Histological evaluation of electrosurgery and formocresol pulpotomy techniques in primary teeth in dogs

2002 ◽  
Vol 26 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Omar El-Meligy ◽  
Medhat Abdalla ◽  
Sahar El-Baraway ◽  
Magda El-Tekya ◽  
Jeffrey Dean
Keyword(s):  
Histological Examination ◽  
Untreated Control ◽  
Primary Teeth ◽  
Histological Evaluation ◽  
Tissue Reactions ◽  
Pulp Removal ◽  
Histological Reactions ◽  
Surrounding Bone

The purpose of this study was to compare pulpal and periapical tissue reactions to electrosurgery versus formocresol pulpotomy techniques in the primary teeth of dogs. The study was conducted on 33 primary teeth of three mongrel dogs between the ages of one to three months. Each dog had three teeth treated by Formocresol Pulpotomy with Mechanical Coronal Pulp Removal (FC), three teeth treated by Electrosurgery Pulpotomy with Mechanical Coronal Pulp Removal (ES/MCPR), three teeth treated by Electrosurgery Pulpotomy with Electrosurgical Coronal Pulp Removal (ES/ECPR), and two teeth serving as untreated Controls. Dogs one, two and three were sacrificed performing the pulpotomies at two, four and six weeks, respectively. The pulp, periapical tissue and after surrounding bone were submitted to histological examination and the histological reaction was recorded. The results were fourteen out of 18 unfavorable and zero out of three favorable histological reactions occurred in the FC treated teeth. Six out of 18 unfavorable and one out of three favorable histological reactions occurred in the ES/MCPR treated teeth. Nine out of 18 unfavorable and two out of three favorable histological reactions occurred in the ES/ECPR treated teeth. One out of 18 unfavorable and zero out of three favorable histological reactions occurred in the untreated Control teeth. The conclusion of this study is that of the three experimental groups, the teeth treated by Electrosurgery Pulpotomy with either Mechanical or Electrosurgical Coronal Pulp Removal exhibited less histopathological reaction than the teeth treated by Formocresol Pulpotomy.

scholarly journals Prognostic relevance of Ki-67 and P16/INK4a markers in histological diagnosis of cervical dysplasia

2020 ◽  
Vol 7 (1) ◽  
pp. 8-15
Author(s):  
T. A. Dimitriadi ◽  
D. V. Burtsev ◽  
E. A. Dzhenkova ◽  
T. N. Gudtskova ◽  
K. V. Dvadnenko
Keyword(s):  
Histological Examination ◽  
Initial Diagnosis ◽  
Immunohistochemical Method ◽  
Histological Evaluation ◽  
Molecular Profile ◽  
Childbearing Age ◽  
Severe Injuries ◽  
Biopsy Material ◽  
Ki 67 ◽  
P16 Ink4a

Purpose of the study. To determine the quantitative parameters of the molecular markers Ki‑67 and p16/INK4a at CIN 1–3 and to establish the possibility of using them to improve the quality of diagnosis by histological samples.Patients and methods. Biopsy material of the cervix was studied in 71 patients who were carriers of HPV infection. Immunohistochemical method (IHC) in biopsy specimens determined the expression of Ki‑67 and p16/INK4a markers.Results. According to the results of a standard histological examination, the distribution of patientsinto groups occurred as follows: CIN 1–18, in CIN 2–39, in CIN 3–14 patients. It was found that the expression of the studied markers is associated with the severity of dysplastic changes in the tissue of the cervix. By the studied marker, the most characteristic molecular profiles for the degrees of dysplasia were determined: CIN1 – Ki‑67–15–25%, p16/INK4a 10–15%; CIN2 – Ki‑67–70–80%, p16/INK4a – 65–70%; CIN3 – Ki‑67–85–90%, p16/INK4a – 90–95%. Heterogeneity was revealed in the expression of these markersin the CIN 2 group: in 7 cases (22,6%), the molecular profile corresponded to CIN 1, in 1 case (3,6%) to CIN 3. Clinical examples of using the IHC profile to clarify the degree of CIN are given.Conclusion. The use of an IHC study with Ki‑67 and p16/INK4a, in addition to the standard histological examination, makes it possible to objectify the initial diagnosis of the degree of CIN, as well as to identify patients with a high and low risk of developing severe injuries. This is especially true for the CIN2 group, the most problematic in terms of histological evaluation and tactics of clinical management of patients. The use of IHC in the initial diagnosis will contribute to: improving the information content of multiple aimed and loop excisional biopsies; reduction in relapse due to inadequate treatment; eliminating the unreasonable use of such a traumatic method as conization (especially in women of childbearing age).

