scholarly journals Vegetative propagation of Nidularium fulgens Lem. in vitro.

1984 ◽  
Vol 32 (2) ◽  
pp. 101-106
Author(s):  
R.L.M. Pierik ◽  
H.H.M. Steegmans
Keyword(s):  
Vegetative Propagation ◽  
Shoot Tips ◽  
Media Composition ◽  
Liquid Media ◽  
Axillary Shoots ◽  
Solid Agar ◽  
Half Strength ◽  
Agar Media

Shoot tips were isolated and placed on solid agar media (half strength Murashige and Skoog macroelements, 3% sucrose, vitamins, 1 mg l-1 BA and 0.1 mg l-1 NAA). After one month, shoots which had begun to grow were transferred to liquid media (composition as above but with no agar and BA reduced to 0.3 mg l-1). After 12 weeks about 5 axillary shoots per explant were produced. Subsequent planting of these shoots in soil achieved almost 100% rooting. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Micropropagation of Isoplexis chalcantha Svent, O´Shanahan from Mature Plants

1970 ◽  
Vol 18 (2) ◽  
pp. 131-137 ◽  
Author(s):  
S. Mederos-Molina
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Nodal Segments ◽  
Axillary Shoots ◽  
In Vitro Shoots ◽  
Half Strength ◽  
Modified Ms Medium ◽  
High Degree ◽  
First Time

Culture medium requirements for micropropagation of Isoplexis chalcantha was achieved for the first time after high degree of contamination and phenolic exudates were detected and solved. Cultures were established from axillary shoots using juvenile branches collected from this medicinal plant. Most satisfying results were obtained using a solidified and a modified MS medium (NO3- : NH4+ ratios) enriched with ascorbic acid or soluble PVP plus GA3, BAP and NAA. Explants (nodal segments) were used for in vitro shoots multiplication and best results were achieved with modified MS plus BAP and auxins. Vigorous shoots rooted without symptoms in the half-strength modified MS enriched with low concentration of IBA. Key words: Isoplexis chalcantha, axillary shoots, contamination, phenolic exudates, culture media, NO3- : NH4+ ratios D.O.I. 10.3329/ptcb.v18i2.3395 Plant Tissue Cult. & Biotech. 18(2): 131-137, 2008 (December)

scholarly journals The influence of the mineral composition of the medium on in vitro propagation of Kohleria amabilis (Planch. et Linden) Fritsch shoots

2012 ◽  
Vol 60 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Danuta Kozak ◽  
Jerzy Hetman ◽  
Małgorzata Witek
Keyword(s):  
Mineral Composition ◽  
Significant Influence ◽  
In Vitro Propagation ◽  
Passage Time ◽  
Salt Composition ◽  
Mineral Salts ◽  
Axillary Shoots ◽  
Half Strength ◽  
Medium Type

The aim of the study was to test the influence of the mineral salt composition of the medium on <i>in vitro</i> multiplication and growth of kohleria shoots. Shoot tips were cultured on Murashige and Skoog (MS) (full- and half strength), Gamborg et al. (B<sub>5</sub>), Nitsch and Nitsch (NN) or Lloyd and McCown (WPM) media supplemented with BA 1 mg·dm<sup>-3</sup>. The influence of passage time on the induction and growth of shoots on the medium containing half strength of MS mineral salts and BA 1 mg·dm<sup>-3</sup>, GA<sub>3</sub> 1 mg·dm<sup>-3</sup> was also studied. The explants used in the experiments were obtained from aseptically grown shoot clusters. A significant influence of the medium type and the time of propagation on the number, length and weight of axillary shoots was observed. The medium containing full or half strength of mineral salts according to MS was the best. The propagation of kohleria shoots should take place at 4-week passages.

