scholarly journals Profile of Fluoride Release from a Nanohybrid Composite Resin

2015 ◽  
Vol 3 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Raquel Assed Bezerra Silva ◽  
Fernanda Regina Ribeiro Santos ◽  
Augusto Cesar Cropanese Spadaro ◽  
Ana Cristina Morseli Polizello ◽  
Andiara De Rossi ◽  
...  
Keyword(s):  
Composite Resin ◽  
Artificial Saliva ◽  
Glass Ionomer Cement ◽  
Positive Control ◽  
Negative Control ◽  
Fluoride Release ◽  
Fluoride Ions ◽  
Significance Level ◽  
Nanohybrid Composite

The aim of this study was to evaluate in vitro the amount and profile of fluoride release from a fluoride-containing nanohybrid composite resin (Tetric® N-Ceram) by direct potentiometry. Thirty specimens (5 mm diameter x 3 mm high; n=10/material) were made of Tetric® N-Ceram, Vitremer® resin-modified glass ionomer cement (RMGIC) (positive control) or Filtek® Z350 nanofill composite resin (negative control). The specimens were stored individually in plastic tubes containing 1 mL of artificial saliva at 37°C, which was daily renewed during 15 days. At each renewal of saliva, the amount of fluoride ions released in the solution was measured using a fluoride ion-selective electrode with ion analyzer, and the values obtained in mV were converted to ppm (µg/mL). Data were analyzed statistically by ANOVA and Tukey’s post-hoc test at a significance level of 5%. The results showed that the resins Tetric® N-Ceram and Filtek® Z350 did not release significant amounts of fluoride during the whole period of evaluation (p>0.05). Only Vitremer® released significant amounts of fluoride ions during the 15 days of the experiment, with greater release in first 2 days (p<0.05) and stabilization in the subsequent days (p>0.05). In conclusion, the nanohybrid composite resin Tetric® N-Ceram did not present in vitro fluoride-releasing capacity throughout the 15 days of study.

scholarly journals Evaluation of ionic degradation and slot corrosion of metallic brackets by the action of different dentifrices

2013 ◽  
Vol 18 (1) ◽  
pp. 86-93
Author(s):  
Gustavo Antônio Martins Brandão ◽  
Rafael Menezes Simas ◽  
Leandro Moreira de Almeida ◽  
Juliana Melo da Silva ◽  
Marcelo de Castro Meneghim ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Artificial Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Sem Analysis ◽  
Wilcoxon Test ◽  
Surface Polishing ◽  
Aluminium Ion ◽  
Negative Controls

OBJECTIVE: To evaluate the in vitro ionic degradation and slot base corrosion of metallic brackets subjected to brushing with dentifrices, through analysis of chemical composition by Energy Dispersive Spectroscopy (EDS) and qualitative analysis by Scanning Electron Microscopy (SEM). METHODS: Thirty eight brackets were selected and randomly divided into four experimental groups (n = 7). Two groups (n = 5) worked as positive and negative controls. Simulated orthodontic braces were assembled using 0.019 x 0.025-in stainless steel wires and elastomeric rings. The groups were divided according to surface treatment: G1 (Máxima Proteção Anticáries®); G2 (Total 12®); G3 (Sensitive®); G4 (Branqueador®); Positive control (artificial saliva) and Negative control (no treatment). Twenty eight brushing cycles were performed and evaluations were made before (T0) and after (T1) experiment. RESULTS: The Wilcoxon test showed no difference in ionic concentrations of titanium (Ti), chromium (Cr), iron (Fe) and nickel (Ni) between groups. G2 presented significant reduction (p < 0.05) in the concentration of aluminium ion (Al). Groups G3 and G4 presented significant increase (p < 0.05) in the concentration of aluminium ion. The SEM analysis showed increased characteristics indicative of corrosion on groups G2, G3 and G4. CONCLUSION: The EDS analysis revealed that control groups and G1 did not suffer alterations on the chemical composition. G2 presented degradation in the amount of Al ion. G3 and G4 suffered increase in the concentration of Al. The immersion in artificial saliva and the dentifrice Máxima Proteção Anticáries® did not alter the surface polishing. The dentifrices Total 12®, Sensitive® and Branqueador® altered the surface polishing.

scholarly journals Microbiological Sealing Analysis of a Tapered Connection and External Hexagon System

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Gardel Nepomuceno Costa ◽  
Elizabeth Ferreira Martinez ◽  
Aluísio Martins de Oliveira Ruellas ◽  
Daiane Cristina Peruzzo ◽  
Júlio Cesar Joly ◽  
...  
Keyword(s):  
Descriptive Analysis ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Microbiological Analysis ◽  
Significance Level ◽  
Implant Abutment ◽  
Bacterial Leakage ◽  
Tapered Connection

