scholarly journals Defensins – natural peptide antibiotics of higher eucariotes

2014 ◽  
Vol 60 (4) ◽  
pp. 438-447 ◽  
Author(s):  
D.V. Grishin ◽  
N.N. Sokolov
Keyword(s):  
Antimicrobial Peptides ◽  
Biomedical Application ◽  
Structural Features ◽  
General Information ◽  
Current Situation ◽  
Peptide Antibiotics ◽  
Infectious Agents ◽  
First Line ◽  
Natural Peptide ◽  
Main Components

The goal of this review is to characterize defensins representing an evolutionary the most ancient family of antimicrobial peptides. It gives general information on functional and structural features of defensins as the main components of the first-line defense of higher eukaryote organisms against infectious agents. The review considers not only current situation in the defensin research but also perspectives of creation of recombinant antimicrobial peptides of biomedical application.

scholarly journals Expression of Natural Peptide Antibiotics in Human Articular Cartilage and Synovial Membrane

2001 ◽  
Vol 8 (5) ◽  
pp. 1021-1023 ◽  
Author(s):  
Friedrich Paulsen ◽  
Thomas Pufe ◽  
Wolf Petersen ◽  
Bernhard Tillmann
Keyword(s):  
Articular Cartilage ◽  
Antimicrobial Peptides ◽  
Synovial Membrane ◽  
Rare Event ◽  
Joint Inflammation ◽  
Human Articular Cartilage ◽  
Peptide Antibiotics ◽  
Invasive Procedures ◽  
Articular Joint ◽  
Natural Peptide

ABSTRACT In view of frequent present use of invasive procedures on limb joints, it is astonishing that articular joint inflammation is a rare event. We questioned whether antimicrobial peptides play a role in protecting human articular cartilage and synovial membrane against inflammatory agents. Our results implicate defensins in the protection of human articular joints against pathogens.

NATURAL COSMETICS IN THE CARE OF YOUNG CHILDREN

2020 ◽  
Vol 99 (6) ◽  
pp. 155-162
Author(s):  
O.B. Tamrazova ◽  
◽  
S.P. Seleznev ◽  
A.V. Tamrazova ◽  
◽  
...  
Keyword(s):  
Skin Barrier ◽  
Structural Features ◽  
Transepidermal Water Loss ◽  
General Information ◽  
Skin Barrier Function ◽  
Skin Physiology ◽  
Main Emphasis ◽  
Antimicrobial Protection ◽  
Irritant Dermatitis ◽  
Adaptive Shifts

The article provides general information about the skin physiology of newborns and infants. Structural features of the skin and main adaptive shifts in newborns, are described. Тhe child has an increase in the skin barrier function of the skin, which prevents transepidermal water loss; active synthesis of natural moisturizing factor (NMF) components that control skin hydration; shift of pH to acidic environment; normalization of thermoregulatory functions; enhancement of the photoprotective function; immune restructuring for antimicrobial protection; formation of a normal microbiome. The article describes the consequences of improper skin care of a newborn, using the example of diaper dermatitis, irritant dermatitis, prickly heat and vesiculopustulosis. The importance of using specialized children's cosmetics in caring for an infant is assessed. The basic recommendations for the choice of these products are presented, where the main emphasis is on the choice of products consisting of natural ingredients. Giving preference to natural cosmetics, everyone should carefully study the composition of these products and trust the manufacturers who can guarantee safety of care products for the youngest children.

Advances on chemically modified antimicrobial peptides for generating peptide antibiotics

2021 ◽  
Author(s):  
Samilla Rezende ◽  
karen k.n. oshiro ◽  
Nelson Junior ◽  
Octavio L. Franco ◽  
Marlon Henrique Cardoso
Keyword(s):  
Clinical Trials ◽  
Antimicrobial Peptides ◽  
Bacterial Infections ◽  
Peptide Antibiotics ◽  
Antibiotic Resistant ◽  
Chemically Modified

Antimicrobial peptides (AMPs) are pinpointed as promising molecules against antibiotic-resistant bacterial infections. Nevertheless, there is a discrepancy between the AMP sequences generated and the tangible outcomes in clinical trials. AMPs’...

