scholarly journals Identification and Analysis of Plant Growth Promoting Bacteria in LEAF Community Garden Soil

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Pooja Ramadas ◽  
Dhruv Pathak ◽  
Prabhjeet Kaur
Keyword(s):  
Plant Growth ◽  
Crop Yield ◽  
Dna Sequences ◽  
Bacterial Species ◽  
Garden Soil ◽  
Community Garden ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
Growth Promoting

In today’s largely populated modern world, crop yield is becoming increasingly important. To increase crop yield, new modern technologies for farming are continuously being innovated. The aim of this study is the identification of Plant Growth Promoting Bacteria (PGPBs) and their properties. In order to conduct the experiment, soil samples were collected from the community garden LEAF (Local Ecology and Agriculture Fremont). These samples were grown in Luria Bertani agar plates, and the two bacterial strains that grew from them were analyzed to determine the species of the bacteria. Using a DNA extraction kit, DNA was extracted from the bacteria and then amplified versions were sent to RF Biotech for DNA sequencing. The DNA sequences were then used to determine that the two bacterial species in question are Bacillus cereus and Morganella morganii. Afterwards, multiple assays were used to measure the efficiency of each bacterial species to absorb various substances that would be helpful for plant growth. The aim of this research is to better understand which bacterial strains are beneficial for plants, and which are harmful. Through having greater zones of inhibition, the bacterial species M. morganii proved to be more efficient in the siderophore and phosphate solubilization assays. In contrast, the bacterial species B. cereus proved to be more efficient in the cellulase and amylase production assays. These results will assist LEAF in enriching their soil in order to increase their crop yields by creating an increase in concentration of advantageous bacteria and decrease that of detrimental bacteria.

scholarly journals Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 912
Author(s):  
Shuming Liu ◽  
Hongmei Liu ◽  
Rui Chen ◽  
Yong Ma ◽  
Bo Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Translocation Factor ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Cd Tolerance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Cd Translocation

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

scholarly journals The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 888
Author(s):  
Giorgia Novello ◽  
Patrizia Cesaro ◽  
Elisa Bona ◽  
Nadia Massa ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Global Market ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Mineral Concentration ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Onion Cultivar ◽  
Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

scholarly journals Growth response of wheat cultivars to bacterial inoculation in calcareous soil

2010 ◽  
Vol 56 (No. 12) ◽  
pp. 570-573 ◽  
Author(s):  
D. Egamberdieva
Keyword(s):  
Plant Growth ◽  
Calcareous Soil ◽  
Dry Weight ◽  
Plant Growth Promoting Bacteria ◽  
Wheat Cultivars ◽  
Bacterial Strains ◽  
Bacterial Inoculation ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Selection Of

In this study the plant growth-promoting bacteria were analysed for their growth-stimulating effects on two wheat cultivars. The investigations were carried out in pot experiments using calcareous soil. The results showed that bacterial strains Pseudomonas spp. NUU1 and P. fluorescens NUU2 were able to colonize the rhizosphere of both wheat cultivars. Their plant growth-stimulating abilities were affected by wheat cultivars. The bacterial strains Pseudomonas sp. NUU1 and P. fluorescens NUU2 significantly stimulated the shoot and root length and dry weight of wheat cv. Turon, whereas cv. Residence was less affected by bacterial inoculation. The results of our study suggest that inoculation of wheat with Pseudomonas strains can improve plant growth in calcareous soil and it depends upon wheat cultivars. Prior to a selection of good bacterial inoculants, it is recommended to select cultivars that benefit from association with these bacteria.

