scholarly journals The qPCR analysis of vaginal microflora for the diagnosis of bacterial vaginosis

2019 ◽  
Vol 2 (2) ◽  
pp. e00084
Author(s):  
M.Е. Senina ◽  
Y.А. Savochkina ◽  
L.V. Skvortsov ◽  
T.I. Popova ◽  
L.V. Dzhedzheia
Keyword(s):  
Bacterial Vaginosis ◽  
Molecular Genetic ◽  
Anaerobic Bacteria ◽  
Molecular Genetic Analysis ◽  
Sexually Transmitted ◽  
Vaginal Microflora ◽  
Normal Microflora ◽  
Polymerase Chain ◽  
Tract Infections ◽  
General Balance

The correct information about of the vaginal microflora plays an important role in preventing the occurrence of urinary tract infections and sexually transmitted infections among women. Disbalance of obligate and facultative microflora causes disbacteriosis, a risk factor for emergence of infectious diseases. It is known that the cause of bacterial vaginosis (BV) is not a single pathogen but a impairments in of the general balance of the vaginal microflora, which manifests a decrease of the normal microflora (Lactobacillus spp) and intense increase of pathogenic aerobic and anaerobic bacteria. The development of molecular genetic analysis methods, in particular, approaches based on the use of polymerase chain reaction (PCR), significantly expanded understanding of the diversity of microbial biotopes, including identification of the key and new «players» in the development of BV. The aim of our study was to evaluate the performance of real-time PCR kit «Femoscreen» («Lytech», Russia) for comprehensive BV diagnosis.

scholarly journals Comparison of the Vaginal Cytological and Microbiological Results in the Detection of Normal Microflora of Pregnant

2018 ◽  
Vol 68 (3) ◽  
pp. 307
Author(s):  
E. S. ÖZDEMIR SALCI ◽  
G. GONCAGÜL ◽  
V. İPEK
Keyword(s):  
Bacterial Vaginosis ◽  
Cell Formation ◽  
Comparative Investigation ◽  
Single Type ◽  
Microbiological Analysis ◽  
Cytological Examination ◽  
Vaginal Microflora ◽  
Normal Microflora ◽  
Clue Cell ◽  
Pathogenic Agents

The aim of this study was to carry out a cytological and microbiological comparative investigation of vaginal microflora in pregnant ewes. The subjects for the study comprised of 39 healthy curly fleeced breed ewes (n=39), approximately 3 years old, at 2-4 months of pregnancy. Two vaginal samples were taken for cytological and microbiological examinations from each ewe in a sterile manner. Hemacolor® was used in cytological examination, while microbiological analysis were completed by conventional techniques. In cytological examination, slides were evaluated to detect lactobacilli, other bacteria, “clue cell” formation and presence of neutrophils. Microbiological investigation was carried out to detect possible pathogens. Cytological results compatible with bacterial vaginosis were obtained in 10 cases. Microbiologically, single type bacteria in 27 cases and more than one bacterium in 12 cases were isolated. The most common isolated pathogen was Escherichia coli. Comparing the cytological and microbiological results, 7 out of 27 cases were compatible with the bacterial vaginosis. In 3 cases of bacterial vaginosis non-pathogenic agents were revealed. In conclusion, it was proven that utilising the cytological examination provides more reliable results for detection of normal vaginal microflora of pregnant ewes.

Central neurocytoma: morphological, flow cytometric, polymerase chain reaction, fluorescence in situ hybridization, and karyotypic analyses

1999 ◽  
Vol 90 (2) ◽  
pp. 348-354 ◽  
Author(s):  
Venita Jay ◽  
Vern Edwards ◽  
Eelco Hoving ◽  
James Rutka ◽  
Laurence Becker ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Ganglion Cells ◽  
Molecular Genetic ◽  
Central Neurocytoma ◽  
Molecular Genetic Analysis ◽  
Fish Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

✓ The results of cytogenetic and molecular genetic analysis of a central neurocytoma are presented. Central neurocytomas are intriguing neoplasms that exhibit primarily neuronal, but also glial characteristics, which indicate an origin from a pluripotential neuroglial precursor. The authors describe an intraventricular neurocytoma in an 11-year-old boy that showed anaplastic features with widespread necrosis and mitoses, as well as extensive calcification and foci that exhibited marked neuronal differentiation with clusters of ganglion cells. Immunohistochemical examination showed prominent synaptophysin and neurofilament positivity and focal glial fibrillary acidic protein positivity. Electron microscopy revealed abundant neuritic processes with microtubules and dense core granules as well as mature ganglion cells. Flow cytometry studies revealed increased S (7.8%) and G2M (9.7%) phase components. Molecular and cytogenetic studies were undertaken to assess whether there were similarities to two other tumor types that exhibit neuronal differentiation, the neuroblastoma and medulloblastoma. Polymerase chain reaction and fluorescence in situ hybridization (FISH) analysis revealed no evidence of amplification of the MYCN oncogene or chromosome 1p deletion, which are common in neuroblastomas. Chromosomal analysis by G banding revealed a complex karyotype, with counts in the near-diploidy range (45–48). Two chromosomes 1 appeared normal on G banding and FISH analysis, with p58 signals present on the distal p arm of both chromosomes 1; however, three additional copies of distal 1q were present in rearrangements with 4 and 7. Although the histological findings indicate a kinship to the neuroblastoma and medulloblastoma, the central neurocytoma appears to have a different karyotypic profile, although more cases need to be assessed using molecular genetic analysis.

