Solvent-based Extrusion 3D Printing for the Fabrication of Tissue Engineering Scaffolds

Three-dimensional (3D) printing has been emerging as a new technology for scaffold fabrication to overcome the problems associated with the undesirable microstructure associated with the use of traditional methods. Solvent-based extrusion (SBE) 3D printing is a popular 3D printing method, which enables incorporation of cells during the scaffold printing process. The scaffold can be customized by optimizing the scaffold structure, biomaterial, and cells to mimic the properties of natural tissue. However, several technical challenges prevent SBE 3D printing from translation to clinical use, such as the properties of current biomaterials, the difficulties associated with simultaneous control of multiple biomaterials and cells, and the scaffold-to-scaffold variability of current 3D printed scaffolds. In this review paper, a summary of SBE 3D printing for tissue engineering (TE) is provided. The influences of parameters such as ink biomaterials, ink rheological behavior, cross-linking mechanisms, and printing parameters on scaffold fabrication are considered. The printed scaffold structure, mechanical properties, degradation, and biocompatibility of the scaffolds are summarized. It is believed that a better understanding of the scaffold fabrication process and assessment methods can improve the functionality of SBE-manufactured 3D printed scaffolds.

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3D Printing of Conductive Tissue Engineering Scaffolds Containing Polypyrrole Nanoparticles with Different Morphologies and Concentrations

Materials ◽

10.3390/ma12152491 ◽

2019 ◽

Vol 12 (15) ◽

pp. 2491 ◽

Cited By ~ 6

Author(s):

Chunyang Ma ◽

Le Jiang ◽

Yingjin Wang ◽

Fangli Gang ◽

Nan Xu ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Three Dimensional ◽

Solvent Casting ◽

Tissue Engineering Scaffolds ◽

New Era ◽

Conductive Materials ◽

Conductive Film ◽

Traditional Preparation ◽

Natural Tissue

Inspired by electrically active tissues, conductive materials have been extensively developed for electrically active tissue engineering scaffolds. In addition to excellent conductivity, nanocomposite conductive materials can also provide nanoscale structure similar to the natural extracellular microenvironment. Recently, the combination of three-dimensional (3D) printing and nanotechnology has opened up a new era of conductive tissue engineering scaffolds exhibiting optimized properties and multifunctionality. Furthermore, in the case of two-dimensional (2D) conductive film scaffolds such as periosteum, nerve membrane, skin repair, etc., the traditional preparation process, such as solvent casting, produces 2D films with defects of unequal bubbles and thickness frequently. In this study, poly-l-lactide (PLLA) conductive scaffolds incorporated with polypyrrole (PPy) nanoparticles, which have multiscale structure similar to natural tissue, were prepared by combining extrusion-based low-temperature deposition 3D printing with freeze-drying. Furthermore, we creatively integrated the advantages of 3D printing and solvent casting and successfully developed a 2D conductive film scaffold with no bubbles, uniform thickness, and good structural stability. Subsequently, the effects of concentration and morphology of PPy nanoparticles on electrical properties and mechanical properties of 3D conductive scaffolds and 2D conductive films scaffolds have been studied, which provided a new idea for the design of both 2D and 3D electroactive tissue engineering scaffolds.

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Three-Dimensional Printing of Hydroxyapatite Composites for Biomedical Application

Crystals ◽

10.3390/cryst11040353 ◽

2021 ◽

Vol 11 (4) ◽

pp. 353

Author(s):

Yanting Han ◽

Qianqian Wei ◽

Pengbo Chang ◽

Kehui Hu ◽

Oseweuba Valentine Okoro ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Biomedical Application ◽

Three Dimensional ◽

Antibacterial Properties ◽

Natural Polymers ◽

Three Dimensional Printing ◽

3D Printed ◽

Dental Applications ◽

Hard Tissue Engineering

Hydroxyapatite (HA) and HA-based nanocomposites have been recognized as ideal biomaterials in hard tissue engineering because of their compositional similarity to bioapatite. However, the traditional HA-based nanocomposites fabrication techniques still limit the utilization of HA in bone, cartilage, dental, applications, and other fields. In recent years, three-dimensional (3D) printing has been shown to provide a fast, precise, controllable, and scalable fabrication approach for the synthesis of HA-based scaffolds. This review therefore explores available 3D printing technologies for the preparation of porous HA-based nanocomposites. In the present review, different 3D printed HA-based scaffolds composited with natural polymers and/or synthetic polymers are discussed. Furthermore, the desired properties of HA-based composites via 3D printing such as porosity, mechanical properties, biodegradability, and antibacterial properties are extensively explored. Lastly, the applications and the next generation of HA-based nanocomposites for tissue engineering are discussed.