Considering the variety of implant connection systems available in the market and the contrasting literature regarding tapered connection systems in terms of bacterial leakage, the aim of this in vitro study was to compare the effectiveness of the bacterial seal at the implant/abutment interface between an external hexagon and a tapered connection system. Twelve sets of indexed tapered connection components and twelve sets of external hexagon connection components were used for microbiological analysis. In addition, for each model, an implant with its respective prosthetic abutment was used as a negative control and another as a positive control of microbial contamination. Failure of the abutment/implant interface seal was observed via turbidity or presence of deposits in the culture. Descriptive analysis of the data and relative frequency (percentage) as well as Fisher’s exact test were used at a significance level of 5%. Two of ten (20%) external hexagon specimens showed contamination against 0/10 (0%) tapered connection implants. In conclusion, both implant/abutment connections were able to prevent bacterial leakage in vitro.

scholarly journals Effect of putri malu (Mimosa pudica) extract on Ascaris suum mortality in vitro

2011 ◽  
Vol 9 (2) ◽  
pp. 33-37
Author(s):  
MUHAMMAD ARIF NUR SYAHID ◽  
CR. SITI UTARI ◽  
SUTARMIADJI DJUMARGA
Keyword(s):  
Ascaris Suum ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mimosa Pudica ◽  
Control Group Design ◽  
Significance Level ◽  
Death Time ◽  
Significant Difference

Syahid MAN, Utari CRS, Djumarga S. 2011. Effect of putri malu extract (Mimosa pudica) on Ascaris suum mortality in vitro. Biofarmasi 9: 33-37. This study was to determine the influence of Mimosa pudica extract in Ascaris suum mortality. This research was a laboratory experiment, with a post-test only with control group design by using 140 adult A. suum, divided into seven groups. This research used NaCl 0.9% for a negative control, pirantel pamoat 5 mg/mL solution for a positive control, and five intervention by using 20%, 40%, 60%, 80% and 100% concentration of M. pudica extract. The observation was conducted in every two hours until worm death and it was started to be counted after all worm death. Data were analyzed with one-way ANOVA test continued with Least Significance Difference (LSD) by using SPP for Window Release 17 with a significance level p<0.05. The results showed that all A. suum death in 96 hours at negative control, 2 hours at positive control, 29.5 hours at 20% M. pudica extract, 24.5 hours at 40% M. pudica extract, 16 hours at 60% M. pudica extract, 12 hours at 80% M. pudica extract and 4 hours at 100% M. pudica extract. There was a significant difference in the death time of A. suum in all research groups. From the result of research, it could be concluded that the extract of putri malu had an effect on accelerating A. suum mortality time.

scholarly journals In vitro study of dentin hypersensitivity treated by whitlockite glass-ceramics

2017 ◽  
Author(s):  
Amanda C. Juraski
Keyword(s):  
Bioactive Glass ◽  
Artificial Saliva ◽  
Third Molar ◽  
Glass Ceramics ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Dentin Hypersensitivity ◽  
Human Teeth

AbstractThe aim of this study is to evaluate the potential of a bioactive glass based on the 3CaO.P2O5-SiO2MgO-system and its glassceramics containing whitlockite on the remineralization of dentin as a possible treatment to dentin hypersensitivity. For that, 40 third molar human teeth were artificially demineralized and randomly distributed in 4 groups (n = 10): G1 - Negative Control (no treatment), G2 - Positive Control (treated by Bioglass® 45S5), G3 – BG (treated by bioactive glass based on 3CaO.P2O5-SiO2-MgOsystem), and G4 – BGC (treated by bioactive whitlockite glass-ceramics). After treatment, the samples were emerged in artificial saliva and stored for 7 days in a controlled temperature of 37ºC. After that, scanning electron microscopy (SEM) and atomic force microscopy (AFM) was used to evaluate samples morphology. The analysis confirmed the formation of hydroxyapatite on the surface of all the biomaterials studied, that in the dentine specimens treated by bioactive glass and whitlockite glas-ceramic most of the dentinal tubules were completely occluded.

scholarly journals In vitro efficacy of Carapa guianensis Aublet (Meliaceae) oil on Damalinia (Bovicola) ovis Schrank (1781)

2021 ◽  
Vol 15 (4) ◽  
pp. 330-334
Author(s):  
Antônia Aniellen Raianne Moises Aguiar ◽  
Caio Michel de Morais Rolim ◽  
Iany Leda Moreira Dantas ◽  
Weibson Pinheiro Paz André ◽  
Wesley Adson Costa Coelho ◽  
...  
Keyword(s):  
Small Ruminants ◽  
Tween 80 ◽  
Positive Control ◽  
Negative Control ◽  
Probit Regression ◽  
Significance Level ◽  
Carapa Guianensis ◽  
Insecticidal Effect ◽  
Insecticidal Action