scholarly journals Micro-remediation of chromium contaminated soils

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6076 ◽  
Author(s):  
Hadia -e- Fatima ◽  
Ambreen Ahmed
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Crop Production ◽  
Lens Culinaris ◽  
Production Plant ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Soil Release ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacteria are tiny organisms which are ubiquitously found in the environment. These microscopic living bodies are responsible for the flow of nutrients in biogeochemical cycles and fertility imparted to the soil. Release of excessive chromium in agricultural soils due to rapid growth of industries may result in minimizing the fertility of soil in future, which will lead to reduction in crop production. Plant growth promoting bacteria (PGPB) are beneficial to the environment, some of which can tolerate chromium and protect plants against heavy metal stress. The current study aims to identify such chromium-tolerant auxin-producing rhizobacteria and to investigate their inoculation effects on the growth characteristics of Lens culinaris in chromium polluted soils by using two different chromium salts i.e., K2Cr2O7 and K2CrO4 in varying concentrations (0, 50, 100, 200, 400 and 500 µgml−1). The results revealed that Bacillus species are efficient in significantly reducing the deleterious effects of Cr. These effective bacterial strains were able to stimulate the growth of metal effected plants of Lens culinaris which were grown in chromium contaminated environment. Therefore, these plant growth promoting rhizobacteria PGPRs, having both auxin production potential and chromium-resistance ability, are considered as efficient micro-factories against chromium pollution.

scholarly journals Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

2021 ◽  
Vol 8 (sp1) ◽  
pp. 17-24
Author(s):  
Tanvir Kaur ◽  
Rubee Devi ◽  
Divjot Kour ◽  
Ashok Yadav ◽  
Ajar Nath Yadav
Keyword(s):  
Plant Growth ◽  
Environmental Sustainability ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Sugar Content ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

Associations of bacterial endophyte populations from red clover and potato crops with potential for beneficial allelopathy

1998 ◽  
Vol 44 (2) ◽  
pp. 162-167 ◽  
Author(s):  
A V Sturz ◽  
B R Christie ◽  
B G Matheson
Keyword(s):  
Plant Growth ◽  
Crop Rotation ◽  
Bacterial Species ◽  
Root Weight ◽  
Plant Growth Promoting Bacteria ◽  
Shoot Height ◽  
Plant Growth Promoting ◽  
Wet Weight ◽  
Growth Promoting ◽  
Growth Inhibiting

Clover and potatoes, in a crop rotation, were found to share specific associations of bacterial endophytes. Twenty-five bacterial species from 18 genera were common to both clover and potatoes and represented 73% of all the bacteria recovered from clover root tissues and 73% of all the bacteria recovered from potato tubers. Endophytic bacteria tested in potato plant bioassays were predominantly plant growth neutral (56%). The remainder were either plant growth promoting (21%) or plant growth inhibiting (24%)(P < 0.05). Of the plant growth promoting bacteria, 63% increased shoot height, 66% increased shoot wet weight, and 55% increased root wet weight. The effects of plant growth inhibiting bacteria were restricted to reductions in plant height (86%) and shoot wet weight (36%); root weight was not affected. Of the bacteria tested, 74% showed some degree of in vitro antibiosis to the clover and potato pathogen Rhizoctonia solani. Such endophytic intercrop bacterial associations appear to be complementary in nature and support the view that there are microbial benefits to be gained from clover in crop sequences with potatoes, beyond those of the residual nitrogen left in the soil and the organic matter added.Key words: Trifolium, allelopathy, endophytic, plant growth promoting, antifungal, crop rotation, Solanum.

scholarly journals Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

2015 ◽  
Vol 81 (19) ◽  
pp. 6700-6709 ◽  
Author(s):  
Maria Isabel Stets ◽  
Sylvia Maria Campbell Alqueres ◽  
Emanuel Maltempi Souza ◽  
Fábio de Oliveira Pedrosa ◽  
Michael Schmid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Quantitative Pcr ◽  
Dna Sequences ◽  
Azospirillum Brasilense ◽  
Plant Growth Promoting Bacteria ◽  
Genome Sequences ◽  
Wheat Roots ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACTAzospirillumis a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide.Azospirillum brasilensestrains exhibit a growth-promoting effect by means of phytohormone production and possibly by N2fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of the inoculant in the rhizosphere, which is not always achieved. The objective of this study was to develop quantitative PCR protocols for the strain-specific quantification ofA. brasilenseFP2. A novel approach was applied to identify strain-specific DNA sequences based on a comparison of the genomic sequences within the same species. The draft genome sequences ofA. brasilenseFP2 and Sp245 were aligned, and FP2-specific regions were filtered and checked for other possible matches in public databases. Strain-specific regions were then selected to design and evaluate strain-specific primer pairs. The primer pairs AzoR2.1, AzoR2.2, AzoR5.1, AzoR5.2, and AzoR5.3 were specific for theA. brasilenseFP2 strain. These primer pairs were used to monitor quantitatively the population ofA. brasilensein wheat roots under sterile and nonsterile growth conditions. In addition, coinoculations with other plant growth-promoting bacteria in wheat were performed under nonsterile conditions. The results showed thatA. brasilenseFP2 inoculated into wheat roots is highly competitive and achieves high cell numbers (∼107CFU/g [fresh weight] of root) in the rhizosphere even under nonsterile conditions and when coinoculated with other rhizobacteria, maintaining the population at rather stable levels for at least up to 13 days after inoculation. The strategy used here can be applied to other organisms whose genome sequences are available.

