scholarly journals A new design of an electrospinning apparatus for tissue engineering applications

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Juliana R. Dias ◽  
Cyril Dos Santos ◽  
João Horta ◽  
Pedro Lopes Granja ◽  
Paulo Jorge Da Silva Bartolo
Keyword(s):  
Tissue Engineering ◽  
Simple Technique ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Wound Dressings ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Spinning Process ◽  
The Stability

The electrospinning technique is being widely explored in the biomedical field due to its simplicity to produce meshes and its capacity to mimic the micro-nanostructure of the natural extracellular matrix. For skin tissue engineering applications, wound dressings made from electrospun nanofibers present several advantages compared to conventional dressings, such as the promotion of the hemostasis phase, wound exudate absorption, semi-permeability, easy conformability to the wound, functional ability and no scar induction. Despite being a relatively simple technique, electrospinning is strongly influenced by polymer solution characteristics, processing parameters and environmental conditions, which strongly determine the production of fibers and their morphology. However, most electrospinning systems are wrongly designed, presenting a large number of conductive components that compromises the stability of the spinning process. This paper presents a new design of an electrospinning system solving the abovementioned limitations. The system was assessed through the production of polycaprolactone (PCL) and gelatin nanofibers.  Different solvents and processing parameters were considered. Results show that the proposed electrospinning system is suitable to produce reproducible and homogeneous electrospun fibers for tissue engineering applications.

Polycaprolactone(PCL)/Gelati(Ge)-Based Electrospun Nanofibers for Tissue Engineering and Drug Delivery Application

2014 ◽  
Vol 554 ◽  
pp. 57-61 ◽  
Author(s):  
Lor Huai Chong ◽  
Mim Mim Lim ◽  
Naznin Sultana
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Natural Polymer ◽  
Electrospinning Technique ◽  
Minimum Concentration ◽  
Promising Alternative ◽  
Injured Tissue ◽  
Biomaterial Scaffold

Recent development of tissue engineering has been emphasized on tissue regeneration and repairing in order to solve the limitation of organ and tissue transplantation issues. Biomaterial scaffold, which plays an important role in this development, not only provides a promising alternative in order to improve the efficiency of cell transplantation in tissue engineering but also to deliver cells with growth factors and drugs into injured tissue to increase the survival of cell via drug delivery system. In this study, nanofibers were fabricated through blending of a synthetic polymer polycaprolactone (PCL) and a natural polymer Gelatin (Ge) using electrospinning technique. Processing parameters were optimized to determine the most suitable properties of PCL/Ge nanofibers. The surface morphology of PCL/Ge nanofibers were then characterized using Scanning Electron Microscopy (SEM). Six samples of nanofibers from different amount of gelatin mixed with 10% PCL (w/v) were successfully fabricated. Experimental results showed that 18kV of high voltage provided more homogenous and less beaded nanofibers. Meanwhile, the 0.8g of Ge in 10% PCL (w/v) was set as the maximum concentration while 0.2g of Ge in 10% PCL (w/v) was set as the minimum concentration to reduce the bead formation.

scholarly journals In VitroBiological Evaluation of Electrospun Polycaprolactone/Gelatine Nanofibrous Scaffold for Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Mim Mim Lim ◽  
Tao Sun ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Fibre Diameter ◽  
Nanofibrous Scaffold ◽  
Skin Tissue ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Cell Culture Study ◽  
Skin Tissue Engineering

The fabrication of biocompatible and biodegradable scaffolds which mimic the native extracellular matrix of tissues to promote cell adhesion and growth is emphasized recently. Many polymers have been utilized in scaffold fabrication, but there is still a need to fabricate hydrophilic nanosized fibrous scaffolds with an appropriate degradation rate for skin tissue engineering applications. In this study, nanofibrous scaffolds of a biodegradable synthetic polymer, polycaprolactone (PCL), and blends of PCL with a natural polymer, gelatine (Ge), in three different compositions: 85 : 15, 70 : 30, and 50 : 50 were fabricated via an electrospinning technique. The nanofibrous scaffold prepared from 14% w/v PCL/Ge (70 : 30) exhibited more balanced properties of homogeneous nanofibres with an average fibre diameter of 155.60 ± 41.13 nm, 83% porosity, and surface roughness of 176.27 ± 2.53 nm.In vitrocell culture study using human skin fibroblasts (HSF) demonstrated improved cell attachment with a flattened morphology on the PCL/Ge (70 : 30) nanofibrous scaffold and accelerated proliferation on day 3 compared to the PCL nanofibrous scaffold. These results show that the PCL/Ge (70 : 30) nanofibrous scaffold was more favourable and has the potential to be a promising scaffold for skin tissue engineering applications.

