scholarly journals Study of the antioxidant status in patients with secondary lymphedema of the lower extremities under conservative treatment

2021 ◽  
Vol 102 (3) ◽  
pp. 277-283
Author(s):  
Roman E. Kalinin ◽  
Igor A. Suchkov ◽  
Denis A. Maksaev ◽  
Yulia V. Abalenikhina
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Healthy Volunteers ◽  
Antioxidant Status ◽  
Lower Extremities ◽  
Compression Therapy ◽  
Secondary Lymphedema ◽  
Elastic Compression ◽  
Micronized Purified Flavonoid Fraction ◽  
Flavonoid Fraction

Aim. To assess the antioxidant status in patients with secondary lymphedema of the lower extremities who undergo different types of conservative treatment. Methods. The study included 90 patients with secondary lymphedema of the lower extremities and 30 healthy volunteers. Group 1 participants (n=30) received compression therapy and Vitamin E at a dose of 400 IU/day, group 2 participants (n=30) compression therapy and a micronized purified flavonoid fraction 1000 mg/day, group 3 (n=30) compression therapy alone. Group 4 (n=30) comprised healthy volunteers. The level of malondialdehyde, the activity of superoxide dismutase, glutathione peroxidase, catalase, and the level of non-protein thiols (SH-groups) were determined at inclusion in the study and then after 1 and 3 months. Results. In patients with secondary lymphedema, the initial level of glutathione peroxidase was higher by 768.22%, catalase by 420.5%, malondialdehyde by 60%, and the level of SH-groups was lower by 65,71% compared with the group of volunteers. In the first group, there was a significant decrease of 36.1% in the level of superoxide dismutase and a significant increase of 89.9% in the level of glutathione peroxidase at the end of therapy when compared with the level after 1 month. In the second group, catalase level significantly increased by 33.3%, superoxide dismutase by 17.6%, and glutathione peroxidase by 61.3% compared to baseline values. The biochemical indicators of the endothelium significantly increased when using a combination of micronized purified flavonoid fraction and elastic compression in comparison with elastic compression alone and a combination with Vitamin E. In the third group, there were no significant differences in the levels of biochemical indicators of endothelial function. Conclusion. Increased formation of lipid peroxidation products along with a decrease in the activity of antioxidant systems was revealed in patients with lower extremity secondary lymphedema compared with healthy volunteers; the most effective therapy aimed at correcting endothelial cell dysfunction is the use of micronized purified flavonoid fraction and elastic compression.

Clinical effectiveness of bioflavonoids in the treatment of secondary lower limb lymphedema

2021 ◽  
Vol 29 (2) ◽  
pp. 245-250
Author(s):  
Roman E. Kalinin ◽  
Igor A. Suchkov ◽  
Denis A. Maksaev
Keyword(s):  
Lower Limb ◽  
Adverse Reactions ◽  
Clinical Effectiveness ◽  
Lower Limbs ◽  
Compression Stockings ◽  
Secondary Lymphedema ◽  
Elastic Compression ◽  
Positive Clinical Effect ◽  
Micronized Purified Flavonoid Fraction ◽  
Flavonoid Fraction

AIM: This study aimed to investigate the effectiveness of the application of a combination of the preparation of micronized purified flavonoid fraction (MPFF) and elastic compression in patients with acquired lymphostasis. MATERIALS AND METHODS: Sixty patients with stage II secondary lower limb lymphedema according to М. Foeldi were included. The patients were divided into two groups through randomization with the envelope method. The first group (n = 30) was subjected to a conservative treatment (MPFF, 1000 mg/day) coupled with elastic compression (3rd class compression stockings). The second group was given compressive therapy (third-class compression stockings). The patients were physically examined through the measurement of the circumference of the limb at different levels. RESULTS: In the first group, the circumference of the lower third of the shin decreased by 8.15% (p = 0.005) after 1 month and by the end of treatment by 10.6% (p 0.001), of the middle third of shin by 3.15% (p = 0.001) and 4.78% (p 0.001), and of the upper thirdby 4.08% (p 0.001) and 5.99% (p 0.001). By the end of the observation period (3 months), the circumference of the lower third of the shin in the second group (29.68 4.67 cm) was significantly greater than that in the first group (26.65 2.92 cm, p = 0.035). No adverse reactions were observed in the MPFF group. CONCLUSIONS: The volume of the lower limbs of patients with acquired lymphedema decreased after using a combination of MPFF and elastic compression to a larger extent than after the isolated use of elastic compression. Patients taking MPFF had a positive clinical effect without adverse reactions. Therefore, MPFF could be used in the pharmacotherapy of secondary lymphedema of the lower limbs.

