scholarly journals Obesity as a factor in spermatogenesis disorders (experimental study)

2020 ◽  
Vol 21 (2) ◽  
pp. 36-43
Author(s):  
A. A. Artamonov ◽  
S. V. Bogolyubov ◽  
T. I. Eliseeva ◽  
O. B. Pozdnyakov ◽  
A. V. Astakhova
Keyword(s):  
Total Cholesterol ◽  
Dna Fragmentation ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Study Objective ◽  
Diet Induced Obesity ◽  
White Rats ◽  
Sperm Dna ◽  
Significant Difference

Introduction. In recent years, the effects of obesity on male fertility have been extensively investigated. The results of existing studies are extremely contradictory.The study objective was to determine the effect of obesity on the male reproductive system using the biological model of laboratory rats as an example.Materials and methods. In vivo modeling of diet-induced obesity. The study was conducted on 22 laboratory sexually mature white rats weighing 140–160 g. The animals were divided into two groups: 1 control (10 animals) and 2 rats with diet-induced obesity (12 animals). After 12 weeks, the animals were removed from the experiment. All rats underwent: calculation of the Lee index (body mass index in rats), determination of the concentration and viability of spermatozoa in a suspension of sperm from the epididymis, determination of glucose level of total cholesterol and triglycerides in the blood, study of sperm DNA fragmentation, histological examination testis: calculating the crosssectional area of the seminiferous tubule; determination of the number of non-functioning tubules and tubules with desquamated spermiogenic epithelium; determination of the average spermatogenesis index.Results. In the study groups there were no differences in glucose and total cholesterol levels. However, a statistically significant, significant difference in the level of triglycerides in the blood was revealed. The concentration of sperm and their viability in the studied groups did not differ. The level of sperm DNA fragmentation in the experimental group is significantly higher than in the control group (31.5 ± 10.1 and ± 1.4 %, respectively, p <0.05). Morphometric evaluation of histological preparations did not establish differences in the cross-sectional area of the seminiferous tubules and the average spermatogenesis index in the studied groups. In rats with obesity, compared with the control group, significantly more non-functioning tubules (2.9 ± 0.3 and 8.4 ± 0.3; p <0.05) and tubules with desquamated spermatogenic epithelium (1.8 ± 0.3 and 8.8 ± 0.5; p <0.05).Conclusion. Diet-induced obesity causes impaired spermatogenesis, and damage to the sperm genetic material in male white rats.

scholarly journals FEATURES OF STRUCTURE AND DYNAMICS IN DAMAGES OF FERTILE PROPERTIES IN EJACULATE UNDER THE INFLUENCE OF TOXOCARIASIS INVASION

2020 ◽  
Vol 47 (4) ◽  
Author(s):  
L. L. Voroncova ◽  
S. O. Kenijz ◽  
V. A. Kovalenko
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Normal Level ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Treatment Regimens ◽  
Male Gametes ◽  
Sperm Dna ◽  
Infertility Patients

Abstract Purpose of the study. To study the features of changes of spermiologic values and degree of spermatozoa DNA fragmentation depending on the presence/absence of toxocariasis invasion. Materials and methods. 89 men aged 20 to 45 years were examined, which were divided into 5 groups. The first (control) group was 12 fertile men; the second group (comparison group) – 27 infertility patients with normal level of DNA fragmentation of sperm and without antibodies to toxocariasis; The third group – 20 infertility men with normal level of DNA fragmentation of sperm and presence of antibodies to toxocariasis. By the fourth and fifth groups included 15 infertility men with high levels of DNA fragmentation of sperm and the presence of antibodies to toxocariasis and lack of respectively. A comprehensive research was conducted for all men that included analysis of spermogram according to WHO recommendations, determination of sperm DNA fragmentation and presence of toxocariasis invasion. Results. As a result of spermograms in the studied groups of men were identified the violations of ejaculate fertility (asteno-, teratoand oligozoospermia). It has been established that the degree disturbance of spermatogenesis (including significant oligo- and teratozoospermia) is depending on the fragmentation of DNA in male gametes and the presence of antibodies to toxocara. At the same time more severe patozoospermia observed in the presence of elevated levels of fragmented sperms and the absence of antibodies to toxocara antigens, which is the basis for further research. Conclusions. Thus, the determination of sperm DNA fragmentation and availability of toxocariasis invasion in men with reproductive disorders is a necessary component of a comprehensive examination, which may allow to improve the diagnostics of male infertility and to promote the development of the right tactics and optimal treatment regimens. Keywords: male infertility, spermogram, sperm DNA fragmentation, toxocariasis invasion

