scholarly journals Biofilm production and its correlation with antibiogram among clinical isolates of uropathogenic Escherichia coli

2018 ◽  
Vol 5 (3) ◽  
pp. 638
Author(s):  
Monika Yadav ◽  
Sulochana D. Khumanthem ◽  
Mamta D. Kshetrimayum ◽  
Shan Damrolien
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Health Problem ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Isolation And Identification ◽  
Biofilm Production ◽  
Antibiotic Susceptibility Test

Background: Urinary tract infection (UTI) is one of the commonest health problem encountered in clinical practice. The biofilms play major role in decreasing the susceptibility to the antimicrobial agents. The present study aimed at isolation and identification of Escherichia coli from cases of urinary tract infection, and to find out production of biofilms by these isolates and to correlate it with antimicrobial resistance.Methods: This study was a cross sectional study. Urine specimen from patients suspected of UTIs were collected and processed by standard operative procedure, antibiotic susceptibility test was done by Kirby Bauer disc diffusion method. Biofilm production in isolates was done by Tissue culture plate (TCP) method, Christensen’s test tube (CTT) method, and Congo red agar (CRA) method.Results: Out of the total of 250 isolates 191 (76%) showed biofilm positive by TCP method, 180 (72%) showed biofilm positive by CTT method, 161 (64%) showed biofilm positive by CRA method. 90% of total extended spectrum beta lactamase (ESBL) producing Escherichia coli were biofilm producers. There is more resistance for each and every antibiotic among biofilm producers in comparision to biofilm non producers.Conclusions: The microbial biofilms may pose a public health problem, as the microorganisms in the biofilms are difficult to be treated with antimicrobial agents.

Prevalence of Fluroquinolone and Third-Generation Cephalosporin Resistance among the Patients With Urinary Tract Infection Due to Escherichia Coli

2021 ◽  
Vol 11 (Number 1) ◽  
pp. 26-32
Author(s):  
Chowdhury MJ ◽  
Faruque CMO ◽  
Noor J ◽  
Rouf CM ◽  
Hossain MM ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Bacterial Infections ◽  
Generation Cephalosporin ◽  
Diffusion Method ◽  
Third Generation ◽  
Isolation And Identification ◽  
Cross Sectional

Background: Urinary tract infection (UTI) has become the most frequent bacterial infections worldwide. It is well established that Escherichia coli is the predominant cause of UTI. The aim of our study was to evaluate the rates of resistance to fluroquinolone and third generation cephalosporin among the patients with UTI due to E.Coli and to assess the potential correlation between both trends. Methods: The study was a cross sectional observational study conducted at the Department of Pharmacology and Therapeutics in collaboration with Department of Microbiology of Sylhet Women’s Medical College and Hospital from 1st July 2019 to 30th June 2020. Results: A total of 246 urine samples were collected from patients with UTI followed by isolation and identification of E.coli strains. Antibiotic sensitivity and resistance analysis was performed by the disc diffusion method employing multiple antibiotic discs. The sensitivity was monitored by zone of inhibition around the disc. Overall rates of resistance to fluroquinolone and third generation cephalosporin were 70.31% and 65.10% respectively. The rates of co-resistance to both fluroquinolone and third generation cephalosporin was 53.13%. Conclusion: Our study suggests that fluroquinolone should be reserved and third generation cephalosporin should be used with caution among patients with E.coli.

scholarly journals Antimicrobial Resistance in Uropathogenic Escherichia coli Strains Isolated from Beasat Hospital in Sanandaj, West of Iran

2020 ◽  
pp. 32-36
Author(s):  
Nasrin Bahmani ◽  
Noshin Abdolmaleki ◽  
Afshin Bahmani
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Agents ◽  
Hospitalized Patients ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli

Background and Objectives: Urinary tract infection (UTI) is one of the most frequent infectious diseases which is caused by Gram-negative bacteria especially Escherichia coli. Multiple resistance to antimicrobial agents are increasing quickly in E. coli isolates and may complicate therapeutic strategies for UTI. The propose of this study was to determine the antibiotic resistance patterns and the multidrug-resistance (MDR) phenotypes in uropathogenic E. coli (UPEC). Materials and Methods: A total of 153 UPEC isolates were collected from both hospitalized patients (95 isolates) and outpatients (58 isolates) from March to October 2018. In order to determine the MDR among UPEC isolates, we have tested 15 antimicrobial agents on Muller Hinton agar by the disk diffusion method. Results: The percentage of MDR isolates (resistant to at least three drug classes such as fluoroquinolones, penicillins and cephalosporins) was 55.5% in the hospitalized patients and the outpatients. Antibiotic resistance to ampicillin, ceftazidime, nalidixic acid and trimethoprim/ sulfamethoxazole was higher than 60%. Meropenem, Imipenem and norfloxacin indicated markedly greater activity (93.3%, 80% and 85.6%, respectively) than other antimicrobial agents. Conclusions: Urinary tract infection due to MDR E. coli may be difficult to treat empirically due to high resistance to commonly used antibiotics, so, empirical antibiotic treatment should be reviewed periodically at local studies.

scholarly journals Antibiotic Sensitivity of Bacteria Causing Urinary Tract Infection

2017 ◽  
Vol 2 (1) ◽  
pp. 13-18
Author(s):  
Rezina Parveen ◽  
Md. Abdullah Yusuf ◽  
Ishrat Sharmin ◽  
Md. Saiful Islam ◽  
Ina Rahim
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Agents ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
College Hospital ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Background: Urinary tract infection is very common in both male and female.Objectives: The purpose of the present study was to see the antibiotic sensitivity pattern of isolated from urinary tract infected patients.Methodology: This cross sectional study was carried out in Dhaka Medical College Hospital, Dhaka for a period of 12 months. Clinically diagnosed cases of urinary tract infection irrespective of age and sex having pus cells ?5/HPF in the deposits of centrifuged urine were selected as study population. Data regarding organisms causing UTI and their antibiotic sensitivity patterns were collected. For urine culture the urine samples were inoculated on HiCrome UTI agar, CLED agar, 5% sheep blood agar and MacConkey’s agar media with a calibrated loop having diameter of 1.45 mm which contains 0.001 ml of urine. The inoculation at 37o C for 24 hours and CFU count of 105/ml of urine were considered positive for UTI. Identification of bacteria was done by standard biochemical techniques and their distinct colony characteristics. All the isolated organisms were tested for antimicrobial sensitivity against different antimicrobial agents by disc diffusion method on Mueller-Hinton agar plates.Result: Diagnosis of bacteria causing UTI with their sensitivity to different antibiotics was performed with a total of 300 samples from both male (38.66%) and female (61.33%) of different age groups. Among 300 samples 107 strains were isolated. Out of 107 identified strains, 95(31.67%) samples showed single growth and 6(2%) samples showed mixed growth. Escherichia coli (64.49%) was found to be the predominant organism. Regarding antimicrobial sensitivity pattern Esch. coli showed 98.55 to 63.77% sensitivity to imipenem, amikacin, ceftazidime and nitrofurantoin. Other isolated organisms showed 50 to 100% sensitivity to ceftazidime, amikacin, imipenem except Klebsiella, Pseudomonas and enterococci spp. which showed 40% and less sensitivity.Conclusion: In conclusion Escherichia coli is the most commonly isolated bacteria which is highly sensitive to imipenem.Bangladesh J Infect Dis 2015;2(1):13-18

scholarly journals In-Vitro Biofilm Formation and Antimicrobial Resistance of Escherichia coli in Diabetic and Nondiabetic Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Sunayana Raya ◽  
Ankit Belbase ◽  
Laxmi Dhakal ◽  
Krishna Govinda Prajapati ◽  
Reena Baidya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Diabetic Patients ◽  
Microtitre Plate ◽  
Plate Method ◽  
E Coli ◽  
Biofilm Production

