Lethal Dose (LD50) Fixation and Sensitivity of Fenugreek (Trigonellafoenum-graecumL.) to Gamma Radiation for Induction of Mutation

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
ANURADHA PATEL ◽  
POONAM VERMA ◽  
SHARDA CHOUDHARY ◽  
ARVIND KUMAR VERMA
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Growth Parameters ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Mutation Breeding ◽  
Vigour Index ◽  
Small Flower ◽  
Dose Dependent

Fenugreek (Trigonella foenum-graecumL.) is an annual crop, mainly used as a spiceand leafy vegetable crop in many parts of the world. Classical breeding in fenugreek is restricted due to its low genetic variability and small flower size which hamper manual emasculation and pollination. Mutation breeding is an effective way to enrich genetic variability in crop plants. An experiment was conducted to determine the lethal dose of the physical mutagen gamma rays in fenugreek. The dry seeds of fenugreek were exposed to different doses of gamma rays i.e. 150Gy, 200Gy, 250Gy, 300Gy and 350Gy. These irradiated seeds were sown in the Petri plates with non-irradiated seeds (control). As the dose of gamma rays increased, there was a decrease in germination percentage, seedling survival, root length, shoot length and vigour index. Among five doses of gamma rays, the maximum seed germination was observed at lowest dose 150Gy (93%), followed by 200Gy (83%), 250Gy (76%), 300Gy (76%) and 350Gy (64%). The seedling survival was decreased from 90% (in control) to 56% in 350Gy dose of gamma rays. The gamma rays dose of 150Gy gave stimulatory effect on seedlings growth. The growth parameters were dose dependent, as the dose of gamma rays increased from 200Gy to 350Gy. The gamma rays dose of 350Gy showed 64% seeds germination and 56% of seedlings survival. Therefore, it is concluded that the LD50 dose for fenugreek is close to 350Gy. This information would be highly useful for initiating mutation breeding programme in fenugreek

scholarly journals Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (Cajanus cajan (L.) Millsp)

2014 ◽  
Vol 21 ◽  
pp. 41-49 ◽  
Author(s):  
M. Ariraman ◽  
S. Gnanamurthy ◽  
D. Dhanavel ◽  
T. Bharathi ◽  
S. Murugan
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Cajanus Cajan ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Mutagenic Effect ◽  
Pigeon Pea ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Vigour Index

In the present investigation the seeds of Pigeon pea (Cajanus cajan (L.) Millsp) were treated with different doses of gamma radiation (05KR, 10KR, 15KR, 20KR, 25KR, 30KR, 35KR, 40KR, 45KR, and 50KR) and concentration of Ethyl Methane Sulphonate (05mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM, and 50mM) for studying seed germination, seedling height, (shoot and root), seedling injury, seedling vigour index, and seedling survival of plants at 30th day. The seed germination percentage was decreased with increased in the concentration/doses when compared to control. The LD50 (Lethal dose) value was determined based upon the seed germination percentage. The 50 percentage of seed germination and reduction was observed in 20KR of gamma rays and 25mM of EMS and it is considered as LD50 value for both the treatments. The decrease in seed germination was more prominent with gamma rays than that of EMS treatments. The seedling parameters of gamma rays and EMS treated seedlings were progressively decreased with increase dose/concentration in all mutagenic treatments when compared to control. The maximum seedling parameters were observed in 05KR of gamma rays and 05mM of EMS. Minimum seedling parameters were observed in 50mM of EMS and 50KR of gamma rays respectively.

scholarly journals Optimal Lethal Dose Determination for Gamma Rays and EMS Induced Mutagenesis in TMV7 and SVPR1 Sesame (Sesamum indicum L.) Varieties

2020 ◽  
pp. 136-144
Author(s):  
G. Parthasarathi ◽  
M. Arumugam Pillai ◽  
R. Kannan ◽  
S. Merina Prem Kumari ◽  
Asish K. Binodh
Keyword(s):  
Seed Germination ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Probit Analysis ◽  
Dependent Relationship ◽  
Methane Sulphonate ◽  
Induced Mutagenesis ◽  
Dose Dependent

