scholarly journals Induksi Poliploidi Phalaenopsis amabilis (L.) Blume dan Phalaenopsis amboinensis J. J. Smith dengan Kolkisin dalam Kultur In Vitro

2016 ◽  
Vol 43 (3) ◽  
pp. 219 ◽  
Author(s):  
Eka Martha Della Rahayu ◽  
Dewi Sukma ◽  
Muhamad Syukur ◽  
Rawati ,
Keyword(s):  
Stomatal Density ◽  
Block Design ◽  
Liquid Media ◽  
Stomatal Size ◽  
Stomata Size ◽  
Randomized Complete Block Design ◽  
Murashige Skoog ◽  
Half Strength ◽  
Number Of Leaves

<p>ABSTRACT</p><p>Phalaenopsis amabilis (L.) Blume and Phalaenopsis amboinensis J.J. Smith (diploid) are important in Phalaenopsis breeding. Polyploid species are needed for crossing with polyploid hybrid varities of Phalaenopsis. The objectives of this study were to obtain effective concentration of colchicine to induce polyploidy and to produce polyploid plantlets of P. amabilis and P. amboinensis. Experiment was arranged in randomized complete block design with one factor, the colchicine concentration. Protocorms of P. amabilis and P. amboinensis were immersed in half strength of Murashige-Skoog (1/2 MS) liquid media added with colchicine (0; 0.5; 5; 25; 50, and 75 mg L-1) for 10 days. The results showed that higher concentration of colchicine on both species did not have significant effect on the survival of the plantlets at 24 weeks after treatment. The average number of leaves and roots of colchicine treated planlets from both species were less than the control plantlets. Immersing protocorm in colchicine at concentration of 50 mg L-1 for 10 days was effective in inducing polyploid plantlets of P. amabilis and P. amboinensis with the frequency of 33.3% and 40%, respectively. Polyploid plantlet has larger stomata size and lower stomata density than the diploid ones.</p><p>Keywords: chromosome number, colchicine, polyploid, protocorm, stomatal density, stomatal size</p>

scholarly journals Pertumbuhan Planlet Nenas (Ananas comosusL. Merr.) Varietas Smooth Cayenne Hasil Kultur In Vitro pada Beberapa Konsentrasi BAP dan Umur Plantlet

2013 ◽  
Vol 1 (1) ◽  
pp. 54
Author(s):  
Ramadhani Dwi Santoso ◽  
. Sobir
Keyword(s):  
Block Design ◽  
Error Level ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Difference Test ◽  
Honestly Significant Difference Test

<p style="text-align: justify;">The aim of this research is to study the effects of synthetic cytokinin (6-benzylaminopurine/BAP) treatments on two groups of pineapple (Ananas comosusL.Merr.) plantlets differentiated by plantlet ages. The research used the Factorial Experiment on Randomized Complete Block Design with two factors, which are BAP concentrations (0 ppm, 25 ppm, 50 ppm, and 75 ppm) and plantlet division by age grouping (52 days and 69 days), with three replications. It was then followed by Tukey’s Honestly Significant Difference Test at error level of 5%. The results show that BAP treatments with the concentration used in this research significantly inhibits the growth of pineapple plantlets observed on four variables (number of leaves, leave length, plantlet height, and plantlet diameter), while age grouping treatments didn’t show any significant effects, except on the plantlet height at 8 and 14 weeks after treatment. There are also treatment-related interactions which significantly affects the number of leaves and plantlet height. It was then suggested that lower concentrations of cytokinin are required if similar research is to be conducted in the future.</p><p>Keywords : pineapple, post-acclimatization, propagation, 6-benzylaminopurine</p>

332 Effect of Chemical and Biological Preservatives on in vitro Fermentation and Methane Production of Ensiled and Aerobically Exposed Wet Brewer’s Grains

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 182-182
Author(s):  
Marjorie A Killerby ◽  
Diego Zamudio ◽  
Kaycee Ames ◽  
Darren D Henry ◽  
Thomas Schwartz ◽  
...  
Keyword(s):  
Methane Production ◽  
Production System ◽  
Methane Concentration ◽  
Block Design ◽  
Gas Production ◽  
Sodium Lignosulfonate ◽  
In Vitro Fermentation ◽  
Randomized Complete Block Design ◽  
System Data