Validation of ultrasound imaging for Achilles entheseal fibrocartilage in bovines and description of changes in humans with spondyloarthritis

2010 ◽  
Vol 69 (12) ◽  
pp. 2165-2168 ◽  
Author(s):  
Sibel Zehra Aydin ◽  
Emine Bas ◽  
Onur Basci ◽  
Emilio Filippucci ◽  
Richard J Wakefield ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Histological Examination ◽  
Ultrasound Imaging ◽  
Content Validity ◽  
Histological Evaluation ◽  
Healthy Controls ◽  
Ultrasound Machine ◽  
Histological Confirmation ◽  
Masson Trichrome Staining

BackgroundEntheseal fibrocartilage (EF) derangement is hypothesised to be pivotal to the pathogenesis of spondyloarthritis. Ultrasound is useful for visualisation of the enthesis but its role in EF visualisation is uncertain. This work aimed to demonstrate face and content validity of ultrasound for EF visualisation both by bovine histological evaluation and EF imaging in spondyloarthritis.MethodsAchilles enthesis of 18 bovine hindfeet was visualised using a MyLab 70 ultrasound machine. The presence of tissue with EF characteristics was documented and histological confirmation was performed on five randomly selected sections using Masson trichrome staining. Ultrasound of the Achilles tendon (AT) was performed in 19 patients with spondyloarthritis and 21 healthy controls (HC).ResultsThe bovine EF could be visualised in all cases and seen as a thin, uncompressible, well-defined, anechoic layer between the hyperechoic bone and the hyperechoic fibrils of the enthesis both in longitudinal and transverse scans. This region corresponded to EF on histological examination. The same pattern of low signal corresponding to EF location was seen in 17/19 patients and all HC. Discontinuities of the anechoic layer around the erosions and enthesophytes were observed in the spondyloarthritis group. The thickness of the anechoic layer was not significantly different in spondyloarthritis and HC (0.5±0.1 vs 0.5±0.2 mm, p=0.9) whereas the thickness of the EF was greater in men (0.6±0.2 vs 0.5±0.1 mm; p=0.009) compared with women.ConclusionUltrasound can visualise EF of the AT insertion, which can be abnormal in cases of spondyloarthritis. This has implications for a better understanding of enthesopathy.

scholarly journals Histological evaluation of tissue reactions to newly synthetized calcium silicate- and hydroxyapatite-based bioactive materials: in vivo study

2017 ◽  
Vol 145 (7-8) ◽  
pp. 370-377 ◽  
Author(s):  
Vanja Opacic-Galic ◽  
Violeta Petrovic ◽  
Vukoman Jokanovic ◽  
Slavoljub Zivkovic
Keyword(s):  
Inflammatory Reaction ◽  
Calcium Silicate ◽  
Subcutaneous Tissue ◽  
Male Rats ◽  
Histological Evaluation ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Tissue Reactions