Productivity in small ruminants can be compromised due to lice infestations. This pest infestation is controlled by the administration of insecticides, and their excessive use causes resistance. New control alternatives are being investiga-ted, especially the use of phytotherapeutics. Research using the oil from Carapa guianensis (andiroba) has shown insecticidal action on feline and goat lice specimens. The present study aimed to evaluate the efficacy of C. guianensis seed oil, in vitro, on Damalinia (Bovicola) ovis.Nine hundred specimens of D. (Bovicola) ovis were randomly assigned into groups and were subjected to different treatments: (G1) 1,000 to 15.6 mg/mL of C. guianensis oil; (G2) 3% Tween 80 (negative control); and (G3) amitraz 1.25 g/mL (positive control). Data presentation was expressed as mean efficacy ± standard deviation using the statistical program SPSS version 23.0 and statistical differences of the experimental groups were obtained by Kruskal-Wallis and Friedman. Probit Regression was performed to obtain CI50 and CI90, with a significance level of p < 0.05. The oil with a concentration of 1,000 mg/mL had an efficacy of 94% after 24 hours of exposure, differing statistically from the positive and negative control (p < 0.05). The IC50 and IC90 values were 439.21 mg/mL and 807.09 mg/mL, respectively. The action of the oil on the specimens was independent whether it was an adult female, male, or nymph. It is concluded that C. guianensis oil showed in vitro insecticidal effect against D. (Bovicola) ovis.

scholarly journals Antimicrobial Effects of Dental Luting Glass Ionomer Cements onStreptococcus mutans

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sina Klai ◽  
Markus Altenburger ◽  
Bettina Spitzmüller ◽  
Annette Anderson ◽  
Elmar Hellwig ◽  
...  
Keyword(s):  
Glass Ionomer Cement ◽  
Human Saliva ◽  
Negative Control ◽  
Glass Ionomer ◽  
Fluoride Ions ◽  
Luting Cements ◽  
Antimicrobial Effects ◽  
Antimicrobial Potential

Objective. To reduce secondary caries, glass ionomer luting cements are often used for cementing of indirect restorations. This is because of their well-known antimicrobial potential through the release of fluoride ions. The aim of thisin vitrostudy was to investigate the antimicrobial effect of five dental luting cements which were based on glass ionomer cement technology.Methods. Five different glass ionomer based luting cements were tested for their antimicrobial effects onStreptococcus mutansin two different experimental setups: (i) determination of colony-forming units (CFUs) in a plate-counting assay; (ii) live/dead staining (LDS) and fluorescence microscopy. All experiments were conducted with or without prior treatment of the materials using sterilized human saliva. Antimicrobial effects were evaluated for adherent and planktonic bacteria. Bovine enamel slabs (BES) were used as negative control. BES covered with 0.2% chlorhexidine (CHX) served as positive control.Results. Each of the tested materials significantly reduced the number of initially adhered CFUs; this reduction was even more pronounced after prior incubation in saliva. Antimicrobial effects on adherent bacteria were confirmed by live-dead staining.Conclusion. All five luting cements showed an antimicrobial potential which was increased by prior incubation with human saliva, suggesting an enhanced effectin vivo.

scholarly journals Fluoride Release from Restorative Materials

2011 ◽  
Vol 22 (5) ◽  
pp. 355-358 ◽  
Author(s):  
Gabriela Romanini Basso ◽  
Álvaro Della Bona ◽  
Delton Luiz Gobbi ◽  
Dileta Cecchetti
Keyword(s):  
Standard Deviation ◽  
Liquid Membrane ◽  
Glass Ionomer Cement ◽  
Negative Control ◽  
The Other ◽  
Fluoride Release ◽  
Glass Ionomer ◽  
Restorative Materials ◽  
Ionomer Cement

The aim of this study was to evaluate in vitro fluoride (F) release from 4 restorative materials (3M ESPE): Ketak Molar Easymix [KME - conventional glass ionomer cement (GIC)]; Rely-X luting 2 [RL2 - resin-modified GIC (RMGIC)]; Vitremer (VIT- RMGIC); and Filtek Z250 [Z250 - negative control]. Disc-shaped specimens were fabricated according to the manufacturer’s instructions and placed into 10 mL of reverse osmosis water at 37°C until the analyses were done using a liquid membrane for selective F ion electrode (Orion 710). F release was evaluated every 6 h in the first day and thereafter daily during 28 days (d). The results were analyzed statistically by two-way ANOVA and Tukey’s test (α=0.05). Mean F release and standard deviation values (in ppm) were: KME: 6 h- 0.34 ± 0.04; 24 h- 1.22 ± 0.30; 7 d- 0.29 ± 0.09; 14 d- 0.20 ± 0.04; 28 d- 0.16 ± 0.01; RL2: 6 h- 2.46 ± 0.48; 24 h-12.33 ± 2.93; 7 d- 1.37 ± 0.38; 14 d- 0.80 ± 0.13; 28 d- 0.80 ± 0.21; VIT: 6 h- 0.98 ± 0.35; 24 h- 4.35 ± 1.22; 7 d- 0.66 ± 0.23; 14 d- 0.40 ± 0.07; 28 d- 0.39 ± 0.08; Z250: 6 h- 0.029 ± 0.001; 24 h- 0.024 ± 0.009; 7 d- 0.023 ± 0.004; 14 d- 0.025 ± 0.001; 28 d- 0.028 ± 0.001. RL2 RMGIC released more F than the other materials in all periods. The greatest release of F occurred in the first 24 h.

