scholarly journals The Effect of Biostimulators and Indole-3-Butyric Acid on Rooting of Stem Cuttings of Two Ground Cover Roses

2020 ◽  
Vol 73 (1) ◽  
Author(s):  
Andrzej Pacholczak ◽  
Karolina Nowakowska
Keyword(s):  
Butyric Acid ◽  
Free Amino ◽  
Water Solution ◽  
Soluble Sugars ◽  
Ground Cover ◽  
Stem Cuttings ◽  
Synthetic Auxin ◽  
Rooting Of Cuttings ◽  
Amino Acid Contents

One of the important groups of roses are ground cover roses, used not only as garden plants but also in urban green areas for covering scarps and hill slopes. Roses are mostly propagated in vitro and by budding, grafting, and cuttings however, the latter is feasible only in certain rose groups. To hasten the production of plant material, preparations that stimulate rhizogenesis are used, which are based mainly on auxins and enhance the growth of adventitious roots in cuttings. Other substances capable of stimulating plant growth are biostimulators, which can be an alternative to rooting formulations containing auxins. The goal of this experiment was to compare the effects of the biostimulators AlgaminoPlant and Goteo with that of the synthetic auxin indole-3-butyric acid (IBA) on rooting of cuttings in two ground cover roses Elfrid (‘Kormuse’) and Weisse Immensee (‘Korweirim’). In the preparations, IBA in the form of Rhizopon (1% IBA) or water solution (200 mg L<sup>−1</sup>) was used, whereas the biostimulators were applied at the concentration of 0.2% for foliage spraying. Both biostimulators positively affected the percentage of rooted cuttings, whereas their effect on the degree of rooting was comparable or slightly weaker than that of synthetic auxin. Both biopreparations stimulated the growth of new shoots in cuttings. The use of Goteo resulted in increased content of chlorophyll and total soluble sugars in cuttings, whereas levels of free amino acids and polyphenolic acids were decreased. The synthetic auxin IBA increased the total sugar concentration and the free amino acid contents in cuttings were decreased.

scholarly journals   Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi
Keyword(s):  
Butyric Acid ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Practical Application ◽  
Rooting Of Cuttings ◽  
Highbush Blueberries ◽  
Set Up ◽  
Indole 3 Acetic Acid

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry. &nbsp;

scholarly journals The effect of the biostimulator Goteo on the rooting of ninebark stem cuttings

2016 ◽  
Vol 28 (2) ◽  
pp. 109-116 ◽  
Author(s):  
Andrzej Pacholczak ◽  
Karolina Nowakowska ◽  
Natalia Mika ◽  
Monika Borkowska
Keyword(s):  
Free Amino ◽  
Soluble Sugars ◽  
Mechanisms Of Action ◽  
Stem Cuttings ◽  
Indole Derivatives ◽  
Synthetic Auxin ◽  
Synthetic Auxins ◽  
Nursery Production ◽  
Physocarpus Opulifolius ◽  
Insight Into

Abstract As a consequence of restrictions on the use of preparations containing synthetic auxins in nursery production, there is a necessity to replace them with more environmentally friendly biopreparations efficiently stimulating plant growth. The aim of the presented experiment was to compare the effects of the synthetic auxin indole-3-butyric acid (IBA) and the biostimulator Goteo on the rooting of ninebark stem cuttings (Physocarpus opulifolius ‘Dart’s Gold’ and ‘Red Baron’) and to get some insight into the latter’s mechanisms of action in plants. Applications of the biostimulator Goteo produced comparable or slightly weaker effects compared to the treatments with IBA. Goteo stimulated elongation in new growth of cuttings when applied in watering or two-fold spraying methods. Application of the biostimulator resulted in increased levels of chlorophyll, soluble sugars and indole derivatives, while the contents of free amino acids and polyphenolic acids decreased. The above results indicate that, if necessary, Goteo may replace the synthetic auxin IBA in the propagation of ninebark in the future.

scholarly journals Comparison of Rootability of Stem Cuttings from Seedlings of Aesculus sp. and Mature Aesculus × arnoldiana ‘Autumn Splendor’

1988 ◽  
Vol 6 (2) ◽  
pp. 69-71
Author(s):  
B.A. Bergmann ◽  
W.P. Hackett ◽  
H. Pellett
Keyword(s):  
Butyric Acid ◽  
Developmental Stages ◽  
Stem Cuttings ◽  
Mature Tree ◽  
Mature Trees ◽  
Old Growth ◽  
Rooting Of Cuttings ◽  
Root Suckers ◽  
Stem Segments