Background. Diabetic patients are more susceptible to urinary tract infection compared to nondiabetic patients, Escherichia coli being the most common uropathogen causing UTI. Unreasonable and incorrect antibiotic prescription for UTI in these patients may induce the development of antibiotic-resistant urinary pathogens resulting in delayed recovery and longer hospitalization. In addition to these, biofilm forming capacity of the pathogen may worsen the problem. The main aim of this cross-sectional study (conducted from March to September 2015) is to detect the biofilm forming capacity of UTI causing micro-organisms and compare the antibiotic resistance pattern of Escherichia coli, the most common cause of UTI, which will help the physician in choosing the best antibiotic. Method. Total of 1,099 clean-catch mid stream urine (CCMSU) was processed by standard microbiological technique; 182 were from the diabetic group and 917 nondiabetic. Following identification, all isolates were subjected to antibiotic susceptibility testing using modified Kirby-Bauer disc diffusion method. In-vitro biofilm forming capacity of the isolates were detected by Microtitre plate method. The data were analyzed using SPSS software 16. Result. Urinary tract infection was found to be significantly higher in diabetic patients (42.9%) compared to nondiabetic patients (17.4%) with Escherichia coli as the most common uropathogen in both diabetic and nondiabetic groups. Similarly, UTI was more common in elderly population (29.5%). Imipenem, nitrofurantoin and amikacin were found to be the most effective drug for uropathogenic E. coli in both diabetic and nondiabetic patients, whereas amoxicillin, ciprofloxacin, and cotrimoxazole were least effective. Of the total bacterial isolates, 43.3% showed positive results for in-vitro biofilm production by the Microtitre plate method. A significantly higher resistance rate was observed among biofilm producing E. coli for quinolones, cotrimoxazole, and third generation cephalosporin ceftriaxone. Most of the biofilm producers (79.5%) were found to be MDR (p-value 0.015). Conclusion. Elderly populations with diabetes are at a higher risk of UTI. Higher biofilm production and resistance to in-use antimicrobial agents in this study render its inefficacy for empirical treatment and point out the importance of biofilm screening to ensure the effective management of infection.

scholarly journals Urinary Tract Infection among Patients Visiting Ganesh Man Singh Memorial Hospital and Research Center, Lalitpur, Nepal

2019 ◽  
Vol 6 ◽  
pp. 108-112
Author(s):  
Janak Raj Dhungana ◽  
Aruna Budhathoki ◽  
Goma Poudel ◽  
Jyotika Basnet ◽  
Ravi Shah
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Sensitivity ◽  
Memorial Hospital ◽  
Diffusion Method ◽  
Gram Negative ◽  
Microbiological Methods ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

Objectives: The objective of this study was to determine the prevalence of urinary tract infection (UTI) and antibiotic sensitivity pattern among the suspected UTI cases visiting at Ganeshman Singh Memorial Hospital Lalitpur, Nepal. Methods: A total of 300 mid-stream urine, catheter and suprapubic aspirate from UTI suspected patients were included and processed for routine microscopy and culture and then identified by standard microbiological methods. Antibiotic susceptibility test was performed by Kirby- Bauer disc diffusion method. Results: Out of 300 samples, 55(84.6%) mid-stream urine and 10(15.4%) catheter sample had significant bacterial growth. E. coli (32,49.2%) was the most common isolate followed by Staphylococcus aureus (10,15.3%), Enterobacter spp. (8,12.3%), Klebsiella spp. (7,10.7%), Pseudomonas aeruginosa (3,4.6%), Proteus spp.  (3,4.6%), Acinetobacter spp. (1,1.5%) and Enterococcus spp. (1,1.5%). Most of the Gram-negative bacterial isolates were sensitive to Ceftriaxone (88.8%) followed by Gentamicin (72.2%), and Nitrofurantoin (64.8%) and resistant to Amoxicilin (68.5%) followed by Nalidixic Acid (53.7%). Gram positive isolates were sensitive to Amikacin (72.7%) followed by Imipenem (63.6%) and Gentamicin (63.6%) whereas resistant to Amoxycilin (72.7%) and Ciprofloxacin (63.63%). Conclusion: The main cause of the UTIs was found as Gram negative bacteria. Prescription of antibiotics based on susceptibility tests would help in reduction of antibiotic resistance.

scholarly journals Prevalence of blaCTX-M, blaSHV, and blaTEM Genes in Escherichia coli Strains Isolated From Urinary Tract Infection Samples of Patients in the Intensive Care Unit in Qom, Iran

2020 ◽  
Vol 7 (2) ◽  
Author(s):  
Shima Sadat Lesani ◽  
Mohammad Soleimani ◽  
Pegah Shakib ◽  
Mohammad Reza Zolfaghari
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Urinary Tract Infection ◽  
Intensive Care ◽  
Urinary Tract ◽  
Tract Infection ◽  
Intensive Care Units ◽  
Diffusion Method ◽  
E Coli ◽  
Tract Infections