In the present study two sesame varieties viz., TMV7 and SVPR1 were treated with varying doses of gamma rays (250, 300, 350, 400 and 450 Gy) and Ethyl Methane Sulphonate (EMS) of different concentrations viz.,0.20, 0.40 and 0.60%. The seed germination percentage was greatly affected by mutagenic treatment of gamma rays and EMS which showed a negative dose dependent relationship in both the varieties. The expected LD50 values were calculated through probit analysis. The LD50 values for TMV7 and SVPR1 were fixed at 416.86 Gy and 389.04 Gy for gamma rays and 0.490 % and 0.349% for EMS. The germination percentage of SVPR1 was greatly reduced (17.80 & 20.55 %) and the lethal dose to kill fifty per cent of mutated population was lower (6.68% & 28.78%) than that of TMV7 in both gamma ray and EMS treatment. EMS treatment exhibited significant reduction in seed germination (62.16 % & 66.67 %) than gamma irradiation (56.76 % & 54.55 %) in TMV7 and SVPR1 respectively. The study concluded that both the mutagens are effective to produce significant variations in sesame which can be further explored for mutation mapping.

scholarly journals Effects of gamma rays on germination and growth in Jatropha curcas L.

2015 ◽  
Vol 7 (2) ◽  
pp. 964-969 ◽  
Author(s):  
Dileswar Nayak ◽  
N. S. Patil ◽  
L. K. Behera ◽  
D. B. Jadeja
Keyword(s):  
Leaf Area ◽  
Gamma Rays ◽  
Jatropha Curcas ◽  
Seedling Survival ◽  
Germination Percentage ◽  
Shoot Length ◽  
Growth And Yield ◽  
Jatropha Curcas L ◽  
Number Of Leaves ◽  
Collar Diameter

The present investigation was carried out at the Forestry Research Farm, Navsari Agricultural University; Navsari to evaluate the gamma rays at 10 kR, 20 kR and 30 kR induced variability in Jatropha curcas L. on germination, growth and yield for seven Jatropha genotypes (Phule J-1, Urlikanchan, Hansraj, SKN Big, Chhatrapati, Hansot and MPJ-55). The significantly maximum germination percentage (66.96%), seedling survival (74.18%), seedling collar diameter (0.958cm), shoot length (49.442cm), number of leaves per seedling (7.757) and leaf area (37. 58)was observed in Chhatrapati genotype during nursery stage. While low rate of gamma rays treatment (10 kR) had stimulatory effect for germination percentage, seedling survival, seedling collar diameter, shoot length, number of leaves per seedling and leaf area. However, higher gamma rays doses (30 kR) drastically reduced all studied characters. The interaction effect of genotype and gamma rays were significant for number of leaves per seedling. Further, it was not significant in other traits like germination percentage, seedling survival, seedling collar diameter, shoot length and leaf area.

scholarly journals Studies on Induced Physical and Chemical Mutagenesis in Groundnut (Arachis hypogia)

2015 ◽  
Vol 35 ◽  
pp. 25-35 ◽  
Author(s):  
A. Gunasekaran ◽  
P. Pavadai
Keyword(s):  
Gamma Rays ◽  
Seedling Survival ◽  
Dry Weight ◽  
Mutation Breeding ◽  
Chemical Mutagenesis ◽  
Program Selection ◽  
Methane Sulphonate ◽  
Ld50 Value ◽  
Mutant Lines ◽  
Physical And Chemical

Mutation breeding has been widely used for the improvement of plant characters in various crops. It is a powerful and effective tool in the hands of plant breeders. In any mutation breeding program, selection of an effective and efficient mutagen is very essential to produce high frequency of desirable mutation. Groundnut (Arachis hypogia) var. VRI-2. was treated with different concentration of physical and chemical mutagen namely gamma rays 10, 20, 30, 40, 50 and 60 KR and Ethyl methane sulphonate (EMS). For inducing mutation various concentration of EMS such as 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 % for six hours were applied to 200 seed sample of each concentration and one respective control. The LD50 value was observed in 50% of gamma rays and 0.5 % of EMS. The morphological and yield characters were significantly reduced seed germination, seedling survival, days to first flower, plant height, number of leaves per plant, number of grains per plant, grain length and breath, 100 grains weight, grain yield per plant, fresh and dry weight per plant. The increasing doses/concentration of gamma rays and EMS decreased in phenotypic and yield characters in M1generation. The mutagenized populations showed significantly higher variability in the M2 generation. Mutant lines showing higher yield per plant than the respective parents and checks were isolated in M2 and subsequent generation were significantly more pod yield and yield components than the untreated plants.

