Effects of Process Parameters on Formation of Hybrid Tissue Constructs

2013 ◽  
Author(s):  
Karen Chang Yan ◽  
Pamela Hitscherich ◽  
James Ferrie
Keyword(s):  
Tissue Engineering ◽  
Processing Parameters ◽  
Living Cells ◽  
Live Cells ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Tissue Constructs ◽  
Key Issues ◽  
Working Distance

Tissue engineering is a promising aspect of regenerative medicine that is aimed at constructing functional tissues and organs. While progresses in tissue engineering have led successful clinic applications, challenges remain for more complex tissues/organs that require concerted efforts from multiple types of cells. One of the key issues in building replacements for complex tissues/organs is to mimic the organ’s complex natural organization using a mixture of engineered materials and living cells [1]. Electrospinning has shown promise as a technique to create the microenvironment necessary for cell growth and proliferation for tissue engineering applications[2–4], while multiple fabrication methods have been developed to manipulate live cells(e.g. cell printing) [5–7]. To this end, a system integrating polymer electrospinning technique and pressure-driven cell deposition method is currently under development for forming hybrid tissue constructs with living cells and polymers. This study focuses on examining morphology of electrospun fibers as function of processing parameters including working distance and solution flow rate.

The new generation of the biologicalengineering materials for applications in medical and dental implant-scaffolds

2018 ◽  
Vol 2 (91) ◽  
pp. 56-85 ◽  
Author(s):  
L.A. Dobrzański ◽  
A.D. Dobrzańska-Danikiewicz ◽  
Z.P. Czuba ◽  
L.B. Dobrzański ◽  
A. Achtelik-Franczak ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Dental Implant ◽  
Surface Engineering ◽  
Sol Gel ◽  
Dip Coating ◽  
Living Cells ◽  
Ti6al4v Alloy ◽  
Live Cells ◽  
Biological Engineering ◽  
Processing Technologies

Purpose: The publication aims to find the relationship between the proliferation of surface layers of living cells and the deposition of thin atomic layers deposition ALD coatings on the pores internal surfaces of porous skeletons of medical and dental implant-scaffolds manufactured with the selective laser deposition SLS additive technology using titanium and Ti6Al4V alloy. Design/methodology/approach: The extensive review of the literature presents the state-of-the-art in the field of regenerative medicine and tissue engineering. General ageing of societies, increasing the incidence of oncological diseases and some transport and sports accidents, and also the spread of tooth decay and tooth cavities in many regions of the world has taken place nowadays. Those reasons involve resection of many tissues and organs and the need to replace cavities, among others bones and teeth through implantation, more and more often hybridized with tissue engineering methods. Findings: The results of investigations of the structure and properties of skeleton microporous materials produced from titanium and Ti6Al4V alloy powders by the method of selective laser sintering have been presented. Particularly valuable are the original and previously unpublished results of structural research using high-resolution transmission electron microscope HRTEM. Particular attention has been paid to the issues of surface engineering, in particular, the application of flat TiO2 and Al2O3 coatings applied inside micropores using the atomic layers deposition ALD method and hydroxyapatite applied the dip-coating sol-gel method, including advanced HRTEM research. The most important part of the work concerns the research of nesting and proliferation of live cells of osteoblasts the hFOB 1.19 (Human ATCC - CRL - 11372) culture line on the surface of micropores with surfaces covered with the mentioned layers. Research limitations/implications: The investigations reported in the paper fully confirmed the idea of the hybrid technology of producing microporous implants and implant-scaffolds to achieve original Authors’ biological-engineering materials. The surface engineering issues, including both flat-layered nonorganic coatings and interactions of those coverings with flat layers of living cells, play a crucial role. Originality/value: Materials commonly used in implantology and the most commonly used materials processing technologies in those applications have been described. Against that background, the original Authors' concept of implant-scaffolds and the application of microporous skeleton materials for this purpose have been presented.