Introduction/Objective. Development of materials which could be used as biological bone substitutes is one of the most valuable and active fields of biomaterial research. The goal of the study was to research the reaction of tissue on calcium silicate- (CS) and hydroxyapatitebased (CS-HA) newly synthesized nanomaterials, after being implanted into the subcutaneous tissue of a rats and direct pulp capping of rabbit teeth. Methods. The tested materials were implanted in 40 Wistar male rats, sacrificed after seven, 15, 30, and 60 days. The direct pulp capping was performed on the teeth of rabbits. Cavities were prepared on the vestibular surface of the incisors. The animals were sacrificed after 10 and 15 days. The control material was mineral trioxide aggregate (MTA). Histological analysis covered the tracking of inflammatory reaction cellular components, presence of gigantic cells, and necrosis of the tissue. Results. Seven days after the implantation, the strongest inflammatory response was given by the MTA (3.3 ?} 0.48), while CS and CS-HA scored 3 ? 0.71. After 60 days, the rate of inflammatory reactions dropped, which was the least visible with CS-HA (0.2 ? 0.45). The least visible inflammatory reaction of the rabbits? pulp tissue was spotted with the CS (1.83 ? 0.75), than with the MTA and CS-HA (2.67 ? 1.53, 3 ? 0.63). Conclusion. The newly synthesized materials caused a slight reaction of the subcutaneous tissue. CS-HA showed the best tissue tolerance. Nanostructural biomaterials caused a slight to moderate inflammatory reaction of the rabbits? pulp tissue only in the immediate vicinity of the implanted material.

scholarly journals A histological evaluation of bone calluses in the treatment of tibia fractures in sheep with the use of a semicircular fixator 

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 547-550
Author(s):  
Z. Adamiak ◽  
T. Rotkiewicz
Keyword(s):  
Fracture Healing ◽  
Bone Tissue ◽  
Histological Examination ◽  
Bone Growth ◽  
Compact Bone ◽  
Tibia Fracture ◽  
Histological Evaluation ◽  
Fracture Treatment ◽  
Compact Bone Tissue ◽  
Tibia Fractures

This study discusses the results of a histological examination of bone calluses in the treatment of tibia fractures in sheep with the involvement of a semi-circular fixator. In all sheep, callus samples revealed the presence of well-developed, compact bone tissue in the area of fracture healing. It was found that the use of a semi-circular fixator promoted bone growth, and that it is an effective method for tibia fracture treatment in sheep.

scholarly journals Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ibrahim Hussain ◽  
Keyvan Moharamzadeh ◽  
Ian M. Brook ◽  
Patrício José de Oliveira Neto ◽  
Luiz A. Salata
Keyword(s):  
Histological Examination ◽  
Bone Substitute ◽  
Osteogenic Potential ◽  
Autogenous Bone ◽  
Calvarial Defect ◽  
Defect Model ◽  
Robust Model ◽  
New Zealand White Rabbits ◽  
Ct Data ◽  
Surrounding Bone

The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects.

The Biocompatibility of Silk Scaffold for Tissue Engineered Ligaments

2007 ◽  
Vol 342-343 ◽  
pp. 73-76 ◽  
Author(s):  
Young Kwon Seo ◽  
Gung Min Choi ◽  
Soon Yong Kwon ◽  
Hwa Sung Lee ◽  
Yong Soon Park ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Growth ◽  
Mononuclear Cells ◽  
Inflammatory Responses ◽  
Histological Evaluation ◽  
Artificial Ligament ◽  
Silk Scaffold ◽  
Tissue Reactions

The aim of this study was to estimate the mechanical properties and evaluate the biocompatibility of silk and PGA scaffolds as an artificial ligament to an ACL reconstruction. The scaffold for the artificial ligament was braided / knitted silk or PGA thread. The mechanical properties, cell growth, and subcutaneous tissue reactions were determined for both types of scaffolds. The breaking load of the PGA scaffold was double that of the sericin removed silk scaffold (SRSS). However, the initial attachment and growth of human ACL cells on the SRSS was superior to the PGA scaffold. In addition, the immune response was significantly higher on the PGA scaffold after 72 h (p<0.05) compared with the sericin removed silk scaffold by T lymphocyte and mononuclear cells (MNCs) in vitro cultures. In vivo, the ACL scaffold made from silk or PGA were implanted in the subcutaneous layer in rats and harvested 1 week later. A histological evaluation of the scaffolds explants revealed the presence of monocytes in the SRSS, and an absence of giant cells in all cases. An inflammatory tissue reaction was more conspicuous around the silk scaffold containing sericin and even more around the PGA scaffold compared with SRSS. These results support the conclusion that a properly prepared SRSS, aside from providing benefits in terms of biocompatibility both in vitro and in vivo, can provide suitable scaffolds for the support of ACL cell growth. These results suggest that a SRSS for ACL repair can overcome the current limitations with the PGA scaffold. And SRSS is biocompatible, and the in vitro T cell and MNCs culture model showed inflammatory responses that were comparable to those observed in vivo.

