scholarly journals Physical and Chemical Induced Mutagenesis Study for Identifying Lethality Dose in Chick Pea (Cicer arietinum L.) Var. Co - 4

2015 ◽  
Vol 35 ◽  
pp. 1-5 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Root Length ◽  
Lethal Dose ◽  
Seedling Height ◽  
Growth Reduction ◽  
Chick Pea ◽  
Induced Mutagenesis ◽  
Mutagenesis Study ◽  
Physical And Chemical

The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD50). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M1 generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD50 values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.

scholarly journals Mutagenic Effect of Gamma Rays and EMS on Seed Germination, Seedling Height Reduction and Survivability of Chick Pea (Cicer arietinum L.) Var. Co-4

2014 ◽  
Vol 16 ◽  
pp. 38-43 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan
Keyword(s):  
Seed Germination ◽  
Cicer Arietinum ◽  
Gamma Rays ◽  
Mutagenic Effect ◽  
Seedling Height ◽  
Growth Reduction ◽  
Chick Pea ◽  
Height Reduction ◽  
Effective Doses ◽  
Different Doses

The effect of gamma irradiation and EMS treatment on seed germination and seedling height of Chick pea (Cicer arietinum. L.). In this regard Co-4 variety of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50, and 60 kR) and EMS (10, 20, 30, 40 and 50 mM) for inducing mutation. The effect of mutagen was observed on the basis of percentage of seed germination, seedling height reduction at 15th day and survivability. From the result it was observed that, the percentage of seed germination, seedling height reduction at 15th day and survivability were significantly decreased with increasing doses/concentrations of mutagen. The effective doses/ concentrations which caused 50 % growth reduction were 40kR in gamma rays and 30 mM in EMS.

scholarly journals Optimal Lethal Dose Determination for Gamma Rays and EMS Induced Mutagenesis in TMV7 and SVPR1 Sesame (Sesamum indicum L.) Varieties

2020 ◽  
pp. 136-144
Author(s):  
G. Parthasarathi ◽  
M. Arumugam Pillai ◽  
R. Kannan ◽  
S. Merina Prem Kumari ◽  
Asish K. Binodh
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Probit Analysis ◽  
Dependent Relationship ◽  
Methane Sulphonate ◽  
Induced Mutagenesis ◽  
Dose Dependent

In the present study two sesame varieties viz., TMV7 and SVPR1 were treated with varying doses of gamma rays (250, 300, 350, 400 and 450 Gy) and Ethyl Methane Sulphonate (EMS) of different concentrations viz.,0.20, 0.40 and 0.60%. The seed germination percentage was greatly affected by mutagenic treatment of gamma rays and EMS which showed a negative dose dependent relationship in both the varieties. The expected LD50 values were calculated through probit analysis. The LD50 values for TMV7 and SVPR1 were fixed at 416.86 Gy and 389.04 Gy for gamma rays and 0.490 % and 0.349% for EMS. The germination percentage of SVPR1 was greatly reduced (17.80 & 20.55 %) and the lethal dose to kill fifty per cent of mutated population was lower (6.68% & 28.78%) than that of TMV7 in both gamma ray and EMS treatment. EMS treatment exhibited significant reduction in seed germination (62.16 % & 66.67 %) than gamma irradiation (56.76 % & 54.55 %) in TMV7 and SVPR1 respectively. The study concluded that both the mutagens are effective to produce significant variations in sesame which can be further explored for mutation mapping.

scholarly journals Induced Mutagenesis in Cicer arietinum

2014 ◽  
Vol 12 ◽  
pp. 1-4 ◽  
Author(s):  
L. Mullainathan ◽  
S. Umavathi
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Crop Improvement ◽  
Ethyl Methane Sulphonate ◽  
Chick Pea ◽  
Biological Damage ◽  
Methane Sulphonate ◽  
Dose Concentration ◽  
Induced Mutagenesis ◽  
Effectiveness And Efficiency