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Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

Biomedical glasses ◽

10.1515/bglass-2020-0006 ◽

2020 ◽

Vol 6 (1) ◽

pp. 57-69

Author(s):

Amirhosein Fathi ◽

Farzad Kermani ◽

Aliasghar Behnamghader ◽

Sara Banijamali ◽

Masoud Mozafari ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Three Dimensional ◽

Layer By Layer ◽

Composite Scaffolds ◽

3D Printed ◽

Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

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Advances in 3D Printing for Tissue Engineering

Materials ◽

10.3390/ma14123149 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3149

Author(s):

Angelika Zaszczyńska ◽

Maryla Moczulska-Heljak ◽

Arkadiusz Gradys ◽

Paweł Sajkiewicz

Keyword(s):

Tissue Engineering ◽

Additive Manufacturing ◽

3D Printing ◽

Complex Structure ◽

Three Dimensional ◽

Computer Assisted ◽

Scaffold Fabrication ◽

Manufacturing Techniques ◽

Printing Techniques ◽

State Of Art

Tissue engineering (TE) scaffolds have enormous significance for the possibility of regeneration of complex tissue structures or even whole organs. Three-dimensional (3D) printing techniques allow fabricating TE scaffolds, having an extremely complex structure, in a repeatable and precise manner. Moreover, they enable the easy application of computer-assisted methods to TE scaffold design. The latest additive manufacturing techniques open up opportunities not otherwise available. This study aimed to summarize the state-of-art field of 3D printing techniques in applications for tissue engineering with a focus on the latest advancements. The following topics are discussed: systematics of the available 3D printing techniques applied for TE scaffold fabrication; overview of 3D printable biomaterials and advancements in 3D-printing-assisted tissue engineering.

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The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

Materials Science Forum ◽

10.4028/www.scientific.net/msf.934.129 ◽

2018 ◽

Vol 934 ◽

pp. 129-133 ◽

Cited By ~ 1

Author(s):

Chao Fan Lv ◽

Li Ya Zhu ◽

Jian Ping Shi ◽

Zong An Li ◽

Wen Lai Tang ◽

...

Keyword(s):

Tissue Engineering ◽

Sodium Alginate ◽

Inkjet Printing ◽

Three Dimensional ◽

3D Bioprinting ◽

Tissue Engineering Scaffolds ◽

Printing Technology ◽

3D Printed ◽

Inkjet Printing Technology ◽

Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

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3D-Printed Biopolymers for Tissue Engineering Application

International Journal of Polymer Science ◽

10.1155/2014/829145 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 50

Author(s):

Xiaoming Li ◽

Rongrong Cui ◽

Lianwen Sun ◽

Katerina E. Aifantis ◽

Yubo Fan ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Three Dimensional ◽

Engineering Application ◽

Tissue Engineering Application ◽

Printing Technology ◽

3D Printing Technology ◽

Potential Applications ◽

3D Printed ◽

Dimensional Object

3D printing technology has recently gained substantial interest for potential applications in tissue engineering due to the ability of making a three-dimensional object of virtually any shape from a digital model. 3D-printed biopolymers, which combine the 3D printing technology and biopolymers, have shown great potential in tissue engineering applications and are receiving significant attention, which has resulted in the development of numerous research programs regarding the material systems which are available for 3D printing. This review focuses on recent advances in the development of biopolymer materials, including natural biopolymer-based materials and synthetic biopolymer-based materials prepared using 3D printing technology, and some future challenges and applications of this technology are discussed.

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Importance of 3D Printing Technology in Medical Fields

Additive Manufacturing Technologies From an Optimization Perspective - Advances in Logistics, Operations, and Management Science ◽

10.4018/978-1-5225-9167-2.ch002 ◽

2019 ◽

pp. 21-40

Author(s):

Ranjit Barua ◽

Sudipto Datta ◽

Amit Roychowdhury ◽

Pallab Datta

Keyword(s):

Tissue Engineering ◽

Additive Manufacturing ◽

3D Printing ◽

New Technology ◽

Three Dimensional ◽

Layer By Layer ◽

Medical Field ◽

Printing Technology ◽

3D Printing Technology ◽

Proper Material

Three-dimensional or 3D printing technology is a growing interest in medical fields like tissue engineering, dental, drug delivery, prosthetics, and implants. It is also known as the additive manufacturing (AM) process because the objects are done by extruding or depositing the material layer by layer, and the material may be like biomaterials, plastics, living cells, or powder ceramics. Specially in the medical field, this new technology has importance rewards in contrast with conventional technologies, such as the capability to fabricate patient-explicit difficult components, desire scaffolds for tissue engineering, and proper material consumption. In this chapter, different types of additive manufacturing (AM) techniques are described that are applied in the medical field, especially in community health and precision medicine.

