Frequency of Application of AmF/NaF/SnCl2 Solution and Its Potential in Controlling Human Enamel Erosion Progression: An in situ Study

2017 ◽  
Vol 51 (2) ◽  
pp. 141-148 ◽  
Author(s):  
C.V. da Silva ◽  
T.M. Ramos-Oliveira ◽  
T.F. Mantilla ◽  
P.M. de Freitas
Keyword(s):  
Citric Acid ◽  
Morphological Changes ◽  
Treatment Protocol ◽  
Negative Control ◽  
Tissue Loss ◽  
In Situ Study ◽  
Acid Ph ◽  
Human Enamel

Although several studies have demonstrated the efficacy of AmF/NaF/SnCl2 solution in inhibiting dental erosion progression, measures for further improvement in its effectiveness are paramount. Thus, this in situ study evaluated whether the protective effect promoted by the AmF/NaF/SnCl2 solution would be enhanced by increasing its frequency of use. The study was conducted with 12 volunteers, a 4-phase (5 days each) randomized, crossover model. Extraoral erosive challenges (0.5% citric acid, pH 2.6, 6 × 2 min/day) and rinsing protocol (1 or 2 × 2 min/day) were performed. Before the in situ phase, human enamel samples were subjected to an in vitro surface softening (1% citric acid, pH 4.0, for 3 min). Four treatment protocols were tested using samples in replicas (n = 12): group G1 - deionized water (negative control); G2 - NaF solution (positive control, 500 ppm F-, pH 4.5); G3 - AmF/NaF/SnCl2 solution (500 ppm F-, 800 ppm Sn2+, pH 4.5) once a day; G4 - AmF/NaF/SnCl2 solution twice a day. Tissue loss and morphological changes were determined by optical profilometry (n = 12) and scanning electron microscopy (n = 3) analysis, respectively. Data were statistically analyzed by ANOVA with subsequent pairwise comparison of treatments. Tissue loss means (±SD in µm) for each treatment protocol and statistical differences were found as follows: G1 4.55 ± 2.75, G2 4.59 ± 2.13, G3 2.64 ± 1.55, and G4 1.34 ± 1.16. Although there was no difference between the 2 AmF/NaF/SnCl2 solution application regimens (once or twice a day), application of the product twice a day was the only treatment that was able to control erosion progression, differing from the control groups.

Optimizing citric acid protocol to control implant‐related infections: An in vitro and in situ study

2021 ◽  
Author(s):  
Jairo M. Cordeiro ◽  
Júlia M. Pires ◽  
João G. S. Souza ◽  
Carolina V. Lima ◽  
Martinna M. Bertolini ◽  
...  
Keyword(s):  
Citric Acid ◽  
In Situ Study

The Erosive Potential of Different Malt Drinks: An in vitro and in situ study

2008 ◽  
Vol 33 (1) ◽  
pp. 35-37 ◽  
Author(s):  
Esber Çaglar ◽  
Sule Kavaloglu Cildir ◽  
Nuket Sandalli
Keyword(s):  
Potential Effect ◽  
Dental Erosion ◽  
Variable Degree ◽  
Water Control ◽  
In Situ Study ◽  
Significant Difference ◽  
Natural Spring ◽  
Erosive Effect

Objectives: Whereas the potential effect of acidic drinks in the etiology of dental erosion is well recognized the role of malt drinks is unclear. The primary aim of the present study was to compare the in vitro erosive effect on enamel produced by different aromated malt drinks. A secondary objective was to compare their erosive effects in situ with those determined in vitro. Materials and methods: To select the malt drink for the study in situ, six commercially available malt drinks were examined for erosive potential in vitro. The study in situ was a single centre, 2-period, 2-treatment crossover study to compare the erosive effect of a commercially available malt drink (Test), with that of natural spring water (Control), over 10 day periods on 10 healthy volunteers. Subjects wore upper removable appliances containing two human enamel specimens from 9 a.m. to 4 p.m. The regimen of intake of the drinks was 250 ml at midday. Measurements of enamel loss were made on samples after 5 and 10 days by profilometry. Results: The in situ study showed a statistically significant difference in erosive potential between the test and control beverages. No specimen exposed to the control beverage displayed appreciable erosion. Erosion occurred with the test drink, but to a variable degree between subjects. Conclusions: Malt drinks should be considered as potentially erosive as the results for enamel specimens exposed to the test beverage in the clinical study showed a degree of erosion that varied greatly between different participants. It is likely that under these conditions an increase in the degree of erosion would be observed in children and young people who consume malt drinks.