Background: Escherichia coli is considered as one of the causes of opportunistic infections. Nowadays, due to the increase in drug resistance, the treatment of these infections has become very difficult and they are recognized as the main causes of death in hospitalized patients. Objectives: The aim of this study was to determine the prevalence of blaTEM, blaSHV, and blaCTX-M genes in E. coli strains isolated from the urinary tract infection in patients in Intensive Care Units of three different hospitals in Qom, Iran. Methods: This study was conducted in three months from October to December 2014. A total of 200 E. coli samples were taken from the patients with urinary tract infections in Intensive Care units of Qom hospital. The disc diffusion method was used to determine the susceptibility pattern of antibiotic and phenotypic confirmatory tests for screening of the expanded spectrum beta-lactamase (ESBL) isolates. The presence of blaTEM, blaSHV, and blaCTX-M genes was evaluated by the polymerase chain reaction (PCR) assay. Results: Of 200 samples, ampicillin (96%) and nitrofurantoin (19.5%) showed the highest and lowest drug resistance, respectively. A total of 156 isolates (78%) were identified as ESBLs using the phenotypic method. Moreover, 76 (38%), 90 (45%), and 123 (61.5%) isolates consisted of blaCTX-M, blaSHV, and blaTEM, respectively. Conclusions: Overall, the findings of this study showed that blaTEM was the most common gene with a frequency of 61.5% in ESBL E. coli.

scholarly journals The Dissemination of Multidrug Resistance (MDR) and Extensively Drug Resistant (XDR) among Uropathogenic E. coli (UPEC) Isolates from Urinary Tract Infection Patients in Babylon Province, Iraq

2019 ◽  
Vol 16 (4(Suppl.)) ◽  
pp. 0986
Author(s):  
Al-Hasnawy Et al.
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Extensively Drug Resistant

Antibiotic resistance is a problem of deep scientific concern both in hospital and community settings. Rapid detection in clinical laboratories is essential for the judicious recognition of antimicrobial resistant organisms. So, the growth of Uropathgenic Escherichia coli (UPEC) isolates with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that thwart therapy for (UTIs) has been detected and has straight squeezed costs and extended hospital stays. This study aims to detect MDR- and XDR-UPEC isolates. Out of 42 UPEC clinical isolates were composed from UTI patients. The bacterial strains were recognized by standard laboratory protocols. Susceptibility to antibiotic was measured by the standard disk diffusion method Out of 42 Uropathogenic E. coli, 37 (88.09%) were found to be MDR while 5 isolates (11.90%) were XDR. The present study concluded high prevalence of uropathogenic Escherichia coli (UPEC) with Multidrug-resistant (MDR) isolated from urinary tract infection in Babylon province – Iraq.

scholarly journals Bacterial Isolates and Antibiotic Sensitivity Pattern from Urinary Tract Infection in a Private Diagnostic Laboratory in Dhaka, Bangladesh

2020 ◽  
Vol 37 (2) ◽  
pp. 56-60
Author(s):  
Md Habibur Rahman ◽  
Md Mushtaque Ahmed ◽  
Dayanidhi Sarkar ◽  
Md Asadur Rahman
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Agents ◽  
Standard Procedure ◽  
Antibiotic Sensitivity ◽  
Urine Samples ◽  
Diagnostic Laboratory ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

Urinary tract infection (UTI) is a common infection encountered by clinicians and despite the widespread availability of antimicrobial agents, UTI has become difficult to treat because of appearance pathogens with increasing resistance to antimicrobial agents. The objectives of this study were to determine the pathogens causing UTI and to determine the antibiotic sensitivity status among these isolates in a diagnostic laboratory in Dhaka city. A laboratory based cross sectional survey was conducted in a diagnostic Centre in Dhaka Bangladesh from July 2016 to December 2016. A total of 553 urine samples were collected from each patients and processed in microbiology laboratory to isolate pathogens and antibiotic susceptibility test using standard procedure. Among 553 urine samples, 158 (28.57%) samples was found to be culture positive of which 39 (24.70%) were isolated from male patients and 119 (75.30%) from female patients. Escherichia coli (43.67%) were found to be the predominant pathogen followed by Staphylococcus spp. (16.45%), Enterococcus spp. (13.39%), Klebsiella spp. (13.29%), Candida spp. (5.70%), Acinetobacter spp. (4.43%), Pseudomonas spp. (3.80%) and Proteus spp. (1.27%). The carbapenem group antibiotics (imipenem and meropenem) was found to be resistant in 0 to 5.1% of the Gram negative isolates. On the Other hand, most of the Gram positive isolates showed sensitivity to linezolid and vancomycin. This study showed that E. coli isolates were the predominant pathogens and showed resistance to commonly prescribed drugs resulting in a very few options for drug to treat UTIs. Bangladesh J Microbiol, Volume 37 Number 2 December 2020, pp 56-60

