scholarly journals Comparative study on different field tests of ketosis using blood, milk, and urine in dairy cattle

2020 ◽  
Vol 65 (No. 5) ◽  
pp. 199-206
Author(s):  
Md. SA Faruk ◽  
B Park ◽  
S Ha ◽  
SS Lee ◽  
LL Mamuad ◽  
...  

This study assessed the diagnostic performance of five field tests for ketosis in the blood, milk and urine in dairy cows. Samples were collected simultaneously from 102 dairy cows to determine the accuracy of the test results. The concentration of β-hydroxybutyrate (BHBA) in the blood was measured quantitatively by using a portable ketone test kit. The milk and urine samples were analysed semi-quantitatively using different commercial test kits and urine sticks. The animals were categorised into a subclinical ketosis (SCK) and clinical ketosis (CK) group based on the BHBA concentration in the blood. The diagnostic performance of the milk and urine test kits was compared with the blood test kit. In the blood test, out of 102 cows, 27 and 19 cows were diagnosed with SCK and CK, respectively. The percentage agreement (kappa value) for the three different milk tests and the urine test kit was 43% (κ = −0.181), 72% (κ = 0.243), 63% (κ = 0.065) and 64% (κ = 0.163), respectively, for the SCK detection, which indicates lower sensitivity. However, a strong agreement with 88% (κ = 0.612), 85% (κ = 0.505), 86% (κ = 0.528) and 91% (κ = 0.753) was observed in the three milk tests and the urine test, respectively, for the CK diagnosis, showing a higher sensitivity. For the diagnosis of the SCK and CK, all the tested kits are generally applicable for the detection of CK in dairy cattle, but caution is needed during the interpretation of the SCK data due to the low sensitivity of the milk and urine tests for the SCK detection. Furthermore, this study suggests that milk and urine samples can be used as alternatives for the diagnosis of CK in replacement of blood tests.

2020 ◽  
Vol 72 (1) ◽  
pp. 1-8
Author(s):  
P.M. Soares Filho ◽  
A.K. Ramalho ◽  
A.M. Silva ◽  
M.A. Issa ◽  
P.M.P.C. Mota ◽  
...  

ABSTRACT Bovine tuberculosis is a worldwide spread zoonotic disease. Intradermal tuberculinizations are the most used diagnostic tests in the world. Serological tests can be an ancillary diagnosis for bovine tuberculosis. The objective of this study was to evaluate the diagnostic performance of the ELISA Mycobacterium Bovis Antibody Test Kit IDEXX ™ in infected herds, which were in different disease control stages. One hundred and twenty animals from two dairy herds of Minas Gerais state, Brazil, were subjected to the ELISA serological test and the comparative cervical tuberculin test (CCT). Diagnostic test parameters were estimated using Bayesian latent class models and concordance between tests estimated by the frequentist approach. The ELISA test presented lower sensitivity than CCT in both herds. Its sensitivity was higher in the herd in sanitation process. Specificity estimates were above 95% in both herds. Kappa index indicated low concordance or even disagreement between tests. According to the results, the ELISA IDEXX should not be used as substitution for CCT. The tests must not be associated in series. Parallel association increased diagnostic sensitivity in the herd which was in the process of sanitation.


2019 ◽  
Vol 5 (3) ◽  
pp. 388
Author(s):  
Amir Husaini Karim Amrullah ◽  
Dadang Suherman ◽  
Jarmuji Jarmuji

Giving sakura block plus that contain katuk leaf and turmeric flour are expected to increase the production of milk from dairy cow in Sumber Mulya livestock group. Community service activities are carried out through several stages: socialization, training, demonstration plots, and field tests. Socialization aims to provide an understanding of the purpose and objectives of the activity. The training has several objectives, namely the introduction of sakura block plus supplements, understanding the benefits of sakura block plus supplements for dairy cattle, materials and equipment used, and practices. Making sakura block plus begins with weighing ingredients, then mixed them until blended. Then, heat the brown sugar that has been mixed with 20% water. After that, mix the ingredients with brown sugar. Next, sakura block plus is pressed by presses tool. The last stage is wrapping it using plastic wrap. The demonstration plot and field test were conducted for three months and using three dairy cattle. The forage consumption of dairy cows used in demonstration plots ranges from 40-45 kg/head/day. Milk production in Sumber Mulya livestock group is 10.28 liters/head/day. The use of sakura blok can increase milk production 2 until 3 liters/head/day. The average milk production after using sakura block plus can reach 13 liters/head/day.Keywords: katuk leaf; livestock group; milk; suplement.