The traditional varieties of chick pea have low potentiality and restricted variability with respect to economic characters. Broadening the genetic base for crop improvement can be quickly achieved through induced mutagenesis. The present study was undertaken in order to comparing the effectiveness and efficiency of mutagens on Cicer arietinum. In this regard, Co – 4 variety of chick pea was subjected to different dose/concentration of Gamma rays (20, 30, 40, 50 and 60 kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM) for inducing mutation. Mutagenic effectiveness and efficiency was calculated based on biological damage in M1 and chlorophyll mutations in M2. The results indicated that, mutagenic effectiveness increased with the increase in dose/concentration of mutagen. Intermediate treatments in general were found more efficient in causing less biological damage and inducing maximum amount of mutations. It shows that the chemical mutagens are more effective and efficient than physical mutagen for inducing mutation in Chick pea

scholarly journals Physical and Chemical Mutagenesis in Linum usitatissimum L. to Induce Variability in Seed Germination, Survival and Growth rate traits

2017 ◽  
Vol 7 ◽  
pp. 28 ◽  
Author(s):  
Irfan Ahmad Bhat ◽  
Umar Jan Pandit ◽  
Imtiyaz Ahmad Sheikh ◽  
Zia Ul Hassan
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Linum Usitatissimum ◽  
Dry Weight ◽  
Chemical Mutagenesis ◽  
High Dose ◽  
X Rays ◽  
Linum Usitatissimum L ◽  
Survival And Growth ◽  
Physical And Chemical

The  studies  of  induced  mutation  in  <em>Linum  usitatissimum</em>  L.  were  performed  by  exposing  the  fully  mature  and  healthy  dry  seeds  to  gamma  rays    at  10  and  15  Kr,  X-rays  at  10  and  15  Kr,  hydroxylamine  (HA)  at  0.1%  and  0.2%,  5-amino  acridine  (AA)  at  0.1%  and  0.2%  doses.  The  observations  were  made  for  seed  germination  %,  survival  %  and  growth  characters  such  as  shoot  length,  root  length  and  dry  weight.  Seeds  treated  with  low  dose  of  mutagens  showed  negligible  effect  while  that  with  the  high  dose  exhibit  significant  effects  on  studied  parameters  as  compared  to  control.  Data  obtained  in  this  study  were  statistically  significant  at  5%  level.  The  results  conclude  that  treatments  of  gamma  rays  and  X-rays  were  less  effective  as  compared  to  those  of  HA  and  AA  treatments.

scholarly journals Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (Cajanus cajan (L.) Millsp)

2014 ◽  
Vol 21 ◽  
pp. 41-49 ◽  
Author(s):  
M. Ariraman ◽  
S. Gnanamurthy ◽  
D. Dhanavel ◽  
T. Bharathi ◽  
S. Murugan
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Cajanus Cajan ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Mutagenic Effect ◽  
Pigeon Pea ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Vigour Index

In the present investigation the seeds of Pigeon pea (Cajanus cajan (L.) Millsp) were treated with different doses of gamma radiation (05KR, 10KR, 15KR, 20KR, 25KR, 30KR, 35KR, 40KR, 45KR, and 50KR) and concentration of Ethyl Methane Sulphonate (05mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM, and 50mM) for studying seed germination, seedling height, (shoot and root), seedling injury, seedling vigour index, and seedling survival of plants at 30th day. The seed germination percentage was decreased with increased in the concentration/doses when compared to control. The LD50 (Lethal dose) value was determined based upon the seed germination percentage. The 50 percentage of seed germination and reduction was observed in 20KR of gamma rays and 25mM of EMS and it is considered as LD50 value for both the treatments. The decrease in seed germination was more prominent with gamma rays than that of EMS treatments. The seedling parameters of gamma rays and EMS treated seedlings were progressively decreased with increase dose/concentration in all mutagenic treatments when compared to control. The maximum seedling parameters were observed in 05KR of gamma rays and 05mM of EMS. Minimum seedling parameters were observed in 50mM of EMS and 50KR of gamma rays respectively.

scholarly journals Effect of mutagenic agents on seed germination and vegetative growth of mustard (Brassica rapa)

2014 ◽  
Vol 48 (4) ◽  
pp. 253-256 ◽  
Author(s):  
NE Khan ◽  
N Hassan ◽  
MR Islam ◽  
SK Biswas
Keyword(s):  
Seed Germination ◽  
Gamma Radiation ◽  
Brassica Rapa ◽  
Sodium Azide ◽  
Root Length ◽  
Shoot Length ◽  
Radiation Chemical ◽  
Gamma Irradiated ◽  
Physical And Chemical