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Benefits And Limitations Of Three-Dimensional Printing Technology For Ecological Research

10.1101/283895 ◽

2018 ◽

Cited By ~ 1

Author(s):

Jocelyn E. Behm ◽

Brenna R. Waite ◽

S. Tonia Hsieh ◽

Matthew R. Helmus

Keyword(s):

3D Printing ◽

New Technology ◽

Wood Fiber ◽

Three Dimensional ◽

Model Organisms ◽

Successful Implementation ◽

Ecological Research ◽

Predator Attack ◽

Custom Made ◽

3D Printed

AbstractBackgroundEcological research often involves sampling and manipulating non-model organisms that reside in heterogeneous environments. As such, ecologists often adapt techniques and ideas from industry and other scientific fields to design and build equipment, tools, and experimental contraptions custom-made for the ecological systems under study. Three-dimensional (3D) printing provides a way to rapidly produce identical and novel objects that could be used in ecological studies, yet ecologists have been slow to adopt this new technology. Here, we provide ecologists with an introduction to 3D printing.ResultsFirst, we give an overview of the ecological research areas in which 3D printing is predicted to be the most impactful and review current studies that have already used 3D printed objects. We then outline a methodological workflow for integrating 3D printing into an ecological research program and give a detailed example of a successful implementation of our 3D printing workflow for 3D printed models of the brown anole, Anolis sagrei, for a field predation study. After testing two print media in the field, we show that the models printed from the less expensive and more sustainable material (blend of 70% plastic and 30% recycled wood fiber) were just as durable and had equal predator attack rates as the more expensive material (100% virgin plastic).ConclusionsOverall, 3D printing can provide time and cost savings to ecologists, and with recent advances in less toxic, biodegradable, and recyclable print materials, ecologists can choose to minimize social and environmental impacts associated with 3D printing. The main hurdles for implementing 3D printing – availability of resources like printers, scanners, and software, as well as reaching proficiency in using 3D image software – may be easier to overcome at institutions with digital imaging centers run by knowledgeable staff. As with any new technology, the benefits of 3D printing are specific to a particular project, and ecologists must consider the investments of developing usable 3D materials for research versus other methods of generating those materials.

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Recent advances in the development of nature-derived photocrosslinkable biomaterials for 3D printing in tissue engineering

Biomaterials Research ◽

10.1186/s40824-019-0168-8 ◽

2019 ◽

Vol 23 (1) ◽

Cited By ~ 7

Author(s):

Geunho Choi ◽

Hyung Joon Cha

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Uv Light ◽

Three Dimensional ◽

Main Body ◽

Biological Functions ◽

Non Invasive ◽

Decellularized Extracellular Matrix ◽

3D Printed ◽

Control Light

Abstract Background In recent years, three-dimensional (3D) printing has begun to be widely used in tissue engineering. Natural biomaterials have been employed to overcome the limitations of synthetic polymers. However, their low mechanical strength and poor printability are major disadvantages. Photocrosslinking is the most promising fabrication strategy because it is non-invasive and easy to control light intensity and exposure. In this article, developments of photocrosslinkable natural biomaterials in the field of 3D printing are reviewed. Main body Photocrosslinkable biomaterials can be broadly classified into materials that use ultraviolet (UV) and visible lights. Many natural biomaterials such as gelatin, hydroxyapatite, silk fibroin, and pectin have been modified through acrylation, crosslinked by 365 nm UV light, and 3D printed. Riboflavin could also be used to crosslink and print collagen or decellularized extracellular matrix (dECM). In the case of silk-like aneroin and modified gelatin, crosslinking is possible by forming a dityrosine bond using 452 nm visible light. Conclusion Despite the tremendous researches on the developments of photocrosslinkable 3D printing natural biomaterials, further efforts are necessary to develop source biomaterials with excellent biological functions and sufficient mechanical integrity.

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