scholarly journals Role of oxidative stress in cataractogenesis

2017 ◽  
Vol 5 (6) ◽  
pp. 2390 ◽  
Author(s):  
Geeta Bhatia ◽  
A. N. Sontakke ◽  
Subodhini Abhang
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Protein Carbonyl ◽  
Diabetic Patients ◽  
Diabetic Cataract ◽  
Age Related ◽  
Antioxidant Defence System ◽  
Cataract Patients

Background: Cataract is a multifactorial disease and is a major cause of blindness in india. Oxidative stress is thought to be a major factor to initiate the process of cataractogenesis. It is today well established fact that oxidative stress participates in both age-related (senile) and diabetes-induced cataract (diabetic). Oxidative damage to the lens most likely arises as a consequence of an impaired antioxidant defence system, due to increased generation of ROS both by age and diabetes. The present study was designed to determine role of oxidative stress in cataractogensis and to compare levels of oxidative stress markers in senile and diabetic cataract patients.Methods: Serum malondialdehyde (MDA) and serum protein carbonyl (PC) were measured as indicator of oxidative stress whereas antioxidant status was assessed by estimating serum Total antioxidant capacity (TAC) and dietary antioxidants levels i.e vitamin C and vitamin E in senile and diabetic cataract patients compared with healthy controls.Results: The result reveal that the serum MDA and PC levels were significantly increased in patients with senile and diabetic cataract whereas serum TAC, vitamin C and Vitamin E were significantly reduced in senile and diabetic patients when compared with normal healthy controls.Conclusions: From the result, it is concluded that oxidative stress is in the foreground of cataract formation which includes senile and diabetic cataract. Oxidative stress produced in diabetic cataract patients is more as compared to senile cataract patients. 

scholarly journals Comparison of Oxidant-Antioxidant Status in Patients with Vitiligo and Healthy Population

2015 ◽  
Vol 12 (2) ◽  
pp. 132-136 ◽  
Author(s):  
S Agrawal ◽  
A Kumar ◽  
TK Dhali ◽  
SK Majhi
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Control Group ◽  
Healthy Population ◽  
Significant Difference ◽  
Destruction Of Melanocytes ◽  
And Control

Background Vitiligo is a well-recognized pigmentary disorder of the skin and /or mucous membrane characterized by circumscribed ivory or chalky white macules devoid of identifiable melanocytes. The pathogenesis of vitiligo is complex and still not well understood. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration. The role of free radicals and oxidative damage in the pathophysiology of vitiligo has been documented in recent studies.Objective To evaluate the role of oxidative stress in patients with vitiligo and of healthy controls by measuring levels of the oxidant malondialdehyde (MDA) and antioxidants vitamin C and vitamin E in serum and catalase (CAT) in erythrocytes.Method A total of 80 clinically diagnosed cases of vitiligo and 80 control subjects were included in the study to assess the activity of MDA, vitamin C and vitamin E in serum and CAT in erythrocytes of patients and controls by using the spectrophotometric assay.Result There was statistically significant increase in the levels of MDA in patients with vitiligo compared to the control group (p<0.001). No significant difference was found in the levels of vitamin C (p=0.411) and vitamin E (p=0.771) between the patients with vitiligo and control group. The levels of CAT in the vitiligo patients were found to be significantly lower than those of controls (p<0.001).Conclusion Increased oxidative stress and decreased catalase have been observed in vitiligo patients and the data suggesting that the free radicals may be involved in the destruction of melanocytes or dysregulation of melanogenesis.Kathmandu University Medical Journal Vol.12(2) 2014: 132-136

scholarly journals Serum Antioxidative Enzymes Levels and Oxidative Stress Products in Age-Related Cataract Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Dong Chang ◽  
Xuefei Zhang ◽  
Shengzhong Rong ◽  
Qian Sha ◽  
Peipei Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidative Enzymes ◽  
Degradation Products ◽  
Statistical Significance ◽  
Protein Carbonyl ◽  
Serum Samples ◽  
Control Subjects ◽  
Age Related ◽  
And Oxidative Stress ◽  
Cataract Patients