scholarly journals Plant growth-promoting bacteria belonging to the genera Pseudomonas and Bacillus improve the growth of sorghum seedings in a low-nutrient soil

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Enriqueta Amora-Lazcano ◽  
Héctor J. Quiroz-González ◽  
Cristofer I. Osornio-Ortega ◽  
Juan A. Cruz-Maya ◽  
Janet Jan-Roblero
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Dry Weight ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Shoot Dry Weight ◽  
Soil Bioassay ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background: Deficiency in sorghum growth in ecosystems of low-nutrient soils has been scarcely studied. This soil deficiency can be overcome by the addition of plant growth-promoting bacteria which increase sorghum growth. Questions and/or Hypotheses: indole acetic acid (IAA) producing and phosphate solubilizing bacteria can promote sorghum growth under nutritional stress. Studied species: Sorghum bicolor (L.) Moench. Study site and dates: Mexico City, 2018. Methods: Of the twelve bacterial strains utilized, three produce IAA (group BI), two strains produce IAA and siderophores (BIS group), four strains produce IAA and solubilize phosphate (BIP group), and three strains produce IAA, solubilize phosphate, and produce siderophores (BIPS group). Hydroponic bioassays and low-nutrient soil bioassay were used. Results: In hydroponic bioassays, for BI and BIS groups, five strains significantly increased the growth parameters with respect to the control, and for the BIP and BIPS groups, two strains promoted stem development and shoot dry weight. In a low-nutrient soil bioassay, Pseudomonas sp. BI-1 (from BI group) was the one that presented the highest percentages 32, 48, 140 and 79 % in stem diameter, height and dry weight of the shoot and dry weight of the root, respectively, followed by the P. mohnii BIPS-10 strain (from BIPS group) that exhibited similar results. Conclusions: IAA producing Pseudomonas strains improve the sorghum growth in a low-nutrient soil and suggest thatPseudomonas sp. BI-1 and P. mohnii BIPS-10 could be used as potential bioinoculants for sorghum.

scholarly journals Lysinibacillus acetophenoni and Pseudomonas stutzeri with High Salt effect, Recovered from High Salinity Soil Area (Indo-Gangetic Plain of India)

2021 ◽  
pp. 17-26
Author(s):  
Parul Bhatt Kotiyal ◽  
Soni Singh ◽  
Sunita Rawat ◽  
Vikesh Vyas ◽  
Himani Negi
Keyword(s):  
Plant Growth ◽  
Saline Soil ◽  
Halophilic Bacteria ◽  
Economic Value ◽  
Soil Microbial Communities ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Gangetic Plain ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil salinity has affected many soil microbial communities as well as economic value of forest ecosystem for many years. The plant growth-promoting bacteria have developed several different mechanisms that have a positive influence on plant development and growth. Designated strain L-PB424 and P-PB466 was isolated and identified from saline soil of block Ashabutter khair forest in Punjab North zone in India, were investigated for their plant growth-promoting characters such as production of indole acetic acid, phosphate solubilization, Ammonium production and fermentation of polysaccharides. Comparative analysis of 16SrRNA gene sequences revealed that L-PB424 was closely related to Lysinibacillus manganicus DSM 26584 strain Mn1-7 (98.76%), on the other hand strain P-PB466 was closely related to Pseudomonas songnenensis strain NEAUST5-5. This research paper is a study in evaluation and variety of possible halophlic/halotolerant bacterial strains in salt-affected soils of block Ashabutter khair forest in Punjab North zone in India. The use of Halophilic bacteria in saline soil is interesting for future analysis and biotechnological development.

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