scholarly journals Comparative Effects of CPP-ACP and Xylitol F-Varnishes on the Reduction of Tooth Erosion and Its Progression

2020 ◽  
Vol 31 (6) ◽  
pp. 664-672
Author(s):  
Adílis Alexandria ◽  
Ana Maria Gondim Valença ◽  
Lúcio Mendes Cabral ◽  
Lucianne Cople Maia
Keyword(s):  
Artificial Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Deionized Water ◽  
3D Images ◽  
Significance Level ◽  
Tooth Structure ◽  
Varnish Layer ◽  
One Way Anova ◽  
Better Than

Abstract Evaluated the effect of CPP-ACP/NaF and xylitol/NaF varnishes in reduce erosion and progression of erosion. Forty enamel blocks were divided into four groups (n=10): G1=CPP-ACP/NaF varnish (MI varnishTM); G2=xylitol/NaF varnish (Profluorid®); G3=NaF varnish (Duraphat®, positive control) and G4=deionized water (MilliQ®, negative control). Samples were immersed in Sprite ZeroTM (pH 2.58, 4x/day, 3 days), in between immersions, the specimens stayed in artificial saliva. After 3 days of erosion, the eroded area was divided in two (half of one received an additional varnish layer while the other half repeated the same 3-day erosion cycle). The 3D, non-contact profilometry technique was used to determinate tooth structure loss (TSL) and surface roughness (SR). Scanning electron microscopy (SEM) and 3D images were utilized to evaluate the topography of the samples. Mann-Whitney, one-way ANOVA and Tukey tests were used (significance level of 0.05%). SEM and 3D images were descriptively evaluated. After 3 or 6 days of erosion, all tested varnishes were better than G4 (p<0.05) for TSL and SR. In addition, G1 had lower values for TSL than G3 (p<0.05) after 3 days of erosion. Under SEM and 3D images observation, all groups presented porosity, irregularities and depressions on the surface enamel after 3 and 6 days of erosion, more pronounced in G4. An application of topical NaF varnishes was effective in reducing TSL and enamel roughness after erosion challenges, being the CCP-ACP/NaF varnish more effective than NaF varnish and water after 3 days of erosion.

scholarly journals Effect of a CO2Laser on the Inhibition of Root Surface Caries Adjacent to Restorations of Glass Ionomer Cement or Composite Resin: AnIn VitroStudy

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
L. C. Daniel ◽  
F. C. Araújo ◽  
B. R. Zancopé ◽  
F. S. Hanashiro ◽  
M. Nobre-dos-Santos ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Composite Resin ◽  
Glass Ionomer Cement ◽  
Root Surface ◽  
Positive Control ◽  
Negative Control ◽  
Glass Ionomer ◽  
Cavity Preparation ◽  
Mineral Loss ◽  
Ionomer Cement

This study investigated the effect of CO2laser irradiation on the inhibition of secondary caries on root surfaces adjacent to glass ionomer cement (GIC) or composite resin (CR) restorations. 40 dental blocks were divided into 4 groups: G1 (negative control): cavity preparation + adhesive restoration with CR; G2: (positive control) cavity preparation + GIC restoration; G3: equal to group 1 + CO2laser with 6 J/cm2; G4: equal to group 2 + CO2laser. The blocks were submitted to thermal and pH cycling. Dental demineralization around restorations was quantified using microhardness analyses and Light-Induced Fluorescence (QLF). The groups showed no significant differences in mineral loss at depths between 20 μm and 40 μm. At 60 μm, G2 and G3 ≠ G1, but G4 = G1, G2 and G3. At 80 μm, G4 ≠ G1, and at 100 μm, G4 = G2 = G1. At 140 and 220 μm, G2, G3, and G4 = G1. The averages obtained using QFL in groups 1, 2, 3, and 4 were 0.637, 0.162, 0.095, and 0.048, respectively. QLF and microhardness analyses showed that CO2laser irradiation reduced mineral loss around the CR restorations but that it did not increase the anticariogenic effect of GIC restorations.

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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