scholarly journals Diagnosis of tubercular lymphadenopathy by fine needle aspiration cytology and Z-N staining

2019 ◽  
Vol 7 (8) ◽  
pp. 2985
Author(s):  
Navneet Naz ◽  
Megha Sharma
Keyword(s):  
Extrapulmonary Tuberculosis ◽  
Diagnostic Technique ◽  
Needle Aspiration ◽  
Vital Role ◽  
Cervical Region ◽  
Medical College ◽  
Caseous Necrosis ◽  
Needle Aspiration Cytology ◽  
Minimal Invasion ◽  
Tubercular Lymphadenitis

Background: Tuberculosis continues to be the biggest health problem in India. Tuberculosis involves respiratory, gastrointestinal tract as well as extrapulmonary site. Tubercular lymphadenopathy is the most common form of extrapulmonary tuberculosis. FNAC plays a vital role in diagnosis of tubercular lymphadenopathy. FNAC is not only used for cytological diagnosis but also used for other ancillary tests like Ziehl-Neelsen staining and AFB culture.Methods: The study was conducted in the department of pathology, Government Medical College, Jammu over a period of 6 months and included 450 cases presenting with superficial lymphadenopathy. FNAC was performed in the cases and smears in each case, were stained with May Grunwald Giemsa (MGG), Papanicolaou and Z-N stain.Results: Out of 450 cases,160 cases (35.5%) showed features of tubercular lymphadenitis. The most common site of presentation, being cervical region with 75% cases. Females outnumbered males by ratio of 1.46:1. In cytomorphology 93 cases (58.1%) showed epithelioid granulomas with caseous necrosis,37 cases (23.1%) showed caseous necrosis only while only epithelioid granulomas were seen in 30 cases (23.1%). AFB positivity was seen in 82 cases with maximum AFB positivity (78.3%) seen in cases with necrosis only.Conclusions: FNAC is a rapid diagnostic technique because of its simplicity, cost effectiveness, early availability of results and minimal invasion. FNAC is a reliable and sensitive first line investigation in diagnosis of tubercular lymphadenitis combined with AFB staining.

scholarly journals Correlation between cytomorphology and acid fast bacilli positivity in tubercular lymphadenopathy in far Western Nepal: a hospital based study

2020 ◽  
Vol 8 (8) ◽  
pp. 2933
Author(s):  
Ashok Shreepaili ◽  
Sushil Dhakal
Keyword(s):  
Lymph Nodes ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Tuberculous Lymphadenitis ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Caseous Necrosis ◽  
Needle Aspiration Cytology ◽  
Tubercular Lymphadenitis

Background: Tuberculous lymphadenitis is the most common manifestation of extrapulmonary tuberculosis. Fine needle aspiration cytology (FNAC) is a valuable tool in diagnosis of tubercular lymphadenitis. The present study aims to determine the prevalence of different cytomorphological patterns in fine needle aspiration cytology of tubercular lymph nodes and their correlation with Acid Fast Bacilli (AFB) positivity on Ziehi-Neelsen (ZN) Staining.Methods: Fine needle aspiration cytology of 274 cases diagnosed as tubercular lymphadenitis over a period of a year were reviewed. Cytomorphological patterns were categorized into three patterns. Pattern I: Epithelioid granuloma without necrosis, Pattern II: Epithelioid granuloma with caseous necrosis and Pattern III: Caseous necrosis only. Fischer Exact test was applied to correlate cytomorphological pattern and AFB positivity.Results: Tuberculous lymphadenitis was most frequent in age group 21-30 years (24.81%). Cervical lymph nodes were the most frequent lymph nodes involved (84.67%). Pattern I was the most common cytomorphological pattern observed (49.3%). Overall AFB positivity was 28.10%. Maximum AFB positivity was seen in pattern III (73.3%).Conclusions: FNAC is the simple, cost effective and minimally invasive tool to diagnose tuberculous lymphadenitis. Study of both cytomorphological pattern and ZN staining for AFB can improve the diagnostic accuracy.

scholarly journals Patterns of lymphadenopathy on fine needle aspiration cytology in eastern Nepal

2015 ◽  
Vol 5 (10) ◽  
pp. 814-816
Author(s):  
N Mainali ◽  
RB Suwal
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Clinical Problem ◽  
Needle Aspiration ◽  
Cervical Region ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Common Diagnosis ◽  
Needle Aspiration Cytology ◽  
Tubercular Lymphadenitis

