scholarly journals The development and validation of new DNA markers linked to the thousand-grain weight QTL in bread wheat (Triticum aestivum L.)

2020 ◽  
Vol 56 (No. 2) ◽  
pp. 52-61
Author(s):  
Cong Cong Yang ◽  
Jian Ma ◽  
Cong Li ◽  
Min Sun ◽  
Ya Ya Zou ◽  
...  
Keyword(s):  
Dna Markers ◽  
High Resolution Melting ◽  
Triticum Aestivum L ◽  
Grain Weight ◽  
Scar Markers ◽  
Thousand Grain Weight ◽  
Marker Assisted Breeding ◽  
Positive Allele ◽  
Important Trait ◽  
Development And Validation

Thousand-grain weight (TGW) is an important trait affecting wheat production. We previously identified a major quantitative trait loci (QTL) controlling the TGW on the 2D chromosome of wheat using a recombinant inbred line (RIL) population constructed by the cross between Tibetan semi-wild wheat Q1028 (Q1028) and Zhengmai 9023 (ZM9023). The positive allele at this QTL is from ZM9023. To further characterise this QTL, here, we try to develop and validate the high-resolution melting (HRM) and sequence-characterised amplified region (SCAR) markers. One HRM marker (0C98-411) and two SCAR markers (E301-700 and B0BB-10470) were developed and integrated into the genetic map. All of these three markers were validated in three populations with different genetic backgrounds. 0C98-411 is the most closely linked marker that could trace QTgw.sau-2D in molecular marker assisted breeding.

scholarly journals Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

2019 ◽  
Author(s):  
Shenghui Zhou ◽  
Jinpeng Zhang ◽  
Haiming Han ◽  
Jing Zhang ◽  
Ma Huihui ◽  
...  
Keyword(s):  
Single Molecule ◽  
Sequence Data ◽  
Triticum Aestivum L ◽  
Full Length ◽  
Grain Weight ◽  
Agropyron Cristatum ◽  
Translocation Line ◽  
Thousand Grain Weight ◽  
Sequencing Platform ◽  
Transcriptome Sequences

Abstract Background Agropyron cristatum (L.) Gaertn. (2n = 4x = 28; genomes PPPP) is a wild relative of common wheat (Triticum aestivum L.) and provides many desirable genetic resources of wheat improvement. However, there is still a lack of reference genome and transcriptome information for A. cristatum, which severely impedes functional and molecular breeding studies. Results Single-molecule long-read sequencing technology from Pacific Biosciences (PacBio) was used to sequence full-length cDNA from a mixture of leaves, roots, stems and caryopses and constructed the first full-length transcriptome dataset of A. cristatum, which comprised 44,372 transcripts. As expected, the PacBio transcripts were generally longer than the transcripts assembled via the Illumina sequencing platform in a previous study. By mapping the full-length transcripts in this study, we identified tissue-enriched expressed transcripts and enriched GO terms, indicating that tissue-enriched transcripts were enriched for particular molecular functions that varies with tissue. We identified 3,398 novel and 1,352 A. cristatum-specific transcripts compared with the wheat gene model set. In order to better apply this transcriptome of A. cristatum, the A. cristatum transcripts was integrated with wheat genome as a reference sequences to try to identify candidate A. cristatum transcripts associated with thousand-grain weight in a wheat-A. cristatum translocation line Pubing 3035. Conclusions Full-length transcriptome sequences were used in our study. The present study not only provides comprehensive transcriptomic insights and information for A. cristatum but also proposes a new method for exploring the functional genes of wheat relatives under a wheat genetic background. The sequence data have been deposited in the NCBI under BioProject accession number PRJNA534411.

scholarly journals TaGRF3-2A Improves Some Agronomically Valuable Traits in Semi-Dwarf Spring Triticale