Comparative effects of powder, aqueous and methanolic extracts of purslane (Portulaca oleracea L.) on growth performance, antioxidant status, abdominal fat deposition and plasma lipids in broiler chickens

2019 ◽  
Vol 59 (1) ◽  
pp. 89 ◽  
Author(s):  
Mahmood Habibian ◽  
Ghorbanali Sadeghi ◽  
Ahmad Karimi
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Plasma Levels ◽  
Broiler Chickens ◽  
Antioxidant Status ◽  
Density Lipoprotein ◽  
Abdominal Fat ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Dietary Treatments

This study was performed to evaluate the comparative effects of dietary supplementation of dried purslane powder (PP), purslane aqueous extract (PAE) and purslane methanolic extract (PME) on performance, antioxidant status, carcass traits and selected plasma lipid parameters in broiler chickens. In total, 420 1-day-old male broiler chicks were divided into seven treatments for 49 days as follows: control (basal diet), basal diets plus 1500 or 3000 mg/kg of PP (PP1500 and PP3000 respectively), basal diets plus 150 or 300 mg/kg of PAE (PAE150 and PAE300 respectively) and basal diets plus 150 or 300 mg/kg of PME (PME150 and PME300 respectively). During the total period of the experiment (0–49 days of the experiment), birds receiving the PP3000 diet had higher (P < 0.05) bodyweight gain and a lower feed conversion ratio compared with those fed other diets. At 24 and 49 days of the experiment, birds receiving the PP3000 diet showed greater (P < 0.05) plasma and liver activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and lower (P < 0.05) plasma and liver levels of malondialdehyde compared with other dietary treatments. Additionally, at 24 days of the experiment, birds receiving the PP3000 diet had a greater (P < 0.05) liver catalase activity than those receiving other dietary treatments. In addition, groups receiving the PP1500, PAE300 or PME300 diets showed greater (P < 0.05) plasma and liver activities of superoxide dismutase, catalase and glutathione peroxidase, as well as lower (P < 0.05) plasma and liver levels of malondialdehyde compared with the control group. At 24 days of the experiment, birds receiving the PP1500 or PP3000 diets showed greater (P < 0.05) jejunal activities of superoxide dismutase, catalase and glutathione peroxidase than other groups. At 49 days of the experiment, birds receiving the PP3000 diet showed greater (P < 0.05) jejunal activities of superoxide dismutase and glutathione peroxidase compared with the control group. Additionally, at both 24 and 49 days of the experiment, groups receiving the PP3000 diet had lower (P < 0.05) jejunal levels of malondialdehyde compared with the control group. At 49 days of the experiment, birds receiving the PP3000 diet had a lower (P < 0.05) relative weight of abdominal fat compared with those receiving the other dietary treatments. Moreover, groups that consumed the PP1500, PAE300 or PME300 diets showed lower (P < 0.05) relative weights of abdominal fat compared with the control group. Groups fed PP, PAE or PME treatments showed lower (P < 0.05) plasma levels of triglycerides, total cholesterol and low-density lipoprotein cholesterol, and higher (P < 0.05) plasma levels of high-density lipoprotein cholesterol than the control group at 24 and 49 days of the experiment, with the most pronounced effects observed in those receiving the PP3000 treatment. In conclusion, PP showed more beneficial effects than PAE and PME, and 3000 mg/kg was the best inclusion level of PP in broiler chicken diets.

scholarly journals Protein Damage and Antioxidant Status Alterations Caused by Oxidative Injury in Chronic Myeloid Leukemia

2016 ◽  
Vol 27 (2) ◽  
pp. 55 ◽  
Author(s):  
Deepti Pande ◽  
Reena Negi ◽  
Ranjana S. Khanna ◽  
Hari D. Khanna
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Chronic Myeloid Leukemia ◽  
Glutathione Peroxidase ◽  
Myeloid Leukemia ◽  
Antioxidant Status ◽  
Philadelphia Chromosome ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Cellular Damage

