scholarly journals Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (Cajanus cajan (L.) Millsp)

2014 ◽  
Vol 21 ◽  
pp. 41-49 ◽  
Author(s):  
M. Ariraman ◽  
S. Gnanamurthy ◽  
D. Dhanavel ◽  
T. Bharathi ◽  
S. Murugan
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Cajanus Cajan ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Mutagenic Effect ◽  
Pigeon Pea ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Vigour Index

In the present investigation the seeds of Pigeon pea (Cajanus cajan (L.) Millsp) were treated with different doses of gamma radiation (05KR, 10KR, 15KR, 20KR, 25KR, 30KR, 35KR, 40KR, 45KR, and 50KR) and concentration of Ethyl Methane Sulphonate (05mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM, and 50mM) for studying seed germination, seedling height, (shoot and root), seedling injury, seedling vigour index, and seedling survival of plants at 30th day. The seed germination percentage was decreased with increased in the concentration/doses when compared to control. The LD50 (Lethal dose) value was determined based upon the seed germination percentage. The 50 percentage of seed germination and reduction was observed in 20KR of gamma rays and 25mM of EMS and it is considered as LD50 value for both the treatments. The decrease in seed germination was more prominent with gamma rays than that of EMS treatments. The seedling parameters of gamma rays and EMS treated seedlings were progressively decreased with increase dose/concentration in all mutagenic treatments when compared to control. The maximum seedling parameters were observed in 05KR of gamma rays and 05mM of EMS. Minimum seedling parameters were observed in 50mM of EMS and 50KR of gamma rays respectively.

Lethal Dose (LD50) Fixation and Sensitivity of Fenugreek (Trigonellafoenum-graecumL.) to Gamma Radiation for Induction of Mutation

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
ANURADHA PATEL ◽  
POONAM VERMA ◽  
SHARDA CHOUDHARY ◽  
ARVIND KUMAR VERMA
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Growth Parameters ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Mutation Breeding ◽  
Vigour Index ◽  
Small Flower ◽  
Dose Dependent

Fenugreek (Trigonella foenum-graecumL.) is an annual crop, mainly used as a spiceand leafy vegetable crop in many parts of the world. Classical breeding in fenugreek is restricted due to its low genetic variability and small flower size which hamper manual emasculation and pollination. Mutation breeding is an effective way to enrich genetic variability in crop plants. An experiment was conducted to determine the lethal dose of the physical mutagen gamma rays in fenugreek. The dry seeds of fenugreek were exposed to different doses of gamma rays i.e. 150Gy, 200Gy, 250Gy, 300Gy and 350Gy. These irradiated seeds were sown in the Petri plates with non-irradiated seeds (control). As the dose of gamma rays increased, there was a decrease in germination percentage, seedling survival, root length, shoot length and vigour index. Among five doses of gamma rays, the maximum seed germination was observed at lowest dose 150Gy (93%), followed by 200Gy (83%), 250Gy (76%), 300Gy (76%) and 350Gy (64%). The seedling survival was decreased from 90% (in control) to 56% in 350Gy dose of gamma rays. The gamma rays dose of 150Gy gave stimulatory effect on seedlings growth. The growth parameters were dose dependent, as the dose of gamma rays increased from 200Gy to 350Gy. The gamma rays dose of 350Gy showed 64% seeds germination and 56% of seedlings survival. Therefore, it is concluded that the LD50 dose for fenugreek is close to 350Gy. This information would be highly useful for initiating mutation breeding programme in fenugreek

scholarly journals An improved method of seed germination testing in Kabuli chickpea

2018 ◽  
Author(s):  
Basave Gowda ◽  
A. Krishna Naik ◽  
Rakesh . ◽  
C. Mathad ◽  
B.S. Ganiger ◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Improved Method ◽  
Testing Methods ◽  
Seedling Vigour ◽  
Seed Testing ◽  
Testing Method ◽  
Vigour Index ◽  
Paper Method ◽  
Germinated Seeds

An experiment was conducted during 2014-2015 to standardize the seed testing method in Kabuli chickpea MNK-1 by number of seeds and number of germination papers. Among the different testing methods 25 seeds per replication recorded highest seed germination percentage (91.58%), less number of fresh un-germinated seeds (1.91%), abnormal seedlings (4.00%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2215 and 6863), among the number of germination papers used 2+1 as in between paper method(Bottom 2 and one paper above) recorded significantly higher germination percentage (92.12%), less number of fresh un-germinated seeds (1.41%), abnormal seedlings (3.66%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2463 and 6900), when compared to other. Irrespective of number of germination paper and seeds used however, interaction of 25 seeds per replication and 2+1 between paper method of seed germination testing showed significantly germination percentage (96.50%), less number of fresh un-germinated seeds (0.00%), abnormal seedlings (1.00%), diseased seeds (1.00%) and significantly higher seedling vigour index-I and II (2844 and 7792) followed by 50 seeds per replication. 