DETERMINATION OF THE HUMAN SPERM DNA FRAGMENTATION INDEX

Vestnik ◽  
2021 ◽  
pp. 226-232
Author(s):  
К. К. Тлеуханов ◽  
Н. А. Алтыбаева ◽  
М. К. Отарбаев ◽  
Е. М. Тойшибеков ◽  
А. А. Тлеуханова
Keyword(s):  
Dna Fragmentation ◽  
Human Sperm ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Sperm Dna Fragmentation Index ◽  
Dna Fragmentation Index

В статье представлены собранные данные о методах устранения повышенной частоты фрагментации ДНК у сперматозоидов, которые в некоторых исследованиях подтверждают, что введение антиоксидантов может снизить уровень фрагментации ДНК у сперматозоидов. The article presents collected data on methods of eliminating the increased frequency of DNA fragmentation in spermatozoa, which in some studies confirm that the introduction of antioxidants can reduce the level of DNA fragmentation in spermatozoa.

P–068 CatSper4 cation channel expression is low in male infertility and is affected by cryopreservation

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
N Kilic ◽  
T İrez ◽  
N Dayiolu
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Middle Part ◽  
Cation Channel ◽  
The Other ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Negative Effects ◽  
Sperm Dna

Abstract Study question Is CatSper4 expression in sperm related to functional parameters and does cryopreservation affect CatSper4 expression? Summary answer In this study, it was aimed to investigate whether CatSper4 has a relationship with sperm parameters and is CatSper 4 affected by cryopreservation. What is known already CatSper membrane channels, known as cation channels, are thought to play an important role in the insufficiency of sperm physiology, acrosome reaction, and chemotaxis movement. There is no study on cation channel distribution in an infertile male patient. In addition, studies conducted in recent years have shown that cryopreservation techniques have negative effects on sperm DNA, but there is no analysis in the literature regarding the effects of cryopreservation on CatSper4 ion channel proteins. Study design, size, duration Samples of the patients who applied to the Andrology laboratory in the Medical Park Hospital IVF unit between March 1 and June 1 in 2020 were included in the study. Also, patients with no family history of no genetic anomalies , no varicocele and azoospermia were included.The study were divided into 4 groups in accordance with the male infertility guideline of the European Association of Urology as normozoospermic (control group), the asthenoteratozoospermia, teratozoospermia, and oligoastenotheratozoospermia. Participants/materials, setting, methods In this prospective study, semen analysis, DNA fragmentation, and CatSper 4 by IHC of control group patients with normospermia (n = 40) and oligospermia(n = 50), asthenospermia(n = 40), and teratozoospermia(n = 38) patients were compared and differences resulting from cryopreservation were evaluated by Wilcoxon signed Ranks Test. Main results and the role of chance It was observed that CatSper4 protein positivity was localized in the middle part of the sperm and it was statistically higher in the normozoospermic patient group compared to the other groups (p = 0,01). When the positivity values of CatSper4 protein before and after freezing were compared in the groups, it was seen that the values decreased (p = 0,001,p=0,01). Sperm DNA fragmentation was found to be lowest in normospermia and statistically significantly higher in other groups. Cryopreservation application increased DNA fragmentation in all groups (p &lt; 0,001 , p &lt; 0,01). Limitations, reasons for caution Unfortunately, embryo screening in patients with low CatSper4 expression is not available in the present study. Soon we plan to screen a broader clinical pregnancy series and present the IVF results associated with CatSper4. Wider implications of the findings: Our study indicated that, CatSper4 expression is quite high in normospermia when compared with the other groups, particularly oligoasthenoteratozoospermia and asthenoteratozoospermia. There are almost no studies on this subject in the literature, and we think that it should be studied in larger patient groups and in unexplained infertile cases. Trial registration number Not applicable

scholarly journals Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation

2020 ◽  
Vol 9 (12) ◽  
pp. 3976
Author(s):  
Alberto Pacheco ◽  
Arancha Blanco ◽  
Fernando Bronet ◽  
María Cruz ◽  
Jaime García-Fernández ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Cell Sorting ◽  
Oocyte Donation ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Sperm Selection ◽  
Reproductive Outcomes ◽  
Selection Technique ◽  
Sperm Dna ◽  
Magnetic Activated Cell Sorting