Rootability was evaluated for stem cuttings taken at varying positions and developmental stages from seedlings of Aesculus and from the mature trees of Aesculus × arnoldiana ‘Autumn Splendor’. Rooting was 100% for cuttings taken from 2-week-old seedlings and 0% for those obtained from the crown of the mature tree 2 months after commencement of spring growth flush. Intermediate rootability was found for cuttings taken from 1-month-old seedlings and from the 2 week-old growth of suckers at the base of the mature tree. Rooting of cuttings with intermediate rooting potential was increased by a quick dip treatment with 2500 ppm indole-3-butyric acid (IBA) or by using apical rather than basal stem segments. Commercially acceptable rooting was not achieved using cuttings from root suckers or the crown of Aesculus ‘Augumn Splendor’.

scholarly journals Three Mussaenda Cultivars Propagated by Stem Cuttings Exhibit Variation in Rooting in Response to Hormone and Rooting Conditions

2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Clonal Propagation ◽  
Stem Cuttings ◽  
Root Number ◽  
Root Numbers ◽  
Rooting Medium ◽  
Rooting Of Cuttings

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.

scholarly journals Physiological Aspects in Propagation of Ninebark (Physocarpus opulifolius Maxim. ‘Red Baron’) by Stem Cuttings Treated with Auxin or Biostimulator

2016 ◽  
Vol 44 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Andrzej PACHOLCZAK ◽  
Karolina NOWAKOWSKA ◽  
Stefan PIETKIEWICZ
Keyword(s):  
Plant Protection ◽  
Water Solution ◽  
Soluble Sugars ◽  
Stem Cuttings ◽  
Indole Derivatives ◽  
Total Soluble Sugars ◽  
Chlorophyll Contents ◽  
Nursery Production ◽  
Physocarpus Opulifolius ◽  
By Products

Recently, a trend can be observed to replace plant protection chemicals in nursery production by products of natural origin. Such products increase plants’ resistance to stressful conditions and lower their susceptibility to pathogens. They may also offer an alternative to chemicals stimulating rhizogenesis. The aim of this work was to evaluate the effects of foliar applications of AlgaminoPlant, a biostimulator based on a seaweed extract, relative to auxin-containing preparations, on root growth in stem cuttings of common ninebark (Physocarpus opulifolius). Cuttings were sprayed with water solution of IBA or AlgaminoPlant once, twice or three times during the rooting period at one week intervals and powder IBA was also applied directly to the bases of cuttings. The best rooting was observed after powder IBA application and satisfactory results were observed for AlgaminoPlant and aqueous IBA solution. Treatments with AlgaminoPlant and IBA increased photosynthetic efficiency, chlorophyll contents, total soluble sugars, soluble proteins, indole derivatives and catalase activity while the hydrogen peroxide level and peroxidase activity were reduced. The effects of AlgaminoPlant on rhizogenesis were comparable to synthetic IBA, or only slightly lower. Hence, if needed, synthetic auxin IBA may be replaced by AlgaminoPlant which is considered an environmentally friendly product.

scholarly journals Effect of different concentrations of indole butyric acid, putrescine and hydrogen peroxide on stem cuttings of the rootstock GF677(Prunus amygdalus × Prunus persica) according to the cutting season

2021 ◽  
Vol 74 (2) ◽  
Author(s):  
Sina Kordzadeh ◽  
Hassan Sarikhani
Keyword(s):  
Hydrogen Peroxide ◽  
Butyric Acid ◽  
Interspecific Hybrid ◽  
Prunus Persica ◽  
Callus Formation ◽  
Stem Cuttings ◽  
Prunus Amygdalus ◽  
Fresh Weight ◽  
Indole Butyric Acid ◽  
Rooting Of Cuttings

The rootstock GF677 is an interspecific hybrid with an important economic and horticultural value. In this research, the effect of indole butyric acid (IBA) in combination with putrescine (Put) and hydrogen peroxide (H2O2) on rooting of GF677 semi-hardwood stem cuttings in three cutting seasons (July, March and October) was investigated. Treatments as IBA (0, 1000, 2000 and 3000 mg L-1), Put (0, 800, 1600 and 3200 mg L-1) and H2O2 (1.5, 3 and 6% w/v) were included. The results showed that in July cuttings, the highest levels of callogenesis were observed in IBA treated cuttings in both concentrations of 1000 and 2000 mg L-1. The rooting was very low in July cuttings, while the highest percentage of rooting (14%) was observed in the combination of 2000 mg L-1 IBA+ 3% H2O2. In March, the cuttings treated by 1000 mg L-1 IBA+800 mg L-1 Put and 1000 mg L-1 IBA+1600 mg L-1 Put revealed the highest percentages of callus formation 83.31 and 83.33%, respectively. In these cuttings, the highest percentage of rooting (63.88%) was gained at 2000 mg L-1 IBA+3200 mg L-1 Put. The application of 1000 mg L-1 IBA+800 mg L-1 Put increased root fresh weight. In cuttings prepared in October, only 800 mg L-1 Put caused callus formation in more than 55% of the cuttings. The rooting of cuttings at this time was as low as the July cuttings, whereas the highest rooting percentage was observed in cuttings treated with IBA at a concentration of 1000 mg L-1. Overall, the experiment showed that the season of the cutting and the treatments with IBA+Put or H2O2 could improve rooting properties of the rootstock GF677.

