◽  
S. Sridhara ◽  
K.N. Ashrith
Keyword(s):  
Laboratory Condition ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Fungal Pathogens ◽  
Environmentally Friendly ◽  
Probit Analysis ◽  
Life Stages ◽  
Leaf Discs ◽  
Lecanicillium Lecanii ◽  
Raoiella Indica

Entomopathogenic fungi Metarrhizium anisopliae, Beauveria bassiana and Lecanicillium lecanii tested against immature and adults of Raoiella indica under laboratory condition with five different concentrations of each sprayed on leaf discs containing larvae, nymphs, and adults, indicated that all life stages were susceptible. Larval and nymphal stages were generally less susceptible than adults. Based on probit analysis, L. lecanii was the most virulent with LC50 of 8.15 × 105 conidia ml-1 and 1.30 × 105 conidia ml-1 followed by M. anisopliae 18.05 x 105 conidia ml-1 and 2.70 × 105 conidia/ml and B. bassiana (27.13 × 105 conidia ml-1 and 4.80 × 105 conidia ml-1) for immature and adults, respectively. However the efficacy of the fungal pathogens evaluated clearly differs from that of the controls. These entomopathogenic fungi could be considered as an environmentally friendly alternative for biocontrol of R. indica.

scholarly journals Alternative substrates for conidiogenesis of the entomopathogenic fungus Beauveria bassiana (Bals) Vuillemin (Deuteromycotina: Hyphomycetes)

2020 ◽  
Vol 80 (1) ◽  
pp. 133-141
Author(s):  
A. G. L. Paiva-Guimarães ◽  
K. R. L. Freire ◽  
S. F. M. Santos ◽  
A. F. Almeida ◽  
A.C.B. Sousa
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Insecticidal Activity ◽  
Insect Pests ◽  
Low Cost ◽  
Nutritional Supplement ◽  
Conidia Production ◽  
Fungus Beauveria Bassiana ◽  
Potential Use

Abstract Beauveria bassiana is a promising fungus for the biological control of insect pests. The growing costs of conidia production have raised the need to ascertain the efficiency of some low cost substrates. The aim of this study was to analyze the potential use of different raw substrates without nutritional supplement for B. bassiana conidiogenesis. Growth and sporulation were evaluated using 30 g of substrate and 0.3 μL of a conidia suspension (1 x 106 conidia/mL). After 10 days of incubation (70 ± 10% humidity and temperature (T) = 29 ± 1 °C), rice (2.00 x 106 conidia/g substrate), algaroba (2.36 x 106 conidia/g), malt A (1.22 x 106 conidia/g) and malt B (1.75 x 106 conidia/g) showed the highest levels of conidia production. The resulting conidia showed insecticidal activity higher than 80% on coconut termites. These new raw substrates may represent viable alternatives for the production of entomopathogenic fungi for use in the biological control of various insect pests.

scholarly journals Entomopathogenic Fungal Screening against Two Spotted Spider Mites, Tetranychus urticae Koch in Tomato and Broad Mite, Polyphagotarsonemus latus (Banks) in Chilli

2021 ◽  
Author(s):  
S. Sumaiya Parveen ◽  
K. Ramaraju ◽  
S. Jeyarani
Keyword(s):  
Tetranychus Urticae ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Spider Mites ◽  
Natural Occurrence ◽  
Polyphagotarsonemus Latus ◽  
Lc50 Value ◽  
Fungal Entomopathogens ◽  
Broad Mite ◽  
Tetranychus Urticae Koch

Background: Entomopathogenic fungi are microorganisms that specifically infect and often kill insects and other arthropods. Most are non-pathogenic to plants and relatively non-toxic to humans and animals. Use of fungal entomopathogens can be an alternative to insecticides and could help in bio-intensive pest management. The current study aimed to study the pathogenicity of entomopathogenic fungal isolates against two Spotted Spider Mites, Tetranychus urticae Koch in tomato and broad Mite, Polyphagotarsonemus latus (Banks) in chilli. Methods: An extensive survey for the natural occurrence of entomopathogenic fungi on insects and mites was made in different agro climatic regions of Tamil Nadu during 2013 and 2014. Survey revealed the occurrence of two entomopathogenic fungi viz., Beauveria bassiana (Balsamo) Vuillemin and Metarhizium flavoviride Gams and Rozsypal var. minus. Laboratory experiment was conducted to evaluate the pathogenicity of fourteen isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii against Tetranychus urticae and Polyphagotarsonemus latus. Result: In opposition to T. urticae, the Beauveria bassiana isolate Bb 112 showed the highest toxicity of all the other isolates tested, with low LC50 value of 0.6 x106 spores ml-1 and LT50 of 92.60h. Against P. latus, Bb 112 had high virulence with low LC50 value of 0.3x106 spores ml-1 and LT50 value of 92.32h. Hence, there is potential for use of entomopathogenic fungi in the field conditions as an alternate control method in combating the insect pests and other arthropod pests since they are considered natural mortality agents and are environmentally safe.

scholarly journals Effect of the native strain of the predator Nesidiocoris tenuis Reuter and the entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium against Bemisia tabaci (Genn.) under greenhouse conditions in Tunisia

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Besma Hamrouni Assadi ◽  
Sabrine Chouikhi ◽  
Refki Ettaib ◽  
Naima Boughalleb M’hamdi ◽  
Mohamed Sadok Belkadhi
Keyword(s):  
Beauveria Bassiana ◽  
Bemisia Tabaci ◽  
Entomopathogenic Fungi ◽  
Tobacco Plant ◽  
Economic Losses ◽  
Native Strain ◽  
Lecanicillium Muscarium ◽  
Southern Tunisia ◽  
Significant Efficacy ◽  
Nesidiocoris Tenuis

Abstract Background The misuse of chemical insecticides has developed the phenomenon of habituation in the whitefly Bemisia tabaci (Gennadius) causing enormous economic losses under geothermal greenhouses in southern Tunisia. Results In order to develop means of biological control appropriate to the conditions of southern Tunisia, the efficacy of the native strain of the predator Nesidiocoris tenuis Reuter (Hemiptera: Miridae) and two entomopathogenic fungi (EPF) Beauveria bassiana and Lecanicillium muscarium was tested against Bemisia tabaci (Gennadius). Indeed, the introduction of N. tenuis in doses of 1, 2, 3, or 4 nymphs per tobacco plant infested by the whitefly led to highly significant reduction in the population of B. tabaci, than the control devoid of predator. The efficacy of N. tenuis was very high against nymphs and adults of B. tabaci at all doses per plant with a rate of 98%. Likewise, B. bassiana and L. muscarium, compared to an untreated control, showed a very significant efficacy against larvae and adults of B. tabaci. In addition, the number of live nymphs of N. tenuis treated directly or introduced on nymphs of B. tabaci treated with the EPF remained relatively high, exceeding 24.8 nymphs per cage compared to the control (28.6). Conclusions It can be concluded that the native strain of N. tenuis and the EPF tested separately were effective against B. tabaci. Their combined use appears to be possible.

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