Magnetic-activated cell sorting (MACS) can be used to separate apoptotic sperm with high proportions of fragmented DNA from the rest, thus improving the overall quality of the seminal sample. Therefore, the aim of this retrospective study was to investigate the efficiency of the MACS technique to increase reproductive outcomes in patients with high levels of sperm DNA fragmentation (SDF) undergoing intracytoplasmic sperm-injection (ICSI) cycles. In this study, we analyzed a total of 724 assisted-reproduction-technique (ART) cycles that were divided into two groups: the study group (n = 366) in which the MACS selection technique was performed after density-gradient centrifugation (DGC), and the control group (n = 358) in which only DGC was used for sperm selection. Reproductive outcomes were analyzed in both groups according to three different ART procedures: preimplantation genetic testing for aneuploidy (PGT-A), and autologous and oocyte-donation cycles. The MACS group showed significantly lower miscarriage rates in autologous ICSI cycles, higher pregnancy rates in oocyte-donation cycles, and a significant increase in live-birth rates in both autologous and oocyte-donation cycles. Overall, these results suggested that the MACS technique can be effectively used to eliminate sperm with high SDF levels, and therefore may help to improve reproductive outcomes in couples undergoing ART.

scholarly journals Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Reproduction ◽  
2016 ◽  
Vol 151 (4) ◽  
pp. 379-390 ◽  
Author(s):  
Thais Rose dos Santos Hamilton ◽  
Letícia Signori de Castro ◽  
Juliana de Carvalho Delgado ◽  
Patrícia Monken de Assis ◽  
Adriano Felipe Perez Siqueira ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Ovis Aries ◽  
Sperm Dna Fragmentation ◽  
Mitochondrial Potential ◽  
Sperm Analysis ◽  
Sperm Dna ◽  
Significant Difference ◽  
Ram Sperm

Action of reactive oxygen species, protamination failures and apoptosis are considered the most important etiologies of sperm DNA fragmentation. This study evaluated the effects of induced lipid peroxidation susceptibility on native semen profile and identified the mechanisms involved in sperm DNA fragmentation and testicular antioxidant defense on Santa Ines ram sperm samples. Semen was collected from 12 adult rams (Ovis aries) performed weekly over a 9-week period. Sperm analysis (motility, mass motility, abnormalities, membrane and acrosome status, mitochondrial potential, DNA fragmentation, lipid peroxidation and intracellular free radicals production); protamine deficiency; PRM1, TNP1 and TNP2 gene expression; and determination of glutathione peroxidase (GPx), glutathione reductase, catalase (CAT) and superoxide dismutase activity and immunodetection in seminal plasma were performed. Samples were distributed into four groups according to the sperm susceptibility to lipid peroxidation after induction with ascorbate and ferrous sulfate (low, medium, high and very high). The results were analyzed by GLM test and post hoc least significant difference. We observed an increase in native GPx activity and CAT immunodetection in groups with high susceptibility to induced lipid peroxidation. We also found an increase in total sperm defects, acrosome and membrane damages in the group with the highest susceptibility to induced lipid peroxidation. Additionally, the low mitochondrial membrane potential, susceptible to chromatin fragmentation and the PRM1 mRNA were increased in the group showing higher susceptibility to lipid peroxidation. Ram sperm susceptibility to lipid peroxidation may compromise sperm quality and interfere with the oxidative homeostasis by oxidative stress, which may be the main cause of chromatin damage in ram sperm.

P–091 Magnetic Activated Cell Sorting (MACS) improves euploid blastocysts rate in pre-implantation genetic testing cycles with high levels of sperm DNA fragmentation and advanced paternal age

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
F Scarselli ◽  
E Cursio ◽  
A Colasante ◽  
V Zazzaro ◽  
P Andrea ◽  
...  
Keyword(s):  
Genetic Testing ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Annexin V ◽  
Paternal Age ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Number Of Patients ◽  
Blastocyst Rate