scholarly journals Submucous implant of abrasive dental materials in rat tongues: clinical and histological evaluation

2002 ◽  
Vol 13 (2) ◽  
pp. 103-108 ◽  
Author(s):  
Alexandre Dorneles Pistóia ◽  
Maria Antonia Zancanaro de Figueiredo
Keyword(s):  
Inflammatory Response ◽  
Dental Materials ◽  
Histological Evaluation ◽  
Pumice Stone ◽  
Abrasive Powder ◽  
White Pumice ◽  
Nodular Lesions ◽  
Normal Composition ◽  
Tissue Reactions ◽  
Rat Tongue

This study evaluated the clinical and histological manifestations produced by the submucuous implant of abrasive dental materials in the rat tongue. A total of 128 rats were divided into 4 groups of 32 rats each, according to the material selected for the implant: Herjos-F prophylactic paste both in its normal composition as well as lacking its abrasive components, SS White pumice stone and the abrasive powder of the 3M finishing and polishing sandpaper. The specimens were submitted to clinical and histological analyses at 30, 60, 90 and 120 days. After 90 days, the formation of nodular lesions in animals implanted with materials containing abrasive substances was observed. Histologically, these materials produced marked chronic granulomatous reactions. Herjos-F prophylactic paste produced the greatest reaction. However, without the abrasive components, this paste caused the mildest tissue reactions, with no inflammatory response, that was seen in a progressively greater number of cases after 90 days.

scholarly journals Diffusion-Weighted MRI and FDG-PET in Diagnosis of Endometrial Stromal Nodule

2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Shunsuke Maruyama ◽  
Yukiyasu Sato ◽  
Yumiko Satake ◽  
Hiroko Mise ◽  
Tomoko Kim
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Histological Examination ◽  
Fdg Pet ◽  
Uterine Cavity ◽  
Histological Evaluation ◽  
Low Grade ◽  
Diffusion Weighted Mri ◽  
Node Metastasis ◽  
Diffusion Weighted

Preoperative differentiation of benign endometrial stromal nodule (ESN) from malignant low-grade endometrial sarcoma (LGESS) is challenging, because it requires histological evaluation of the tumor-myometrium interface, which is difficult to obtain in conventional endometrial curettage. A 72-year-old postmenopausal woman presented with 5-year history of persistent vaginal bleeding. Histological examination of the endometrial curettage specimen revealed hyperplasia of apparently normal endometrial stromal cells. T2-weighted magnetic resonance imaging (T2W-MRI) showed polypoid tumor occupying the entire uterine cavity. The tumor exhibited high signal intensity in diffusion-weighted MRI (DW-MRI) and intense accumulation of18F-fluorodeoxyglucose (FDG) in positron emission tomography (PET). Intense FDG accumulation was also observed in the left internal iliac region. Total abdominal hysterectomy, bilateral salpingo-oophorectomy, and pelvic lymphadenectomy were performed under the diagnosis of LGESS with lymph node metastasis. However, postoperative histological examination proved that the tumor was ESN without lymph node metastasis. Since mitotic figure is no longer included in the diagnostic criteria of ESN or LGESS, ESN could exhibit high cellularity and high proliferative activity as observed in this case. Therefore, DW-MRI or FDG-PET is not useful in the differentiation of ESN from LGESS.

scholarly journals CLINICAL, RADIOGRAPHIC, AND HISTOLOGICAL EVALUATION OF THERACAL PULPOTOMY IN HUMAN PRIMARY TEETH

2017 ◽  
Vol 63 (3) ◽  
pp. 2175-2185
Author(s):  
Mariem Wassel ◽  
Dina Amin ◽  
Amira Badran
Keyword(s):  
Primary Teeth ◽  
Histological Evaluation
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