Hard-tissue Substrates for Evaluation of Cariogenic and Anti-cariogenic Activity in situ

1992 ◽  
Vol 71 (3_suppl) ◽  
pp. 913-919 ◽  
Author(s):  
J.R. Mellberg
Keyword(s):  
Root Surface ◽  
Hard Tissue ◽  
Suitable Alternative ◽  
Human Enamel ◽  
Human Dentin ◽  
Bovine Enamel ◽  
Root Surface Caries

Hard-tissue substrates include primarily human and bovine enamel and human dentin. They have been used for in situ studies in a natural or sound condition, as well as flattened or containing an in vitro-formed caries-like lesion. Human enamel and dentin are generally the substrates of choice for studies of coronal and root-surface caries, respectively, but bovine enamel appears to offer a suitable alternative for many studies of enamel caries. Substrates with caries-like lesions will respond more rapidly to changes in the intra-oral mineral equilibrium and will allow both demineralization and remineralization to be determined. Findings from some studies suggest that caries-like lesions may respond somewhat differently, depending upon the degree of mineralization of the surface layer. Because in vitro findings with dentin show it to be significantly more soluble in acid than enamel, results from a study that used dentin may not be directly applicable to enamel. Both enamel and dentin substrates can be used in thin-section models. Hard-tissue substrates can also differ, depending upon their intra-oral location. Locations that result in the accumulation of plaque will behave differently from those that are plaque-free. So that plaque would accumulate, substrates have been placed approximally, beneath a fabric or steel mesh, in a protected trough, beneath a metal band or within a depression on the buccal surface. For studies requiring a determination of both demineralization and remineralization, human enamel or dentin containing a surface-softened caries-like lesion and covered with a uniform natural plaque are the substrates of choice.

scholarly journals Fluoride Release from Fluoride Varnishes under Acidic Conditions

2014 ◽  
Vol 39 (1) ◽  
pp. 35-39 ◽  
Author(s):  
F Lippert
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Fluoride Varnish ◽  
Laboratory Research ◽  
Fluoride Release ◽  
Acidic Solutions ◽  
Acid Ph ◽  
Opposite Behavior ◽  
Acidic Conditions

Objective: The aim was to investigate the in vitro fluoride release from fluoride varnishes under acidic conditions. Study design: Poly(methyl methacrylate) blocks (Perspex, n=3 per group) were painted with 80±5 mg fluoride varnish (n=10) and placed into artificial saliva for 30min. Then, blocks were placed into either 1% citric acid (pH 2.27) or 0.3% citric acid (pH 3.75) solutions (n=3 per solution and varnish) for 30min with the solutions being replaced every 5min. Saliva and acid solutions were analyzed for fluoride content. Data were analyzed using three-way ANOVA (varnish, solution, time). Results: The three-way interaction was significant (p>0.0001). Fluoride release and release patterns varied considerably between varnishes. Fluoride release in saliva varied by a factor of more than 10 between varnishes. Some varnishes (CavityShield, Nupro, ProFluorid, Vanish) showed higher fluoride release in saliva than during the first 5min of acid exposure, whereas other varnishes (Acclean, Enamel-Pro, MI Varnish, Vella) showed the opposite behavior. There was little difference between acidic solutions. Conclusions: Fluoride release from fluoride varnishes varies considerably and also depends on the dissolution medium. Bearing in mind the limitations of laboratory research, the consumption of acidic drinks after fluoride varnish application should be avoided to optimize the benefit/risk ratio.

scholarly journals Effect of Hydrogen and Carbamide Peroxide in Bleaching, Enamel Morphology, and Mineral Composition: In vitro Study

2017 ◽  
Vol 18 (7) ◽  
pp. 576-582 ◽  
Author(s):  
Carmen Llena ◽  
Leopoldo Forner ◽  
Irene Esteve
Keyword(s):  
Mineral Composition ◽  
Laser Scanning ◽  
Morphological Changes ◽  
Treatment Protocol ◽  
Environmental Scanning Electron Microscopy ◽  
In Vitro Study ◽  
Confocal Laser ◽  
Carbamide Peroxide ◽  
Tooth Color

ABSTRACT Aim The aim of the study was to evaluate the bleaching effect, morphological changes, and variations in calcium (Ca) and phosphate (P) in the enamel with hydrogen peroxide (HP) and carbamide peroxide (CP) after the use of different application regimens. Materials and methods Four groups of five teeth were randomly assigned, according to the treatment protocol: HP 37.5% applied for 30 or 60 minutes (HP30, HP60), CP 16% applied for 14 or 28 hours (CP14, CP28). Changes in dental color were evaluated, according to the following formula: ΔE = [(La−Lb)2+(aa−ab)2 + (ba−bb)2]½. Enamel morphology and Ca and P compositions were evaluated by confocal laser scanning microscope and environmental scanning electron microscopy. Results ΔE HP30 was significantly greater than CP14 (10.37 ± 2.65/8.56 ± 1.40), but not between HP60 and CP28. HP60 shows greater morphological changes than HP30. No morphological changes were observed in the groups treated with CP. The reduction in Ca and P was significantly greater in HP60 than in CP28 (p < 0.05). Conclusion Both formulations improved tooth color; HP produced morphological changes and Ca and P a gradual decrease, while CP produced no morphological changes, and the decrease in mineral component was smaller. Clinical significance CP 16% applied during 2 weeks could be equally effective and safer for tooth whitening than to administer two treatment sessions with HP 37.5%. How to cite this article Llena C, Esteve I, Forner L. Effect of Hydrogen and Carbamide Peroxide in Bleaching, Enamel Morphology, and Mineral Composition: In vitro Study. J Contemp Dent Pract 2017;18(7):576-582.

