scholarly journals Fluoride Release from Fluoride Varnishes under Acidic Conditions

2014 ◽  
Vol 39 (1) ◽  
pp. 35-39 ◽  
Author(s):  
F Lippert
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Fluoride Varnish ◽  
Laboratory Research ◽  
Fluoride Release ◽  
Acidic Solutions ◽  
Acid Ph ◽  
Opposite Behavior ◽  
Acidic Conditions

Objective: The aim was to investigate the in vitro fluoride release from fluoride varnishes under acidic conditions. Study design: Poly(methyl methacrylate) blocks (Perspex, n=3 per group) were painted with 80±5 mg fluoride varnish (n=10) and placed into artificial saliva for 30min. Then, blocks were placed into either 1% citric acid (pH 2.27) or 0.3% citric acid (pH 3.75) solutions (n=3 per solution and varnish) for 30min with the solutions being replaced every 5min. Saliva and acid solutions were analyzed for fluoride content. Data were analyzed using three-way ANOVA (varnish, solution, time). Results: The three-way interaction was significant (p>0.0001). Fluoride release and release patterns varied considerably between varnishes. Fluoride release in saliva varied by a factor of more than 10 between varnishes. Some varnishes (CavityShield, Nupro, ProFluorid, Vanish) showed higher fluoride release in saliva than during the first 5min of acid exposure, whereas other varnishes (Acclean, Enamel-Pro, MI Varnish, Vella) showed the opposite behavior. There was little difference between acidic solutions. Conclusions: Fluoride release from fluoride varnishes varies considerably and also depends on the dissolution medium. Bearing in mind the limitations of laboratory research, the consumption of acidic drinks after fluoride varnish application should be avoided to optimize the benefit/risk ratio.

scholarly journals Effects on Dentin Treated with Eluted Multi-Mineral Varnish In Vitro

2012 ◽  
Vol 6 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Robert L Karlinsey ◽  
Allen C Mackey ◽  
Craig S Schwandt
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Control Group ◽  
Distilled Water ◽  
Water Control ◽  
Acid Ph ◽  
Ph Cycling ◽  
Demineralized Dentin ◽  
Acid Challenge

Purpose: The purpose of this pilot study was to assess the non-contact effects of a NaF varnish on the morphology and elemental compositions of sound and demineralized dentin in an in vitro pH cycling model. Methods: 3 mm diameter dentin cores were extracted from bovine teeth, mounted in acrylic rods, and ground and polished. Sound and demineralized (in 10 mL unstirred 50% citric acid (pH = 1.2) for two minutes) specimens were divided into four groups (N=6) corresponding to treatments with either water (control group) or non-contact Vanish® 5% NaF White Varnish with tri-calcium phosphate (3M ESPE). For the non-contact varnish group, one 0.50 ml unit-dose was applied to acrylic rods and positioned in the same stopper as three dentin specimens. The four groups were then cycled in a regimen consisting of three rounds of one-hour treatments with each round followed by immersion in a three-minute static acid challenge (0.3% citric acid, pH = 3.8). For each one-hour treatment immersion the test groups were immersed in distilled water. The specimens were thoroughly rinsed with distilled water after each treatment and after each acid challenge. After the last challenge, specimens were rinsed with distilled water and immersed in artificial saliva (AS) overnight. All treatments, acid challenges and immersion in AS solution were performed in an incubator set at 37°C. After three days, the specimens were assessed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Results: SEM and EDS results demonstrated the non-contact varnish group can provide mineralization benefits relative to the control group for both sound and demineralized dentin substrates. Depositions from mineral eluting from the varnish developed on the intertubular and intratubular regions alike, with the most prominent depositions appearing on initially demineralized dentin. Conclusions: In this observational study, acid-resistant mineral depositions were produced on the sound and dentin surfaces without direct application from a multi-mineral varnish containing fluoride, calcium and phosphate.

