scholarly journals Specific features of the adhesion process of the abdominal cavity in children with different degrees of connective tissue dysplasia

2020 ◽  
Vol 10 (4) ◽  
pp. 381-389
Author(s):  
Sergey V. Minaev ◽  
Sergey I. Timofeev ◽  
Alina N. Grigorova ◽  
Oksana V. Vladimirova ◽  
Elena I. Pashneva ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Clinical Course ◽  
Abdominal Cavity ◽  
Type Iii Collagen ◽  
Type I ◽  
Type Iii ◽  
Connective Tissue Dysplasia ◽  
Group 2 ◽  
Group 1

Aim. To assess adhesions of the abdominal cavity in children with varying degrees of connective tissue dysplasia (CTD) severity. Methods. A total of 91 children with average age of 10.6 1.4 years were observed from 2005 to 2019. Composed of 53 boys (58.2%) and 38 girls (41.8%). Patients were divided into two groups: group 1 (69 children without CTD) and group 2 (22 children with CTD). Clinical data and screening cards were used to diagnose DST. All patients underwent an assessment for the clinical course, a macroscopic visual assessment adhesive process severity in the abdominal cavity according to three different scales (Nair, F. Coccolini, N.I. Ayushinova). Immunohistochemistry was performed against collagen I, III, IV type, Laminin 1, angiogenesis factors, transforming growth factor beta (TGF-), and Fibroblast Growth Factor Receptor 1 (FGFR1). A semi-quantitative counting method was used to record the relative number of immunopositive structures. Statistical analysis was carried out by methods of variation statistics using the Chi-square test and MannWhitney U-tests, as well as the Spearman rank correlation method for the reliability of differences between groups. Results. In the adhesive process visualization, the N.I. Ayushinov scale was revealed to be the most informative, showing the average score of 7 0.8 points in group 2 and 14 1.2 in group 1 (rs 0.35; p 0.05). In group 2, the adhesive material showed no (56%) or chaotic fragments (44%) of type I collagen, and the ratio of type I to type III collagen is 2.7: 5.1. In group 1, the ratio of type I to type III collagen is 5.9:1.8. The ratio of collagen IV to type I is 6.5: 2.9. Both groups have a moderate (++) amount of TGF-. TGF- is positive with macrophages. FGFR1 was found in the control group (++++). A positive response was seen in fibroblasts and macrophages (U = 79.00; p = 0.006). Statistically significant analyzes of vascular endothelial growth factor in compared groups (rs = 0.632, p 0.001) had a positive correlation.. Conclusion. Thus, the study showed features of clinical course and morphological changes during the development of adhesions in the abdominal cavity in children with varying degrees of severity of CTD. Data obtained dictate the need for an individual approach in predicting adhesive disease, as well as targeted preventive care.

scholarly journals Connective tissue dysplasia in the genesis of cervical incompetence

2021 ◽  
Vol 15 (1) ◽  
pp. 41-50
Author(s):  
E. V. Fotina ◽  
R. R. Zakirova ◽  
M. V. Alekseenkova ◽  
O. B. Panina
Keyword(s):  
Connective Tissue ◽  
Preterm Labor ◽  
Clinical Signs ◽  
Pregnancy And Childbirth ◽  
Cervical Incompetence ◽  
Connective Tissue Dysplasia ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim: to study a role of undifferentiated connective tissue dysplasia (uCTD) in etiology of cervical incompetence and its effect on pregnancy and childbirth course.Material and Methods. There were enrolled 60 patients with cervical incompetence. Patients were divided into 3 groups according to the modified CTD scale: group 1 - patients without uCTD (n = 21); group 2 - patients with mild uCTD (n = 25); group 3 - patients with moderate-to-severe uCTD (n = 14). Intensity of CTD clinical signs was assessed based on health status, gynecological and obstetric history, the course of pregnancy, labor and the postpartum period.Results. It was found that patients with more prominent CTD developed earlier (r-Spearman = -0.26) and more marked (r-Spearman = -0.29) cervical shortening and opening of the internal orifice of the uterus (r-Spearman = 0.28). It was also noted that likelihood of occurring preterm labor was significantly higher in patients with CTD (p = 0.02).Conclusion. The data obtained evidence that uCTD affects intensity of manifested cervical insufficiency and rate of preterm delivery.