Abstract Study question Can MACS increase euploid blastocyst rate in Pre-implantation Genetic Testing (PGT) cycles for AMA-APA (Advanced Maternal-Paternal Age) in patients with high sperm DNA fragmentation (SDF)? Summary answer A slight increase in euploid blastocyst rate was found using MACS in infertile patients with high SDF undergoing PGT cycles compared to the control group. What is known already Many authors have shown a close correlation between the presence of apoptotic markers on spermatozoa and the failure of assisted reproduction treatments. In normal physiological conditions, apoptotic spermatozoa with phosphatidylserine (PS) residues externalized on the plasma membrane, are eliminated along female genital tract, preventing oocyte fertilization. MACS eliminates apoptotic sperm whit PS residues using superparamagnetic microbeads conjugated with annexin V. This technique reduces the proportion of sperm with high rates of SDF and can be used to maximize ART procedures results. MACS application improves sperm quality, fertilization, cleavage and pregnancy rates reducing miscarriage rate. Study design, size, duration From June to November 2020, 10 couples in which MACS was applied to select non-apoptotic spermatozoa, were randomly enrolled in our study (MACS group) and 8 couples without MACS were considered as controls (No-MACS Group). All couples in both groups underwent a PGT cycle and had high sperm DNA Fragmentation (&gt; 20%). A higher rate of euploid and diploid-euploid mosaic blastocysts were obtained in the MACS group compared to the control group. Participants/materials, setting, methods Patients with severe oligoastenoteratozoospermia were excluded. MACS protocol was performed as follows: semen sample was analyzed (WHO 2010) and washed with buffered medium; pellet was removed and a swim-up was performed. Retrieved spermatozoa were washed with a binding buffer (Miltenyi Biotec), centrifuged (400 g x 4 minutes) and supernatant discarded. Pellet was covered with Annexin-V and re-suspended. After 15 minutes incubation at room temperature, the sample was eluted through the column and collected for ICSI. Main results and the role of chance In MACS group, female and male mean age ± SD were 41.6 ± 2.1 and 43.5 ± 7.3, respectively. Female and male mean age ± SD were 41.7 ± 2.8 and 44.6 ± 8.1 in the No-MACS group, respectively. In MACS and No-MACS groups, injected oocytes were 44 and 35, fertilized oocytes were 32 (72.3%) and 27 (77.1%) (NS), blastocyst formation rates were 71.8% (23/32) and 48.1% (13/27) (NS), respectively. In No-MACS group, only 1 euploid and 1 diploid-euploid mosaic blastocysts were obtained (1/13 = 8%) (NS). In MACS group, 4 euploid blastocysts were formed (4/23 = 17.4%) whereas mosaic diploid-euploid blastocysts were 3/23 (13.0%) (NS). Aneuploid blastocysts were 16/23 (69.6%) in MACS group and 11/13 (84.6%) in No-MACS group (NS). Limitations, reasons for caution AMA and APA of couples enrolled should be considered as a limit of the study. A larger number of patients and biopsied blastocysts are needed to analyze clinical results and perform a robust statistical analysis establishing if MACS is useful to improve transferable blastocyst rate in patients with high SDF. Wider implications of the findings: MACS is useful to select non apoptotic sperms; although fertilization, cleavage and blastocyst rates are not improved, aneuploid blastocysts rate slightly decreases using MACS. It I possible that, selecting spermatozoa free from PS residues, MACS allows to choose spermatozoa with a better DNA packaging, thus affecting the embryo ploidy. Trial registration number non applicable

The Association Between Sperm DNA Fragmentation and Idiopathic Early Recurrent Pregnancy Loss

KnE Medicine ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Binarwan Halim
Keyword(s):  
Dna Fragmentation ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Control Group ◽  
Case Group ◽  
Sperm Dna Fragmentation ◽  
Male Factor ◽  
Sperm Dna ◽  
History Of ◽  
Group 5

<p><strong>Introduction</strong></p><p>Various etiologies of recurrent pregnancy loss (RPL) have been extensively studied, but more than half of them still remain unknown. Male factor may play a role in incidence of idiopathic early recurrent pregnancy loss. Sperm DNA fragmentation as one of sperm test for male factor can be measured and expressed by a DNA Fragmentation Index (DFI). The aim of the study is to evaluate the association between sperm DNA fragmentation and the incidence of idiopathic early recurrent pregnancy loss.</p><p><strong>Material &amp; Methods</strong></p><p>A prospective study was done by recruiting 40 cases of  male couple from patients with a history of idiopathic early recurrent pregnancy loss and 40 cases of control from normal fertile population from May 2010 to September 2011 in Halim Fertility Center. Sperm DNA fragmentation was detected by halosperm kit.</p><p><strong>Results</strong><br />Both of groups were comparable in terms of the age of male patients, body mass index, duration of infertility, history of miscarriage and sperm parameters. Sperm DFI in the case group was 34.12%. and in the control group was 16.02%. There was significantly higher sperm DFI in the case group than in the control group. Sperm DFI &lt;30 was increased in control group (95%) compared with case group (40%). Sperm DFI ≥30 was increased in case group (60%) compared with control group (5%). There was a significant association between sperm DFI ≥30 and idiopathic early recurrent pregnancy loss (p&lt;0,05).</p><p><strong>Conclusion</strong></p><p>There is an association between higher sperm DNA fragmentation and incidence of idiopathic early recurrent pregnancy loss.</p>

scholarly journals Sperm DNA fragmentation in cryopreserved samples from subjects with different cancers