Abstract This study evaluated the effects of preservatives on the in vitro fermentation measures of wet brewer’s grain (WBG) silage at different stages of storage. Treatments (TRT) were sodium lignosulfonate at 1% (NaL1) and 2% (NaL2; w/w of fresh WBG), propionic acid (PRP; 0.5% w/w of fresh WBG), a combination inoculant (INO; Lactococcus lactis and Lactobacillus buchneri each at 4.9 log cfu/fresh WBG g), and untreated WBG (CON). WBG (Fresh) were packed into 8.8 L mini-silos and stored for 60 d at 21°C (Ensiled), then they were opened and aerobically exposed for 10d (AES). Samples from each stage of storage (STG; Fresh, Ensiled and AES) were analyzed for in vitro ruminal digestibility (24 h).Gas kinetics were recorded using the Ankom RF Gas Production System. Data were analyzed as a randomized complete block design (5 blocks) with a 5 (TRT) × 3 (STG) factorial arrangement. Apparent in vitro DM digestibility (DMD) decreased across STG, (51.5, 47.2 and 40.9 for Fresh, Ensiled and AES, respectively) and increased for NaL1, NaL2 and PRP (~47.8) vs. CON (43.0 ± 2.12%). PRP increased apparent in vitro OM digestibility (OMD) when Ensiled (54.5) and NaL2 increased it for AES (47.1) vs CON (46.3 and 39.9 ± 1.73%, respectively). The asymptotic maximal (M) and rate (k) of gas production decreased across STG (214.6, 181.5, 155.1 and 14.6, 12.6, and 9.8, for Fresh, Ensiled and AES, respectively). PRP increased (200.0) and NaL1 decreased (169.3) M vs. CON (183.9± 7.81ml/incubated DM g), while NaL1 and NaL2 (~11.4) decreased k vs. CON (13.4 ± 0.85%/h). Methane concentration and yield were higher in Fresh vs. other STG (0.94 vs. ~0.84 ± 0.07mM and 0.27 vs. ~0.23 ± 0.03mmol/g fermented OM). Spoilage of WBG decreases fermentability and methane production while PRP and NaL improve digestibility with the former also increasing M and k.

scholarly journals Effect of Zinc and Boron on Growth and Yield of Okra (Abelmoschus esculentus L.)

2020 ◽  
pp. 41-47
Author(s):  
Nusrat Jahan ◽  
Md. Ashabul Hoque ◽  
Md. Rasal-Monir ◽  
Sumya Fatima ◽  
Mohammad Nurul Islam ◽  
...  
Keyword(s):  
Dry Matter ◽  
Block Design ◽  
Fruit Weight ◽  
Fruit Yield ◽  
Growth And Yield ◽  
Area Index ◽  
Spad Value ◽  
Randomized Complete Block Design ◽  
Number Of Leaves ◽  
Fruit Diameter

The study was carried out to find out the effect of zinc (Zn) and boron (B) on growth and yield of okra (BARI Dherosh 1). The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. The treatments of the experiment were, T0 (without Zn or B), T1 (20 kg Zn ha-1), T2 (30 kg Zn ha-1), T3 (10 kg B ha-1), T4 (20 kg B ha-1), T5 (20 kg Zn ha-1 + 10 kg B ha-1), T6 (20 kg Zn ha-1 + 20 kg B ha-1), T7 (30 kg Zn ha-1 + 10 kg B ha-1) and T8 (30 kg Zn ha-1 + 20 kg B ha-1) were undertaken to evaluate the best results of the study. The highest plant height was found in T8 (30 kg Zn ha-1 + 20 kg B ha-1) but the highest number of leaves plant-1 was recorded from T7 (30 kg Zn ha-1 + 10 kg B ha-1). On the other hand, the maximum leaf area index, SPAD value, mean fruit weight, fruit length, fruit diameter, fruit dry matter (%), number of fruits plant-1, fresh fruit weight plant-1 , fruit yield plot-1 and fruit yield ha-1 were found in T7 (30 kg Zn ha-1 + 10 kg B ha-1), while the control (T0) showed lowest performance for the respected parameters. It is strongly concluded that 30 kg Zn ha-1 with 10 kg B ha-1 combination may be helpful for okra cultivation in the field level to increase okra production.