The Ikaros gene, a central regulator of lymphoid differentiation, fuses to the BCL6 gene as a result of t(3;7)(q27;p12) translocation in a patient with diffuse large B-cell lymphoma

Blood ◽  
2000 ◽  
Vol 95 (8) ◽  
pp. 2719-2721 ◽  
Author(s):  
Yoshitaka Hosokawa ◽  
Yumiko Maeda ◽  
Ryo Ichinohasama ◽  
Ikuo Miura ◽  
Masafumi Taniwaki ◽  
...  
Keyword(s):  
B Cell ◽  
Molecular Genetic ◽  
Regulatory Region ◽  
B Cell Lymphoma ◽  
Molecular Genetic Analysis ◽  
B Cell Differentiation ◽  
Polymerase Chain ◽  
Large B Cell

The BCL6 gene, isolated from the breakpoints of 3q27-associated chromosomal translocations, has been implicated in diffuse large B-cell lymphomas (DLBL). Here we describe the molecular characterization of novel t(3;7)(q27;p12) translocations in 2 patients with DLBL. Molecular genetic analysis of the breakpoint area involving BCL6 revealed the presence of the Ikaros gene, a central regulator of lymphoid differentiation that had been mapped to human chromosome 7 band p13-p11.1. As a molecular consequence of the translocation, the 5′ regulatory region of the BCL6 gene was replaced by the putative 5′ regulatory region of theIkaros gene, probably leading to deregulated expression of theBCL6 gene throughout B-cell differentiation. Reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH) analyses of a patient sample established that the t(3;7)(q27;p12) results in fusion of the Ikaros andBCL6 genes. This study provides the first evidence that the Ikaros gene is rearranged in human hematopoietic malignant disorders.

scholarly journals Chickpea genotypes characteristics on resistance to fusarium Fusarium oxysporum f. sp. ciceris

2018 ◽  
Vol 23 ◽  
pp. 166-169
Author(s):  
V. A. Chekalov ◽  
N. E. Volkova
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Markers ◽  
Fusarium Oxysporum ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Chain Reaction ◽  
Resistance Level ◽  
Extraction And Purification ◽  
Polymerase Chain ◽  
Southern Ukraine

Aim. Molecular-genetic analysis of the chickpea genotypes for foc0, foc3, foc4 resistance genes to Fusarium oxysporum f. sp ciceris. Methods. Extraction and purification of DNA, spectrophotometry, polymerase chain reaction, electrophoresis in polyacrylamide gels. Results. 35 chickpea lines and varieties of Ukrainian and foreign breeding characterized according to genotyping on foc0, foc3, foc4 genes of resistance to Fusarium oxysporum f. sp ciceris by the microsatellite markers TA59, TR19 and TR59. Fragments of the expected size for all markers were obtained for samples, for which the resistance level was fixed to certain races. Match between data on the presence of a amplification fragment of a certain size and resistance level among other samples is not found. Conclusions. For 35 chickpea varieties and lines the allele state of foc0, foc3, foc4 genes of resistance to the F. oxysporum f. sp ciceris races 0, 3, 4 is established. The variety ‘Pam’yat’ is recommended as a control of resistance to F. oxysporum f. sp ciceris races 0, 3, 4 in the southern Ukraine conditions. Keywords: chickpea, genes, molecular markers, fusarios, resistance.

scholarly journals Bacterial vaginosis.

