scholarly journals CLINICO-LABORATORY INDICES CHARACTERIZING THE IMMUNE SYSTEM OF CHICKENPOX PATIENTS

2017 ◽  
Vol 22 (4) ◽  
pp. 190-194
Author(s):  
Yu. S Kalinina ◽  
A. A Savchenko ◽  
I. V Kudryavtsev ◽  
Elena P. Tikhonova ◽  
A. G Borisov
Keyword(s):  
Flow Cytometry ◽  
Innate Immunity ◽  
Immune System ◽  
T Helper Cells ◽  
Laboratory Data ◽  
Relative Number ◽  
Absolute Number ◽  
T Helper ◽  
Chicken Pox ◽  
Declining Populations

The aim of the study was the evaluation of the function of the immune system in chicken pox patients based on clinical and laboratory data. There were examined 75 patients. The study on phenotypic composition of cells in innate immunity was executed with the method of flow cytometry. A detailed study of the immune system in chickenpox patients revealed the alteration in the composition of the subpopulation of T-lymphocytes, with declining populations of T-helper cells, namely, the lowering of relative and absolute number of CD3+ CD4+ and an increase in the relative number of CD3+CD8+ lymphocytes, which is indicative of immunosuppression. No changes according to the number of NK cells, which must actively respond to viral infection (the final stage of activation of cell growth with an increase in their number in peripheral blood). The absence of their increase is indicative of the irresponsive immunity in chickenpox patients

scholarly journals Neonatal lymphocyte subpopulations analysis and maternal preterm premature rupture of membranes: a pilot study

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Margherita Amadi ◽  
Silvia Visentin ◽  
Francesca Tosato ◽  
Paola Fogar ◽  
Giulia Giacomini ◽  
...  
Keyword(s):  
T Cells ◽  
Immune System ◽  
T Cell ◽  
T Helper Cells ◽  
Lymphocyte Subpopulations ◽  
T Helper ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Preterm Premature Rupture ◽  
Helper Cells

Abstract Objectives Preterm premature rupture of membranes (pPROM) causes preterm delivery, and increases maternal T-cell response against the fetus. Fetal inflammatory response prompts maturation of the newborn’s immunocompetent cells, and could be associated with unfavorable neonatal outcome. The aims were to examine the effects of pPROM (Mercer BM. Preterm premature rupture of the membranes: current approaches to evaluation and management. Obstet Gynecol Clin N Am 2005;32:411) on the newborn’s and mother’s immune system and (Test G, Levy A, Wiznitzer A, Mazor M, Holcberg G, Zlotnik A, et al. Factors affecting the latency period in patients with preterm premature rupture of membranes (pPROM). Arch Gynecol Obstet 2011;283:707–10) to assess the predictive value of immune system changes in neonatal morbidity. Methods Mother-newborn pairs (18 mothers and 23 newborns) who experienced pPROM and controls (11 mothers and 14 newborns), were enrolled. Maternal and neonatal whole blood samples underwent flow cytometry to measure lymphocyte subpopulations. Results pPROM-newborns had fewer naïve CD4 T-cells, and more memory CD4 T-cells than control newborns. The effect was the same for increasing pPROM latency times before delivery. Gestational age and birth weight influenced maturation of the newborns’ lymphocyte subpopulations and white blood cells, notably cytotoxic T-cells, regulatory T-cells, T-helper cells (absolute count), and CD4/CD8 ratio. Among morbidities, fewer naïve CD8 T-cells were found in bronchopulmonary dysplasia (BPD) (p=0.0009), and more T-helper cells in early onset sepsis (p=0.04). Conclusions pPROM prompts maturation of the newborn’s T-cell immune system secondary to antigenic stimulation, which correlates with pPROM latency. Maternal immunity to inflammatory conditions is associated with a decrease in non-major histocompatibility complex (MHC)-restricted cytotoxic cells.