scholarly journals Skin biopsy as a method for determining indications for preventive endoprosthetics of the anterior abdominal wall

2020 ◽  
pp. 46-53
Author(s):  
Viktor A. Lazarenko ◽  
Sergey V. Ivanov ◽  
Ilya S. Ivanov ◽  
Evgeny G. Ob’edkov ◽  
Nikolay N. Grigor'ev ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Skin Biopsy ◽  
Abdominal Wall ◽  
Anterior Abdominal Wall ◽  
Polarized Light ◽  
Structural Features ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Connective Tissue Dysplasia

The aim is to examine the effectiveness of preoperative skin biopsy method to determine indications for preventive endoprosthesis implantation of the anterior abdominal wall based on structural features of connective tissue. Materials and methods. The study included 17 patients who had been planned to undergo a scheduled surgery such as video laparoscopic cholecystectomy. The histological material was collected with DERMO PUNCH biopsy needles in an outpatient clinic. Using polarized light microscopy we inspected histological slices coloured with Sirius red. After that we analyzed histological sections including the ratio of collagen fibers, the density of connective tissue and the number of interfibrous spaces. Results. All the studied features of connective tissue dysplasia had been registered among 18% of patients; significant differences (p ≤0.05) had been identified among other 24% of patients. In patients with identified signs of connective tissue dysplasia, an increase in type III collagen fibers and a decrease in type I fibers were revealed, which directly resulted in a decrease in their ratio. In addition, this category of patients noted a decrease in the density of connective tissue and a significant number of interfibrous spaces. Conclusion. Skin biopsy is a safe and informative method to reveal patients with predisposition to the formation of hernias of the anterior abdominal wall based on structural features of connective tissue and to determine indications for preventive endoprosthetic implantation. This method will reduce the frequency of postoperative ventral hernias, so the effectiveness of the treatment will definitely increase.

scholarly journals Comparative Evaluation of Oral Fluid Microcrystallization Index in Patients with Maxillofacial and Neck Boils in Different Approaches of Post-Operative Treatment

2021 ◽  
Vol 6 (3) ◽  
pp. 285-292
Author(s):  
M. S. Fleryanovitch ◽  
◽  
I. O. Pohodenko-Chudakova ◽  
◽  
◽  
...  
Keyword(s):  
Oral Fluid ◽  
Standard Treatment ◽  
Control Group ◽  
Type I ◽  
Type Ii ◽  
Maxillofacial Region ◽  
Type Iii ◽  
Group 2 ◽  
Group 1

The purpose of the study is to study the index of microcrystallization of oral fluid in patients with boils of the maxillofacial region, to carry out a comparative assessment of the effectiveness of postoperative treatment of this disease using a standard rehabilitation treatment and the same treatment combined with electroacupuncture (EAT). Materials and methods. We observed 40 patients with boils of the maxillofacial region aged 18-58 years old who were divided into two groups of 20 people in each group after primary surgical treatment (PST) of the infectious and inflammatory focus. Group 1 received a standard anti-inflammatory therapy in the postoperative period. In group 2, the standard treatment was combined with electroacupuncture. The study involved 20 practically healthy people (a control group). The microcrystallization index (MCI) was determined by the method of I.O. Pohodenko-Chudakova, Yu.M. Kazakova, N.D. Pokhodenko (2011). In the control group, the microcrystallization index was determined once, in groups 1 and 2 – twice (at the patient's admission to the hospital and on the 10th day after the primary surgical treatment). The obtained data was processed statistically using the package of application tables "Statistica 10.0" Results and discussion. In the control group, type I of microcrystallization was determined in 72 (40.0%) quadrants, type II – in 98 (54.4%) quadrants, and type III – in 10 (5.6%) quadrants. In group 1 patients in the 1st study, type I of microcrystallization was in 17 (9.4%) quadrants, type II – in 53 (29.5%) quadrants, and type III – in 110 (61.1%) quadrants. In group 2, type I of microcrystallization was detected in 11 (6.1%) quadrants, type II – in 49 (27.2%) quadrants, and type III – in 120 (66.7%) quadrants. On the 10th day of the follow-up period in patients of group 1, type I of crystal formation was determined in 66 (36.7%) quadrants, type II – in 75 (41.7%) quadrants, type III – in 39 (21.6%) quadrants. During the same follow-up period, type I was found in 66 (36.7%) quadrants, type II – in 93 (51.7%), and type III ‒ in 21 (11.6%) quadrants. The microcrystallization index value of group 1 decreased during standard treatment, but it didn’t have directed signs (χ2=7.0; p=0.316). The microcrystallization index in the 2nd study was significantly different from the control (U=0.0; p <0.001) and therefore did not reach the normal values. According to the data of group 2, the revealed positive dynamics had directed signs (χ2=20.0; p <0.001). The microcrystallization index at the end of treatment had no significant differences with the control (U=99.5; p=0.007). Conclusion. The study proves the advantage of the results of treatment of a group of patients who were treated with electroacupuncture as well as the possibility of using the microcrystallization index of oral fluid both for diagnosing the severity of the general condition of a patient with a boil of the maxillofacial region and predicting the course of the disease