New Wound-Dressing Coaxial Electrospun Nanofibers Dressing

2013 ◽  
Vol 790 ◽  
pp. 570-574
Author(s):  
Feng Cheng ◽  
Jing Gao ◽  
Lu Wang ◽  
Yu Hua Yao ◽  
Chun Ling Zeng
Keyword(s):  
Magnetic Particles ◽  
Active Agent ◽  
Electrospun Nanofibers ◽  
Wound Dressings ◽  
Electrospinning Technique ◽  
Spinning Process ◽  
One Step ◽  
The Common ◽  
And Control ◽  
Made In

Coaxial electrospinning technique was developed on the basis of electrospinning. Despite the common advantages of electro-spun, it can also protect the additives which were covered in the nuclear of the yarn during the spinning process and control the release of these additives, such as drugs, proteins, magnetic particles and the active agent, moreover it can be made in one-step. These make Coaxial electro-spun nanofibers have a broad application prospects in the field of wound dressings and drug release. This paper is to describe the unique applications of coaxial electro-spinning in wound healing and the further research of this technology.

Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

2018 ◽  
Author(s):  
Antonios Keirouz ◽  
Giuseppino Fortunato ◽  
Anthony Callanan ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Tensile Modulus ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Skin Substitute ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Needleless Electrospinning ◽  
High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

CELL LADEN ALGINATE/ALBUMIN HYDROGEL FIBERS FOR POTENTIAL SKIN TISSUE ENGINEERING APPLICATIONS

2018 ◽  
Vol 30 (06) ◽  
pp. 1850045
Author(s):  
Maria Grazia Cascone ◽  
Elisabetta Rosellini ◽  
Simona Maltinti ◽  
Andrea Baldassare ◽  
Luigi Lazzeri
Keyword(s):  
Tissue Engineering ◽  
A549 Cells ◽  
Average Diameter ◽  
Favorable Effect ◽  
Engineering Applications ◽  
Residual Amount ◽  
Spinning Process ◽  
Proliferation Ability ◽  
Alginate Fibers ◽  
Kinetics Of

Alginate hydrogel fibers are receiving a great attention for tissue engineering applications. However, an important limitation of alginate is that it does not provide cell adhesion motifs. In this work, albumin was blended with alginate to improve the compatibility of alginate fibers with cells. Cell laden alginate/albumin fibers, potentially usable for skin regeneration, were obtained through a spinning process, by extruding an alginate/albumin solution containing cells into a calcium chloride solution. Cell laden pure alginate fibers were prepared for comparison. Plain alginate and alginate/albumin fibers were also produced. Morphological, mechanical and functional properties of the produced fibers were investigated. In addition, the ability of the fibers to release albumin and to support the viability and growth of A549 cells embedded into them was studied. Fibers with a uniform shape and an average diameter within the range 550–570[Formula: see text][Formula: see text]m were produced. The water content was [Formula: see text]% for alginate fibers, and [Formula: see text]% for alginate/albumin fibers. Stress–strain tests showed, up to a strain value of 20%, the same Young’s modulus for the produced fibers, regardless of the presence of albumin. Overall, obtained results demonstrated that morphology, size, hydrophilicity and mechanical properties were not affected by albumin. Albumin was gradually released over a period of 4 days, with a residual amount (13%) remaining into the fibers. Viability test was carried out on A549 cells, laden inside alginate and alginate/albumin fibers, to evaluate cell proliferation ability. A favorable effect of albumin on the loaded cells was evidenced by a faster kinetics of growth.

Gamma radiation-induced grafting of n-hydroxyethyl acrylamide onto poly(3-hydroxybutyrate): A companion study on its polyurethane scaffolds meant for potential skin tissue engineering applications

2020 ◽  
Vol 116 ◽  
pp. 111176
Author(s):  
Eric Ivan Ochoa-Segundo ◽  
Maykel González-Torres ◽  
Alejandro Cabrera-Wrooman ◽  
Roberto Sánchez-Sánchez ◽  
Blanca Margarita Huerta-Martínez ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gamma Radiation ◽  
Skin Tissue ◽  
Engineering Applications ◽  
Skin Tissue Engineering ◽  
Radiation Induced ◽  
Radiation Induced Grafting

Effects of Process Parameters on Formation of Hybrid Tissue Constructs

2013 ◽  
Author(s):  
Karen Chang Yan ◽  
Pamela Hitscherich ◽  
James Ferrie
Keyword(s):  
Tissue Engineering ◽  
Processing Parameters ◽  
Living Cells ◽  
Live Cells ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Tissue Constructs ◽  
Key Issues ◽  
Working Distance

Tissue engineering is a promising aspect of regenerative medicine that is aimed at constructing functional tissues and organs. While progresses in tissue engineering have led successful clinic applications, challenges remain for more complex tissues/organs that require concerted efforts from multiple types of cells. One of the key issues in building replacements for complex tissues/organs is to mimic the organ’s complex natural organization using a mixture of engineered materials and living cells [1]. Electrospinning has shown promise as a technique to create the microenvironment necessary for cell growth and proliferation for tissue engineering applications[2–4], while multiple fabrication methods have been developed to manipulate live cells(e.g. cell printing) [5–7]. To this end, a system integrating polymer electrospinning technique and pressure-driven cell deposition method is currently under development for forming hybrid tissue constructs with living cells and polymers. This study focuses on examining morphology of electrospun fibers as function of processing parameters including working distance and solution flow rate.

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