The experiment was conducted to study the effect of mutagens (physical and chemical) on seed germination along with root and shoot lengths of two locally collected varieties of Brassica rapa denoted as variety-1 and variety-2 in in vitro condition. Seeds were treated with various concentrations of mutagenic agents; physical (gamma radiation), chemical (sodium azide) and combined (gamma radiation + sodium azide). The doses of gamma radiation were 100Gy and 200Gy, sodium azide concentrations were 100, 300 and 500ppm and for combination, 100Gy and 200Gy gamma irradiated seeds were further treated with 100, 300 and 500ppm sodium azide solution. After 7 days observation, 100% seed germination obtained from 100Gy gamma irradiated seeds of variety-1 and 500ppm sodium azide treated seeds of variety- 2. 100% seed germination was also found in 7 days in physical and chemical mutagen combinedly treated seeds of variety-1 from 100Gy gamma radiation + 100ppm sodium azide, 100Gy gamma radiation + 300ppm sodium azide and 200Gy gamma radiation + 100ppm sodium azide and in variety-2, from 100Gy gamma radiation + 500ppm sodium azide and 200Gy gamma radiation + 500ppm sodium azide. Maximum increased root length (avg. 3.8cm) was obtained from variety-1 combined treated (200Gy gamma radiation + 300ppm sodium azide) seeds and minimum decreased root length (avg. 1.0cm) was from variety-2 combined treated (100Gy gamma radiation + 100ppm sodium azide) seeds. Maximum increased shoot length (avg. 4.7cm) was obtained from variety-1 combined treated (200Gy gamma radiation + 100ppm sodium azide) seeds and minimum decreased shoot length (avg. 1.4cm) was obtained from variety-2 combined treated (100Gy gamma radiation + 100ppm sodium azide) seeds. DOI: http://dx.doi.org/10.3329/bjsir.v48i4.18274 Bangladesh J. Sci. Ind. Res. 48(4), 253-256, 2013

scholarly journals Common bean seed germination and seedling emergence under inoculation with biostimulators

2020 ◽  
Vol 19 (2) ◽  
pp. 168-174
Author(s):  
Antônio Jussiê da Silva Solino ◽  
Juliana Santos Batista de Oliveira ◽  
Maycon Schmitt ◽  
Jéssica Brasau da Silva ◽  
Kátia Regina Freitas Schwan-Estrada
Keyword(s):  
Seed Germination ◽  
Common Bean ◽  
Root Length ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seedling Height ◽  
Bean Seed ◽  
Fresh Weight ◽  
Shoot Fresh Weight ◽  
Shoot Dry Weight

Biostimulating microorganisms have protector effect against pathogenic agents, affect the stand formation, promote plant growth, and increase yield of agricultural crops. Thus, the objective of this work was to evaluate the seed germination and seedling emergence of different common bean cultivars as a function of treatments with Bacillus subtilis BV02 and Trichoderma asperellum BV10. Germination tests in paper rolls and sand were conducted in a completely randomized design, with a 2×4 factorial arrangement consisted of two cultivars, namely Campos Gerais (CG) and Estilo (ES), and four microorganism treatments, namely B. subtilis BV02 (BS), T. asperellum BV10 (TA), B. subtilis BV02 + T. asperellum BV10 (BS+TA), and Control. The variables analyzed were: germination speed index (GSI), emergence speed index (ESI), germination percentage, emergence percentage, root length, seedling height, and root and shoot fresh and dry weights. The CG cultivar had higher GSI, ESI, root length, seedling height, shoot fresh weight, root fresh weight, and shoot dry weight than the ES cultivar in the paper roll and sand tests. The common bean seed inoculation with BS+TA increased seedling height, shoot fresh weight, shoot dry weight, and root length in the ES cultivar. The CG cultivar had higher GSI, ESI, root length, root fresh weight, and root and shoot dry weights than the ES cultivar. The treatment of seeds with BS+TA is recommended to improve the performance of common bean seedlings of the ES cultivar.

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