scholarly journals Expression of collagens in the damage area at abdominal adhesions

2017 ◽  
Vol 2 (6) ◽  
pp. 188-192
Author(s):  
Irina Shurygina ◽  
Irina Shurygina ◽  
Mikhail Shurygin ◽  
Mikhail Shurygin ◽  
Lyubov Rodionova ◽  
...  
Keyword(s):  
Abdominal Cavity ◽  
Immunofluorescent Staining ◽  
Type Iii Collagen ◽  
Type I ◽  
Damage Area ◽  
Type Iii ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Type V ◽  
Adhesion Process

Background. Postoperative adhesions are a serious problem in surgery. However, at the present time molecular mechanisms of the adhesion process are insufficiently studied. Aim. To study the dynamics of expression of genes encoding the synthesis of collagen in case of damage to the serosa on the example of the peritoneum in conditions of aseptic inflammation. Materials and methods. Aseptic inflammatory process in the abdominal cavity was modeled (Wistar rats, n = 40). A micro- and macroscopic picture of the damage area was studied. Immunofluorescent staining for Type I collagen (Col 1A1) was performed. The expression of genes encoding collagen of different types was evaluated using the RT 2 - Profiler PCR kit Array Rat Wound Healing. Results. It has been established that the adhesion process with peritoneal damage in aseptic conditions reaches its maximum by the 30 th day of observation. The same period coincides with the maximum of collagen synthesis in fibro- blasts in the repair area, revealed by immunofluorescence study. The interrelation of synthesis of type I and III collagens went as expected – the onset of expression of type III collagen (from day 3) is ahead of the expression of collagen type I (from day 7). Peak gene expression of collagens type I, Alpha-1 and -2; type III Alpha-1, type IV Alpha-1 and -3, type V Alpha-1, -2 and -3; type XIV Alpha-1 (Col14a1) falls on the 14th day. For the first time, active involvement of type V alpha-3 collagen in the adhesion process was noted - we detected both early (from day 1) and maximum intensive (up to 166.96 times increase in comparison with intact animals). Conclusion. Perhaps, the hyperexpression of collagen V alpha-3 that we revealed is an important link in the pathogenesis of adhesion in the abdominal cavity.

scholarly journals Immunocytochemical study of collagen in epidermal growth factor (EGF)-treated osteoblastic cells.

1984 ◽  
Vol 32 (11) ◽  
pp. 1231-1233 ◽  
Author(s):  
Y Osaki ◽  
M Tsunoi ◽  
Y Hakeda ◽  
K Kurisu ◽  
M Kumegawa
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Type I Collagen ◽  
Osteoblastic Cells ◽  
Type Iii Collagen ◽  
Type I ◽  
Immunocytochemical Study ◽  
Type Iii ◽  
Epidermal Growth ◽  
And Control

The alteration of collagen components in clone MC3T3-E1 cells by epidermal growth factor (EGF) was investigated immunocytochemically, using antibodies to type I and type III collagens. EGF transformed those cells that had become more slender than those of control cultures. Type I and type III collagens were observed in the same cells in both EGF-treated and control cultures. Type I collagen was decreased by EGF, whereas type III collagen appeared to be increased. However, no cells with only type III collagen were observed, suggesting that EGF influences collagen metabolism in clone MC3T3-E1 cells.