The Impact of the Demineralized Organic Matrix on the Effect of TiF4 Varnish on the Progression of Dentin Erosive Loss

2017 ◽  
Vol 51 (3) ◽  
pp. 264-270 ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mariele Vertuan ◽  
Marília Afonso Rabelo Buzalaf ◽  
Ana Carolina Magalhães
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Organic Matrix ◽  
Analysis Data ◽  
Acid Ph ◽  
Bonferroni Test ◽  
And Control ◽  
The Impact

This in vitro study compared the effect of TiF4 varnish with that of NaF varnish, applied on pre-eroded bovine dentin samples, with respect to the progression of erosive loss, in the presence or absence of the demineralized organic matrix (DOM). One hundred and sixty bovine dentin samples were pre-eroded (0.1% citric acid, pH 2.5, 30 min). Half of the samples were subjected to the DOM removal (collagenase solution, 5 days). Samples with and without the DOM were treated according to the groups (n = 20 with DOM and 20 without DOM/group): TiF4 varnish (2.45% F), NaF varnish (2.45% F), placebo varnish (without fluoride) and control (no treatment). Thereafter, the treated samples were submitted to erosive challenges 4 × 90 s/day (0.1% citric acid, pH 2.5) during 7 days. Between the challenges, the samples were immersed in artificial saliva. The dentin erosive loss was measured using contact profilometry (µm, n = 15). Five dentin samples per group were prepared for energy-dispersive X-ray spectroscopy analysis. Data were compared using 2-way ANOVA/Bonferroni test (p < 0.05). Both fluoride varnishes were effective in reducing the erosive loss progression regardless of the dentin condition when compared to placebo varnish and control groups. Despite the fact that the TiF4 varnish was more effective than the NaF varnish for both dentin conditions (p < 0.001), its effect was significantly reduced in the absence of DOM (p < 0.05). It can be concluded that the TiF4 varnish is the best treatment in reducing the progression of dentin erosive loss (100%) in vitro, but its protective effect is more pronounced in the presence of DOM.

scholarly journals Comparative Evaluation of Fluoride Release From Chemically Cured and Light-Cured Orthodontic Bonding Agents and Surface Alteration of Enamel: An in vitro Study

2020 ◽  
Vol 54 (3) ◽  
pp. 233-239
Author(s):  
Ashish Kumar Barik ◽  
Ritu Duggal
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Fluoride Content ◽  
Bonding Agent ◽  
Fluoride Release ◽  
Bonding Agents ◽  
Surface Alteration ◽  
Orthodontic Bonding ◽  
Group B

Introduction: Fluoride is known to reduce demineralization and enhance remineralization which is desirable around orthodontic brackets. Material and methods: This study was carried out to determine the rate of fluoride release, fluoride content of enamel, and surface alteration of enamel from orthodontic bonding agents. Thirty extracted maxillary first premolars were divided into 2 groups of 15 teeth each. Group A—teeth were bonded with a chemically cured orthodontic bonding agent containing fluoride (Rely-a-Bond, Reliance, Itasca) and group B—teeth were bonded with a light-cured orthodontic bonding agent containing fluoride (Light Bond Reliance, Itasca). All individual sample teeth were used to estimate fluoride release and fluoride content of enamel. The same samples were also used to evaluate enamel surface alterations. The rate of fluoride release from the above 2 orthodontic bonding agents were measured in artificial saliva on day 1, 2, 3, 4, 5, 6, 7, 14, 30, 60, and 90. The fluoride content of the enamel was evaluated on day “0” and day “90” of bonding. The samples were also evaluated for the surface changes in enamel using scanning electron microscope (SEM). Results: The results showed that (a) chemically cured and light-cured bonding agent showed a dramatic decrease in the concentration of fluoride release from day “1” to day “2,” (b) the concentration of fluoride release kept decreasing from day “1” to day “90” of measurement in both the groups, and (c) SEM of the bracket margins bonded with chemically cured bonding agent and light-cured bonding agent revealed particle depositions of irregular shape and size on day “90.” Conclusion: Light-cured bonding agent (Light Bond) released higher concentration of fluoride as compared to chemically cured bonding agent (Rely-a-Bond). Light-cured bonding agent (Light Bond) showed higher increase in fluoride content of enamel than chemically cured bonding agent (Rely-a-Bond).