1991 ◽  
Vol 4 (4) ◽  
pp. 485-502 ◽  
Author(s):  
C A Spiegel
Keyword(s):  
Urinary Tract ◽  
Bacterial Vaginosis ◽  
Urinary Tract Infections ◽  
Anaerobic Bacteria ◽  
Fetal Loss ◽  
Reproductive Age ◽  
Genital Tract Infection ◽  
Increased Risk ◽  
Tract Infections ◽  
Lactobacillus Spp

Bacterial vaginosis (BV) is the most common of the vaginitides affecting women of reproductive age. It appears to be due to an alteration in the vaginal ecology by which Lactobacillus spp., the predominant organisms in the healthy vagina, are replaced by a mixed flora including Prevotella bivia, Prevotella disiens, Porphyromonas spp., Mobiluncus spp., and Peptostreptococcus spp. All of these organisms except Mobiluncus spp. are also members of the endogenous vaginal flora. While evidence from treatment trials does not support the notion that BV is sexually transmitted, recent studies have shown an increased risk associated with multiple sexual partners. It has also been suggested that the pathogenesis of BV may be similar to that of urinary tract infections, with the rectum serving as a reservoir for some BV-associated flora. The organisms associated with BV have also been recognized as agents of female upper genital tract infection, including pelvic inflammatory disease, and the syndrome BV has been associated with adverse outcome of pregnancy, including premature rupture of membranes, chorioamnionitis, and fetal loss; postpartum endometritis; cuff cellulitis; and urinary tract infections. The mechanisms by which the BV-associated flora causes the signs of BV are not well understood, but a role for H2O2-producing Lactobacillus spp. in protecting against colonization by catalase-negative anaerobic bacteria has been recognized. These and other aspects of BV are reviewed.

Histologic Analysis of Gonadal Tissue in Patients with Ullrich-Turner Syndrome and Derivative Y Chromosomes

2005 ◽  
Vol 8 (2) ◽  
pp. 197-203 ◽  
Author(s):  
L.-C. Horn ◽  
A. Limbach ◽  
W. Hoepffner ◽  
R.B. Tröbs ◽  
E. Keller ◽  
...  
Keyword(s):  
At Risk ◽  
Polymerase Chain Reaction ◽  
Turner Syndrome ◽  
Molecular Genetic ◽  
Germ Cell Tumors ◽  
Molecular Genetic Analysis ◽  
Chain Reaction ◽  
Additional Tool ◽  
Polymerase Chain ◽  
Chromosomal Sequences

To identify patients who had Ullrich-Turner syndrome (UTS) and were at risk for gonadoblastoma or associated germ cell tumors, molecular genetic analysis was carried out to detect Y chromosomal sequences. From peripheral blood samples of 5 patients who had cytogenetically confirmed UTS, genomic DNA was extracted and screened for Y chromosomal sequences by polymerase chain reaction. The morphology of the gonadal tissues was compared with results from polymerase chain reaction. Three phenotypic females showed UTS mosaicism with normal X chromosome accompanied by Y chromosomal material, and 2 patients showed marker chromosomes. Molecular analysis represented loci PABY, SRY, ZFY, TSPY, DYZ3, DYZ1 DXYS, 19Y, DYS-273, DYS-148, DYS218, DYS224, and DYZ1. Three patients showed gonadal tumors (1 with unilateral gonadoblastoma, 1 with unilateral dysgerminoma, and 1 patient had both tumors in 1 gonad). Molecular genetic screening for Y chromosomal sequences may be useful as an additional tool for the identification of patients at risk for a gonadal tumor. Careful, complete processing, including step sectioning, of the gonadectomy specimens to detect small lesions is recommended.

scholarly journals Practical aspects of bacterial vaginosis: relevance, diagnosis and comprehensive treatment

2020 ◽  
pp. 61-68
Author(s):  
V.V. Mehedko ◽  
Keyword(s):  
Bacterial Vaginosis ◽  
Antimicrobial Therapy ◽  
Inflammatory Diseases ◽  
Clinical Effectiveness ◽  
Comprehensive Treatment ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Vaginal Microflora ◽  
Local Use ◽  
Prevention Of Relapse

Bacterial vaginosis (BV) is significantly widespread in the population: its detection rate in women with inflammatory diseases of the urinary system is 35%, among pregnant women – 10-30%, among patients with sexually transmitted infection – 20-60%. The presence of BV leads to pregnancy complications, after gynecological surgery and the development of relapses of the disease. To achieve a full therapeutic effect, it is necessary not only to conduct antimicrobial therapy, but also to restore the vaginal biocenosis, which is a condition for preventing relapse. A two-stage treatment for BV was studied using Limenda and Bioselak. The use of the combined antimicrobial drug Limenda at the first stage of treatment of BV showed a high clinical effect (100%) with confirmation of cure according to microscopic examination (97.9%). But after the completion of antimicrobial therapy, only in 29.2% of cases the restoration of the rod vaginal microflora was noted. The local use of the probiotic Bioselak at the second stage of treatment ensured the restoration of the biocenosis in all patients (100%) and a long relapse-free period. The high clinical effectiveness of the proposed scheme allows us to recommend it for the treatment of BV and the prevention of relapse. Key words: bacterial vaginosis, vaginal biocenosis, relapse prevention, local probiotic, Limenda, Bioselak.

scholarly journals ITGA4, ITGB7, TNFα, IL10 genes polymorphisms in the ethnic Buryat patients with ulcerative colitis