BACKGROUND: Lymphadenopathy is a commonly encountered clinical problem. Fine needle aspiration cytology offers the alternative of an immediate, preliminary, although not always specific diagnosis with little trauma and cost, thus providing ample information for further management and reduce the amount of open biopsy.MATERIAL AND METHODS:  FNAC was performed using 21 gauge needle attached with 10ml disposable needle. Slides were prepared, two were air dried and stained with field stain where as two were fixed in ethanol and was stained with PAP stain. One slide was stained with Ziehl-Neelson stain. Field and PAP stained slide were reviewed and diagnosed by Pathologist.RESULT: Out of the 225 patients, reactive lymphadenitis was the most common diagnosis (54.2%), followed by tubercular lymphadenitis (33.44%). Among the age group percentage of tubercular lymphadenitis was more in 210-30 years of age.  Majority of the metastatic carcinoma was in cervical region, comprising mainly squamous cell carcinoma.CONCLUSION: Reactive lymphadenitis was the most common cause of lymphadenopathy especially in case of children. In case of older population, definite pathology for lymph node enlargement was found in most of the cases. Hence FNAC is warranted in lymphadenopathy, as it is simple yet of great diagnostic value.

scholarly journals Cytomorphological Spectrums in Tuberculous Lymphadenitis: Understanding the Stages of Disease

2018 ◽  
Vol 1 (2) ◽  
pp. 21-29
Author(s):  
Anju Pradhan ◽  
P. Poudyal ◽  
P. Upadhyaya ◽  
S. Pokhrel
Keyword(s):  
Lymph Nodes ◽  
Chronic Renal Disease ◽  
Extrapulmonary Tuberculosis ◽  
Previous History ◽  
Diagnostic Technique ◽  
Neck Region ◽  
Needle Aspiration ◽  
Tuberculous Lymphadenitis ◽  
Granulomatous Lymphadenitis ◽  
Needle Aspiration Cytology

Introduction: Tuberculous lymphadenitis is the most common form of extrapulmonary tuberculosis and one of the main causes of lymphadenopathy. Fine Needle Aspiration Cytology (FNAC) has played a substantial role in diagnosis of tuberculous lymphadenopathy and it has become a first-line diagnostic technique. The aim of this study was to describe and understand the spectrum of cytomorphological changes seen in tuberculous lymphadenitis corresponding to stages of disease and to associate the cytomorphological changes with Acid Fast Bacilli (AFB) positivity. Methods: This is a retrospective cytomorphological study of cytologically diagnosed tuberculous lymphadenitis. The recorded details of clinical presentation and site of the lymph nodes were noted. The slides were stained with Papanicolaou, Giemsa and AFB. Result: There were a total of 203 cases with age ranging from 3- 75 years. Four cytomorphological patterns observed were Necrotising Granulomatous Lymphadenitis (NGL; 45.32%), Granulomatous Lymphadenitis (GL, 18.22%), Necrotising Suppurative Lymphadenitis (NSL; 21.18%) and Necrotising Lymphadenitis (NL; 15.27%). Necrotising patterns were observed in immunocompromised individuals like HIV infected patients, in patients with previous history of tuberculosis and in patients with chronic renal disease. Strong AFB positivity was observed in necrotising patterns. Lymph nodes of head and neck region were the most common site of involvement with cervical being the commonest. Conclusion: Necrotizing patterns are observed in the later stages of disease or in the immunocompromised patients. Strong positivity for AFB is observed in the smears with necrotizing patterns and less in the granulomatous pattern.

“CYTOMORPHOLOGICAL PATTERNS OF TUBERCULOUS LYMPHADENITIS AND ITS CORRELATION WITH ZIEHL-NEELSEN STAINING AND CBNAAT IN A TERTIARY CARE CENTRE OF HARYANA”

2021 ◽  
pp. 73-75
Author(s):  
Singh S ◽  
Arora S ◽  
Munesh Munesh ◽  
Mahajan S
Keyword(s):  
Developing Countries ◽  
Tertiary Care ◽  
Diagnostic Technique ◽  
Needle Aspiration ◽  
Tuberculous Lymphadenitis ◽  
Tertiary Care Centre ◽  
Adequate Treatment ◽  
Caseous Necrosis ◽  
Cytological Features ◽  
Needle Aspiration Cytology