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2012
Author(s):  
Mikhail Divashuk ◽  
Anastasiya Chernook ◽  
Aleksandra Kroupina ◽  
Milena Vukovic ◽  
Gennady Karlov ◽  
...  
Keyword(s):  
Growth Regulation ◽  
Stem Elongation ◽  
Grain Weight ◽  
Thousand Grain Weight ◽  
Marker Assisted Breeding ◽  
Negative Effects ◽  
Spring Triticale ◽  
Allelic State ◽  
Size Growth ◽  
Economically Valuable Traits

The breeding improvement of triticale is tightly associated with the introgression of dwarfing genes, in particular, gibberellin (GA)-insensitive Ddw1 from rye. Despite the increase in harvest index and resistance to lodging, this gene adversely affects grain weight and size. Growth regulation factor (GRF) genes are plant-specific transcription factors that play an important role in plant growth, including GA-induced stem elongation. This study presents the results of a two-year field experiment to assess the effect of alleles of the TaGRF3-2A gene in interaction with DDW1 on economically valuable traits of spring triticale plants grown in the Non-Chernozem zone. Our results show that, depending on the allelic state, the TaGRF3-2A gene in semi-dwarf spring triticale plants influences the thousand grain weight and the grain weight of the main spike in spring triticale, which makes it possible to use it to compensate for the negative effects of the dwarfing allele Ddw1. The identified allelic variants of the TaGRF3-2A gene can be included in marker-assisted breeding for triticale to improve traits.

Genetic Variation of Quantitative and Quality Traits in Rice Varieties

2020 ◽  
Vol 23 (1) ◽  
pp. 69-77
Author(s):  
MM Yasmin ◽  
NA Ivy ◽  
MG Rasul ◽  
MM Hossain
Keyword(s):  
Coefficient Of Variation ◽  
Significant Positive Correlation ◽  
Grain Weight ◽  
Genetic Advance ◽  
Thousand Grain Weight ◽  
Rice Varieties ◽  
Positive Correlation ◽  
High Heritability ◽  
Important Trait ◽  
Positive Direct

An investigation was carried out to study the genetic parameters and character association for yield, yield attributing, quality and nutritional traits of twelve rice varieties. Analysis of variance revealed significant differences for all the traits under study indicated the presence of wide genetic variability among the genotypes. Small differences between genotypic coefficient of variation and phenotypic coefficient of variation were recorded for all the characters studied which indicated less influence of environment on these characters. High heritability coupled with high genetic advance was observed for zinc content (brown) and number of chaffy grain per panicle exhibited, indicating that simple selection could be effective for improving these characters. Yield per hill had the highest positive direct effect. This study revealed that yield per hill, days to 50% flowering, days to maturity, panicle length and thousand grain weight are the most important yield contributing characters and an attempt should, therefore, be made for effective selection on these traits. Yield per hill might be considered as the most important trait due to low difference of GCV and PCV, high heritability in broad sense (h2b) with high genetic advance (GA) in percent of mean, significant positive correlation and high positive indirect effects. Another important trait such as days to 50% plants to flowering and thousand grain weight might also be considered due to their minimum difference of GCV and PCV values, high h2b with high GA in percent of mean, significant positive correlation and higher positive direct effects. Ann. Bangladesh Agric. (2019) 23(1) : 69-77

A comparison of cytoplasmic and chemically-induced male sterility systems for hybrid performance in wheat ( Triticum aestivum L.)

2006 ◽  
Vol 54 (1) ◽  
pp. 109-120 ◽  
Author(s):  
A. Adugna ◽  
A. Adugna ◽  
G. S. Nanda ◽  
G. S. Nanda ◽  
N. S. Bains ◽  
...  
Keyword(s):  
Grain Yield ◽  
Male Sterility ◽  
Triticum Aestivum L ◽  
Grain Weight ◽  
Hybrid Performance ◽  
Thousand Grain Weight ◽  
Yield Performance ◽  
Chemically Induced ◽  
B Lines ◽  
Better Than