scholarly journals Enhancement of Genetic Variability for Yield and Component Traits through Recombination followed by Induced Mutagenesis in Greengram [Vigna radiata (L.) Wilczek]

2020 ◽  
pp. 38-48
Author(s):  
Prasanta Kumar Majhi ◽  
Suma C. Mogali ◽  
L. S. Abhisheka
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Agricultural Research ◽  
Mutation Breeding ◽  
Research Station ◽  
Induced Mutation ◽  
Breeding Lines ◽  
Component Traits ◽  
High Heritability ◽  
Augmented Design

Aim: Greengram is a self-pollinated crop which show very less variability to develop improved varieties through only hybridization or induced mutation breeding. Therefore, we have taken a new pace to create more variability by combining both recombinations with induced mutation through gamma rays irradiation. For this purpose, the F2 seeds were irradiated with gamma rays at BARC, Mumbai and sown to grow the F2M1 generation and subsequently the superior mutant lines with high degree variability with high GCV and genetic advances were selected from F2M2 generation of the mutant population. Methodology: The present investigation was carried out during kharif-2017 and rabi-summer 2017-18 at the experimental plot, All India Coordinated Research Projects (AICRP) on MULLaRP, main Agricultural Research Station, College of Agriculture, University of Agricultural Sciences, Dharwad, Karnataka, India. The experiment was laid out in an augmented design. Results: The mutant progenies obtained from the crosses DGGV-2 × IPM-410-3 and DGGV-2 × SML-1815 in F2M2 generation have shown high PCV and GCV for the characters like plant height, number of clusters per plant and number of seeds per pod etc. when irradiated with 100 kR gamma rays. The mutant breeding lines derived from the crosses DGGV-7 × V-02-709 and DGGV-7 × V-02-802 with irradiation dose of 20 kR, have shown higher number of pods per cluster and higher number of pods per plant with high heritability. More variability was observed with higher dose (100 kR) of mutation even though it showed higher mortality rate. Conclusion: Irradiation of F2 progeny (DGGV-2 × SML-1815) with 100 kR has generated more genetic variability for seed yield per plant (10.8 g), when compared to the check DGGV-2 (4.7 g) and SML-1815 (9.8 g). So, priority should be given to those characters which are having high heritability coupled with high genetic advance as per cent mean to get better selection gains. The breeding lines which showed higher degree of variability can be utilized in the future breeding programme for development of high yielding genotypes.

scholarly journals Potentiality of Bioagents on Seed Quality Enhancement in Chickpea

2014 ◽  
Vol 3 (3) ◽  
pp. 149-153 ◽  
Author(s):  
Venuturla Bharathi ◽  
Kilaru K. Durga ◽  
Madugula S. Rani
Keyword(s):  
Seed Quality ◽  
Growth Parameters ◽  
Germination Percentage ◽  
Quality Enhancement ◽  
Seedling Vigour ◽  
Vigor Index ◽  
Seedling Length ◽  
Vigour Index ◽  
Quality Character ◽  
And Storage

Planting healthy seeds and other inputs have an impact on seed quality, fungal invasion deteriorates the seed quality in soil and storage. Studies on chickpea carried out at Seed Research and Technology Center, Rajendranagar, Hyderabad during spring 2010-11 2011-12 revealed that benomyl was found effective followed by Pseudomonas fluorescens in the inhibition of mycelial growth of Fusarium solani. Maximum germination percentage was recorded with Benomyl (Benomyl 500 WP) @ 2 g/kg seed (treated check) and Tebuconazole @ 1 ml/ kg seed (95 per cent) followed by treatment with P. fluorescens @ 10 g/kg seed along with soil application of P. fluorescens @ 3 kg/acre (94 per cent). Maximization of growth parameters like root length, shoot length and total seedling length were observed with Benomyl @ 2 g/ kg seed  as 17.0 cm, 10.3 cm and 27.3 cm, respectively. Considering seedling vigour index as an important seed quality character, P. fluorescens and Benomyl @ 2 g/kg seed recorded high seedling vigor index. The per cent recovery of infested seeds was found to be low with treated seeds when compared to the control.