Objective: To evaluate the oxidative stress and antioxidant defense in patients with chronic myeloid leukemia.Background: Chronic myeloid leukemia is a myeloproliferative disorder associated with a characteristic chromosomal translocation called the Philadelphia chromosome. Reactive oxygen species and other free radicals mediate phenotypic and genotypic changes leading from mutation to neoplasia in all cancers, including chronic myeloid leukemia. We evaluated patients with chronic myeloid leukemia by observing their oxidative status and antioxidant defense.Methods: Using serum from 40 clinically diagnosed cases of chronic myeloid leukemia as well as 40 healthy controls, we measured the concentration of thiobarbituric acid, levels of protein carbonylation, total antioxidant status, catalase, superoxide dismutase, glutathione peroxidase, vitamins A and E, and the trace elements zinc, magnesium, and selenium. Results: We found significantly increased levels of serum malonyldialdehyde and protein carbonyl in patients with chronic myeloid leukemia in comparison to healthy individuals, and significantly decreased levels of the antioxidants and micronutrients thiobarbituric acid, catalase, superoxide dismutase, glutathione peroxidase, vitamins A and E, zinc, magnesium, and selenium. These data suggest cellular damage occurring at the level of lipids and proteins.Conclusion: These findings indicate a link between low levels of antioxidants and cellular damage in patients with chronic myeloid leukemia, supporting the idea that oxidative stress may play a role in the pathogenesis of chronic myeloid leukemia.

scholarly journals Effects of β-carotene on cell viability and antioxidant status of hepatocytes from chronically ethanol-fed rats

2004 ◽  
Vol 92 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Suh-Ching Yang ◽  
Chi-Chang Huang ◽  
Jan-Show Chu ◽  
Jiun-Rong Chen
Keyword(s):  
Superoxide Dismutase ◽  
Lactate Dehydrogenase ◽  
Glutathione Peroxidase ◽  
Cell Viability ◽  
Antioxidant Status ◽  
Lipid Peroxides ◽  
Control Group ◽  
Group Activities ◽  
Ethanol Group ◽  
Β Carotene

The purpose of the present study was to evaluate the effects of β-carotene on the cell viability and antioxidant status of hepatocytes from chronically ethanol-fed rats. Rats in the ethanol group were given an ethanol-containing liquid diet that provided 36% of total energy as ethanol, while rats in the control group were fed an isoenergetic diet without ethanol. After 4 weeks, hepatocytes were taken out and cultured for 24 h. Hepatocytes from the rats in the control and ethanol groups were cultured in medium without (HC, HE) or with β-carotene (HC+B, HE+B). The results showed that lactate dehydrogenase leakage was significantly increased in the HE compared with that in the HC group. However, lactate dehydrogenase leakage of the HE+B group was similar to that of the HC group. When compared with the HC group, activities of glutathione peroxidase and catalase in the HE group were significantly decreased by 54 and 31%, respectively. Catalase activity in the HE+B group was significantly increased by 61% compared with that in the HE group. However, activities of glutathione reductase and superoxide dismutase showed no difference among the groups. The level of glutathione in the HC+B and HE+B groups was significantly increased to 155 and 143% compared with those in the HC and HE groups, respectively. The concentration of lipid peroxides showed no difference among the groups. The present results demonstrate that β-carotene improved the cell viability of hepatocytes, and increased catalase activities and glutathione levels in hepatocytes from chronically ethanol-fed rats.

Estrogen administration, postexercise tissue oxidative stress and vitamin C status in male rats

1998 ◽  
Vol 76 (10-11) ◽  
pp. 952-960 ◽  
Author(s):  
Peter M Tiidus ◽  
Eric Bombardier ◽  
Nick Hidiroglou ◽  
Rene Madere
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Vitamin C ◽  
Glutathione Peroxidase ◽  
Antioxidant Status ◽  
Male Rats ◽  
Glutathione Status ◽  
Estrogen Administration ◽  
Tissue Vitamin

Estrogen can putatively act as an antioxidant and protect tissues from exercise-induced oxidative stress. To test the in vivo efficacy of estrogen, the effects of 2 weeks of daily estrogen (40 µg·kg-1 body weight beta-estradiol 3-benzoate) injection on indices of immediate postexercise oxidative stress and antioxidant status were determined in adult male rats, with and without 8 weeks of prior dietary vitamin E deprivation. The treadmill running protocol (60 min at 21 m·min-1, 12% grade) induced significant oxidative stress as indicated by muscle glutathione status. Estrogen administration had little effect on postexercise tissue glutathione status, superoxide dismutase and glutathione peroxidase activity, and vitamin E levels. Estrogen administration induced significant reductions in muscle, liver, and heart vitamin C concentrations following exercise, as well as in unexercised male rats. Tissue vitamin C loss was not directly mediated through liver glycogen or glutathione status. Thus, estrogen administration generally did not appear to influence postexercise tissue indices of oxidative stress or antioxidant status and may have contributed to a decline in overall antioxidant protection by inducing losses in tissue vitamin C content.Key words: glutathione, vitamin E, muscle, superoxide dismutase, glutathione peroxidase.