Antigen-Dependent Cytokine mRNA Expression by Individual Rhesus Macaque T Helper Cells by Flow Cytometry

2000 ◽  
Vol 201 (2) ◽  
pp. 94-108 ◽  
Author(s):  
Juan Arredondo ◽  
F. Xusheng Lü ◽  
Francois Villinger ◽  
Michael B. McChesney ◽  
Jerry R. McGhee ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Mrna Expression ◽  
Rhesus Macaque ◽  
T Helper Cells ◽  
T Helper ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression ◽  
Helper Cells

scholarly journals Analysis of the cellular immunity status in patients with pathological placentation

2021 ◽  
Vol 8 (3) ◽  
pp. 167-172
Author(s):  
Edvard A. Berg ◽  
Alfiya G. Yashchuk ◽  
Il’nur I. Musin ◽  
Raisa A. Naftulovich ◽  
Elena M. Popova
Keyword(s):  
Cellular Immunity ◽  
Relative Number ◽  
Absolute Number ◽  
Relative Increase ◽  
Placental Pathology ◽  
T Helper ◽  
Cell Immunity ◽  
History Of ◽  
A Prospective Study ◽  
Cluster Of Differentiation

AIM: The study aimed to investigate the cellular immunity in patients with placental disposition. MATERIALS AND METHODS: A prospective study analyzed birth histories and clinical and laboratory parameters of 10 patients with placental disposition. The cellular immunity status was determined by analyzing lymphocytes with a cluster of differentiation (CD), including CD3+, CD4+, CD8+, CD16+56, CD3-СD8+, TNK, and CD38+8+. Obtained data were analyzed statistically. RESULTS: Patients were 32.0 (29.0; 36.0) years old. As regards reproductive history, 60.0% had a history of three pregnancies, 20% had two pregnancies, 10% had their first pregnancy, and 10% had their fourth pregnancy. Moreover, placenta dispositions most often occurred at the second pregnancy in 70.0%, at the third pregnancy in 20.0%, and first pregnancy in 10%. In terms of cell immunity in comparison with normal indicators, the relative number of natural killer cells (CD16+56+), including activated CD3-СD8+, tended to increase. A relative increase in cytotoxic T-lymphocytes (СD8+) was found against the background of lower number of T-helper cells, along with general immunodeficiency (immunoregulatory index in the absolute number of women was less than 1.5). CONCLUSIONS: Further investigation of cellular immunity in women with placental pathology is relevant to detect additional pathogenetic mechanisms of the development of obstetric complications.

Immunological Testing in Assisted Reproductive Technology

2021 ◽  
Author(s):  
Joshua Odendaal ◽  
Siobhan Quenby
Keyword(s):  
Immune System ◽  
Assisted Reproductive Technology ◽  
T Helper Cells ◽  
Reproductive Technology ◽  
Implantation Failure ◽  
T Helper ◽  
Recurrent Implantation Failure ◽  
New Era ◽  
Uterine Natural Killer Cells ◽  
Maternal Immune System

AbstractFetal implantation requires carefully orchestrated involvement of the maternal immune system. Aberrant function within implantation has been suggested as a cause of implantation failure. The emergence of immunological theories of miscarriage has led to immunological testing as an adjuvant treatment in assisted reproductive technology; however, it remains controversial, with mixed evidence both for immunological cause and the benefits of immunological testing. Literature on common methods of immunological testing within assisted reproductive technology is reviewed including those of peripheral and uterine natural killer cells, chronic endometritis, and T-helper cells cytokine ratio. There is little consensus in the evidence on immunological testing in the context of recurrent implantation failure. The field is limited by a lack of uniformity in approach to testing and heterogeneity of the pathophysiological cause. Nevertheless, the maternal immune system is heavily involved in implantation and the new era of personalized medicine ensures that a more defined approach to immunological testing will be achieved.