Purpose. To investigate the activity of antioxidative enzymes and the products of oxidative stress in patients with age-related cataracts and compare the findings with those in healthy control subjects.Method. Sixty patients with age-related cataract and sixty healthy controls of matched age and gender were included in this study. Serum samples were obtained to detect the antioxidative enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), and oxidation degradation products of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), conjugated diene (CD), advanced oxidation protein products (AOPP), protein carbonyl (PC), and 8-hydroxydeoxyguanosine (8-OHdG).Results. Serum SOD, GSH-Px, and CAT activities in cataract group were significantly decreased as compared to the control subjects (P<0.05). The levels of MDA, 4-HNE, and CD in cataract patients were significantly higher than those in the control subjects (P<0.05,P<0.01). Cataract patients had higher levels of 8-OHdG, AOPP, and PC with respect to the comparative group of normal subjects (P<0.01). And there was no statistical significance in concentration of antioxidative enzymes and oxidative stress products in patients with different subtype cataract.Conclusions. Oxidative stress is an important risk factor in the development of age-related cataract, and augmentation of the antioxidant defence systems may be of benefit to prevent or delay cataractogenesis.

Effects of exogenous vitamins A, C, and E and NADH supplementation on proliferation, cytokines release, and cell redox status of lymphocytes from healthy aged subjects

2017 ◽  
Vol 42 (6) ◽  
pp. 579-587 ◽  
Author(s):  
Samia Bouamama ◽  
Hafida Merzouk ◽  
Amel Medjdoub ◽  
Amel Merzouk-Saidi ◽  
Sid Ahmed Merzouk
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Vitamin E ◽  
Vitamin C ◽  
The Elderly ◽  
Redox Status ◽  
Age Related ◽  
Immune Alterations ◽  
Cell Redox Status

Aging is an inevitable biological event that is associated with immune alterations. These alterations are related to increased cellular oxidative stress and micronutrient deficiency. Antioxidant supplementation could improve these age-related abnormalities. The aim of this study was to determine in vitro effects of vitamin A, vitamin C, vitamin E, and nicotinamide adenine dinucleotide (NADH) on T cell proliferation, cytokine release, and cell redox status in the elderly compared with young adults. Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were cultured in vitro and stimulated with concanavalin A in the presence or absence of vitamins. Cell proliferation was determined by conducting MTT assays, and based on interleukin-2 and interleukin-4 secretions. Cell oxidant/antioxidant balance was assessed by assaying reduced glutathione (GSH), malondialdehyde, carbonyl protein levels, and catalase activity. The present study demonstrated that T-lymphocyte proliferation was decreased with aging and was associated with cytokine secretion alterations, GSH depletion, and intracellular oxidative stress. In the elderly, vitamin C, vitamin E, and NADH significantly improved lymphocyte proliferation and mitigated cellular oxidative stress, whereas vitamin A did not affect cell proliferation or cell redox status. In conclusion, vitamin C, vitamin E, and NADH supplementation improved T-lymphocytes response in the elderly, and could contribute to the prevention of age-related immune alterations. Consumption of food items containing these vitamins is recommended, and further investigation is necessary to evaluate the effect of vitamin supplementation in vivo.

scholarly journals Oxidative stress and antioxidant vitamins in cataract patients

2019 ◽  
Vol 7 (5) ◽  
pp. 1568
Author(s):  
Ghazala . ◽  
Syed Liaquat Ali ◽  
Jamil Ahmed Siddiqui ◽  
Aziza Khanam ◽  
Saleh Memon
Keyword(s):  
Oxidative Stress ◽  
Demographic Data ◽  
Antioxidant Vitamins ◽  
Blood Levels ◽  
Contributory Factor ◽  
Antioxidant Vitamin ◽  
Diabetic Cataract ◽  
Age Related ◽  
Significant Difference ◽  
Cataract Patients