Application of mutation breeding techniques in the development of green crop varieties in Sri Lanka: the way forward.

2021 ◽  
pp. 76-82
Author(s):  
Malathy Parasuraman ◽  
Priyantha Weerasinghe
Keyword(s):  
Sri Lanka ◽  
Genetic Variability ◽  
Gamma Rays ◽  
Mutation Breeding ◽  
Iron Toxicity ◽  
Mutant Line ◽  
High Yield ◽  
Rice Mutant ◽  
The 1960S ◽  
The Way

Abstract The Department of Agriculture (DOA) in Sri Lanka initiated mutation breeding in the 1960s with the introduction of a cobalt-60 source. The first rice mutant variety, MI 273, was released for general cultivation in 1971. M1 273, derived from irradiation of the H-4 variety, was identified as a drought-tolerant variety. An indirect rice mutant variety, developed by crossing the short mutant line BW267-3 with a highly adaptable variety, was released as BW 372 in 2013. It is moderately tolerant to blast, bacterial leaf blight, brown plant hopper, gall midge and iron toxicity, and thus increases productivity to 3-4 t/ha on lands prone to iron toxicity. The most popular groundnut variety cultivated in the country, 'Tissa', is a mutant developed by irradiation with gamma-rays at 200 Gy. It showed attributes of high yield, medium maturity (90-100 days) and high oil content (42%). 'Tissa' presently covers 80% of the groundnut cultivated area in Sri Lanka. A sesame mutant line, derived from the variety MI-3 irradiated at 200 Gy with 60Co gamma-rays, was released as 'Malee' (ANK-S2) in 1993. It is a high-yielding variety (1.1-1.8 t/ha) resistant to Phytophthora blight. A cherry-type mutant tomato variety, developed by irradiation of seeds with gamma-rays (320 Gy), was released as 'Lanka Cherry' in 2010. Improved attributes are pear-shaped fruits and bacterial wilt resistance. Narrow genetic variability in many crops is a constraint to the development of new varieties adapted to the changing climate. Hence, the DOA is emphasizing integration of induced mutagenesis in conventional breeding programmes to develop resistant/tolerant varieties having high yield, quality and health-promoting functional properties in field and horticultural crops. The newly installed gamma irradiation chamber facilitates the creation of genetic variability in food crops, thus paving the way for the development of greener varieties.

Effect of gamma-ray irradiation on Escherichia coli motility

2014 ◽  
Vol 9 (10) ◽  
pp. 909-914 ◽  
Author(s):  
Tatsuo Atsumi ◽  
Eriko Fujimoto ◽  
Masakazu Furuta ◽  
Mikio Kato
Keyword(s):  
Escherichia Coli ◽  
Gamma Rays ◽  
Swimming Speed ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Dependent Manner ◽  
Survival Ratio ◽  
Cellular Machinery ◽  
Cellular Dna ◽  
Dose Dependent

AbstractThe effects of ionizing radiation on bacteria are generally evaluated from the dose-dependent survival ratio, which is determined by colony-forming ability and mutation rate. The mutagenic damage to cellular DNA induced by radiation has been extensively investigated; however, the effects of irradiation on the cellular machinery in situ remain unclear. In the present work, we irradiated Escherichia coli cells in liquid media with gamma rays from 60Co (in doses up to 8 kGy). The swimming speeds of the cells were measured using a microscope. We found that the swimming speed was unaltered in cells irradiated with a lethal dose of gamma rays. However, the fraction of motile cells decreased in a dose-dependent manner. Similar results were observed when protein synthesis was inhibited by treatment with kanamycin. Evaluation of bacterial swimming speed and the motile fraction after irradiation revealed that some E. coli cells without the potential of cell growth and division remained motile for several hours after irradiation.

scholarly journals Physico-Chemical Analysis of Mango Juice Factory Efϑluent (MJFE) and its phytotoxic effects of Gingelly (Sesamum indicum L.)