ISSUES OF ETHNOCULTURAL TRADITIONS DEVELOPMENT IN MODERN ARTISTIC PRACTICE

2020 ◽  
Vol 1 (4) ◽  
pp. 50-54
Author(s):  
K. K. Murataev ◽  
◽  
S. M. Krykbayeva ◽  
Keyword(s):  
Folk Art ◽  
Structural Features ◽  
Artistic Practice ◽  
Creative Development ◽  
Traditional Art ◽  
Spatial Features ◽  
Applied Art ◽  
Main Components ◽  
Structurally Stable ◽  
Figurative Interpretation

The article deals with insufficiently studied theoretical aspects of revealing and analyzing new elements and motives of artistic and compositional means of expression in folk art, as well as topical issues concerning determining principles and possible options for their creative development in modern decorative and applied art, design, and artistic practice in general. The most important structural features of shaping in traditional art in the ontologically interrelated system "nature — man — object" are analyzed. The main structurally stable elements and components in Kazakh folk ornament are distinguished and characterized, these are the circle, S-shaped element, cruciform and triangular components. The evolution of their ideological and figurative interpretation and symbolism is traced, and their role in the genesis of folk art traditions formed over thousands of years is revealed. The selected basic elements of folk ornament, as the main components of means of artistic expression, are proposed to be defined and developed in line with modern interpretations of artistic and aesthetic categories — dynamics, statics, harmony and their decorative variations in accordance with the volumetric and spatial features of form.

scholarly journals In Vitro Activities of Designed Antimicrobial Peptides against Multidrug-Resistant Cystic Fibrosis Pathogens

1999 ◽  
Vol 43 (6) ◽  
pp. 1435-1440 ◽  
Author(s):  
Ute Schwab ◽  
Peter Gilligan ◽  
Jesse Jaynes ◽  
David Henke
Keyword(s):  
Cystic Fibrosis ◽  
Antimicrobial Peptides ◽  
Burkholderia Cepacia ◽  
Wide Spectrum ◽  
Multidrug Resistant ◽  
Medium Composition ◽  
Peptide Antibiotics ◽  
Rpmi Medium ◽  
Multidrug Resistant Pathogens

ABSTRACT The emergence of multidrug-resistant pathogens renders antibiotics ineffective in the treatment of lung infections in patients with cystic fibrosis (CF). Designed antimicrobial peptides (DAPs) are laboratory-synthesized peptide antibiotics that demonstrate a wide spectrum of antibacterial activity. Optimal conditions for susceptibility testing of these peptides have not yet been established. Medium composition is clearly a major factor influencing the results and reproducibilities of susceptibility tests. Using time-kill assays, we tested the effects of different media and buffers on the bactericidal activities of the peptides D2A21 and D4E1 onStaphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa ATCC 27853. Each peptide at 1 and 5 μM was incubated with bacteria in the different media and buffers. Both peptides were most active in Tris-HCl buffer against S. aureus andP. aeruginosa. Among the more complex media tested, modified RPMI medium was the medium in which the peptides demonstrated the highest activity, while it supported the growth of the bacteria. The broth microdilution technique was used to test the activities of D2A21 and D4E1 in modified RPMI medium against multidrug-resistant pathogens from patients with CF. The MICs of DAPs for methicillin-resistant S. aureus ranged from 0.25 to 4 μg/ml, those for multidrug-resistant P. aeruginosa ranged from 0.125 to 4 μg/ml, those for Stenotrophomonas maltophilia ranged from 0.5 to 32 μg/ml, and those forBurkholderia cepacia ranged from 32 to ≥64 μg/ml. When the activity of peptide D2A21 was compared with that of the tracheal antimicrobial peptide (TAP), D2A21 had greater potency than TAP againstP. aeruginosa. In addition, no difference in the MICs of D2A21 was seen when it was tested in nutrient broth supplemented with NaCl at different concentrations. Thus, DAPs are a class of salt-insensitive antibiotics potentially useful in the treatment of CF patients harboring multidrug-resistant P. aeruginosa.