2000 ◽  
Vol 70 (3) ◽  
pp. 92-101 ◽  
Author(s):  
Burim Ametaj ◽  
Brian Nonnecke ◽  
Ronald Horst ◽  
Donald Beitz

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.


2016 ◽  
Vol 52 ◽  
pp. 6-12 ◽  
Author(s):  
M. V. Gladiy ◽  
G. S. Kovalenko ◽  
S. V. Priyma ◽  
G. A. Holyosa ◽  
A. V. Tuchyk ◽  
...  

The main goal of dairy breeds selection should be improving breeding and productive qualities of animals under modern conditions. The majority of farms, using native breeds to produce milk, has created optimal conditions for keeping and feeding, selection and matching, growing of replacements etc. Further improvement of created native dairy breeds for economically useful traits occurs at total use of purebred Holstein bulls (semen) of foreign selection. In order to realistically assess milk productivity (milk yield, fat content in milk and fat yield) of Ukrainian Black-and-White and Red-and-White Dairy cows should be conducted a comparative analysis of Holstein cows under the same conditions of feeding and keeping. It was established that Ukrainian Red-and-White Dairy cows were characterized by the highest milk yields for 305 days of all lactations, taken into account, the among three investigated breeds. Their milk yield during the first lactation was 5933 kg of milk, during the second – 6393 kg, the third – 6391 kg and during higher lactation – 6650 kg. Ukrainian Black-and-White Dairy cows were second by milk yield (except for the second lactation), during the first lactation – 5932 kg of milk, the third – 6462 kg and higher – 6541 kg, and Holstein cows were third, during the first lactation – 5794 kg of milk, the second – 6381 kg, the third – 6335 kg and higher – 6469 kg. The fat content was almost the same and varied within 3.49-3.58% in milk of Ukrainian Red-and-White Dairy cattle, 3.50-3.60% in milk of Ukrainian Black-and-White Dairy cattle and 3.50-3.56% in Holsteins’ milk. The difference between the breeds was within 0.01-0.04%. All the investigated breeds had predominance in fat yield for three lactations over standards of these breeds: Ukrainian Red-and-White Dairy cows from 75.1 to 93.4 kg, Ukrainian Black-and-White Dairy cows – 75.1-89.0 kg respectively and Holstein cows – 41.9-60.2 kg. It was found different level of positive correlation between milk yield and fat yield in all the cases and high correlation (r = 0.604-0.921, P < 0.001) in five cases (41.7%) Negative correlation coefficients indicate that selection of animals to higher milk yield in the herd will decrease the second trait – fat content in milk. Positive and highly significant correlation between milk yield and fat yield indicates that selection of cows in the herd to higher milk yields will increase fat yield. It was revealed that bulls were among the factors impacted the milk productivity (milk yield, fat content, fat yield) of three investigated breeds. So, the force (η²x) of father’s impact on milk yield was15.4-47.9%, fat content – 22.0-43.4% and fat yield – 14.9-47.7% taking into account a lactation and a breed. The force of lines impact (η²x) was second; it was on milk yield 6.1-24.5%, fat content – 4.1-17.1 and fat yield – 5.8-23.5%. The force of breeds impact (η²x) was last; it was on milk yield 0.3-2.9%, fat content – 0.2-0.3% and fat yield – 0.6-2.7%. So, the comparative studies of milk productivity of Ukrainian Red-and-White and Black-and-White Dairy cattle with Holsteins indicate that under similar conditions of feeding and keeping, these native breeds can compete with Holstein cattle. The milk yield for 305 days of higher lactation was 6650 kg of milk in Ukrainian Red-and-White Dairy cows, 6541 kg in Ukrainian Black-and-White Dairy cows and 6469 kg in Holsteins. It was found the inverse correlation r = -0.025-0.316 between milk yield and fat content in milk in most cases. Selection and matching of animals in the herd should be carried out simultaneously on these traits. It was found positive repeatability of milk yields between the first and second, the third and higher lactations (rs = 0.036-0.741), indicating the reliability of forecasting increase in milk productivity during the next lactations in all herd. Bulls have the greatest impact (η²x) on milk productivity among the factors taken into account: milk yield – 15.4-47.9%, fat content in milk – 22.0-43.4% and fat yield – 14.9-47.7%.


Author(s):  
Felix K. Shima ◽  
Fidelis A. Gberindyer ◽  
Matthew T. Tion ◽  
Olusegun A. Fagbohun ◽  
Temidayo O. Omobowale ◽  
...  