Polycaprolactone(PCL)/Gelati(Ge)-Based Electrospun Nanofibers for Tissue Engineering and Drug Delivery Application

2014 ◽  
Vol 554 ◽  
pp. 57-61 ◽  
Author(s):  
Lor Huai Chong ◽  
Mim Mim Lim ◽  
Naznin Sultana
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Natural Polymer ◽  
Electrospinning Technique ◽  
Minimum Concentration ◽  
Promising Alternative ◽  
Injured Tissue ◽  
Biomaterial Scaffold

Recent development of tissue engineering has been emphasized on tissue regeneration and repairing in order to solve the limitation of organ and tissue transplantation issues. Biomaterial scaffold, which plays an important role in this development, not only provides a promising alternative in order to improve the efficiency of cell transplantation in tissue engineering but also to deliver cells with growth factors and drugs into injured tissue to increase the survival of cell via drug delivery system. In this study, nanofibers were fabricated through blending of a synthetic polymer polycaprolactone (PCL) and a natural polymer Gelatin (Ge) using electrospinning technique. Processing parameters were optimized to determine the most suitable properties of PCL/Ge nanofibers. The surface morphology of PCL/Ge nanofibers were then characterized using Scanning Electron Microscopy (SEM). Six samples of nanofibers from different amount of gelatin mixed with 10% PCL (w/v) were successfully fabricated. Experimental results showed that 18kV of high voltage provided more homogenous and less beaded nanofibers. Meanwhile, the 0.8g of Ge in 10% PCL (w/v) was set as the maximum concentration while 0.2g of Ge in 10% PCL (w/v) was set as the minimum concentration to reduce the bead formation.

Effects of Processing Parameters on the Morphology and Size of Electrospun PHBV Micro- and Nano-Fibers

2007 ◽  
Vol 334-335 ◽  
pp. 1233-1236 ◽  
Author(s):  
Ho Wang Tong ◽  
Min Wang
Keyword(s):  
Tissue Engineering ◽  
Polymer Solution ◽  
Polymer Concentration ◽  
Solution Concentration ◽  
Processing Parameters ◽  
Electrospun Fibers ◽  
Effective Technique ◽  
Electrospinning Technique ◽  
Needle Diameter ◽  
Short Time

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was used to fabricate micro- and nano-fibrous, non-woven mats by electrospinning for potential tissue engineering applications. The morphology and size of electrospun fibers were assessed systematically by varying the processing parameters. It was found that the diameter of the fibers produced generally increased with electrospinning voltage, needle diameter for the polymer jet and polymer solution concentration. Beaded fibers were readily produced at low PHBV concentrations, whereas the needle was blocked within a very short time during electrospinning when the PHBV concentration was too high. At the polymer concentration of 7.5 % w/v, it was shown that beadless PHBV fibers could be generated continuously by adjusting the electrospinning parameters to appropriate values. This study has clearly demonstrated that electrospinning can be an effective technique to produce PHBV micro- and nano-fibers. It has also been shown that composite fibers containing hydroxyapatite (HA) can be produced using the electrospinning technique.

scholarly journals A new design of an electrospinning apparatus for tissue engineering applications

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Juliana R. Dias ◽  
Cyril Dos Santos ◽  
João Horta ◽  
Pedro Lopes Granja ◽  
Paulo Jorge Da Silva Bartolo
Keyword(s):  
Tissue Engineering ◽  
Simple Technique ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Wound Dressings ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Spinning Process ◽  
The Stability

The electrospinning technique is being widely explored in the biomedical field due to its simplicity to produce meshes and its capacity to mimic the micro-nanostructure of the natural extracellular matrix. For skin tissue engineering applications, wound dressings made from electrospun nanofibers present several advantages compared to conventional dressings, such as the promotion of the hemostasis phase, wound exudate absorption, semi-permeability, easy conformability to the wound, functional ability and no scar induction. Despite being a relatively simple technique, electrospinning is strongly influenced by polymer solution characteristics, processing parameters and environmental conditions, which strongly determine the production of fibers and their morphology. However, most electrospinning systems are wrongly designed, presenting a large number of conductive components that compromises the stability of the spinning process. This paper presents a new design of an electrospinning system solving the abovementioned limitations. The system was assessed through the production of polycaprolactone (PCL) and gelatin nanofibers.  Different solvents and processing parameters were considered. Results show that the proposed electrospinning system is suitable to produce reproducible and homogeneous electrospun fibers for tissue engineering applications.