2021 ◽  
Vol 49 ◽  
Author(s):  
I. V. Zhilin ◽  
E. Yu. Chashkova ◽  
A. A. Zhilina ◽  
A. Ch. Tsyrempilova
Keyword(s):  
Ulcerative Colitis ◽  
Healthy Volunteers ◽  
Molecular Genetic ◽  
Genetic Material ◽  
Binary Logistic Regression ◽  
Molecular Genetic Analysis ◽  
Binary Logistic Regression Analysis ◽  
Irkutsk Region ◽  
In Trans ◽  
Polymerase Chain

Background: Worldwide studies of genetic material, polymorphisms and prognostic gene models for immune-associated disorders have established differences in trans-ethnic population cohorts, which determine phenotypic and other characteristics of the course of these diseases. Ulcerative colitis (UC) is a  chronic immune inflammation of the colon mucosa. More than 100 gene polymorphisms associated with multiple integrated cross-talks have been discovered.Aim: To study the ITGA4, ITGB7, TNFα, IL10 genes polymorphisms in patients with ulcerative colitis belonging to the Buryat ethnic group and living in Irkutsk region, Buryat Republic and Transbaikal territory.Materials and methods: The study included a total of 49 subjects, 24 of them being UC patients and 25 healthy volunteers, compatible in gender, age and ethnic background. The molecular genetic analysis by real time polymerase chain reaction was performed with DNA samples from whole peripheral blood leucocytes.Results: The differences in the prevalence of the ITGA4(rs1143674, rs1449263), ITGB7(rs11574532), TNFα(rs1800629), and IL10(rs1800871) genotypes were non-significant (р>0.05). The IL10(rs1800896) GG homozygote patients had higher odds ratio (OR) for UC compared to the carriers of other polymorphisms (OR 24; 95%  confidence interval (CI) 2.783–206.969; р=0.001). The AA homozygote type was less frequent among UC patients compared to healthy volunteers (OR 0.17; 95%  CI 0.049–0.589; р=0.004). The analysis of genotype frequency distribution of all studied genes including clinical characteristics of the disease showed no significant results (р>0.05). The binary logistic regression analysis has shown that IL10(rs1800896)GG was an UC predictor with sensitivity of 96% and specificity of 50%  (AUC 0.760; 95% CI 0.621–0.899; p=0.002; standard error 0.71).Conclusion: The GG genotype of IL10(rs1800896) is a  UC predictor, whereas the AA genotype is significantly more prevalent among healthy subjects of the Buryat cohort. 

scholarly journals Microbiological, behavioral and anamnestic predictor factors of bacterial vaginosis in women with vaginal discharge

2016 ◽  
Vol 65 (3) ◽  
pp. 32-42
Author(s):  
Elena V Shipitsyna ◽  
Tatyana A Khusnutdinova ◽  
Olga S Ryzhkova ◽  
Anna A Krysanova ◽  
Olga V Budilovskaya ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Modes Of Transmission ◽  
Vaginal Microflora ◽  
Normal Microflora ◽  
Clinically Significant ◽  
Predictor Factors

Introduction. Bacterial vaginosis (BV) is the primary cause of pathological vaginal discharge in women of reproductive age. Gardnerella vaginalis and Atopobium vaginae are considered key components of the vaginal microflora in BV. Etiology, pathogenesis and modes of transmission of BV are actively studied, however these questions still remain unanswered. Objective: investigate predictor factors of BV in women with vaginal discharge. Material and methods. In total, 318 women were included. As clinical material, vaginal samples were used. BV was diagnosed using the Nugent method. For quantitative determination of G. vaginalis and A. vaginae DNA, real-time PCR was used. Behavioral and anamnestic data were obtained from questionnaire filled out by the patients. Results. BV was diagnosed in 27 % of women. G. vaginalis and A. vaginae DNA was detected, respectively, in 93 % and 83 % of patients with BV, 73 % and 59 % - with intermediate microflora, 52 % and 38 % - with normal microflora. Difference between the three types of microflora in the frequency and concentrations of these microorganisms were statistically significant. Detection of G. vaginalis and A. vaginae were significant predictor factors of BV (OR 12.2; 95 % CI 5.1-29.4 and OR 7.9; 95 % CI 4.2-14.9, respectively), with chances to diagnose BV being manifold increased when clinically significant concentrations of these bacteria were detected (≥3×106 and ≥8×105 DNA copies/ml for G. vaginalis and A. vaginae, respectively). Detection of clue cells in Gram stained preparations was shown to be the strongest BV predictor (OR 765.6; 95 % CI 99.6-5883.2). Conclusions. BV is diagnosed in more than one fourth of women with vaginal discharge. Detection of G. vaginalis and A. vaginae, especially in clinically significant concentrations, and clue cells in Gram stained preparations are significant predictor factors of BV.

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