Introducion: Tuberculosis (TB) is still prevalent in developing countries, and one of the most common cause of tuberculous lymphadenopathy. Quick diagnosis is necessary for adequate treatment. Fine needle aspiration cytology (FNAC) is an efcient and rapid primary diagnostic technique as an alternative to excision. Cytomorphologic features of well-formed epithelioid granulomas and the presence of caseous necrosis are sufcient for cytological diagnosis. Mycobacteriological conrmation however, is essential to rule out other causes of granulomatous inammation. Material and methods: This study was aimed at evaluating and correlating the role of FNAC, mycobacterium recognition by Ziehl-Neelsen staining (ZN staining) with Cartridge based nucleic acid amplication test (CBNAAT) positivity to diagnose tuberculous lymphadenitis. FNA material was collected from 97 patients and was analysed by cytomorphology, ZN stain and CBNAAT. Result: Out of 97 cases, 66 cases had cytological features consistent with TB. 40.9% of the smears showed epithelioid granulomas with caseous necrosis, followed by 31.8% smears showing epithelioid granuloma without necrosis and necrosis with polymorphs without granuloma in 27.3% of the smears. Amongst the 27 cases showing granuloma with necrosis, there was 44.4% AFB positivity and 48.1% CBNAAT positivity while 21 cases of granuloma without necrosis showed 52.3% AFB positivity and 47.6% CBNAAT positivity. In the 18 cases showing necrosis there was 72.2% AFB positivity and 83.3% CBNAATpositivity. Conclusion: The presence of caseous necrosis and granulomas are strongly suggestive of tubercular etiology, especially in developing countries with high incidence of TB. AFB smear CBNAATcan supplement cytomorphometry for rapid and accurate diagnosis.

Role of fine needle aspiration cytology in the preoperative diagnosis of malignancy in parotid and submandibular gland neoplasm

2014 ◽  
Vol 19 (2) ◽  
pp. 110-118
Author(s):  
Mohammad Amzad Hossain ◽  
Md Zahedul Alam ◽  
Md Rojibul Haque ◽  
Md Nazmul Haque ◽  
KM Nurul Alam ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
False Negative ◽  
Needle Aspiration ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Medical College ◽  
Preoperative Diagnostic ◽  
Needle Aspiration Cytology

Objective: To evaluate the role of Fine Needle Aspiration Cytology in the preoperative diagnosis of malignancy in parotid and submandibular gland neoplasm. Methods: This cross sectional study on 50 cases was conducted in the Department of Otolaryngology and Head-Neck Surgery of Sir Salimullah Medical College Mitford Hospital and Dhaka Medical College Hospital from January’2009 to June 2010. Results: Fine needle aspiration cytology findings of our all 50 cases were compared with postoperative histopathological reports. Out of the 50 cases, in 36(72%) cases of benign neoplasm and 7(14%) cases of malignant neoplasm, pre-operative FNAC findings and post operative histopathological findings were same. In 7 cases, FNAC and post operative histopathological findings did not matched. These were 2 (4%) false positive and 5 (10%) false negative result.In our study sensitivity of FNAC for reporting malignancy was 58.33%, specificity to rule out malignancy was 94.73% and overall accuracy in detecting malignant tumour was 86%. Positive predictive value and negative value were 77.77% and 87.80% respectively. It can be concluded that fine needle aspiration cytology is a safe, cheap and useful preoperative diagnostic tool in the diagnosis of malignancy in parotid and submandibular gland, but as fine needle aspiration cytology partly depends on operator skill, it may give false negative and false positive result. Conclusion: FNAC is a useful preoperative diagnostic tool for malignant parotid and submandibular glands with high specificity and sensitivity. DOI: http://dx.doi.org/10.3329/bjo.v19i2.17634 Bangladesh J Otorhinolaryngol 2013; 19(2): 110-118

A Study on Clinical Evaluation and Management Modalities in Benign Breast Diseases

2021 ◽  
Vol 8 (03) ◽  
pp. 168-171
Author(s):  
Ajay Chanakya V ◽  
Sreekanth Kotagiri ◽  
Gogineni Tarun Chowdary ◽  
Sriram K.V.V.S.D. ◽  
Ch. Vasu Reddy ◽  
...  
Keyword(s):  
Surgical Oncology ◽  
Needle Aspiration ◽  
Breast Diseases ◽  
Study Group ◽  
Psychological Burden ◽  
Diagnosis And Management ◽  
Medical College ◽  
Benign Breast Diseases ◽  
Needle Aspiration Cytology ◽  
A Prospective Study