ANTIBIOTIC SUSCEPTIBILITY PATTERN OF ESCHERICHIA COLI ISOLATED FROM CHILDREN WITH URINARY TRACT INFECTION

2021 ◽  
pp. 152-157
Author(s):  
YOGESH OLI ◽  
GANESH BHANDARI ◽  
UPASHANA BHANDARI ◽  
SUNITA BISTA ◽  
AMRIT KUMAR BHATTARAI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Disk Diffusion Method ◽  
Antibiotic Susceptibility Pattern ◽  
E Coli

Objective: This work aimed to detect the antibiotic susceptibility pattern of Escherichia coli isolated from children, as it is the most predominant pathogen of urinary tract infection (UTI). Methods: About 530 urine samples were collected and tested using the modified Kirby–Bauer disk diffusion method to find the susceptibility pattern of isolated bacteria. Results: Out of a total of 530 samples, 114 (21.50%) showed significant growth. A total of 8 different types of bacteria were isolated from the growth of positive samples. Among the isolates, E. coli 66 (57.8%) was found to be the most predominant organism followed by Klebsiella pneumoniae 18(15.8%), Proteus spp. 10 (8.8%), Staphylococcus aureus 8 (7.0%), Acinetobacter spp. 4 (3.5%), CoNS 4 (3.5%), Enterobacter spp. 2 (1.8%), and Pseudomonas aeruginosa 2 (1.8%). In the present study, out of 66 E. coli, 37 (56.1%) were multidrug-resistant strain. E. coli showed 94.0% resistance to ceftriaxone followed by ceftazidime 86.5% and cefotaxime 70.3%. Imipenem (91.9%) followed by amikacin (89.2%) seems to be the effective drug against UTI causing E. coli in children. Conclusion: Multidrug resistance may possess difficulties with the choice of therapeutic options for the treatment of severe infections.

scholarly journals Asymptomatic Bacteriuria among Pregnant Women Attending a Tertiary Care Hospital in Western Nepal: A Cross-sectional Prospective Study

2021 ◽  
Vol 9 (01) ◽  
pp. 38-42
Author(s):  
Shristi Raut ◽  
Sulochana Khatiwada ◽  
Narayan GC
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Asymptomatic Bacteriuria ◽  
Urine Samples ◽  
Diffusion Method ◽  
Care Hospital ◽  
Significant Bacteriuria

INTRODUCTION Urinary tract of female undergoes tremendous changes during pregnancy which increases their risk of acquiring infection. Asymptomatic bacteriuria is a common entity among pregnant women which refers to significant bacteriuria (>105 bacteria per ml of urine) without any typical symptoms of urinary tract infection. Asymptomatic bacteriuria can lead to various maternal and fetal complications if not detected and treated on time. MATERIAL AND METHODS Total 280 urine samples were collected in sterile universal containers from pregnant women not showing typical symptoms of urinary tract infection at the time of sample collection. Urine samples were inoculated in both MacConkey agar and Blood agar by semi quantitative culture method. Culture plates were reported after 24 hours of incubation at 370C. Bacteria isolated were subjected to antibacterial susceptibility testing by modified Kirby-Bauer disc diffusion method. RESULTS Out of 280 urine cultures, 213 samples were sterile. Significant bacteriuria was seen in 25 cases (8.9%) followed by insignificant bacteriuria (20, 7.14%) and contamination (17, 6.10%). Highest number of cultures positive were in age group 21-30 years (19, 9.1%,). Out of 25 cases of significant bacteriuria, 60% were primigravida and 40% were multigravida. The most common organism isolated was Escherichia coli (10, 60%) followed by Klebsiella pneumoniae (5, 40%). CONCLUSION Screening of all pregnant women for asymptomatic bacteriuria is essential during their antenatal checkup. Escherichia coli is the commonest organism that cause asymptomatic bacteriuria. Appropriate antibiotic therapy is absolutely necessary for positive cases on the basis of antimicrobial susceptibility result to prevent unwanted obstetric complications.

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