scholarly journals Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study

2021 ◽  
Vol 35 ◽  
Author(s):  
Maria Denise Rodrigues DE MORAES ◽  
Vanara Florêncio PASSOS ◽  
Gislaine Cristina PADOVANI ◽  
Lady Clarissa Brito da Rocha BEZERRA ◽  
Ilka Maria VASCONCELOS ◽  
...  
Keyword(s):  
Protective Effect ◽  
Green Tea ◽  
Tea Catechins ◽  
In Situ Study ◽  
Green Tea Catechins ◽  
Human Dentin

scholarly journals The In Situ Effect of Titanium Tetrafluoride Gel on Erosion/Abrasion Progression in Human Dentin

2017 ◽  
Vol 28 (3) ◽  
pp. 337-345
Author(s):  
Taís Fonseca Mantilla ◽  
Cecilia Pedroso Turssi ◽  
Thayanne Monteiro Ramos-Oliveira ◽  
Camila Vieira da Silva ◽  
Luis Cláudio Suzuki ◽  
...  
Keyword(s):  
Citric Acid ◽  
Repeated Measures ◽  
Environmental Scanning Electron Microscopy ◽  
Control Groups ◽  
Titanium Tetrafluoride ◽  
Surface Loss ◽  
Significant Difference ◽  
Human Dentin

Abstract Erosion incidence is increasing and its control is still a challenge in clinical practice. This study evaluated 4% TiF4-gel effects on eroded human dentin subjected to in situ erosive/abrasive episodes. Seventy-two previously eroded dentin slabs (0.05 M citric acid, pH 2.3, 20 min) were allocated to 6 groups (n=12) according to the treatment to be performed during the in situ phase and number of erosive/abrasive cycles, as follows: 4% TiF4-gel applied once (TiF41), twice (TiF42) or three times (TiF43) followed by 1, 2 and 3 erosive/abrasive cycles, respectively. Gel was applied before the beginning of the next cycle. Control groups were subjected to 1 (C1), 2 (C2) and 3 (C3) erosive/abrasive cycles only. A seventh group (n=12) comprised in vitro uneroded samples (UN) subjected to 3 erosive/abrasive cycles. Each cycle corresponded to 2 days of erosive (citric acid 0.5%, pH 2.6, 6x/day) and abrasive (electric toothbrush, 10 s/sample, 1 x/day) challenges. Samples were evaluated under profilometry and environmental scanning electron microscopy (ESEM). Atomic force microscopy images (AFM) were also made (n=3). Repeated measures 2-way ANOVA and Tukey test (p<0.001) showed that TiF42, which did not differ from TiF41 and TiF43, revealed a significant reduction in surface loss compared to all control groups. TiF41 and TiF43 showed no significant difference from C1, but both groups demonstrated significantly smaller surface loss than C2 and C3. ESEM and AFM micrographs suggested alterations on treated surfaces compared to samples from control groups, showing reduced diameters of dentinal tubules lumens. Therefore, TiF4 was able to reduce the progression of erosive/abrasive lesions.

scholarly journals Effect of starch on the cariogenic potential of sucrose

2005 ◽  
Vol 94 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Cecilia C. C. Ribeiro ◽  
Cínthia P. M. Tabchoury ◽  
Altair A. Del Bel Cury ◽  
Livia M. A. Tenuta ◽  
Pedro L. Rosalen ◽  
...  
Keyword(s):  
Animal Studies ◽  
Amylase Activity ◽  
Negative Control ◽  
Cross Sectional ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Two Phases ◽  
Mineral Loss

Sincein vitroand animal studies suggest that the combination of starch with sucrose may be more cariogenic than sucrose alone, the study assessedin situthe effects of this association appliedin vitroon the acidogenicity, biochemical and microbiological composition of dental biofilm, as well as on enamel demineralization. During two phases of 14 d each, fifteen volunteers wore palatal appliances containing blocks of human deciduous enamel, which were extra-orally submitted to four groups of treatments: water (negative control, T1); 2 % starch (T2); 10 % sucrose (T3); and 2 % starch+10 % sucrose (T4). The solutions were dripped onto the blocks eight times per day. The biofilm formed on the blocks was analysed with regard to amylase activity, acidogenicity, and biochemical and microbiological composition. Demineralization was determined on enamel by cross-sectional microhardness. The greatest mineral loss was observed for the association starch+sucrose (P<0·05). Also, this association resulted in the highest lactobacillus count in the biofilm formed (P<0·05). In conclusion, the findings suggest that a small amount of added starch increases the cariogenic potential of sucrose.

Sign in / Sign up

Export Citation Format

Share Document