TWO MACHINES FOR IN VITRO PROPAGATION OF PLANTS IN LIQUID MEDIA

1983 ◽  
Vol 63 (1) ◽  
pp. 311-316 ◽  
Author(s):  
ROBERT E. HARRIS ◽  
EDWIN B. B. MASON
Keyword(s):  
Nicotiana Tabacum ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Method ◽  
The Other ◽  
Liquid Media ◽  
Wide Mouth ◽  
Agar Media ◽  
Two Machines

Two machines are described, one to tilt 400 50-mL or 320 125-mL Erlenmeyer flasks, and the other to rock 120 455-mL or 70 910-mL square wide-mouth Mason jars, or combinations of the different container sizes. In grapes, explants transferred to liquid media on the machines after 28 days on agar produced seven times as many shoots in 90 days as explants maintained on agar media. Similar increases were obtained with Arctostaphylos uva ursi, Fuchsia hybrida ’Swingtime,’ Amelanchier alnifolia, and Nicotiana tabacum ’Xanthi-nc.’ The liquid culture method also reduces cost by using less media and agar.Key words: Machines, in vitro, propagation, liquid-media

scholarly journals Micropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species

1970 ◽  
Vol 16 (1) ◽  
pp. 19-23 ◽  
Author(s):  
S Mederos-Molina
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plant ◽  
Plant Species ◽  
Culture Medium ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Axillary Shoots ◽  
Half Strength ◽  
Important Medicinal Plant

The purpose of this study was to establish culture medium requirements for micropropagation of Salvia broussonetii Benth., an important medicinal plant. Cultures were initiated from axillary shoots collected from mature plants. Most satisfactory results were achieved using a MS.2 medium supplemented with 1 mM ascorbic acid, 1.44 µM GA3 and 1.11 µM BAP. Axillary nodes were used for in vitro regeneration of multiple shoots and best results were achieved with MS.2 medium plus 1.44 µM GA3, 2.66 µM BAP and 1.14 µM IAA. Shoots rooted without symptoms of chlorosis or necrosis in half-strength MS.2 medium plus 1.44 µM GA3 and 2.28 µM IAA.Key words: Axillary shoots, Micropropagation, Medicinal plant, Salvia broussonetiiDOI = 10.3329/ptcb.v16i1.1101Plant Tissue Cult. & Biotech. 16(1): 19-23, 2006 (June)

scholarly journals An Efficient In Vitro Propagation Protocol of Cocoyam [Xanthosoma sagittifolium(L) Schott]

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Anne E. Sama ◽  
Harrison G. Hughes ◽  
Mohamed S. Abbas ◽  
Mohamed A. Shahba
Keyword(s):  
Dimethyl Sulfoxide ◽  
In Vitro Propagation ◽  
Apical Meristem ◽  
Culture Medium ◽  
Naphthaleneacetic Acid ◽  
Liquid Media ◽  
Mineral Salts ◽  
Axillary Shoots ◽  
Xanthosoma Sagittifolium

Sprouted corm sections of “South Dade” white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3–5 mm comprising the apical meristem with 4–6 leaf primordial, and approximately 0.5 mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg’s B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA) were used throughout the study. Thidiazuron (TDZ) solution containing 0.01% dimethyl sulfoxide (DMSO) was used. Erlenmeyer flasks and test tubes were used for growing cultures. The effect of different media substrate, thidiazuron, and the interaction between TDZ and Benzylaminopurine (BAP) on cocoyam culture were tested. Results indicated that cocoyam can be successfully micropropagated in vitro through various procedures. All concentrations tested (5–20 μM BAP and 1–4 μM TDZ) produced more axillary shoots per shoot tip than the control without cytokinins. Greater proliferation rates were obtained through the use of 20 μM BAP and 2 μM TDZ, respectively, 12 weeks from initiation. Shoots produced with BAP were larger and more normal in appearance than those produced with TDZ, which were small, compressed, and stunted. The use of stationary liquid media is recommended for economic reasons.

scholarly journals Regeneration of Ficus glomerata Roxb., using shoot tips and nodal explants