Cytoplasmic ( Triticum timopheevii -based) and chemically induced (CH9832- based) male sterility systems were compared for hybrid performance in wheat. A total of 40 genotypes including 10 CMS-based hybrids, 10 CHA-based hybrids, 10 B lines and 10 R lines were included in the experiment. analysis of variance revealed significant differences between the genotypes for all the characters studied. Differences between the hybrids and their parents were significant for all the characters. There were also significant differences between the hybrids resulting from the two systems of sterility. This study of 10 comparable hybrids showed, on average, midparent heterosis of 30.2 and 7.3% for CMS- and CHA-derived hybrids, respectively. Generally, the CMS-based hybrids were superior to their CHA-based equivalents for grain yield performance. In spite of the incomplete fertility the higher grain yield in CMS-based hybrids was attributed to the profuse tillering and high thousand-grain weight. On the other hand, the CHA-based hybrids had lower yield performance due to the lower thousand-grain weight and tillering. The lower grain weight and tillering in these hybrids compared with their CMS-based equivalents might be due to the toxic effect of the CHA, which was carried over to the hybrid and affected vigour. As a whole the CMS system was found to be better than the CHA system (based on the particular CHA used in this study) for hybrid performance in wheat.

QTL analysis for thousand-grain weight under terminal drought stress in bread wheat (Triticum aestivum L.)

Euphytica ◽  
2011 ◽  
Vol 186 (1) ◽  
pp. 127-138 ◽  
Author(s):  
Khalil Zaynali Nezhad ◽  
W. E. Weber ◽  
M. S. Röder ◽  
S. Sharma ◽  
U. Lohwasser ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Drought Stress ◽  
Bread Wheat ◽  
Qtl Analysis ◽  
Triticum Aestivum L ◽  
Grain Weight ◽  
Thousand Grain Weight ◽  
Terminal Drought ◽  
Terminal Drought Stress

scholarly journals TaFlo2-A1, an ortholog of rice Flo2, is associated with thousand grain weight in bread wheat (Triticum aestivum L.)

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Muhammad Sajjad ◽  
Xiaoling Ma ◽  
Sultan Habibullah Khan ◽  
Muhammad Shoaib ◽  
Yanhong Song ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Triticum Aestivum L ◽  
Grain Weight ◽  
Thousand Grain Weight

scholarly journals Variability in yield traits of TILLING population of bread wheat (Triticum aestivum L.)

2015 ◽  
Vol 7 (1) ◽  
pp. 443-446
Author(s):  
Rajender Singh ◽  
Ratan Tiwari ◽  
Davinder Sharma ◽  
Vinod Tiwari ◽  
Indu Sharma
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Genetic Resource ◽  
Crop Improvement ◽  
Triticum Aestivum L ◽  
Grain Weight ◽  
Thousand Grain Weight ◽  
Tilling Population ◽  
Chemically Induced ◽  
Normal Seed

Mutagenesis is one of the powerful genetic strategies for crop improvement programmes. A chemically induced mutated genetic resource for detecting novel variations by Targeting Induced Local Lesions IN Genomes (TILLING) has been developed in recently released bread wheat (Triticum aestivum) cultivar DPW621-50. A total of 3,478 M2 plants were evaluated for plant height, number of tillers/plant, thousand grain weight, number of seeds/spike and grain yield/plant. A large variation was observed for all the traits. The highest frequency (52.2%) of lines had similar height between 91-100 cm to the non-mutagenized DPW 621-50 control followed by 28.9% of lines with height between 81-90 cm. A large variation was observed in number of tillers/plant which ranged from 1-35 tillers/plant. The highest frequency (32.09%) lines had 31-40 seeds/spike followed by 29.84% lines with 41-50 seeds/spike. Few lines (0.35%) had more than 70 seeds/spike with normal seed size as their thousand grain weight ranged between 34.82-43.82g. Chlorophyll deficient, grassy type and sterile mutants were also observed. This population may serve as new genetic resource for functional genomics studies and novel variants for different traits in elite germplasm can be made available to the plant breeders for wheat improvement.

Sign in / Sign up

Export Citation Format

Share Document