Submucous implant of abrasive dental materials in rat tongues: clinical and histological evaluation

This study evaluated the clinical and histological manifestations produced by the submucuous implant of abrasive dental materials in the rat tongue. A total of 128 rats were divided into 4 groups of 32 rats each, according to the material selected for the implant: Herjos-F prophylactic paste both in its normal composition as well as lacking its abrasive components, SS White pumice stone and the abrasive powder of the 3M finishing and polishing sandpaper. The specimens were submitted to clinical and histological analyses at 30, 60, 90 and 120 days. After 90 days, the formation of nodular lesions in animals implanted with materials containing abrasive substances was observed. Histologically, these materials produced marked chronic granulomatous reactions. Herjos-F prophylactic paste produced the greatest reaction. However, without the abrasive components, this paste caused the mildest tissue reactions, with no inflammatory response, that was seen in a progressively greater number of cases after 90 days.

Download Full-text

Matrix Metalloproteinase-19 is a Predictive Marker for Tumor Invasiveness in Patients with Oropharyngeal Squamous Cell Carcinoma

The International Journal of Biological Markers ◽

10.1177/172460080702200405 ◽

2007 ◽

Vol 22 (4) ◽

pp. 265-273 ◽

Cited By ~ 8

Author(s):

N. Velinov ◽

D. Aebersold ◽

N. Haeni ◽

R. Hlushchuk ◽

F. Weinstein ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Inflammatory Response ◽

Matrix Metalloproteinase ◽

Cell Carcinoma ◽

Squamous Cell ◽

Oropharyngeal Squamous Cell Carcinoma ◽

Histological Evaluation ◽

Invasive Front ◽

Tumor Invasiveness ◽

Nuclear Polymorphism

Aims To evaluate the expression of matrix metalloproteinase-19 (MMP-19) in oropharyngeal squamous cell carcinoma along with its association with structural features of invasiveness. To investigate whether MMP-19 expression correlates with lymphatic or systemic metastasis and prognosis in patients who have received definitive radiotherapy. Methods and results The histological evaluation of the invasive front was based on Bryne's malignancy grading system. We correlated the immunohistochemical expression pattern with morphological parameters which characterize tumor invasiveness such as keratinization, nuclear polymorphism, invasion pattern, and the host inflammatory response. Local immunoreactivity for MMP-19 was positively correlated with tumor invasiveness as reflected in its structural characteristics and the degree of nuclear polymorphism, and negatively correlated with the inflammatory response of the host. No correlation existed between MMP-19 expression and clinicopathological features (TNM stage, grade of differentiation) or a patient's outcome and prognosis. Conclusions This latter finding probably reflects the unique change for MMPs from high immunoreactivity within healthy tissue areas and non-invasive tumor parts, through absence in the least invasive neoplastic regions, to strong re-expression at a highly invasive front of the same tumor. Our findings indicate that MMP-19 can be used as a marker for tumor invasiveness in patients with oropharyngeal squamous cell carcinoma.

Download Full-text

Does hypersensitive teeth show pulp inflammation?

RGO - Revista Gaúcha de Odontologia ◽

10.1590/1981-86372019000113580 ◽

2019 ◽

Vol 67 ◽

Author(s):

Daniele Paraguassú FAGUNDES-DE-SOUZA ◽

Marcelo Henrique NAPIMOGA ◽

Andresa Borges SOARES ◽

Vera Cavalcanti ARAÚJO ◽

Cecilia Pedroso TURSSI

Keyword(s):