scholarly journals Effect of Seeding Rate On Growth And Yield Of Okra (Abelmoschus Esculantus) In Zuru Northern Guinea Savanna Of Nigeria

2018 ◽  
Vol 6 (02) ◽  
Author(s):  
Yohanna J, A. ◽  
Muhammad A, M.
Keyword(s):  
Block Design ◽  
Total Yield ◽  
Growth And Yield ◽  
Land Resource ◽  
Seeding Rate ◽  
Randomized Complete Block Design ◽  
Number Of Leaves ◽  
Resource Space ◽  
Plant Stand ◽  
The Mean

Field experiment was carried out during 2010 rainy season to find the effect of seeding rate on the growth and yield of okra (Abelmuschus esculentus) in Zuru Northern Guinea Savannah. Zuru is located in Zuru Local government area of Kebbi State (Latitude 11o 251 to 11o 551 North and Longitude 4o 51 to 5o 251 East of the equator). The experiment consisted of three treatments that is planting okra at one plant per stand (S1), two plants per stand (S2) and three plants per stand (S3). The experiment was laid out in randomized complete block design (RCBD) and the treatments replicated three time. The result of the experiment has shown that plant height and number of leaves/plant at 3, 6 and 9 weeks after planting (    WAP) were significantly influenced by seeding rate (P<0.05). At 3, 6 and 9 WAP tallest plants were obtained from okra plants planted at three plants/stand (27.83, 54.0 and 77.0cm) respectively followed by okra planted at two plant/stand (24.77, 50.67 and 74.33 leave/plant respectively ). However, more number of leaves/plant were obtained from okra plant at one plant/stand (9.67, 24.67 and 48.0 leaves/plant respectively) followed by okra planted at two plants/stand (8.33, 18.33 and 36.33 leaves/plant respectively). Seeding rate had significant influence on time of 50 percent flowering with one plant/stand first attaining 50 percent flowering stage just within 36.33 days followed by okra planted at two plants/stand (38 days). The mean fresh fruit yield of okra was also significantly influenced by seeding rate with the highest yield of fresh fruits obtained from three plants/stand (83.34/ha) followed by okra planted at two plants/stand (55.56/ha). However, the result of the findings recommends that, okra should be planted at two plants/stand for medium sized and marketable okra fresh fruits since at three plants/stand leads to the production of undersized fresh fruits which do not often attract customers. At one plant/stand, there seemed to be early fruiting and bigger sized fresh fruits but low total yield due to underutilization of land resource/space. Planting at one plant/stand should therefore be avoided

scholarly journals Growth Performance of Lettuce Using Fermented Products as Organic Fertilizers

2019 ◽  
Vol 1 (3) ◽  
pp. 65
Author(s):  
Kareen Lynn E. Negado
Keyword(s):  
Growth Performance ◽  
Block Design ◽  
Organic Fertilizers ◽  
Control Groups ◽  
Lettuce Seeds ◽  
Randomized Complete Block Design ◽  
Number Of Leaves ◽  
The Mean ◽  
And Control ◽  
Fermented Products

This study evaluates the growth performance of lettuce (Lactuca sativa L.) utilizing various fermented products as organic fertilizers. Growth performance is determined through the plant’s weight and leaves and significant differences in growth among various treatments: (a) fish trash, (b) scrap seaweeds, (c) kangkong, and control groups urea (positive) and water (negative) was also determined. Kangkong leaves are fermented for 10 days, fish trash for 14 days and 1 month for seaweeds. Lettuce seeds are sown before transplanting (15 days after germination) into pots and placed in raised beds. Randomized complete block design is utilized in the experiment. Growth performance is measured through the number of leaves 10, 20, and 30 days after transplant and mass of plant upon harvest. After 30 days, the lettuce was harvested. Based on the findings, the soil and fermented products are acidic and the needed organic matter for optimum growth of lettuce are not sufficient as well as some nutrients of the fermented products. Among the organic fertilizers, kangkong at 30 ml performs best in terms of the growth performance of lettuce. Urea performs best in terms of the mass upon harvest. Significant differences exist in the mean number of leaves of lettuce treated with various amounts of organic fertilizers with the plants that receive urea. Lettuce produce more number of leaves and with the highest mass upon harvest when treated with urea compared with organic fertilizers applied after 30 days from transplant. Keywords: fermented kangkong; fish trash; growth performance; lettuce; scrap seaweeds

scholarly journals Evaluation of Sweet Pepper (Capsicum annum L.) Varieties as Influnced by Nitrogen Levels in Chitwan, Nepal