1970 ◽  
Vol 39 (1) ◽  
pp. 47-50 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Ficus Glomerata

Shoot tips and nodal explants from in vitro growing seedlings of Ficus glomerata Roxb. (Moraceae). showed best shoot induction (88%) on MS medium supplemented with 0.5 mg/l BAP, where maximum number of shoots were produced per culture. In vitro raised shoots rooted well on half strength of MS medium with 2.0 mg/l IBA + 0.1 mg/l NAA. The survival rate of regenerated plantlets was 82%. Key words: Ficus glomerata Roxb.; Shoot proliferation; Micropropagation; Acclimatization DOI: 10.3329/bjb.v39i1.5525Bangladesh J. Bot. 39(1): 47-50, 2010 (June)

Micropropagation of some Myrtaceae species which show potential as 'new' ornamental plants

1993 ◽  
Vol 33 (3) ◽  
pp. 385 ◽  
Author(s):  
SS Speer
Keyword(s):  
Ornamental Plants ◽  
Shoot Proliferation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Soil Culture ◽  
Axillary Shoots ◽  
Half Strength ◽  
Roots In Vitro ◽  
Chamelaucium Uncinatum

Eleven species of Australian Myrtaceae were evaluated for their ability to be cultured in vitro. Ten species produced axillary shoots (microcuttings) suitable for inducing roots in vitro. Microcuttings of 9 species successfully developed roots and were transferred to soil culture in a glasshouse, where plants grew normally. Nodal explants were grown on a modified Murashige and Skoog (MS) medium supplemented with varying concentrations of 6-benzylaminopurine (BAP), to study shoot proliferation. Beaufortia heterophylla Turcz. explants did not respond to BAP, and all explants eventually died. The rate of shoot proliferation for the other species varied according to BAP concentration. Microcuttings of 10 species were grown on a modified half-strength MS medium supplemented with varying concentrations of the auxins indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA), to induce root formation. An increase in root number and an associated decrease in root length was observed as the concentration of IBA and NAA was increased. Verticordia muelleriana E. Pritzel did not develop roots in any treatment. Chamelaucium uncinatum Schauer cv. Purple Pride, Kunzea parvifolia Schauer, K. pulchella (Lindl.) A. S. George, Leptospermum rotundifolium (Maiden & Betche) F. Rodway ex Cheel, Verticordia drunzmondii Schauer, V. graizdis J. L. Drumm., V. hughanii F. Muell., V. nzonaclelpha Turcz., and V. roeii Endl. microcuttings developed roots both with and without added auxins. Roots that formed on microcuttings at higher auxin concentrations were generally thicker and slower in growth.

scholarly journals Induksi Poliploidi Phalaenopsis amabilis (L.) Blume dan Phalaenopsis amboinensis J. J. Smith dengan Kolkisin dalam Kultur In Vitro

2016 ◽  
Vol 43 (3) ◽  
pp. 219 ◽  
Author(s):  
Eka Martha Della Rahayu ◽  
Dewi Sukma ◽  
Muhamad Syukur ◽  
Rawati ,
Keyword(s):  
Stomatal Density ◽  
Block Design ◽  
Liquid Media ◽  
Stomatal Size ◽  
Stomata Size ◽  
Randomized Complete Block Design ◽  
Murashige Skoog ◽  
Half Strength ◽  
Number Of Leaves