Inflammatory Response ◽

Dental Pulp ◽

Mineral Water ◽

Sagittal Plane ◽

Soft Drink ◽

Histological Evaluation ◽

Dentin Hypersensitivity ◽

Pulp Tissue ◽

Pulp Inflammation ◽

Significant Difference

ABSTRACT Objective: This study investigated the presence of inflammatory response in the dental pulp of rats showing hypersensitive dentin, induced by erosive episodes. Methods: Sixteen Wistar rats were fed with commercial sucrose-free pellet diet for 12 hours; whereas the food was removed during the remainder of the day, and the animals received mineral water or a lemon-based sucrose-free soft drink, according to the group to which they belonged. Eight animals consumed the soft drink to induce hypersensitivity, while the other 8 animals received mineral water (control). After six weeks, the animals were euthanized, the mandible was removed and subjected to a median incision in the sagittal plane, to obtain right and left hemimandibles. The slides stained with hematoxylin-eosin were analyzed using light microscopy. Results: Histological evaluation of the control and experimental groups revealed no inflammatory process in the pulp tissue, and the presence of inflammatory cells, such as lymphocytes, plasma cells, eosinophils and macrophages, was not observed. In addition, there was no edema or dilated and congested blood vessels. The Mann-Whitney test showed no significant difference (p = 1.000) between the experimental and the control groups. Conclusion: In the animal model used, dentin hypersensitivity does not trigger dental pulp inflammatory response.

Download Full-text

Hybrid Chitosan Membranes Tested in Sheep for Guided Tissue Regeneration

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.1265 ◽

2007 ◽

Vol 361-363 ◽

pp. 1265-1268 ◽

Cited By ~ 3

Author(s):

P.P. Cortez ◽

Yuki Shirosaki ◽

C.M. Botelho ◽

M.J. Simões ◽

F. Gartner ◽

...

Keyword(s):

Inflammatory Response ◽

Tissue Regeneration ◽

Chitosan Solution ◽

Clinical Applications ◽

Histological Evaluation ◽

Sheep Model ◽

The Third ◽

Chitosan Membranes

Previous in vitro studies confirmed an improved cytocompatibility of chitosan-silicate hybrid membranes over chitosan membranes. The main goal of this study was to assess the in vivo histocompatibility of both membranes through subcutaneous implantations at different time periods, 1 week, 1, 2 and 3 months, using a sheep model. Chitosan membranes elicited an exuberant inflammatory response and were consequently rejected. The hybrid chitosan membranes were not rejected and the degree of inflammatory response decreased gradually until the third month of implantation. Histological evaluation also showed that these membranes can be resorbed in vivo. This study demonstrates that the incorporation of silicate into the chitosan solution improves its histocompatibility, indicating that the hybrid chitosan-silicate membranes are suitable candidates to be used in clinical applications.

Download Full-text

Impression Material Mass Retained in the Mucobuccal Fold

Case Reports in Dentistry ◽

10.1155/2014/416965 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5

Author(s):

N. Karam Genno ◽

A. Assaf

Keyword(s):

Foreign Body ◽

Oral Cavity ◽

Case History ◽

Foreign Bodies ◽

Dental Materials ◽

Spectrometric Analysis ◽

Mucosal Tissue ◽

Impression Material ◽

Foreign Materials ◽

Tissue Reactions

Trapped foreign bodies and tissue reactions to foreign materials are commonly encountered in the oral cavity. Traumatically introduced dental materials, instruments, or needles are the most common materials referred to in the dental literature. This paper describes an iatrogenic foreign body encapsulation in the oral mucosa, clinically appearing as5×10 mm tumor-like swelling with an intact overlying epithelium and diagnosed as a polymeric impression material. Detailed case history and, clinical and radiographic examinations including CBCT and spectrometric analysis of the retrieved sample were necessary to determine accurately the nature, size, and location of the foreign body. It is suggested that the origin of the material relates to an impression made 2 years ago, leaving a mass trapped in a traumatized mucosal tissue.

Download Full-text

Iatrogenic Damage to the Periodontium by Chemicals and Dental Materials

The Open Dentistry Journal ◽

10.2174/1874210601509010223 ◽

2015 ◽

Vol 9 (1) ◽

pp. 223-227 ◽

Cited By ~ 1

Author(s):

Biju Justus ◽

Syed Sirajuddin ◽

Veenadharini Gundapaneni ◽

Shriparna Biswas ◽

Kumuda MN ◽

...