scholarly journals The Effect of Brassinosteroids on Rootting of Stem Cuttings in Two Barberry (Berberis thunbergii L.) Cultivars

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 699
Author(s):  
Andrzej Pacholczak ◽  
Małgorzata Zajączkowska ◽  
Karolina Nowakowska
Keyword(s):  
Amino Acids ◽  
Hydrogen Peroxide ◽  
Plant Growth ◽  
Free Amino ◽  
Soluble Sugars ◽  
Growth Control ◽  
Stem Cuttings ◽  
Berberis Thunbergii ◽  
Higher Plant ◽  
Total Soluble Sugars

Brassinosteroids are a group of over seventy steroid compounds whose discovery in lower and higher plant organisms created new possibilities of plant growth control. The aim of the work was to evaluate the effect of two brassinosteroids: brassinolide (BL) and 24-epibrassinolide (24epiBL) as compared to the auxin rooting enhancer indole-3-butyric acid (IBA), on the rooting of stem cuttings in two Thunberg’s barberry cultivars ‘Maria’ and ‘Red Rocket’. The cuttings were sprayed with water solutions of growth regulators: IBA (200 mg·L−1), 0.05% BL or 24epiBL, as well as with a combination of each of brassinosteroids with the auxin while the control cuttings were sprayed with water. In both cultivars brassinosteroids positively affected a degree of rooting and root length. Their application resulted in elevated contents of chlorophyll, total soluble sugars, free amino acids, hydrogen peroxide and catalase activity. Brassinosteroids were more effective when combined with the auxin than when used singly.

scholarly journals Responses to Propagation Substrate and Rooting Hormone Products to Facilitate Asexual Propagation of Silene chalcedonica and Silene coronaria

2016 ◽  
Vol 34 (3) ◽  
pp. 80-83
Author(s):  
Li Jiang ◽  
Bruce L. Dunn ◽  
Yun-wen Wang ◽  
Carla L. Goad
Keyword(s):  
Butyric Acid ◽  
Cost Effective ◽  
Adventitious Rooting ◽  
Stem Cuttings ◽  
Genetic Purity ◽  
Asexual Propagation ◽  
Cutting Propagation ◽  
Auxin Treatment ◽  
Napthaleneacetic Acid ◽  
Rooting Of Cuttings

Many ornamentals are commercially propagated by cuttings, which is an easy and cost-effective propagation method. Silene L. is a promising genus for extensive use as a landscape ornamental due to its drought tolerance, profuse flowering, and perennial characteristic. Silene is readily cross pollinated, so to ensure genetic purity for breeding and cultivar release, an asexual cutting propagation protocol needs to be established. Adventitious rooting of cuttings of Silene chalcedonica (L.) E.H.L. Krause and Silene coronaria (L.) Clairv. were evaluated using two different propagation substrates and three different root-promoting hormone products. Rooting percentage of S. chalcedonica cuttings was improved by Dip ‘N’ Grow treatment (1,000 mg·L−1), which contains 1% indole-3-butyric acid and 0.5% 1-napthaleneacetic acid, and Hormex Rooting Powder No. 1 (1,000 mg·L−1), which contains 0.10% indole-3-butyric acid; however, 89% of S. coronaria cuttings rooted readily in vermiculite without an auxin treatment. Therefore, rooting success of stem cuttings was highly species related. Because of the relatively low rooting percentage seen in vermiculite (46%) and in perlite (63%) using Dip ’N Grow auxin treatment for S. chalcedonica, an effective stem cutting propagation method still needs to be explored for this species.

The effect of steaming on the free amino acid contents and antioxidant activity of ginseng

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
EJ Cho ◽  
XL Piao ◽  
MH Jang ◽  
SY Park ◽  
SW Kwon ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Amino Acid Contents

COMPARATIVE STUDIES OF THE POTENTIATION OF CORTICOTROPHIN ACTIVITY BY SEVERAL INACTIVE DERIVATIVES

1963 ◽  
Vol 42 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Arthur I. Cohen ◽  
Edward H. Frieden
Keyword(s):  
Biological Activity ◽  
Comparative Studies ◽  
Free Amino ◽  
Hormonal Activity ◽  
Amino Groups

ABSTRACT A number of corticotrophin analogues have been prepared, some of which potentiate the biological activity of the untreated hormone in vitro. The free amino groups of corticotrophin appear to be essential not only for hormonal activity, but also for the interaction of the analogues with the tissue corticotrophin inactivating system which is assumed to account for the potentiating effect.

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