scholarly journals Comparison of the Fluoride Release of Silver Diamine Fluoride, Fluoride Varnish, Acidulated Phosphate Fluoride Gel on Extracted Teeth over Various Time Intervals in Artificial Saliva

2021 ◽  
pp. 55-63
Author(s):  
Taniya Thakur ◽  
Pratik Kumar Lahiri ◽  
Madhumanti Karmakar ◽  
Bhumi Sarvaiya ◽  
Piyali Datta ◽  
...  
Keyword(s):  
Tap Water ◽  
Artificial Saliva ◽  
Fluoride Concentration ◽  
Maximum Amount ◽  
Fluoride Varnish ◽  
Fluoride Ion ◽  
Fluoride Release ◽  
Silver Diamine Fluoride ◽  
Group 4 ◽  
Group 2

Silver Diamine Fluoride (SDF) has been documented to effectively arrest dental caries and reduce dental hypersensitivity. SDF promotes remineralization and harden the carious lesion. SDF increases fluoride concentration in saliva and increase the bioavailability of fluoride in saliva. After SDF application, fluoride ion promotes remineralization and silver ion is available for antimicrobial action. Aim: The study aims to determine and compare the amount of fluoride released from various fluoride releasing materials in artificial saliva after 24 hours, 7th day and 14th day of the study. Materials and Methods: 96 premolars free of any caries, fractures, or any other defects were sterilized in 10% formalin for 2 weeks. Then they were rinsed in tap water to remove any fixative from its surface and then stored in deionised distilled water for a period of 30 days prior to testing. Tooth samples were divided into four groups- Group 1: 38% SDF, Group 2: 1.23% APF gel, Group 3: Fluoride varnish and Group 4: Control. All the teeth specimens were blot dried and subjected to their respective material. Fluoride release was analysed using a Fluoride ion-selective electrode after 24 hours, 7days and 14 days of suspension in artificial saliva. Results: Mann-Whitney U Test for inter-group comparison was used for statistical evaluation. 24 Hours fluoride release: The maximum amount of fluoride was released from Fluoride Varnish followed by SDF then APF Gel and least by artificial saliva alone (control) (p<0.001). 7th Day fluoride release: The maximum amount of fluoride was released from SDF followed by Fluoride Varnish then APF Gel and least by artificial saliva alone (control) (p<0.001). 14th Day fluoride release: The maximum amount of fluoride was released from Fluoride Varnish followed by SDF then APF Gel and least by artificial saliva alone (control) (p<0.001).

scholarly journals An In Vitro Investigation of Anticaries Efficacy of Fluoride Varnishes

2019 ◽  
Vol 44 (5) ◽  
pp. E234-E243 ◽  
Author(s):  
L Al Dehailan ◽  
EA Martinez-Mier ◽  
GJ Eckert ◽  
F Lippert
Keyword(s):  
Artificial Saliva ◽  
Acid Resistance ◽  
Fluoride Release ◽  
In Vitro Investigation ◽  
Ph Cycling ◽  
Baseline Values ◽  
Bovine Enamel ◽  
Early Caries ◽  
Caries Lesions

SUMMARY Most currently marketed fluoride varnishes (FVs) have not been evaluated for their effectiveness in preventing dental caries. The objective of this study was to investigate the anticaries efficacy, measured as fluoride release into artificial saliva (AS); change in surface microhardness of early enamel caries lesions; and enamel fluoride uptake (EFU) of 14 commercially available FVs and two control groups. Bovine enamel specimens (5×5 mm) were prepared and assigned to 18 groups (n=12). Early caries lesions were created in the specimens and characterized using Vickers microhardness (VHNlesion). FV was applied to each group of specimens. Immediately afterward, specimens were incubated in 4 mL of AS for 18 hours, which were collected and renewed every hour for the first six hours. AS samples were analyzed for fluoride using an ion-specific electrode. Specimens were then brushed for 20 seconds with toothpaste slurry and subjected to pH cycling consisting of a four-hour/day acid challenge and one-minute treatments with 1100 ppm F dentifrice for five days. Microhardness was measured following pH cycling (VHNpost). EFU was determined using microbiopsy. Acid resistance (eight-hour demin challenge) was performed after pH cycling, and microhardness was measured (VHNart) and compared with baseline values to test the FV impact after pH cycling. One-way analysis of variance was used for data analysis (α=0.05). FVs differed in their release characteristics (mean ± SD ranged from 14.97 ± 2.38 μg/mL to 0.50 ± 0.15 μg/mL), rehardening capability (mean ± SD ranged from 24.3 ± 15.1 to 11.7 ± 12.7), and ability to deliver fluoride to demineralized lesions (mean ± SD ranged from 3303 ± 789 μg/cm3 to 707 ± 238 μg/cm3). Statistically significant but weak linear associations were found between ΔVHN(post – lesion), EFU, and fluoride release (correlations 0.21-0.36). The results of this study demonstrated that differences in FV composition can affect their efficacy in in vitro conditions.