The Effect of Processing Parameters Onpoly (Lactic Acid)/Poly(Ethylene Oxide)Bicomponentultrafine Fibersby Co-Electrospinning

2013 ◽  
Vol 701 ◽  
pp. 254-258 ◽  
Author(s):  
Suttipan Pavasupree ◽  
Kawee Srikulkit ◽  
Ratthapol Rangkupan
Keyword(s):  
Lactic Acid ◽  
Flow Rate ◽  
Applied Voltage ◽  
Water Soluble ◽  
Flow Rate Ratio ◽  
Poly Lactic Acid ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Water Soluble Polymer

Poly (lactic acid) (PLA)/polyethylene oxide (PEO) bicomponent fibers werefabricated by co-electrospinning technique in a side by side configuration. Effect of PEO concentration, PLA and PEO solution flow rate and an applied voltage on formation, size and morphology of the fibers were investigated. The results showed that the fibers size increased with increasing PEO concentration, PEO flow rate ratio and applied voltage. The composition of the fibers was confirmed by IR spectrum. Additionally, by pairing PEO, which is a water soluble polymer, with PLA, follow by PEO phase removal in water, a C-shaped ultrafine fiber was prepared.

Fabrication and Stabilization of Poly(Acrylonitrile-сo-Methyl Acrylate) Nanofibers

2018 ◽  
Vol 775 ◽  
pp. 43-49
Author(s):  
Krittiya Singcharoen ◽  
Wansika Sirimongkol ◽  
Soontree Khuntong ◽  
Ratthapol Rangkupan
Keyword(s):  
Methyl Acrylate ◽  
Solution Concentration ◽  
Processing Parameters ◽  
Solution Flow Rate ◽  
Fiber Morphology ◽  
Scanning Calorimetry ◽  
Stabilization Time ◽  
Sem Images ◽  
Solution Flow ◽  
Poly Acrylonitrile

In present study, poly (acrylonitrile-co-methyl acrylate) nanofibers were fabricated via electrospinning method and stabilized at elevated temperature in air. Electrospinning processing parameters i.e. solution concentration, solution flow rate and applied voltage were optimized. Fiber morphology and polydispersity index of fiber size was assessed from scanning electron microscope (SEM) images. Selected nanofiber was then used to study effect of stabilization time and stabilization temperature on fiber morphology, change in chemical structure and aromatization index (AI) using Fourier transform infrared spectroscopy and differential scanning calorimetry. SEM images showed drastic morphological change of stabilized fibers compared to the as spun precursor. AI value increased as stabilization time and temperature increased and reaching maximum value of 98%. This indicated high cyclization of the aromatic ring in fiber structure. Current finding is critical for carbonization process and preparation of carbon nanofibers from PAN copolymer in the future.

A REVIEW OF ASSEMBLED POLYACRYLONITRILE-BASED CARBON NANOFIBER PREPARED ELECTROSPINNING PROCESS

2011 ◽  
Vol 10 (03) ◽  
pp. 455-469 ◽  
Author(s):  
A. MATARAM ◽  
A. F. ISMAIL ◽  
M. S. Abdullah ◽  
B. C. Ng ◽  
T. MATSUURA
Keyword(s):  
Polymer Solution ◽  
Flow Rate ◽  
Carbon Nanofiber ◽  
Electrical Potential ◽  
Polymer Melt ◽  
Processing Parameters ◽  
Electrospinning Process ◽  
Solution Flow Rate ◽  
Solution Flow ◽  
Wide Range

Electrospinning is a very simple and versatile process by which polymer nanofibers with diameters ranging from a few nanometers to several micrometers can be produced using an electrostatically driven jet of polymer solution (or polymer melt). Significant progress has been made in this process throughout the last decade and the resultant nanostructures have been exploited to a wide range of applications. An important feature of the electrospinning process is that electrospinning nanofibers are produced in atmospheric air and at room temperature. This paper reviews the assembled polyacrylonitrile (PAN)-based carbon nanofibers with various processing parameters such as electrical potential, distance between capillary and collector drum, solution flow rate (dope extrusion rate), and concentration of polymer solution. The average fiber diameter would increase with increasing concentration of the polymer solution and the flow rate. Therefore, the screen distance could also increase but the average electrical potential of the fibers diameter decreases. Electrospinning process can be conducted at higher electrical potentials, lower flow rate, nearer screen distance, and higher concentrations of dope.

scholarly journals FABRICATION OF CURCUMIN LOADED NANO POLYCAPROLACTONE/CHITOSAN NONWOVEN FABRIC VIA ELECTROSPINNING TECHNIQUE