<p>ABSTRACT</p><p>Phalaenopsis amabilis (L.) Blume and Phalaenopsis amboinensis J.J. Smith (diploid) are important in Phalaenopsis breeding. Polyploid species are needed for crossing with polyploid hybrid varities of Phalaenopsis. The objectives of this study were to obtain effective concentration of colchicine to induce polyploidy and to produce polyploid plantlets of P. amabilis and P. amboinensis. Experiment was arranged in randomized complete block design with one factor, the colchicine concentration. Protocorms of P. amabilis and P. amboinensis were immersed in half strength of Murashige-Skoog (1/2 MS) liquid media added with colchicine (0; 0.5; 5; 25; 50, and 75 mg L-1) for 10 days. The results showed that higher concentration of colchicine on both species did not have significant effect on the survival of the plantlets at 24 weeks after treatment. The average number of leaves and roots of colchicine treated planlets from both species were less than the control plantlets. Immersing protocorm in colchicine at concentration of 50 mg L-1 for 10 days was effective in inducing polyploid plantlets of P. amabilis and P. amboinensis with the frequency of 33.3% and 40%, respectively. Polyploid plantlet has larger stomata size and lower stomata density than the diploid ones.</p><p>Keywords: chromosome number, colchicine, polyploid, protocorm, stomatal density, stomatal size</p>

scholarly journals Micropropagation of Antelope Bitterbrush [Purshia tridentata (Pursh) DC.]

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 312-314 ◽  
Author(s):  
John L. Edson ◽  
David L. Wenny ◽  
Annette Leege-Brusven
Keyword(s):  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Axillary Shoots ◽  
Full Strength ◽  
Woody Plant Medium ◽  
Half Strength ◽  
In Vitro Survival

In vitro—derived microshoots of antelope bitterbrush, incubated for 1 month in media supplemented with 0.44 μm BA, grew 0.8 and 1.1 cm longer in woody plant medium (WPM) compared to full-strength and half-strength Murashige and Skoog (MS) media, respectively. Explants cultured in WPM supplemented with 0.44 μm BA and 0.54 μm NAA produced a mean of five axillary shoots per explant. Explants dipped in 0.1% IBA or 0.1% NAA rooted best in 0.1% IBA with 89% success ex vitro vs. 60% success in vitro. Survival of acclimatized plantlets rooted ex vitro was 95%, while 50% survived when rooted in vitro. After 1 year of greenhouse growth, 98% of plantlets survived and flowered. Chemical names used: benzyladenine (BA), 3-indolebutyric acid (IBA), 1-naphthaleneacetic acid (NAA).

scholarly journals Micropropagation of Raspberries (Rubus idaeus L.) in Liquid Media by Temporary Immersion Bioreactor in Comparison with Gelled Media

2021 ◽  
Vol 78 (2) ◽  
pp. 56
Author(s):  
Doina CLAPA ◽  
Monica HÂRȚA ◽  
Cornel Viorel POP
Keyword(s):  
Large Scale ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Liquid Media ◽  
Temporary Immersion Bioreactor ◽  
In Vitro Proliferation ◽  
Axillary Shoots ◽  
Temporary Immersion ◽  
Experimental Treatments

Temporary Immersion Bioreactor (TIB) is a suitable technique for large scale micropropagation of plant species. The aim of this work was to test the capacity of in vitro proliferation of the primocane-fruiting red raspberry cv Maravilla and floricane-fruiting red raspberry cv Willamette on gelled media compared to liquid media. The two varieties were cultured in vitro on two media, Murashige and Skoog 1962 (MS) and Driver and Kuniyuki walnut medium, 1984 (DKW), both supplemented with 0.5 mg/l 6-benzyladenine (BA). In the control cultures on gelled media the media were gelled with 5g/l Plant Agar, whereas for the cultures in liquid media Plantform bioreactors were used. After six weeks of in vitro culture we recorded the proliferation rates and lengths of the axillary shoots obtained in all the experimental treatments. The highest proliferation rate was 16 ± 2.03, obtained in cv. Willamette on gelled MS medium with 0.5 mg/l BA. The longest shoots (3.17 ± 0.32 cm) were obtained at cv. Maravilla on the DKW medium with 0.5 mg / l BA in the bioreactor. Our research highlighted that Rubus idaeus L. Maravilla and Willamette can be TIB propagated, although further research is needed to improve the efficiency of this method.

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