2017 ◽  
Vol 29 (4) ◽  
pp. 637 ◽  
Author(s):  
Lara Tamburrino ◽  
Marta Cambi ◽  
Sara Marchiani ◽  
Ilaria Manigrasso ◽  
Selene Degl'Innocenti ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cancer Patients ◽  
Dna Fragmentation ◽  
Cell Populations ◽  
Sperm Dna Fragmentation ◽  
Ivf Outcome ◽  
Sperm Cryopreservation ◽  
Reproductive Process ◽  
Sperm Dna

Sperm cryopreservation is widely used by cancer patients undergoing chemo- or radiotherapy. Evidence suggests that IVF outcome with cryopreserved spermatozoa from cancer patients is less successful. To determine whether sperm DNA fragmentation (SDF) is involved in the lower fertilising ability of cryopreserved spermatozoa of cancer patients, SDF was evaluated in thawed spermatozoa from 78 men affected by different cancers and 53 men with non-cancer pathologies. SDF was assessed by the terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling (TUNEL), propidium iodide (PI), flow cytometry procedure, which allows determination of two different cell populations (PIbrighter and PIdimmer) and thus to determine the percentage of DNA fragmented sperm in both. PIdimmer spermatozoa are totally unviable, whereas PIbrighter spermatozoa with SDF may be motile and morphologically normal, having higher biological relevance in the reproductive process. We found that the proportion of DNA fragmented PIbrighter cells was significantly higher in thawed spermatozoa from cancer than non-cancer patients. Moreover, a positive correlation was found between the degree of DNA fragmentation and sperm motility in the PIbrighter population of spermatozoa from cancer patients that wasn’t seen in non-cancer patients. The results of the present study suggest that higher SDF levels may contribute to the lower IVF success of cryopreserved spermatozoa from cancer patients and that evaluation of SDF could complement genetic counselling as part of the routine management of cancer patients who seek fertility preservation.

scholarly journals Antioxidant treatment of increased sperm DNA fragmentation: Complex combinations are not more successful

2020 ◽  
Vol 92 (4) ◽  
Author(s):  
Cevahir Ozer
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Single Agent ◽  
Included Study ◽  
Sperm Dna Fragmentation ◽  
Antioxidant Treatment ◽  
Sperm Dna Damage ◽  
Treatment Groups ◽  
Sperm Dna ◽  
Significant Difference

Objective: Oral antioxidant supplementation is part of the treatment of infertility associated with oxidative stress-related sperm damage. It is possible to assume that the combined use of antioxidants will be better than single agent use. The purpose of this study was to compare the effectiveness of different antioxidant combinations in infertile men with increased sperm DNA fragmentation. Materials and methods: We retrospectively reviewed the records of 637 patients who underwent antioxidant support therapy for increased sperm DNA damage between 2014 and 2019. Patients with DNA damage of 30% or more were included study. Result: A total of 163 patients with follow-up data and who fulfilled the study criteria were included in the study. There were four different treatment groups. No statistically significant differences were found between the groups. After 3 months of antioxidant treatment, there was a statistically significant decrease in sperm DNA damage in all treatment groups. However, there was no statistically significant difference between the treatment groups. Conclusions: The complexity of the antioxidant combination may not contribute to the success of the treatment or may cause possible side effects, increase the cost of treatment and decrease patient compliance.

scholarly journals Analysis of sperm DNA fragmentationin infertility patients

2012 ◽  
Vol 61 (3) ◽  
pp. 141-147 ◽  
Author(s):  
Evgeniya Michailovna Shilnikova ◽  
Irina Dmitrievna Fedorova ◽  
Alexander Mkrtichevich Gzgzyan
Keyword(s):  
Dna Fragmentation ◽  
Semen Analysis ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Structural Abnormality ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Rate ◽  
Fragmented Dna ◽  
Sperm Dna ◽  
Infertility Patients

Using Terminal Deoxynucleotidyl Transferase dUTP Nick End Labelling (TUNEL) technique the assessment of the frequency of the spermatozoa with fragmented DNA in the ejaculate from sperm donors and men with infertility was analyzed. The DNA fragmentation rate was higher in spermatozoa of carriers of a chromosomal structural abnormality compared with the control group. There was no correlation between the sperm DNA fragmentation rate and the parameters of semen analysis. The direct linear correlation between the frequency of the spermatozoa with fragmented DNA and vacuole sperm head was found. The DNA fragmentation rate was not correlated to the frequency of the spermatozoa with bulb, amorphous heads or spermatozoa with abnormal acrosome

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