1953 ◽  
Vol 20 (1) ◽  
pp. 1-12 ◽  
Author(s):  
C. P. Cox

1. Some questions concerning the interpretation of observations on milking dairy cattle are considered.2. A formula is presented for analysing the contributions of changes in the numerator and denominator to the changes in a percentage or fraction, and the formula is applied to changes in the composition of milk.3. The depressions of the butterfat and solids-not-fat percentages consequent on diets reduced in hay in a dairy-cattle feeding experiment are examined with this constituent analysis.The contributions of changes in the magnitudes of the aqueous, butterfat and solids-not-fat constituents to the difference between the butterfat percentages of cows on two treatments, 18 lb. hay/day (control) and 6 lb. hay /day, are about equal. There is an apparent qualitative difference between the effects of the two non-control treatments, and a reduced yield of butterfat would alone largely account for the lower butterfat percentage of cows on the 2 lb. hay /day treatment.


2017 ◽  
Vol 26 (3) ◽  
pp. 292-298 ◽  
Author(s):  
Cesar Augusto Barbosa de Macedo ◽  
Madlaine Frigo Silveira Barbosa de Macedo ◽  
Ana Carolina Miura ◽  
Alessandra Taroda ◽  
Sergio Tosi Cardim ◽  
...  

Abstract The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.


1970 ◽  
Vol 6 ◽  
pp. 91-96
Author(s):  
M Saiful Islam ◽  
Susanta Kumar Kundu

Impact of genotypes and parity on some vital reproductive and productive attributes in the local (L×L, n = 100) and four crossbred cows (L×F, L×SL, L×JR and L×S; n = 318) raised in randomly selected smallholder dairy farms scattered all over Natore District and adjacent areas have been assessed during a period from September 2007 to June 2010. With regard to reproductive attributes, significant differences existed among the cattle genotypes (P<0.05) except for gestation length (GL) and age at weaning (AW). The lowest age at puberty (AP) was found for L×F (21.42±0.37 months), while the highest for L×L (31.67±0.74 months). In terms of productivity, L×F cows produced the highest daily milk yield (DMY; 6.22±0.13 L), coupled with the highest total lactation yield (TLY; 2163.43±47.77 L), while L×L produced the lowest values (1.49±0.04 L and 416.40±12.3 L, respectively) for the traits. The effect of parity on both reproductive and productive attributes showed that the middle-aged dairy cows of the 3rd and 4th parities performed better than the younger (1st and 2nd parities) or the older (5th and beyond) ones. Considering the overall performance, the L×F cows could be ranked as the best genotype followed by their L×SL, L×JR, L×S and L×L counterparts in the study area. DOI: http://dx.doi.org/10.3329/jles.v6i0.9727 JLES 2011 6: 91-96


1999 ◽  
Vol 37 (4) ◽  
pp. 1100-1106 ◽  
Author(s):  
Prudencio Martínez Martínez ◽  
Antonio Rodríguez Torres ◽  
Raul Ortiz de Lejarazu ◽  
Ana Montoya ◽  
José Francisco Martín ◽  
...  

The aim of the present study was to evaluate the possible utilization of saliva and urine as alternative samples to serum for the diagnosis of human immunodeficiency virus (HIV) infection. A total of 302 individuals participated in the study: 187 HIV-infected individuals (106 had Centers for Disease Control and Prevention [CDC] stage II infection, 19 had CDC stage III infection, and 62 had CDC stage IV infection) and 115 noninfected persons (46 of the noninfected persons were blood donors and 69 belonged to a group at high risk of HIV infection). Paired saliva and urine samples were taken from each of the participants in the study. The presence of HIV-specific antibodies was detected by an enzyme-linked fluorescent assay (ELFA), and the result was confirmed by Western blot analysis (WB). The ELFA with saliva gave maximum sensitivity and specificity values, while ELFA had lower sensitivity (95.2%) and specificity (97.4%) values for detection of HIV antibody in urine samples. WB with all saliva samples fulfilled the World Health Organization criterion for positivity, while only 96.8% of the urine samples were confirmed to be positive by WB. Among the four reactivity patterns found by WB of these alternative samples, the most frequent included bands against three groups of HIV structural proteins (was ENV, POL, and GAG). The reactivity bands most frequently observed were those for the proteins gp160 and gp120. The least common reactivity band was the band for protein p17. The detection of HIV antibodies in saliva samples by means of ELFA with the possibility of later confirmation by WB makes saliva an alternative to serum for possible use in the diagnosis of infection. In contrast, HIV antibody detection in urine samples by the same methodology (ELFA) could be taken into consideration for use in epidemiological studies.


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