BACKGROUND Benign breast diseases (BBDs) are a common cause of breast problems in up to 30 % of women. The pathogenesis of BBD involves disturbances in the breast physiology. There is a necessity for thorough comprehensive, prompt diagnosis and management of the benign conditions to reduce the physical and psychological illness of the patients who are anxious about the possibility of the disease being a malignant one. METHODS This was a prospective study conducted in the Department of Surgical Oncology, GSL medical college from October 2016 to July 2018. Females presenting with breast lump, confirmed with fine needle aspiration cytology (FNAC), aged between 12 – 55 years and fit for surgery were included in the study. Those who did not submit the informed consent were excluded. Statistical analysis was performed by SPSS version-21 and MS Excel 2013. RESULTS Most (74 %) of the patients were between 20 and 40 years of age, 56 % were diagnosed to be fibroadenoma. In the study group, 82 % (41) participants with BBD were married. The most common presenting complaint in the study group was lump (50 %) in the breast followed by lump associated with pain (28 %). Patients with fibroadenoma mass had well defined features. CONCLUSIONS Lump was the most common clinical presentation followed by lump with pain. Based on the FNAC report, decision is made for management. Comprehensive and prompt preoperative diagnosis and management of the benign conditions is necessary to reduce the physical and psychological burden on the patients who are anxious about the possibility of malignancy. KEYWORDS Breast, Participants, Study, Age

TO STUDY THE CYTOMORPHOLOGICAL PATTERNS OF TUBERCULAR LYMPHADENOPATHY AND ITS HISTOPATHOLOGICAL CORRELATION

2021 ◽  
pp. 26-27
Author(s):  
Rashmi Sharma ◽  
Poonum Gogania ◽  
Geeta Pachori ◽  
Akhilesh Bharadwaj
Keyword(s):  
Epithelioid Cell ◽  
Cervical Region ◽  
Tuberculous Lymphadenitis ◽  
Female Ratio ◽  
Caseous Necrosis ◽  
Histopathological Correlation ◽  
Histopathological Evaluation ◽  
Special Stain ◽  
Hematoxylin And Eosin ◽  
Tubercular Lymphadenitis

INTRODUCTION: Tubercular lymphadenopathy is one of the common clinical problems. Practically diagnosis of tuberculosis sometimes creates a challenge to the pathologist and clinician. A combined approach of cytology with aid of special stain and histopathological evaluation helps to conrm the diagnosis. AIM -The present study was carried out to evaluate the different cytological patterns of tuberculous lymphadenitis along with utility of special stain like Acid fast stain and further histopathological evaluation. MATERIAL AND METHOD: Smears from 833 cytologically diagnosed cases of tubercular lymphadenitis were prepared and stained with Hematoxylin and Eosin (H&E), Giemsa and Acid-fast stain. All the smears were categorized into four cytomorphological patterns and correlated with 250 histopathologically available cases. RESULT: Tuberculosis is the major cause of lymphadenopathy. Majority of cases (63 %) were in their second to third decades of life, with male to female ratio of 1:1.4. Cervical region was the most common site of involvement (83.5 %). Smear revealed epithelioid granulomas with caseous necrosis in maximum cases (46.9 %). AFB positivity was seen highest in smear revealing necrosis only with or without epithelioid cell (93,8 %). histopathological correlation was seen in 248 cases out of 250 available cases. CONCLUSION: FNAC has been proved very safe, highly sensitive, and rst line investigation in diagnosing tubercular lymphadenitis. The approach to tubercular lymphadenitis attains completeness with cytopathological, Acid Fast stain and histopathological evaluation.

scholarly journals Tuberculosis Infection Mimicking Brain Metastatic Malignancy Lesions in an Elderly Male

2013 ◽  
Vol 2013 ◽  
pp. 1-3
Author(s):  
Dimitrios Anyfantakis ◽  
Ageliki Damianaki ◽  
Maria Kokosi ◽  
Emmanouil K. Symvoulakis ◽  
Serafim Kastanakis
Keyword(s):  
Medical Condition ◽  
Extrapulmonary Tuberculosis ◽  
Needle Aspiration ◽  
Axillary Lymph ◽  
Elderly Male ◽  
Perivascular Edema ◽  
Metastatic Lesions ◽  
Granulomatous Lymphadenitis ◽  
Nodular Lesions ◽  
Needle Aspiration Cytology

An 83-year-old Caucasian Greek man was referred by his general practitioner to the emergency department of the general hospital in Crete because of seizures and agitation. His past medical history was negative for any neurological or medical condition. Electroencephalogram showed a bradyarrhythmic theta activity, without evidence of any focal or other specific abnormality. Magnetic resonance imaging of the brain demonstrated a number of diffuse nodular lesions and moderate perivascular edema. An axillary lymph node fine needle aspiration cytology suggested a granulomatous lymphadenitis along with signs of tuberculous infiltration. Tuberculin skin test was positive. We report a rare case of extrapulmonary tuberculosis mimicking brain metastatic lesions.