Keyword(s):

Dental Materials ◽

Federal Agencies ◽

Biological Interaction ◽

Iatrogenic Damage ◽

Standard Practices ◽

Tissue Reactions

The toxicity and tissue reactions to dental materials are receiving more attention as a wide variety of materials are used and as federal agencies demonstrate more concern in this area. A further indication of the importance of the interaction of materials and tissues is the development of recommended standard practices and tests for the biological interaction of materials.

Download Full-text

Inflammatory response of fibroblasts stimulated by dental materials

Dental Materials ◽

10.1016/j.dental.2014.08.289 ◽

2014 ◽

Vol 30 ◽

pp. e141-e142

Author(s):

K.C.S. Modena ◽

M.P. Calabria ◽

C.N. Pegoraro ◽

M.C. Veronezi ◽

C.R. Sipert ◽

...

Keyword(s):

Inflammatory Response ◽

Dental Materials

Download Full-text

Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats

Polymers ◽

10.3390/polym13162684 ◽

2021 ◽

Vol 13 (16) ◽

pp. 2684

Author(s):

Charlotte Koppe ◽

Andreas Hoene ◽

Uwe Walschus ◽

Birgit Finke ◽

Holger Testrich ◽

...

Keyword(s):

T Lymphocytes ◽

Inflammatory Response ◽

Discharge Plasma ◽

Thin Film Deposition ◽

Film Deposition ◽

Rf Discharge ◽

Local Inflammatory Response ◽

Regulatory T Lymphocytes ◽

Fluorocarbon Polymer ◽

Tissue Reactions

Orthopaedic implants and temporary osteosynthesis devices are commonly based on Titanium (Ti). For short-term devices, cell-material contact should be restricted for easy removal after bone healing. This could be achieved with anti-adhesive plasma-fluorocarbon-polymer (PFP) films created by low-temperature plasma processes. Two different PFP thin film deposition techniques, microwave (MW) and radiofrequency (RF) discharge plasma, were applied to receive smooth, hydrophobic surfaces with octafluoropropane (C3F8) or hexafluorohexane (C6F6) as precursors. This study aimed at examining the immunological local tissue reactions after simultaneous intramuscular implantation of four different Ti samples, designated as MW-C3F8, MW-C6F6, RF-C3F8 and Ti-controls, in rats. A differentiated morphometric evaluation of the inflammatory reaction was conducted by immunohistochemical staining of CD68+ macrophages, CD163+ macrophages, MHC class II-positive cells, T lymphocytes, CD25+ regulatory T lymphocytes, NK cells and nestin-positive cells in cryosections of surrounding peri-implant tissue. Tissue samples were obtained on days 7, 14 and 56 for investigating the acute and chronical inflammation (n = 8 rats/group). Implants with a radiofrequency discharge plasma (RF-C3F8) coating exhibited a favorable short- and long-term immune/inflammatory response comparable to Ti-controls. This was also demonstrated by the significant decrease in pro-inflammatory CD68+ macrophages, possibly downregulated by significantly increasing regulatory T lymphocytes.

Download Full-text

Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9-1 using subcuticular technique in pigs

10.21203/rs.2.14166/v2 ◽

2019 ◽

Author(s):

Tanja Plavec ◽

Tanja Švara ◽

Irena Zdovc ◽

Mitja Gombač ◽

Marija Damjanovska ◽

...