2020 ◽  
Vol 14 (1) ◽  
pp. 33-42
Author(s):  
Santosh Lohani ◽  
Moha Dutta Sharma ◽  
Shree Chandra Shah ◽  
Arjun Kumar Shrestha
Keyword(s):  
Block Design ◽  
Total Yield ◽  
Growth Yield ◽  
Sweet Pepper ◽  
Fruit Weight ◽  
Chemical Fertilizer ◽  
Nitrogen Levels ◽  
Randomized Complete Block Design ◽  
Number Of Leaves ◽  
Better Than

An experiment was conducted to assess the performance of sweet pepper varieties as influenced by nitrogen levels from August 2014 to February 2015 at Mangalpur, Chitwan. The experiment was laid out in two factorial Randomized Complete Block Design (RCBD) with three replications having sixteen treatments. Varieties and levels of nitrogen significantly affected growth, yield and qualitative parameters of fruits. Hybrid Indra and NS 632 performed better than open pollinated varieties viz. Sagar and California Wonder. Days to fifty percent flowering of NS 632 were earlier than California Wonder. Indra variety had the highest plant height (52.18 cm), number of secondary branches per plant (13.51) from 250 kg N/ha through chemical fertilizer while number of leaves per plant was higher in NS 632 variety (57.22) than Sagar variety (47.37). Indra variety showed the highest fruit weight (87.59 g) and total yield (39.45 t/ha) but NS 632 variety produced more number of fruits per plant (9.25) than California Wonder variety. This study showed that sweet pepper yield of hybrid varieties applied with 250 kg N/ha through chemical fertilizer was appropriate dose to get optimum yield under Chitwan condition.

scholarly journals Peculiarities of in vitro rooting of some species of Carlina L. genus

1970 ◽  
pp. 215-220
Author(s):  
N. B. Kravets ◽  
M. Z. Mosula ◽  
N. M. Drobyk ◽  
N. V. Tulaidan ◽  
M. B. Cheterbok
Keyword(s):  
Acid Solution ◽  
Growth Regulators ◽  
Root Formation ◽  
Cultivated Plants ◽  
Treat Ment ◽  
Presowing Treatment ◽  
Murashige Skoog ◽  
Half Strength ◽  
Semi Solid

Aim. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treat-ment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentra-tions of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators. Keywords: Carlina àcaulis L., Carlina cirsioides Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, sprouts rooting.

scholarly journals PRODUCTION AND NUTRITIVE VALUE OF CANARANA ERECTA LISA (Echinocloa piramidalis Lam.) IN RESPONSE TO HARVEST INTERVALS

2019 ◽  
Vol 20 ◽  
Author(s):  
Angela Maria de Vasconcelos ◽  
Mauricio Cesio Araujo Dutra ◽  
Robson Mateus Freitas Silveira ◽  
Valdson José da Silva ◽  
Luís Alfredo Pinheiro Leal Nunes ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Experimental Unit ◽  
Forage Production ◽  
Dry Matter Digestibility ◽  
Forage Digestibility ◽  
Randomized Complete Block Design ◽  
Harvest Intervals

Abstract The objective of this study was to compare and explain the variations in the production and nutritive value of canarana erecta lisa grass (Echinocloa pyramidalis Lam.) at different harvest intervals. The treatments corresponded to the cut intervals of 20, 27, 34, 41, 48, 55, 62, and 69 days. The experimental design was a randomized complete block design with three replications. Each experimental unit had 6m2 (3m ×2m). The variables studied included forage production, canopy height, and nutritive value o harvested forage. Forage production varied from 11517 to 12324 kg ha-1; Plant heights from 48.8 to 234 cm; Neutral detergent fiber (NDF) from 68.8% to 71.13%; Acid detergent fiber (ADF) from 36.20% to 44.15%; Crude protein (CP) from 11.43% to 5.85% and in “in vitro” dry matter digestibility (IVDMD) from 63.30% to 47.42%. These values indicated that the nutritive value of canarana erecta lisa was reduced at longer harvest intervals. It is recommended to harvest the grass at 35 days of regrowth favoring productivity, nutritive value and forage digestibility.