Cytopathological Evaluation of Thyroid Lesions Based on Bethesda System in Patients Attending Birat Medical College and Teaching Hospital

2020 ◽  
Vol 22 (4) ◽  
pp. 260-265
Author(s):  
Neeta Kafle ◽  
B Koirala ◽  
SU Kafle ◽  
M Singh ◽  
A Sinha
Keyword(s):  
Teaching Hospital ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Medical College ◽  
Bethesda System ◽  
Needle Aspiration Cytology ◽  
Thyroid Lesions

More than 50% of the world’s population has at least a thyroid nodule. Detail clinical examination and radiology may help in diagnosing thyroid lesions but the management depends upon the cytopathological diagnosis. Optimum use of fine needle aspiration cytology (FNAC) and better understanding of cytomorphological characteristic of thyroid lesions by using Bethesda system, triaging of patients who are to be treated medically or surgically is more accurate. The objective of this present study is cytopathological evaluation of thyroid lesions based on Bethesda System in patients attending Birat Medical College and Teaching Hospital. The objective was also to correlate the cytological findings with histopathological findings where ever possible. A total of 104 patients with thyroid lesions underwent fine needle aspiration cytology in a period of a year (September 1, 2019 to August 31, 2020). Cytological features were evaluated and classified according to the Bethesda system. Histopathological features were evaluated and correlated wherever available. Among 104 patients with thyroid lesions 93 were female and 11 were male. Four cases turned out to be non diagnostic, 85 benign, three Atypia of undetermined significance, three Suspicious for follicular neoplasm and eight Suspicious of malignancy and one Malignant according to Bethesda system. Histopathology specimen was received in 31 patients out of whom 20 (64.5%) patients were reported as colloid nodule, two follicular adenoma, one Hurthle cell adenoma, six papillary carcinoma and two follicular carcinoma. Medullary carcinoma and anaplastic carcinoma were not seen in the patients evaluated. Specificity and sensitivity of fine needle aspiration cytology was 94.7% and 88.9% respectively. Thus reporting thyroid lesions FNAC with Bethesda system allow a more specific cytological diagnosis.

scholarly journals Hydatid Cyst of Breast: A Case Report

2015 ◽  
Vol 12 (1) ◽  
pp. 41-42
Author(s):  
F Alam ◽  
RG Goel
Keyword(s):  
Case Report ◽  
Hydatid Cyst ◽  
Hydatid Disease ◽  
Needle Aspiration ◽  
Accurate Information ◽  
Endemic Region ◽  
Medical College ◽  
Control And Prevention ◽  
History Of ◽  
Needle Aspiration Cytology

Hydatid cyst is a zoonotic disease which occurs due to infectivity with larval stage of Echinococcus granulosus. The disease is chronic and cysts can be lodged in different organs. It has cosmopolitan distribution and impact health and economical challenges for many countries throughout the world. The location of the disease is mostly in the liver and lungs. Hydatid disease of breast is extremely rare. A case report of 24 year female with the diagnosis of the hydatid breast is described in detail. The patient presented with painless lump in the upper inner quadrant of right breast of two months duration. History of trauma, pus discharge, itching, weight loss, fever or hormonal therapy were absent. The patient was diagnosed preoperatively as cysticercosis right breast by fine needle aspiration cytology based on the laboratory results conducted outside the hospital. However, histopathology confirmed the diagnosis of hydatid breast. It is the second diagnosed case in Nepal. Therefore, accurate information on the distribution of the disease is first step for the control and prevention. Only few reports are published in the literature about breast hydatid cyst.Thus, we want to emphasize the importance of keeping hydatid disease in differential diagnosis of cystic breast lesions, particularly in endemic region, where the disease could mimic fibroadenoma, phyllodes tumors, chronic abscesses.Journal of Nepalgunj Medical College Vol.12(1) 2014: 41-42

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