In vitro fluoride release from restorative materials in water versus artificial saliva medium (SAGF)

1998 ◽  
Vol 14 (6) ◽  
pp. 441-447 ◽  
Author(s):  
Bernard Levallois ◽  
Yannick Fovet ◽  
Luc Lapeyre ◽  
Jean Yves Gal
Keyword(s):  
Artificial Saliva ◽  
Fluoride Release ◽  
Restorative Materials

Effect of a Single Application of TiF4 Varnish versus Daily Use of a Low-Concentrated TiF4/NaF Solution on Tooth Erosion Prevention in vitro

2016 ◽  
Vol 50 (5) ◽  
pp. 462-470 ◽  
Author(s):  
Ana Carolina Magalhães ◽  
Milena Gargaro dos Santos ◽  
Livia Picchi Comar ◽  
Marília Afonso Rabelo Buzalaf ◽  
Carolina Ganss ◽  
...  
Keyword(s):  
Citric Acid ◽  
Protective Effect ◽  
Tooth Loss ◽  
Artificial Saliva ◽  
Tooth Erosion ◽  
Single Application ◽  
Root Dentin ◽  
Bovine Enamel ◽  
Erosion Prevention

This study investigated the isolated and combined effect of a single application of TiF4 or NaF varnish versus daily use of a solution containing a low concentration of TiF4/NaF against tooth erosion in vitro. A total of 90 bovine enamel and 108 root dentin samples were treated as follows: control (no treatment), solution containing TiF4/NaF (500 ppm F-, pH 4.4), NaF varnish (24,500 ppm F-, pH 5.0), TiF4 varnish (24,500 ppm F-, pH 1.0), TiF4 varnish + solution, and NaF varnish + solution. The erosive challenges were performed 4 × 90s/day (0.1% citric acid, pH 2.5) and, between them, the samples were immersed in artificial saliva. The tooth loss was measured using contact profilometry (after 7 days for dentin and after 7, 10, and 14 days for enamel). All treatments were effective in reducing tooth loss, except NaF varnish for enamel on day 7 (p < 0.0001). TiF4/NaF solution and TiF4 varnish did not differ with respect to enamel loss for 10 days; thereafter, TiF4 varnish lost its protective effect compared to TiF4/NaF solution. The combination of vehicles was more effective in reducing enamel loss than both varnishes on their own but not compared to the solution. For dentin, TiF4 varnish was more effective than NaF varnish, while TiF4/NaF solution and NaF varnish were similar. The combination of vehicles improved the protective effect only when compared to NaF varnish on its own (p < 0.0001). Both types of TiF4 applications, isolated or combined, were effective against tooth erosion, but some differences in their performance were seen between enamel and dentin.

scholarly journals Profile of Fluoride Release from a Nanohybrid Composite Resin

2015 ◽  
Vol 3 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Raquel Assed Bezerra Silva ◽  
Fernanda Regina Ribeiro Santos ◽  
Augusto Cesar Cropanese Spadaro ◽  
Ana Cristina Morseli Polizello ◽  
Andiara De Rossi ◽  
...  
Keyword(s):  
Composite Resin ◽  
Artificial Saliva ◽  
Glass Ionomer Cement ◽  
Positive Control ◽  
Negative Control ◽  
Fluoride Release ◽  
Fluoride Ions ◽  
Significance Level ◽  
Nanohybrid Composite