Keyword(s):

Inflammatory Response ◽

Suture Material ◽

Giant Cells ◽

Tissue Reaction ◽

Absorbable Suture ◽

Suture Materials ◽

Tissue Samples ◽

Trueperella Pyogenes ◽

Cumulative Score ◽

Tissue Reactions

Abstract Background: Glycomer 631 and lactomer 9-1 are absorbable suture materials indicated for soft tissue approximation in non-infected wounds. Pigs are often used as surgical models in translational research, however, the reports of tissue reactions to both suture materials in pigs are lacking. The aim of this study was to assess clinical and histologic responses of skin incisions closed with subcuticular technique using glycomer 631 and lactomer 9-1 in pigs. Results: Skin incisions of 17 pigs were closed with glycomer 631 and lactomer 9-1 and clinical reactive score (CRS) including erythema, swelling, discharge, and dehiscence was calculated on postoperative days 7 and 14. Subcuticular tissue reaction was assessed histologically on postoperative day 14 (the presence of extravascular neutrophils, macrophages, multinucleated giant cells, lymphocytes, fibroblasts, bacterial colonies, overall severity of inflammatory response to suture material), and the cumulative score of variables calculated as aggregate tissue irritation score (ATIS). Tissue samples were examined for suture extrusion and evaluated microbiologically. Clinical reactive score did not differ between the suture materials. Only one variable of ATIS, overall severity of inflammatory response, was lower ( p = 0.029) when glycomer 631 was used. Suture extrusion was found in 10/17 of incisions closed by glycomer 631 and in 7/13 of incisions closed by lactomer 9-1. Trueperella pyogenes was isolated from the skin and from the area of tissue reaction in six pigs. Conclusions: No difference in CRS between the suture materials was observed. Glycomer 631 induced less tissue reaction only in terms of overall severity of inflammatory response. Suture extrusion was observed in more than 50% of incisions regardless of the suture material. Trueperella pyogenes was the only pathogen isolated from the tissue surrounding the suture material.

Download Full-text

Histological evaluation of tissue reactions to newly synthetized calcium silicate- and hydroxyapatite-based bioactive materials: in vivo study

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh160719063o ◽

2017 ◽

Vol 145 (7-8) ◽

pp. 370-377 ◽

Cited By ~ 1

Author(s):

Vanja Opacic-Galic ◽

Violeta Petrovic ◽

Vukoman Jokanovic ◽

Slavoljub Zivkovic

Keyword(s):

Inflammatory Reaction ◽

Calcium Silicate ◽

Subcutaneous Tissue ◽

Male Rats ◽

Histological Evaluation ◽

Direct Pulp Capping ◽

Pulp Tissue ◽

Pulp Capping ◽

Tissue Reactions

Introduction/Objective. Development of materials which could be used as biological bone substitutes is one of the most valuable and active fields of biomaterial research. The goal of the study was to research the reaction of tissue on calcium silicate- (CS) and hydroxyapatitebased (CS-HA) newly synthesized nanomaterials, after being implanted into the subcutaneous tissue of a rats and direct pulp capping of rabbit teeth. Methods. The tested materials were implanted in 40 Wistar male rats, sacrificed after seven, 15, 30, and 60 days. The direct pulp capping was performed on the teeth of rabbits. Cavities were prepared on the vestibular surface of the incisors. The animals were sacrificed after 10 and 15 days. The control material was mineral trioxide aggregate (MTA). Histological analysis covered the tracking of inflammatory reaction cellular components, presence of gigantic cells, and necrosis of the tissue. Results. Seven days after the implantation, the strongest inflammatory response was given by the MTA (3.3 ?} 0.48), while CS and CS-HA scored 3 ? 0.71. After 60 days, the rate of inflammatory reactions dropped, which was the least visible with CS-HA (0.2 ? 0.45). The least visible inflammatory reaction of the rabbits? pulp tissue was spotted with the CS (1.83 ? 0.75), than with the MTA and CS-HA (2.67 ? 1.53, 3 ? 0.63). Conclusion. The newly synthesized materials caused a slight reaction of the subcutaneous tissue. CS-HA showed the best tissue tolerance. Nanostructural biomaterials caused a slight to moderate inflammatory reaction of the rabbits? pulp tissue only in the immediate vicinity of the implanted material.

Download Full-text