scholarly journals Influence of Classical Massage on Biochemical Markers of Oxidative Stress in Humans: Pilot Study

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Zofia Skubisz ◽  
Daria Kupczyk ◽  
Aleksander Goch ◽  
Marcin Siedlaczek ◽  
Łukasz Sielski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Healthy Volunteers ◽  
Biochemical Markers ◽  
Massage Therapy ◽  
Human Organism ◽  
Single Session ◽  
Glutathione S Transferase ◽  
Markers Of Oxidative Stress

Classical massage is one of the most popular forms of conservative treatment in various diseases. Despite the wide scope of research, the mechanisms of massage are not fully known and understood. Apart from the well-described effects on individual body systems, there are few scientific reports on the effects of massage on the human body at the subcellular level. The study was designed to assess changes in oxidative stress parameters in healthy volunteers after a single session of classical massage. 29 healthy volunteers aged 22.24 ± 3.64 participated in the study. Before and 30 minutes after the massage procedures, blood samples were taken by experienced personnel. Biochemical markers of oxidative homeostasis were assessed with highly specific methods for each parameter: oxidase ceruloplasmin, glutathione, malondialdehyde, glutathione peroxidase, glutathione S-transferase, and superoxide dismutase. The study demonstrates that massage therapy caused statistically significant decrease in the concentration of glutathione peroxidase (red blood cells) and increase in the level of glutathione peroxidase (plasma), superoxide dismutase, and malondialdehyde. In contrast, statistically significant changes in the hematocrit, glutathione, NO2-/NO3-, and oxidase ceruloplasmin were not observed. The results show that complex influence of classical massage therapy on human organism may be reflected in parameters of the oxidative stress. To understand this mechanism clearly, further research is needed.

scholarly journals Management of chronic venous insufficiency of the lower extremities

2018 ◽  
pp. 36-41
Author(s):  
M. N. Kudykin
Keyword(s):  
Mechanism Of Action ◽  
Chronic Venous Insufficiency ◽  
Venous Insufficiency ◽  
Clinical Manifestations ◽  
Lower Extremities ◽  
Venous Diseases ◽  
Micronized Purified Flavonoid Fraction ◽  
Flavonoid Fraction

The article presents up-to-date information about epidemiology, pathogenesis and clinical manifestations of chronic venous diseases and chronic venous insufficiency of the lower extremities. It discusses the mechanism of action of various phlebotrophic drugs intended for the treatment of symptoms of chronic venous diseases and chronic venous insufficiency and describes the advantages of micronized purified flavonoid fraction for the treatment of all clinical classes of chronic venous diseases.

scholarly journals Use of Lactobacillus farciminis to improve antioxidant status of Tuj lambs

2021 ◽  
Vol 51 (4) ◽  
pp. 505-512
Author(s):  
O. Durna Aydın ◽  
G. Yıldız ◽  
O. Merhan ◽  
S. Ulufer Bulut ◽  
A. Kanıcı Tarhane ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Status ◽  
Growth Traits ◽  
Live Weight ◽  
Bodyweight Gain ◽  
Probiotic Supplementation ◽  
Subsequent Period ◽  
Preweaning Period

The aim of this study was to investigate the effects of Lactobacillus farciminis on growth traits and antioxidant status in preweaning and postweaning Tuj lambs. Twenty lambs were divided into four groups, regardless of gender, with a mean live weight of 7.81 ± 0.50 kg. At the start of the experiment, the average age of the lambs was seven days. During the six-week preweaning period, control (C) lambs were fed with colostrum only, and Lactobacillus farciminis was given orally to the treated lambs at 1 g/day/lamb (L1), 2 g/day/lamb (L2) or 4 g/day/lamb (L3). The experiment continued for a total of 22 weeks. During the first six weeks, bodyweight (BW) increased significantly in L1 at the sixth week. Also during this period, bodyweight gain (BWG) in L2 at 2 - 3 weeks and in L3 at 5 - 6 weeks differed from C. In the subsequent period, BW and BWG were not affected by probiotic supplementation. The effects of probiotic supplementation on malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were significant throughout the experiment, with the effect on glutathione (GSH) also being important in the first six weeks. Thus, Lactobacillus farciminis provided orally to Tuj breed lambs could be used to improve their antioxidant status without compromising growth.

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