The aim of this study was to evaluate in vitro the amount and profile of fluoride release from a fluoride-containing nanohybrid composite resin (Tetric® N-Ceram) by direct potentiometry. Thirty specimens (5 mm diameter x 3 mm high; n=10/material) were made of Tetric® N-Ceram, Vitremer® resin-modified glass ionomer cement (RMGIC) (positive control) or Filtek® Z350 nanofill composite resin (negative control). The specimens were stored individually in plastic tubes containing 1 mL of artificial saliva at 37°C, which was daily renewed during 15 days. At each renewal of saliva, the amount of fluoride ions released in the solution was measured using a fluoride ion-selective electrode with ion analyzer, and the values obtained in mV were converted to ppm (µg/mL). Data were analyzed statistically by ANOVA and Tukey’s post-hoc test at a significance level of 5%. The results showed that the resins Tetric® N-Ceram and Filtek® Z350 did not release significant amounts of fluoride during the whole period of evaluation (p>0.05). Only Vitremer® released significant amounts of fluoride ions during the 15 days of the experiment, with greater release in first 2 days (p<0.05) and stabilization in the subsequent days (p>0.05). In conclusion, the nanohybrid composite resin Tetric® N-Ceram did not present in vitro fluoride-releasing capacity throughout the 15 days of study.

Frequency of Application of AmF/NaF/SnCl2 Solution and Its Potential in Controlling Human Enamel Erosion Progression: An in situ Study

2017 ◽  
Vol 51 (2) ◽  
pp. 141-148 ◽  
Author(s):  
C.V. da Silva ◽  
T.M. Ramos-Oliveira ◽  
T.F. Mantilla ◽  
P.M. de Freitas
Keyword(s):  
Citric Acid ◽  
Morphological Changes ◽  
Treatment Protocol ◽  
Negative Control ◽  
Tissue Loss ◽  
In Situ Study ◽  
Acid Ph ◽  
Human Enamel

Although several studies have demonstrated the efficacy of AmF/NaF/SnCl2 solution in inhibiting dental erosion progression, measures for further improvement in its effectiveness are paramount. Thus, this in situ study evaluated whether the protective effect promoted by the AmF/NaF/SnCl2 solution would be enhanced by increasing its frequency of use. The study was conducted with 12 volunteers, a 4-phase (5 days each) randomized, crossover model. Extraoral erosive challenges (0.5% citric acid, pH 2.6, 6 × 2 min/day) and rinsing protocol (1 or 2 × 2 min/day) were performed. Before the in situ phase, human enamel samples were subjected to an in vitro surface softening (1% citric acid, pH 4.0, for 3 min). Four treatment protocols were tested using samples in replicas (n = 12): group G1 - deionized water (negative control); G2 - NaF solution (positive control, 500 ppm F-, pH 4.5); G3 - AmF/NaF/SnCl2 solution (500 ppm F-, 800 ppm Sn2+, pH 4.5) once a day; G4 - AmF/NaF/SnCl2 solution twice a day. Tissue loss and morphological changes were determined by optical profilometry (n = 12) and scanning electron microscopy (n = 3) analysis, respectively. Data were statistically analyzed by ANOVA with subsequent pairwise comparison of treatments. Tissue loss means (±SD in µm) for each treatment protocol and statistical differences were found as follows: G1 4.55 ± 2.75, G2 4.59 ± 2.13, G3 2.64 ± 1.55, and G4 1.34 ± 1.16. Although there was no difference between the 2 AmF/NaF/SnCl2 solution application regimens (once or twice a day), application of the product twice a day was the only treatment that was able to control erosion progression, differing from the control groups.

scholarly journals Fluoride release from fluoride varnish under in vitro and in vivo conditions

2017 ◽  
Vol 54 (4) ◽  
pp. 327-331 ◽  
Author(s):  
Dagmara Piesiak-Pańczyszyn ◽  
Urszula Kaczmarek
Keyword(s):  
Fluoride Varnish ◽  
Fluoride Release
Sign in / Sign up

Export Citation Format

Share Document