scholarly journals Floristerias processing of mineral raw materials and waste Yaroslavsky mining and beneficiation plant

2017 ◽  
Author(s):  
Софья Ярусова ◽  
Sof'ya Yarusova ◽  
Павел Гордиенко ◽  
Pavel Gordienko ◽  
Иван Жевтун ◽  
...  
Keyword(s):  
Graduate Students ◽  
Alkali Metals ◽  
Raw Materials ◽  
General Information ◽  
Mineral Raw Materials ◽  
Beneficiation Plant ◽  
Fluoroammonium Salts ◽  
Beryllium Compounds ◽  
Main Components ◽  
Ore Dressing

The monograph presents general information on the main components of fluorite-containing ores and wastes and the results of studies on the possibility of complex processing of fluorite-containing mineral raw materials and wastes of the Yaroslavskiy ore-dressing combine with the extraction of beryllium compounds, alkali metals, fluoroammonium salts, as well as associated products in the form of gypsum, amorphous silica. Also, earlier studies related to this problem are summarized. The monograph is intended for specialists in the field of chemistry, chemical technology, ecology, scientists, graduate students, undergraduates involved in the complex processing of mineral raw materials and man-made wastes.

scholarly journals Revenues of Local Budgets in Ukraine: The Essential Component and the Current Situation in the Framework of the Budget Decentralization Reform

2020 ◽  
Vol 11 (514) ◽  
pp. 331-337
Author(s):  
V. A. Yankovskyi ◽  
◽  
M. T. Shendryhorenko ◽  
Keyword(s):  
Research Process ◽  
Current Situation ◽  
The European Union ◽  
Essential Component ◽  
Theoretical Generalization ◽  
State And Local ◽  
Local Budget ◽  
Decentralization Reform ◽  
Main Components ◽  
Budget Decentralization

The article is aimed at studying the essential component of decentralization and budget revenues; determining the main results of the implemented reform of budget decentralization in Ukraine; analyzing the current situation of own revenues of local budgets. The following general scientific methods were used in the research process: theoretical generalization, comparison and grouping – to determine the essence of the concepts of «budget revenues», «decentralization» and disclosure of components of budget revenues; analysis – when considering own revenues of local budgets for 2014–2020 and the structure of incomes in the revenues of local budgets for January – July 2020; system approach – in the formation and disclosure of the essences of the main stages of the reform of budget decentralization in Ukraine. The views of various scientists on the concepts of «budget revenues» and «decentralization» are examined. It is determined that budget revenues are an aggregate of financial relations that participate in the process of distribution of part of GDP in order to form targeted cash funds at the level of the State and local self-government bodies, designed to finance their own functions and obligations defined in accordance with the current legislation. The main components of budget revenues are determined and their classification is provided. The main stages of the budget decentralization reform in Ukraine are defined and systematized, their detailed characterizations are provided. The analysis of own revenues of local budgets for 2014–2020 and revenues in local budget revenues for January – July 2020 was carried out, during which it was determined that local taxes, despite the introduced budget decentralization, comprise only about 30% in the structure of incomes in the revenues of the general fund of local budgets, while in some countries of the European Union this figure reaches 50–60%. Practical steps towards further implementation of the budget decentralization reform for local self-government bodies are proposed.

scholarly journals Modulation of Neutrophil Apoptosis by Antimicrobial Peptides

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Isao Nagaoka ◽  
Kaori Suzuki ◽  
François Niyonsaba ◽  
Hiroshi Tamura ◽  
Michimasa Hirata
Keyword(s):  
Antimicrobial Peptides ◽  
Host Defense ◽  
Cell Activation ◽  
Immune Cell ◽  
Formyl Peptide Receptor ◽  
Bacterial Invasion ◽  
Neutrophil Apoptosis ◽  
Peptide Antibiotics ◽  
Nucleotide Receptor ◽  
Chemokine Production