scholarly journals Effect of Azolla pinnata on Seed Germination, Vigour Index, Biomass and Yield of French Bean (Phaseolus vulgaris)

2015 ◽  
Vol 3 (2) ◽  
pp. 137-141
Author(s):  
G. S Tejaswini ◽  
S Mahadevakumar ◽  
G. R Janardhana
Keyword(s):  
Seed Germination ◽  
Farmyard Manure ◽  
Germination Percentage ◽  
Positive Control ◽  
Negative Control ◽  
French Bean ◽  
Azolla Pinnata ◽  
Seedling Vigour ◽  
Pod Yield ◽  
Vigour Index

A study was conducted to know the effect of Azolla pinnata collected from Hampapura of K.R. Nagar in Mysore district on seed germination, vigour index, biomass and yield of French bean. A total of four different treatments of earthen pots containing soil: sand: farmyard manure (2:1:1) amended with 10g (T1), 25g (T2), 50g (T3) and 100g (T4) of fresh Azolla biomass and pots amended with 10g (T0) of Factamphos served as positive control. Pots without any supplement served as negative control. 10 seeds of French bean variety ‘S 9’ were sown in each pot and normal agronomic practices were followed. Seed germination was determined after eight days of sowing, and after every 15, 30, 60 and 90 days of sowing, vigour index, biomass and pod yield were determined. The results of the study showed that, high percentage seed germination (81.66%) was recorded with the treatment incorporated with 25g (T2) of Azolla biomass followed by 10g (76.33%), 100g (76.00%) and 50g (72.66%) of Azolla biomass respectively. Minimum seed germination percentage of 64.66% and 65.66 % were recorded in respective negative and positive controls. Significant increase in seedling vigour (VI) was recorded in all the treatments compared to controls. Pod yield increased in T2 followed by T0, T1, T3, T4 and untreated respectively after 90 days of sowing. The study showed that Azolla could be used as a supplement for other pulse crops for increased biomass and overall yield of French bean.

Effect of Depth of Sowing on Seed Germination and Seedling Vigour of Juniperus polycarpos C. Koch

2021 ◽  
Vol 43 (1) ◽  
pp. 35-37
Author(s):  
Pitambar Singh Negi ◽  
◽  
Sandeep Sharma ◽  
Jawala Prasad ◽  
Neha Thakur ◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Vigour Index ◽  
Optimum Depth ◽  
Different Depths ◽  
Juniperus Polycarpos

The seeds of Juniperus polycarpos were sown at six different depths in the nursery viz., 0.5cm, 1.0cm, 1.5cm, 2.0cm, 2.5cm and 3.0cm to study their effect on germination and seedling vigour index. Significant differences were observed in germination percentage and seedling vigour index of Juniperus polycarpos seeds sown at different depth in the nursery. The maximum germination of 68.88 per cent was recorded in seeds sown at 1.5cm depth followed by 50.66 per cent germination recorded at 1.0cm depth, 42.00 per cent germination recorded at 0.5cm depth and 32.00 per cent germination recorded at 2.0cm depth. The minimum germination of 20.66 per cent was recorded in seeds sown at 3.0cm depth. Similarly, seeds sown at 1.50cm depth recorded maximum seedling vigour index (1377.38) followed by seeds sown at 1.0cm depth (1077.26) and then by seeds sown at 0.50cm depth (890.73). The seed sown at 3.0cm depth recorded minimum seedling vigour index (458.40). It is recommended that the seeds of Juniperus polycarpos should be sown at optimum depth of 1.50cm in the nursery for getting maximum germination and seedling vigour.

ALLELOPATHIC EFFECTS OF PARTHENIUM HYSTEROPHORUS ON SEED GERMINATION AND SEEDLING GROWTH IN CAJANUS CAJAN

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
G. S. THAKUR
Keyword(s):  
Seed Germination ◽  
Aqueous Extract ◽  
Seedling Growth ◽  
Cajanus Cajan ◽  
Pigeon Pea ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Parthenium Hysterophorus ◽  
Germination Index ◽  
Allelopathic Effects

In this study the allelopathic effects of Parthenium hysterophorus on seed germination and seedling growth in Cajanus cajan (Pigeon pea) was investigated. The dried powdered leaves of Parthenium hysterophorus were soaked in distilled water for 24 hours to obtain aqueous extract of leaves and sterilized seeds were treated with 2%, 4%, 6%,8%, and 10% concentration of extract in triplicate. The germinated seeds were counted every day to observe germination index (G.I.) and mean germination time (MGT). It has been found that germination index (GI) were significantly decreased and MGT were delayed to germinate with increasing concentration. It has been observed that seed germination percentage, root length, shoot length and seedling vigor index were reduced at >2% as compared to control. The 10 % aqueous extract showed completely inhibitory effect on seed germination. The present investigation showed that the leaves aqueous extract of Parthenium hysterophorus had inhibiting effects on seed germination and seedling growth in Cajanus cajan (Pigeon pea).