Background: In Pakistan age related vision disturbances are mainly due to cataract. Various studies have reported relationship of ocular lesion with senile changes and diabetes mellitus resulting in reduced quality of life due to vision. Oxidative stress is an important factor in the process of cataractogenesis. The pathogenesis of the cataract may involve decreased activity of antioxidant scavenging system which includes non-enzymatic natural antioxidants as biomolecules such as carotenoids and vitamins. So, it is planned to investigate the level of serum antioxidant vitamins in diabetic cataract patients and in non-diabetic cataract patients.Methods: The study was conducted at Biochemistry department, Al-Tibri Medical College Karachi from October 2016 to October 2017. Ninety pre diagnosed cataract patients were selected from Al-Ibrahim Eye Hospital Karachi 40 normal control subjects were selected from the same population with same socioeconomic group. The demographic data was analyzed. The random blood sugar, antioxidant vitamins (C, A and E) and malondialdehyde were analyzed in the blood sample of control and cataract patients. The data was analyzed by SPSS version 20.Results: There was no significant difference in the level of vitamin C, A, E and MDA between diabetic and non-diabetic cataract patients, but the blood levels of vitamins of control are higher as compared to the cataract patients. The level of MDA is significantly high in cataract patients as compared to control. Antioxidant vitamin E was negatively correlated with serum malondialdehyde in cataract patients.Conclusions: It is concluded that in diabetic and non-diabetic cataract low level of serum antioxidant vitamins may be a contributory factor for cataractogenesis.

Effects of Vitamin E, C and D Supplementation on Inflammation and Oxidative Stress in Streptozotocin-Induced Diabetic Mice

2013 ◽  
Vol 83 (3) ◽  
pp. 168-175 ◽  
Author(s):  
Agnieszka Greń
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Diabetic Mice ◽  
Concurrent Administration ◽  
Diabetes Group ◽  
C 50 ◽  
And Oxidative Stress ◽  
Preventive Role

Background: The main objective of the study was to analyze the potential ability of vitamins E, C, and D, used as nutritional supplements, in averting inflammation and oxidative stress in the course of diabetes mellitus. Methods: Male mice were divided into eight groups. Diabetes was induced (groups II, VI, VII, and VIII) by an intraperitoneal injection of streptozotocin (STZ). The third and sixth groups were given vitamin C (50 mg/kg) 3 times per week, the fourth and seventh groups were given vitamin E (300 mg/kg) 3 times per week, and the fifth and eight groups were given vitamin D (2000 IU/day). Interleukin-6 levels were measured in serum. Glutathione (GSH) levels and glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) activity were measured in the liver tissue. Results: STZ resulted in a significant decrease in all tested enzymes and glutathione levels, and an increase in IL-6 level in comparison to control animals (p < 0.05). Mice treated with vitamins had a significantly (p < 0.05) higher content of enzymes and glutathione in liver than diabetic mice, however IL-6 concentration showed a significant decrease. Concurrent administration of STZ and vitamins caused a significant increase (compared to the diabetes group) in SOD, CAT, GPx, GSH content, and a decrease in IL-6 levels (p < 0.05). Conclusion: These results indicate the preventive role of vitamin C, E, and D against STZ-induced diabetic oxidative stress and inflammation. Hence these vitamins could be used as an adjuvant therapy for the prevention and/or management of diabetes.

scholarly journals The Effect of Combined Vitamin C and Vitamin E Supplementation on Oxidative Stress Markers in Women with Endometriosis: A Randomized, Triple-Blind Placebo-Controlled Clinical Trial

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Leila Amini ◽  
Razieh Chekini ◽  
Mohammad Reza Nateghi ◽  
Hamid Haghani ◽  
Tannaz Jamialahmadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Clinical Trial ◽  
Vitamin E ◽  
Vitamin C ◽  
Pelvic Pain ◽  
Controlled Clinical Trial ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Group B

Background. Endometriosis is a chronic and estrogen-dependent pelvic inflammatory disease, which may have various causes, such as oxidative stress. Dysmenorrhea, dyspareunia, and pelvic pain are well-known symptoms of endometriosis. The present clinical trial assessed the role of supplementation with antioxidant vitamins on the indices of oxidative stress as well as the severity of pain in women with endometriosis. Materials and Methods. We enrolled 60 reproductive-aged (15–45 years) women with pelvic pain in this triple-blind clinical trial. They had 1–3 stages of laparoscopic-proven endometriosis. The participants were randomized to group A (n = 30), given vitamin C (1000 mg/day, 2 tablets of 500 mg each) and vitamin E (800 IU/day, 2 tablets of 400 IU each) combination, or group B (n = 30), given placebo pills daily for 8 weeks. Results. Following treatment with vitamin C and vitamin E, we found a significant reduction in MDA and ROS compared with the placebo group. There was no significant decline in total antioxidant capacity after treatment. However, the severity of pelvic pain ( p value <0.001), dysmenorrhea ( p value <0.001), and dyspareunia ( p value <0.001) significantly decreased in the treatment group after 8 weeks of supplementation. Conclusions. The present findings support the potential role of antioxidants in the management of endometriosis. The intake of vitamin C and vitamin E supplements effectively reduced dysmenorrhea severity and improved dyspareunia and severity of pelvic pain.