2018 ◽  
Vol 55 (1B) ◽  
pp. 99 ◽  
Author(s):  
Minh Son Hoang
Keyword(s):  
Electron Microscopy ◽  
Release Kinetics ◽  
Nonwoven Fabric ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Transmission Electron ◽  
In Vitro Technique ◽  
Kinetics Of

Cucurmin loaded poly e–caprolactone/chitosan (PCL/CTS) nanoscale nonwoven fabric was successfully fabricated using electrospinning, a facial and efficient method. The surface tensions of PCL/CTS blend solutions in various ratios were measured to evaluate the influence of PCL/CTS ratio on fibers formation. The effects of process parameter such as the applied voltage, tip–collector distance and the solution flow rate on the fiber generation and morphology of final fiber were optimized. Nanofibers morphology and structure were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. The prepared fibers had a smooth surface and fine morphology. The diameter of fibers ranges from 200  nm to 400 nm. The release kinetics of curcumin loaded samples were also analyzed via in vitro technique. Results demonstrated that the polycaprolactone/chitosan–based nanofibers encapsulate curcumin is a potential material for wound healing acceleration.

scholarly journals Rapid Processing of In-Doped ZnO by Spray Pyrolysis from Environment-Friendly Precursor Solutions

Coatings ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 245 ◽  
Author(s):  
Nina Winkler ◽  
Adhi Wibowo ◽  
Bernhard Kubicek ◽  
Wolfgang Kautek ◽  
Giovanni Ligorio ◽  
...  
Keyword(s):  
Spray Pyrolysis ◽  
Metal Salt ◽  
Low Cost ◽  
Ultrasonic Spray Pyrolysis ◽  
High Growth ◽  
Processing Parameters ◽  
Solution Flow Rate ◽  
Solution Flow ◽  
Ultrasonic Spray ◽  
Rapid Processing

This study focused on the deposition of indium-doped zinc oxide (IZO) films at high growth rates by ultrasonic spray pyrolysis. We investigated the influence of processing parameters, such as temperature and solution flow rate, on the structural, optical, and electrical film properties. For all depositions, low-cost and low-toxicity aqueous solutions and metal salt precursors were used. Through the optimization of the spraying parameters and pattern, a spatially homogeneous IZO layer with transparency greater than 80%, resistivity of 3.82 × 10−3 Ω·cm for a thickness of 1800 nm (sheet resistance of 21.2 Ω/sq), Hall carrier density of 1.36 × 1020 cm−3, Hall mobility of 12.01 cm2 V−1 s−1, and work function of 4.4 eV was obtained. These films are suitable for implementation in optoelectronic and photovoltaic devices.

Optimizing Process Variables to Control Fiber Diameter of Electrospun Polycaprolactone Nanofiber Using Factorial Design

2011 ◽  
Vol 1316 ◽  
Author(s):  
Saida P. Khan ◽  
Kadambari Bhasin ◽  
Golam M. Newaz
Keyword(s):  
Dielectric Constant ◽  
Factorial Design ◽  
Fiber Diameter ◽  
Polymer Concentration ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Solution Flow Rate ◽  
Tissue Engineering Application ◽  
Electrospinning Technique ◽  
Solution Flow

ABSTRACTIn the electrospinning process, fibers ranging from 50 nm to 1000 nm or greater can be produced by applying an electric potential to a polymeric solution [1, 2]. Our group has studied the fabrication of electro-spun Poly-caprolactone (PCL) nanofiber consisting of a range of fiber diameter (nm-um) and pore sizes. PCL is a biocompatible, FDA approved and biodegradable [3, 4] polymer. As a solvent we have used 2,2,2-trifluoroethanol (TFE) for its biocompatibility, conductivity and high dielectric constant. The electrospinning technique consists of a simple setup with a number of variables working in a complex and unpredictable way. The variables affecting fiber diameter are polymer concentration in the solution, flow rate, applied voltage, tip to collector distance, diameter of the needle/capillary, polymer/solvent dielectric constant etc. In our study we have found that concentration of the solution and molecular weight of the polymer are the most important parameters for forming the nanofibers and viscosity is important for the fiber diameter. To optimize so many variables to control the fiber diameter, we have used the factorial design method. The study is important for the fabrication of biomimetic scaffold for vascular implant and tissue engineering application.

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