Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast

2014 ◽  
Vol 14 (2) ◽  
pp. 107
Author(s):  
Ji Uk Yoon ◽  
Sang Wook Shin ◽  
Bong Soo Park ◽  
Yong Ho Kim ◽  
Mi Na Woo ◽  
...  
Keyword(s):  
Human Osteoblast

Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

2020 ◽  
Vol 22 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Lin-Jun Sun ◽  
Chong Li ◽  
Xiang-hao Wen ◽  
Lu Guo ◽  
Zi-Fen Guo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Chinese Herb ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Expression Ratio ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

scholarly journals The Nitrogen-Containing Bisphosphonate, Zoledronic Acid, Influences RANKL Expression in Human Osteoblast-Like Cells by Activating TNF-α Converting Enzyme (TACE)

2004 ◽  
Vol 19 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Beiqing Pan ◽  
Amanda N Farrugia ◽  
Luen Bik To ◽  
David M Findlay ◽  
Jonathan Green ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Human Osteoblast ◽  
Converting Enzyme ◽  
Rankl Expression ◽  
Tnf Α

Osteoinductive effect of soluble transforming growth factor beta receptor 3 on human osteoblast lineage

2021 ◽  
Author(s):  
Angelica Mastandrea Amanso ◽  
Archana Kamalakar ◽  
Sara Bitarafan ◽  
Shelly Abramowicz ◽  
Hicham Drissi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Human Osteoblast ◽  
Beta Receptor ◽  
Growth Factor Beta ◽  
Osteoblast Lineage

16. Transfection of human osteoblast-like cells with SV-40 DNA

Bone ◽  
1991 ◽  
Vol 12 (4) ◽  
pp. 290-290
Author(s):  
M.M. Williams ◽  
D.B. Evans ◽  
P.D. Delmas ◽  
M. Gowen
Keyword(s):  
Human Osteoblast

scholarly journals Overexpression of CRABP2 inhibits dexamethasone-induced apoptosis in human osteoblast cells

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Haiping Zhang ◽  
Ziliang Yu ◽  
Farui Sun ◽  
Jin Jin
Keyword(s):  
Protein Expression ◽  
Underlying Mechanism ◽  
R Package ◽  
Treated Group ◽  
Gene Expression Omnibus ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Gene And Protein Expression ◽  
Osteoblast Apoptosis ◽  
Induced Apoptosis

Abstract Background The purpose of the current study was to explore the role and underlying mechanism of cellular retinoic acid binding protein 2 (CRABP2) in dexamethasone (DEX)-induced apoptosis in human osteoblast cells. Methods GSE10311 was downloaded from the Gene Expression Omnibus (GEO) database to identify the differentially expressed genes (DEGs) by the limma/R package. Primary human osteoblast was isolated and treated with different concentration of DEX (0, 10-8, 10-7, 10-6, 10-5, and 10-4 mol/L), and cell viability and flow cytometry were used to detect cell proliferation and apoptosis. A CRABP2 overexpression plasmid (oe-CRABP2) was used to overexpress CRABP2, and western blotting was conducted to detect protein expression. Results We found that CRABP2 was downregulated in the DEX-treated group. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that DEGs were associated with PI3K/Akt signaling pathway. DEX downregulated CRABP2 gene and protein expression, inhibited viability, and induced human osteoblast apoptosis. Overexpression of CRABP2 reversed DEX-induced apoptosis in human osteoblast. Moreover, overexpression of CRABP2 delayed the progression of DEX-induced osteonecrosis of the femoral head (ONFH) animal model. Conclusion In conclusion, CRABP2 is effective at inhibiting DEX-induced human osteoblast apoptosis and delayed ONFH progression.

Detection and Characterization of Endothelin in Transformed Human Osteoblast Cell Culture Medium

Endocrine ◽  
2000 ◽  
Vol 12 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Hing-Chung Lam ◽  
Jenn-Kuen Lee ◽  
Kwok-Hung Lai
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Cell Culture Medium ◽  
Human Osteoblast Cell

Lecithin blended polyamide-6 high aspect ratio nanofiber scaffolds via electrospinning for human osteoblast cell culture

2011 ◽  
Vol 31 (2) ◽  
pp. 486-493 ◽  
Author(s):  
R. Nirmala ◽  
Hye-Min Park ◽  
R. Navamathavan ◽  
Hyung-Sub Kang ◽  
Mohamed H. El-Newehy ◽  
...  
Keyword(s):  
Cell Culture ◽  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Polyamide 6 ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Nanofiber Scaffolds
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