Molecular mechanisms for B lymphocyte selection: induction and regulation of antigen-receptor-mediated apoptosis of mature B cells in normal mice and their defect in autoimmunity-prone mice

1994 ◽  
Vol 345 (1313) ◽  
pp. 297-301 ◽  
Keyword(s):  
Immune System ◽  
B Cells ◽  
B Cell ◽  
T Helper Cells ◽  
Molecular Mechanisms ◽  
B Lymphocyte ◽  
Antigen Receptor ◽  
Normal Function ◽  
T Helper ◽  
Helper Cells

Apoptosis (programmed cell death) has been suggested to be involved in clonal elimination of selfreactive lymphocytes for the normal function of the immune system. By crosslinking the antigen receptor (surface immunoglobulin; slg) on the peritoneal B cells of normal mice, we found that strong crosslinking of slg induces apoptosis of mature B cells, suggesting that interaction with membranebound self-antigens may eliminate self-reactive mature B cells by apoptosis. Antigen-receptor-mediated B cell apoptosis is blocked when a signal is transduced via the CD40 molecule on the B cell surface. Because the ligand of CD40 (CD40L) is expressed on activated T helper cells, B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens, which are normally able to activate T helper cells. Moreover, slg crosslinking fails to induce apoptosis of both bcl-2-transgenic mice and autoimmune-disease-prone New Zealand mice. In these mice, the defect in slg-mediated apoptosis of mature B cells may allow generation of self-reactive B cells, resulting in pathogenic consequences.

scholarly journals ВПЛИВ ІМУНОМОДУЛЯТОРІВ НА ПОКАЗНИКИ КЛІТИННОГО ІМУНІТЕТУ ЛОШАТ ВЕРХОВИХ ПОРІД

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 45-49
Author(s):  
I.P. Кrytsia
Keyword(s):  
T Cells ◽  
Immune System ◽  
T Lymphocytes ◽  
Functional Activity ◽  
T Helper Cells ◽  
Hematological Parameters ◽  
Immune Reactivity ◽  
T Helper ◽  
Physiological Level ◽  
Helper Cells

To maintain a body at sufficient physiological level the effective functioning of the immune system, which determines the resistance and immune reactivity of animals, is necessary. In our studies in newborn foals indicators of cellular immunity were mature. During the studying of foals of all ages were established the reduction of hematological parameters in animals months of age.The use of immunomodulators prevents the immunodeficiency in animals. Immunomodulators introduction for animals normalizes T–immune system, in particular, increases the number of leucocytes in the blood, lymphocytes of certain populations, especially teofilin–resistant subpopulation of T–helper cells, increases the functional activity of lymphocytes.Under influence of ribotan revealed a trend to the increasing of T–lymphocytes by 0.2 – 1.2% (0.4 – 2.3%), respectively in Purebred Saddle and Ukrainian Saddle breeds. Results of the content of T–helper and T–suppressor cells in foals blood after ribotan administration showed that the use of immunomodulators not only increases the number of T–helper cells, but restores the ratio T–h / T–s, which returned to the optimal rate (1.9). Analyzing the functional status of T–lymphocytes during the application of immunomodulators was found the probable increase of the number of activated T–lymphocytes in Purebred Saddle foals more than 2–fold (P <0.01) and trend to increase of these cells in Ukrainian Saddle foals. In relation to thermostable T–lymphocytes, was note that the trend to the most optimal level of these cells installed in foals after administration of ribotan (values within 3 – 4%). The increasing in number of thermostable T–cells more than 4% indicates an increase power of suppressor T–cells population, indicating the inhibition of T–helper cells, and therefore the production of antibodies. Thus, the use of ribotan in dose of 1 ml / animal for three days leads to an increasing in 1.4 – 4.5% of the number of leukocytes in the blood of experimental group of foals compared with control animals. Under influence of ribotan in the blood of foals increases cell (number of T–lymphocytes in 0.4 – 2.3%) and functional activity (T–active lymphocytes in 2.3 times; P < 0.05) T–immune system. Under influence of cycloferon in the blood of foals increases the functional activity of T–immune system (the number of T–active lymphocytes in 16.7 – 25%; P < 0.05). 

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