Peptide antibiotics possess the potent antimicrobial activities against invading microorganisms and contribute to the innate host defense. Human antimicrobial peptides, α-defensins (human neutrophil peptides, HNPs), human β-defensins (hBDs), and cathelicidin (LL-37) not only exhibit potent bactericidal activities against Gram-negative and Gram-positive bacteria, but also function as immunomodulatory molecules by inducing cytokine and chemokine production, and inflammatory and immune cell activation. Neutrophil is a critical effector cell in host defense against microbial infection, and its lifespan is regulated by various pathogen- and host-derived substances. Here, we provided the evidence that HNP-1, hBD-3, and LL-37 cannot only destroy bacteria but also potently modulate (suppress) neutrophil apoptosis, accompanied with the phosphorylation of ERK-1/-2, the downregulation of tBid (an proapoptotic protein) and upregulation of Bcl-xL (an antiapoptotic protein), and the inhibition of mitochondrial membrane potential change and caspase 3 activity, possibly via the actions on the distinct receptors, the P2Y6 nucleotide receptor, the chemokine receptor CCR6, and the low-affinity formyl-peptide receptor FPRL1/the nucleotide receptor P2X7, respectively. Suppression of neutrophil apoptosis results in the prolongation of their lifespan and may be advantageous for the host defense against bacterial invasion.

scholarly journals Peptide Antibiotics Developed by Mimicking Natural Antimicrobial Peptides

2017 ◽  
Vol 06 (04) ◽  
Author(s):  
Nihan Unubol ◽  
Suleyman Selim Cinaroglu ◽  
Merve Acikel Elmas ◽  
Sumeyye Akcelik ◽  
Arzu Tugba Ozal Ildeniz ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Peptide Antibiotics ◽  
Natural Antimicrobial

scholarly journals Designed Antimicrobial Peptides Against Trauma-Related Cutaneous Invasive Fungal Wound Infections

2020 ◽  
Vol 6 (3) ◽  
pp. 184
Author(s):  
Kathryn W. Woodburn ◽  
Jesse M. Jaynes ◽  
L. Edward Clemens
Keyword(s):  
Antimicrobial Peptides ◽  
Broad Spectrum ◽  
Clinical Care ◽  
Structural Analog ◽  
Wound Infections ◽  
First Line ◽  
Empiric Treatment ◽  
Patients At Risk ◽  
Life Threatening ◽  
Traumatic Wound

Cutaneous invasive fungal wound infections after life-threatening dismounted complex blast injury (DCBI) and natural disasters complicate clinical care. These wounds often require aggressive repeated surgical debridement, can result in amputations and hemipelvectomies and have a 38% mortality rate. Given the substantial morbidity associated with cutaneous fungal wound infections, patients at risk need immediate empiric treatment mandating the use of rapidly acting broad-spectrum antimicrobials, acting on both fungi and bacteria, that are also effective against biofilm and can be administered topically. Designed antimicrobial peptides (dAMPs) are engineered analogues of innate antimicrobial peptides which provide the first line of defense against invading pathogens. The antifungal and antibacterial effect and mammalian cytotoxicity of seven innovative dAMPs, created by iterative structural analog revisions and physicochemical and functional testing were investigated. The dAMPs possess broad-spectrum antifungal activity, in addition to being effective against Gram-negative and Gram-positive bacteria, which is crucial as many wounds are polymicrobial and require immediate empiric treatment. Three of the most potent dAMPs—RP504, RP556 and RP557—possess limited mammalian cytotoxicity following 8 h incubation. If these encouraging broad-spectrum antimicrobial and rapid acting results are translated clinically, these novel dAMPs may become a first line empiric topical treatment for traumatic wound injuries.

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