scholarly journals Optimal Lethal Dose Determination for Gamma Rays and EMS Induced Mutagenesis in TMV7 and SVPR1 Sesame (Sesamum indicum L.) Varieties

2020 ◽  
pp. 136-144
Author(s):  
G. Parthasarathi ◽  
M. Arumugam Pillai ◽  
R. Kannan ◽  
S. Merina Prem Kumari ◽  
Asish K. Binodh
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Probit Analysis ◽  
Dependent Relationship ◽  
Methane Sulphonate ◽  
Induced Mutagenesis ◽  
Dose Dependent

In the present study two sesame varieties viz., TMV7 and SVPR1 were treated with varying doses of gamma rays (250, 300, 350, 400 and 450 Gy) and Ethyl Methane Sulphonate (EMS) of different concentrations viz.,0.20, 0.40 and 0.60%. The seed germination percentage was greatly affected by mutagenic treatment of gamma rays and EMS which showed a negative dose dependent relationship in both the varieties. The expected LD50 values were calculated through probit analysis. The LD50 values for TMV7 and SVPR1 were fixed at 416.86 Gy and 389.04 Gy for gamma rays and 0.490 % and 0.349% for EMS. The germination percentage of SVPR1 was greatly reduced (17.80 & 20.55 %) and the lethal dose to kill fifty per cent of mutated population was lower (6.68% & 28.78%) than that of TMV7 in both gamma ray and EMS treatment. EMS treatment exhibited significant reduction in seed germination (62.16 % & 66.67 %) than gamma irradiation (56.76 % & 54.55 %) in TMV7 and SVPR1 respectively. The study concluded that both the mutagens are effective to produce significant variations in sesame which can be further explored for mutation mapping.

scholarly journals Performance of Bioprimed Chilli Seed under Moisture Stress Condition

2019 ◽  
pp. 1-7
Author(s):  
M. Ananthi ◽  
P. Selvaraju ◽  
K. Sundaralingam
Keyword(s):  
Seed Germination ◽  
Pseudomonas Fluorescens ◽  
Stress Condition ◽  
Trichoderma Viride ◽  
Moisture Stress ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Control Seed ◽  
Vigour Index ◽  
Improve Seed Germination

Laboratory experiment was carried out using ‘PKM 1’ chilli (Capsicum annuum L.) seed to standardize bioprimed seeds under moisture stress condition inorder to improve seed germination and seedling vigour. To induce the moisture stress, optimize the different concentration of water holdings capacity viz., 80, 60, 40 and 20% were used for best bioprimed seed treatment (biocontrol agents (Pseudomonas fluorescens 60% 12 h, Trichoderma viride 60% 9 h) and liquid biofertilizers (Azospirillum 10% 9 h and Phosphobacteria 15% 9h )) along with hydroprimed seed and control seed. Seed bioprimed with Pseudomonas fluorescens 60% for 12 h improved the germination percentage ( 82), root length (14.2 cm), shoot length (6.2 cm), dry matter production (0.0489 g 10 seedlings-1) and vigour index (1673) compared to control seed. Seed biopriming with  Pseudomonas fluorescens 60% for 12 h can be adopted to improve seed germination and seedling vigour under moisture stress condition upto 20%.

scholarly journals The Influence Of Zinc On Seed Germination And Growth In The First Ontogenetic Stages In The Species Cucumis Melo L.

2015 ◽  
Vol 9 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Anişoara Stratu ◽  
Naela Costică
Keyword(s):  
Seed Germination ◽  
Cucumis Melo ◽  
Growth Process ◽  
Lateral Roots ◽  
Tolerance Index ◽  
High Concentration ◽  
Seedling Vigour ◽  
Ontogenetic Stages ◽  
Vigour Index ◽  
Germination And Growth

AbstractThe paper presents the results of a study regarding the influence of treatment with zinc in different concentrations (50 mg∕l, 100 mg∕l, 200 mg ∕l, 300mg/l, 400 mg ∕l, 500 mg/l, 600 mg ∕l) on seed germination and growth in early ontogenetic stages ofCucumis meloL. We analyzed the following indicators: the percentage of germinated seeds; the length of root, the length of the hypocotyl and the length of the seedling; the number of the laterale roots; the tolerance index and the seedling vigor index. The results underline the specific variations of analysed indicators, depending on the concentrations used for the treatments of seeds. The concentrations used for treatment do not influence negatively the seed germination, but affected the seedling growth (especially the root elongation), the formation and growth process of lateral roots and the seedling vigour index. The delay effect of growth process is very pronounced in the case of high concentration.

scholarly journals Physical and Chemical Induced Mutagenesis Study for Identifying Lethality Dose in Chick Pea (Cicer arietinum L.) Var. Co - 4

2015 ◽  
Vol 35 ◽  
pp. 1-5 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Root Length ◽  
Lethal Dose ◽  
Seedling Height ◽  
Growth Reduction ◽  
Chick Pea ◽  
Induced Mutagenesis ◽  
Mutagenesis Study ◽  
Physical And Chemical

The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD50). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M1 generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD50 values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.

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