Methionine-Specific Staining of Collagen

1982 ◽  
Vol 40 ◽  
pp. 294-295
Author(s):  
E.M. Kuhn ◽  
K.D. Marenus ◽  
M. Beer
Keyword(s):  
Amino Acids ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Electron Microscopic ◽  
Good Correspondence ◽  
Specific Staining ◽  
Type Iii ◽  
Different Types ◽  
Sulfur Containing

Fibers composed of different types of collagen cannot be differentiated by conventional electron microscopic stains. We are developing staining procedures aimed at identifying collagen fibers of different types.Pt(Gly-L-Met)Cl binds specifically to sulfur-containing amino acids. Different collagens have methionine (met) residues at somewhat different positions. A good correspondence has been reported between known met positions and Pt(GLM) bands in rat Type I SLS (collagen aggregates in which molecules lie adjacent to each other in exact register). We have confirmed this relationship in Type III collagen SLS (Fig. 1).

scholarly journals Subpopulations of rat lung fibroblasts with different amounts of type I and type III collagen mRNAs.

1990 ◽  
Vol 265 (11) ◽  
pp. 6286-6290
Author(s):  
E Breen ◽  
V M Falco ◽  
M Absher ◽  
K R Cutroneo
Keyword(s):  
Lung Fibroblasts ◽  
Type Iii Collagen ◽  
Type I ◽  
Rat Lung ◽  
Type Iii

Plasma concentration and uterine and ovarian expressions of insulin-like growth factor-2 in dogs with cystic endometrial hyperplasia–pyometra

2021 ◽  
Author(s):  
Nilgün Gültiken ◽  
Murat Yarim ◽  
Gül Fatma Yarim ◽  
Mahmut Sözmen ◽  
Elvan Anadol ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Growth Factor ◽  
Endometrial Hyperplasia ◽  
Endocrine Cells ◽  
Inflammatory Process ◽  
Plasma Concentrations ◽  
Insulin Like Growth Factor ◽  
Healthy Dogs ◽  
Group 2 ◽  
Group 1

AbstractThe objective of this study was to investigate the plasma concentrations of insulin-like growth factor-2 (IGF-2) as well as its expression in the uterus and ovary of healthy dogs and those with cystic endometrial hyperplasia (CEH)–pyometra complex. Group 1 (n = 10) included bitches with open cervix pyometra, while Group 2 (n = 7) consisted of clinically healthy bitches in dioestrus. The number of IGF-2 immunopositive interstitial cells was significantly higher in Group 1, whereas in Group 2 there were only two cases in which a few cells were IGF-2 immunopositive. IGF-2 immunopositivity was observed in the endometrial glandular epithelium in both groups. Additionally, interstitial fibroblasts and macrophages in the endometrium were also positive in Group 1. The concentration of plasma IGF-2 was higher in Group 1 than in Group 2 (P < 0.05). The concentration was positively correlated with IGF-2 expression in the endometrial glands (r = 0.926; P < 0.001) in Group 1. However, a negative correlation was present between plasma IGF-2 concentration and IGF-2 expression in the interstitial endocrine cells of the ovary in Group 1 (r = −0.652; P < 0.05). The results suggest that IGF-2 plays an important role during the inflammatory process occurring in bitches with CEH–pyometra complex as well as in the endometrium of healthy bitches in dioestrus.

Infliximab Increases the Tissue Contents of Type-I and Type-III Collagen in Colorectal Segments Without Fecal Stream After Hartmann’s Procedure

2021 ◽  
Author(s):  
Bruna Zini de Paula Freitas ◽  
Fábio Guilherme Campos ◽  
Danilo Toshio Kanno ◽  
Andress Godoy Delben ◽  
José Aires Pereira ◽  
...  
Keyword(s):  
Hartmann’S Procedure ◽  
Type Iii Collagen ◽  
Type I ◽  
Fecal Stream ◽  
Type Iii ◽  
Hartmann's Procedure ◽  
Tissue Contents
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