Role of Oxidative Stress and Mitochondrial Dysfunction in Skeletal Muscle in Type 2 Diabetic Patients

2016 ◽  
Vol 22 (18) ◽  
pp. 2650-2656 ◽  
Author(s):  
Noelia Diaz-Morales ◽  
Susana Rovira-Llopis ◽  
Irene Escribano-Lopez ◽  
Celia Bañuls ◽  
Sandra Lopez-Domenech ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Mitochondrial Dysfunction ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

scholarly journals FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rawshan Choudhury ◽  
Nadhim Bayatti ◽  
Richard Scharff ◽  
Ewa Szula ◽  
Viranga Tilakaratna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Fate ◽  
Retinal Pigment ◽  
Factor H ◽  
Age Related Macular Degeneration ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Rpe Cells ◽  
Age Related

AbstractRetinal pigment epithelial (RPE) cells that underlie the neurosensory retina are essential for the maintenance of photoreceptor cells and hence vision. Interactions between the RPE and their basement membrane, i.e. the inner layer of Bruch’s membrane, are essential for RPE cell health and function, but the signals induced by Bruch’s membrane engagement, and their contributions to RPE cell fate determination remain poorly defined. Here, we studied the functional role of the soluble complement regulator and component of Bruch’s membrane, Factor H-like protein 1 (FHL-1). Human primary RPE cells adhered to FHL-1 in a manner that was eliminated by either mutagenesis of the integrin-binding RGD motif in FHL-1 or by using competing antibodies directed against the α5 and β1 integrin subunits. These short-term experiments reveal an immediate protein-integrin interaction that were obtained from primary RPE cells and replicated using the hTERT-RPE1 cell line. Separate, longer term experiments utilising RNAseq analysis of hTERT-RPE1 cells bound to FHL-1, showed an increased expression of the heat-shock protein genes HSPA6, CRYAB, HSPA1A and HSPA1B when compared to cells bound to fibronectin (FN) or laminin (LA). Pathway analysis implicated changes in EIF2 signalling, the unfolded protein response, and mineralocorticoid receptor signalling as putative pathways. Subsequent cell survival assays using H2O2 to induce oxidative stress-induced cell death suggest hTERT-RPE1 cells had significantly greater protection when bound to FHL-1 or LA compared to plastic or FN. These data show a non-canonical role of FHL-1 in protecting RPE cells against oxidative stress and identifies a novel interaction that has implications for ocular diseases such as age-related macular degeneration.

scholarly journals Age-Related Macular Degeneration: Role of Oxidative Stress and Blood Vessels

2021 ◽  
Vol 22 (3) ◽  
pp. 1296
Author(s):  
Yue Ruan ◽  
Subao Jiang ◽  
Adrian Gericke
Keyword(s):  
Oxidative Stress ◽  
Macular Degeneration ◽  
Vascular Function ◽  
Vascular Dysfunction ◽  
Therapeutic Strategies ◽  
Vision Impairment ◽  
Age Related Macular Degeneration ◽  
Oxygen Species ◽  
Age Related

Age-related macular degeneration (AMD) is a common irreversible ocular disease characterized by vision impairment among older people. Many risk factors are related to AMD and interact with each other in its pathogenesis. Notably, oxidative stress and choroidal vascular dysfunction were suggested to be critically involved in AMD pathogenesis. In this review, we give an overview on the factors contributing to the pathophysiology of this multifactorial disease and discuss the role of reactive oxygen species and vascular function in more detail. Moreover, we give an overview on therapeutic strategies for patients suffering from AMD.

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