2020 ◽  
Vol 11 (3) ◽  
pp. 4872-4878
Author(s):  
Sivakumar V P ◽  
Lenin M
Keyword(s):  
Agricultural Land ◽  
Growth Parameters ◽  
Dry Weight ◽  
Germination Percentage ◽  
Physicochemical Analysis ◽  
Sesamum Indicum ◽  
Tolerance Index ◽  
Phytotoxic Effects ◽  
Vigour Index ◽  
Mango Juice

The use of bottled fruit beverages has increased enormously. The increased consumption increases the public and sewerage industries. It also leads to various sewerage industries problems. The juice factories use large quantities of water which in turn produces large amounts of wastewater that is called ‘effluent’. This effluent contains a large amount of chemicals and organisms from fruits, sugars and additives like colours. The classical juice factory generates 10 litres of ‘effluent’ per litre of juice. Anextensive range of mangoes are used to make juice; the large quantity of effluent is discharged into nearby water bodies or open agricultural land. The researchers are working on the solution to avoid this. One of the researches we tried to solve the problem of using dilution methods was a physicochemical analysis of mango juice effluent and its phytotoxic effects on gingelly. The laboratory experiment was carried outon the phytotoxic effects of mango juice factory effluent (MJFE)on the growth of Gingelly (Sesamum indicum L.). The different concentrations of effluent(Control, 5, 25, 50, 75and 100%) were prepared, after the seeds sowing the mud pots the treatments were given, the germination percentage(G.P.), vigour index (VI), tolerance index (T.I.), phytotoxicity (P) were recorded in 7thDAS. The growth parameters such as shoot length (S.L.), root length (R.L.), number of leaves (N.L.), total leaf area (TLA), fresh weight (F.W.) and dry weight (D.W.) were inspected on 30th DAS of seedlings.The results were exposed after germination characters, and morphological parameters increased (up to5%) effluent concentration and after that declined in higher (above 25%) concentration of MJFE. Because the effluent is toxic, it contains large quantities of organic and inorganic chemicals and high intensity of BOD, COD and others.

scholarly journals Comparative study of radio-sensitivity and relative biological effectiveness of gamma rays, x-rays, electron beam and proton beam in short grain aromatic rice

2020 ◽  
Vol 80 (04) ◽  
Author(s):  
Deepak Sharma ◽  
Richa Sao ◽  
Parmeshwar K. Sahu ◽  
Gautam Vishwakarma ◽  
J. P. Nair ◽  
...  
Keyword(s):  
Electron Beam ◽  
Proton Beam ◽  
Gamma Rays ◽  
Relative Biological Effectiveness ◽  
Germination Percentage ◽  
Mutation Breeding ◽  
X Rays ◽  
X Ray ◽  
Biological Effectiveness ◽  
Radio Sensitivity

Knowledge about the type of mutagen used and its optimized dose are of paramount importance to design and implement any plant mutation breeding programme. Present study was first time carried out to evaluate the comparative effectiveness, radio-sensitivity behavior and relative biological effectiveness of four physical mutagens viz., gamma rays, X-rays, electron beam and proton beam on two short grain aromatic rice landraces viz., Samundchini and Vishnubhog. The seeds of these two varieties were treated with 15 different doses of all four mutagens, ranging from 50Gy to 750Gy with an interval of 50Gy. Germination percentage and seedling growth parameters were recorded at seven and 15 days after sowing, respectively in two replications. It was observed that germination percentage, shoot and root length of the seedling gradually declined with the increase in doses of all the physical mutagens. On the basis of these observations, LD50 and GR50 doses were calculated. The present study reports the optimum range of doses for gamma ray (280 to 350 Gy); electron beam (290 to 330Gy); X-ray (200 to 250 Gy) and proton beam (150 to 200Gy). GR50 doses were observed higher than LD50 doses for all the mutagens in both landraces. However, Samundchini showed higher LD50 and GR50 doses than Vishnubhog indicating later to be more radio-sensitive. Furthermore, both the genotypes were highly radio-sensitive for proton beam and least for gamma rays. Similarly, high relative biological effectiveness was observed for proton beam followed by X-ray, electron beam and gamma rays indicating their decreasing trend of penetration capacity and lethality. Results of present study will be useful for plant breeders to use the above mutagens in an appropriate dose for mutation breeding in rice.

Sign in / Sign up

Export Citation Format

Share Document