scholarly journals Growth of escarole in different seasons of year, RS, Brazil

2018 ◽  
Vol 39 (2) ◽  
pp. 455
Author(s):  
Daniele Cristina Fontana ◽  
Braulio Otomar Caron ◽  
Denise Schmidt ◽  
Daniela Meira ◽  
Thais Pollon Zanatta ◽  
...  
Keyword(s):  
Block Design ◽  
Global Radiation ◽  
Technical Information ◽  
Dry Mass ◽  
Four Seasons ◽  
Randomized Complete Block Design ◽  
Different Seasons ◽  
Crop Cycle ◽  
Number Of Leaves ◽  
Santa Maria

The lack of research about escarole cultivars adapted to each region, as well as technical information on plant growth have been some of the factors related to low yields. Therefore, the aim of this work was to evaluate the growth of escarole crop in four seasons of year in Santa Maria, RS. The experiment was conducted in a randomized complete block design, 1 x 4, being a escarole (cultivar Escorola Lisa®) evaluated in four seasons of year (summer, autumn, winter and spring), in six replications. The evaluations were performed in a destructive manner every seven days, from date of transplant to harvest point. The following components were analyzed: number of leaves, number of residues, dry mass of leaves, dry mass of leaves, stem and residue. Thus, it was determined the parameters for growth analysis. With the data obtained, we performed a variance analysis, and presented significant results, and the means of each season of the year submitted to the Tukey test at 5% of error probability. The air temperature and global radiation influence crop cycle. Lower temperatures and lower incidence of radiation result in cycle extended, as observed in autumn. The escarole growth is influenced by seasons of year in Rio Grande do Sul (RS), and summer and spring finish the cycle in advance to others, presenting a larger leaf area. Summer and spring are most indicated season to escarole crop in RS, Brazil.

scholarly journals GENETIC DIVERSITY OF POTATO (Solanum tuberosum L.)

2016 ◽  
Vol 29 (1) ◽  
pp. 39-43
Author(s):  
M. H. Rahman ◽  
M. S. Islam ◽  
S. Sonom
Keyword(s):  
Solanum Tuberosum L ◽  
Block Design ◽  
Soluble Sugar ◽  
Maximum Performance ◽  
Total Soluble Sugar ◽  
Randomized Complete Block Design ◽  
Cluster Distance ◽  
Percentage Weight ◽  
Number Of Leaves ◽  
Hybridization Programme

The experiment was conducted with twenty one genotypes of potato at the experimental farm of Sher-e-Bangla Agricultural University, Dhaka during the period from November 2014 to March 2015 to estimate the genetic variability and diversity among the genotypes. The experiment was conducted using Randomized Complete Block Design with three replications. All the genotypes varied significantly with each other for all the studied characters indicated the presence of considerable variations among the genotypes studied As per PCA, D2 and clusters analysis the genotypes were grouped into five different clusters. Clusters III had the maximum nine and cluster II and V had the minimum one genotype. The highest inter-cluster distance was observed between I and V and the lowest was observed between IV and V. Genotypes in cluster I showed the maximum performance for number of leaves per plant, chlorophyll percentage, weight of individual potato and firmness. Cluster II showed maximum performance for total soluble sugar. Cluster III recorded the highest mean performance for dry matter. Cluster IV showed the maximum performance for number of potato per hill, weight of potato per hill and yield. Cluster V showed the maximum performance for specific gravity. Considering this idea and other characteristic performances, G8 (Shada pakri) and G17 (Shil bilati) from cluster IV; G4 (BARI-TPS-1) from cluster II; and G6 (Asterix), G21 (Granola) , G1 (Cardinal) and G2 (Diamant) from cluster I might be considered better parents for efficient hybridization programme.

Sign in / Sign up

Export Citation Format

Share Document